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学者姓名：齐国敏
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Abstract ：
DNA材料具有的特异性、可编程性和生物相容性，使得其在生物检测和药物递送方面具有很大的应用优势。为了进一步拓展DNA材料的应用和合成方法，本文报道了利用DNA与金属离子之间的配合作用，简单高效地制备具有肿瘤标志物micro RNA-21(mi R-21)响应性的Zn~(2+)/DNA自组装纳米粒子（ZDNPs）。通过粒子中DNA发卡结构与mi R-21的特异性互补，激活荧光信号并释放Zn~(2+)，导致细胞内产生大量活性氧，从而用于肿瘤的荧光成像和氧化应激治疗。合成的ZDNPs为形状均匀的球形粒子，能有效对Zn~(2+)进行装载。通过ZDNPs对mi R-21的响应性实验，验证了ZDNPs与miR-21浓度之间具有良好的线性响应荧光信号，线性响应范围在5～160 nmol/L，检测限为5 nmol/L，且具备特异性。此外，本文对ZDNPs在细胞内的作用效果也进行了研究，结果表明其能在肿瘤细胞内进行响应性的荧光成像，并能通过氧化应激途径介导肿瘤细胞凋亡。在活体的荧光成像和肿瘤治疗中，ZDNPs在肿瘤病灶部位体现出特异性和持续性的示踪能力，并且对肿瘤产生了明显的生长抑制效果。
Keyword ：
DNA发卡 DNA发卡 microRNA-21 microRNA-21 氧化应激治疗 氧化应激治疗 肿瘤成像 肿瘤成像 锌离子 锌离子
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| GB/T 7714 | 徐昕 , 李烨 , 王敏 et al. 基于microRNA-21响应的Zn~(2+)/DNA自组装体用于肿瘤的检测和氧化应激治疗 [J]. | 应用化学 , 2024 , 41 (01) : 164-174 . |
| MLA | 徐昕 et al. "基于microRNA-21响应的Zn~(2+)/DNA自组装体用于肿瘤的检测和氧化应激治疗" . | 应用化学 41 . 01 (2024) : 164-174 . |
| APA | 徐昕 , 李烨 , 王敏 , 傅章程 , 齐国敏 , 卢春华 . 基于microRNA-21响应的Zn~(2+)/DNA自组装体用于肿瘤的检测和氧化应激治疗 . | 应用化学 , 2024 , 41 (01) , 164-174 . |
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Electrochemiluminescence (ECL) of graphitic carbon nitride nanosheets (CNNSs) is showing extensive interest to researchers. However, the poor ECL stability of CNNSs has always been an urgent problem, which severely limits its application. Herein, a systemic investigation is carried out for the exact mechanism affecting the ECL stability of CNNSs. It is found experimentally that dissolved oxygen in the solution is selectively reduced into H2O2 through a two-electron pathway on the surface of CNNSs. The enriched electrogenerated H2O2 on the electrode surface leads to the progressive inhibition of ECL of CNNSs due to its competition with negatively charged CNNSs (CNNSs center dot-) for sulfate radical ions (SO4 center dot-). On the basis, the pathway of eliminating O2 by N2, composing CNNSs with other nanomaterials to realize the four-electron reduction, or improving the concentration of K2S2O8 is proved to be effective means for improving ECL stability successfully. Proofing the concept of CNNSs with high ECL stability, an ECL sensor is developed for the detection of H2O2. This work not only exploits the intrinsic mechanism of poor ECL stability of CNNSs, but also provides efficient methods to improve the ECL stability. Thus, we believe it has promising attraction in the research of CNNSs-based assays.
Keyword ：
Electrochemiluminescence Electrochemiluminescence Graphitic carbon nitride nanosheets Graphitic carbon nitride nanosheets Luminous stability Luminous stability Mechanism Mechanism
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| GB/T 7714 | Fu, Xiaolong , Huang, Ju , Lai, Xiaojing et al. Strategy and mechanism for strong and stable electrochemiluminescence of graphitic carbon nitride [J]. | ELECTROCHIMICA ACTA , 2023 , 444 . |
| MLA | Fu, Xiaolong et al. "Strategy and mechanism for strong and stable electrochemiluminescence of graphitic carbon nitride" . | ELECTROCHIMICA ACTA 444 (2023) . |
| APA | Fu, Xiaolong , Huang, Ju , Lai, Xiaojing , Rong, Jiefeng , Qi, Guomin , Lin, Zhenyu et al. Strategy and mechanism for strong and stable electrochemiluminescence of graphitic carbon nitride . | ELECTROCHIMICA ACTA , 2023 , 444 . |
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A novel three-dimensional covalent organic framework (3D-COF) with content-tunable and active hydroxyl groups (OH) on the pore walls was developed and adopted for the high-performance capture of okadaic acid (OA) marine toxins. Using pore-surface engineering, the integration of linear building blocks (4,4'-diamino-3,3'- biphenyldiol, BD(OH)2 and benzidine, BD) with the 3D structural building block backbone (4,4',4',4'''-methane-tetrayltetrabenzaldehyde, TFPM) was achieved. By adjusting the ratio of BD(OH)2, functional multicomponent-COFs [OH](x)-BD-TFPM COFs (X = 25%) were synthesized, which offered ideal access to convert a conventional COF into a functional platform with multiple-mode interactions of hydrophobic and hydrophilic groups for OA capture. [OH](x)-BD-TFPM was characterized using SEM, XRD, FT-IR, and BET. The adsorption features and analytical performance of OA were screened and evaluated. Optimization of dispersive solid-phase extraction using [OH]25-BD-TFPM was accomplished, and the method was verified for sensitive quantitative detection of OA in clam and mussel samples. Coupled with LC-MS/MS, the resultant [OH]25-BD-TFPM COF demonstrated the ability to analyze OA, and the limit of detection for OA in shellfish was determined to be 0.005 mu g/kg. A significant improvement in trace OA detection was observed compared to previously reported SPE materials without adjustable hydrophilic interactions. The recoveries of OA in the fortified clam and mussel samples were in the ranges of 93.9-105.1% and 96.7-110.2%, respectively. This study highlights that OH-group surface engineering in channel walls is a facile and powerful strategy for developing functional 3D-COFs with multiple interactions for high-performance target capture.
Keyword ：
Hydroxyl functionalization Hydroxyl functionalization Okadaic acid Okadaic acid Shellfish toxin Shellfish toxin Surface engineering Surface engineering Three-dimensional covalent organic frameworks Three-dimensional covalent organic frameworks
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| GB/T 7714 | Qi, Guomin . Efficient capture and highly sensitive analysis of okadaic acid by three-dimensional covalent organic frameworks with hydroxyl surface engineering [J]. | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1708 . |
| MLA | Qi, Guomin . "Efficient capture and highly sensitive analysis of okadaic acid by three-dimensional covalent organic frameworks with hydroxyl surface engineering" . | JOURNAL OF CHROMATOGRAPHY A 1708 (2023) . |
| APA | Qi, Guomin . Efficient capture and highly sensitive analysis of okadaic acid by three-dimensional covalent organic frameworks with hydroxyl surface engineering . | JOURNAL OF CHROMATOGRAPHY A , 2023 , 1708 . |
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基于单糖与1-苯基-3-甲基-5-吡唑啉酮的柱前衍生，采用反相高效液相色谱（HPLC）梯度洗脱/紫外检测联用技术，建立了凉粉草多糖水解单糖的HPLC指纹图谱，用于凉粉草水提溶液中多糖水解单糖组分分析，鉴别测定了水解单糖组分中核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖等8种单糖含量，开展了不同地区的凉粉草多糖水解单糖的HPLC指纹图谱相似度分析、聚类分析和主成分分析。研究表明，不同地区凉粉草水解单糖组分中半乳糖、半乳糖醛酸和葡萄糖是样本聚类的重要变量，地区差异导致聚类分析差异，不同产地的凉粉草中多糖水解产生的单糖组分含量和分布具有一定的差异。方法可为凉粉草水提溶液中多糖水解单糖组分的评价提供一定的技术支持。
Keyword ：
HPLC HPLC 凉粉草 凉粉草 单糖 单糖 多糖 多糖 指纹图谱 指纹图谱
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| GB/T 7714 | 齐国敏 , 曹华斌 , 林旭聪 . 凉粉草多糖水解单糖成分的高效液相色谱指纹图谱分析 [J]. | 分析试验室 , 2023 , 42 (04) : 501-506 . |
| MLA | 齐国敏 et al. "凉粉草多糖水解单糖成分的高效液相色谱指纹图谱分析" . | 分析试验室 42 . 04 (2023) : 501-506 . |
| APA | 齐国敏 , 曹华斌 , 林旭聪 . 凉粉草多糖水解单糖成分的高效液相色谱指纹图谱分析 . | 分析试验室 , 2023 , 42 (04) , 501-506 . |
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Based on the pre-column derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolonethe fingerprints of polysaccharide hydrolysate monosaccharides in the extract of Mesona chinensis Benth was established using reversed-phase high performance liquid chromatography HPLC gradient elution/UV detection technologywhich was used for the analysis of the components and content of polysaccharide hydrolyzed monosaccharides in the aqueous extract of Mesona chinensis Benthand eight monosaccharide components including riboserhamnoseglucuronic acidgalacturonic acidglucosegalactosexylose and arabinose were identified. Similarity analysiscluster analysisprincipal component analysis of the HPLC fingerprints of monosaccharide components were carried outand the changes in the contents of galactose galacturonic acid and glucose monosaccharide components in various samples were clarified. Regional differences lead to differences in cluster analysisand there are certain differences in the content and distribution of monosaccharide components produced by polysaccharide hydrolysis in Mesona chinensis Benth collected from different origins. The method can provide a potential technology for characterizing monosaccharide components of the polysaccharide in aqueous extract of Mesona chinensis Benth. © 2023, Youke Publishing Co.,Ltd. All rights reserved.
Keyword ：
Cluster analysis Cluster analysis Column chromatography Column chromatography Extraction Extraction Glucose Glucose High performance liquid chromatography High performance liquid chromatography Hydrolysis Hydrolysis Palmprint recognition Palmprint recognition Principal component analysis Principal component analysis
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| GB/T 7714 | Qi, Guomin , Cao, Huabin , Lin, Xucong . High performance liquid chromatography fingerprints of polysaccharide hy drolysate monosaccharides in Mesona chinensis Benth [J]. | Chinese Journal of Analysis Laboratory , 2023 , 42 (4) : 501-506 . |
| MLA | Qi, Guomin et al. "High performance liquid chromatography fingerprints of polysaccharide hy drolysate monosaccharides in Mesona chinensis Benth" . | Chinese Journal of Analysis Laboratory 42 . 4 (2023) : 501-506 . |
| APA | Qi, Guomin , Cao, Huabin , Lin, Xucong . High performance liquid chromatography fingerprints of polysaccharide hy drolysate monosaccharides in Mesona chinensis Benth . | Chinese Journal of Analysis Laboratory , 2023 , 42 (4) , 501-506 . |
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MC-LR possessing strong hepatotoxicity, potential carcinogenicity, and biological toxicity could have an intensive influence on human health and the aquatic ecological environment and need to be monitored critically. For the specific and highly sensitive analysis performance, agold-magnetic composite nanoparticlemodified by aptamer-cDNAfluorescent hybridization probe (Fe3O4@ MIL-101 -NH2 @Au@aptamer) was prepared and applied for sensing detection of MC-LR in water with laser-induced fluorescence (LIF). This work studied the feasibility, optimization of analysis conditions, specificity, stability of the method, and sample analysis application. As a result, a high Brunauer-Emmett-Teller (BET) specific surface area of 114. 02 m(2) center dot g(-1) was achieved in Fe3O4@ MIL-101-NH2@ AuNPs, which resulted in a high modification rate of up to 98. 8%0 achieved for aptamercDNA fluorescenthybridization probe. With the excitation wavelength of 497 nm, the aptamer-cDNA fluorescent probe has a strong fluorescence response with the emission wavelength of 512 nm to the trace MC-LR. Under the optimal conditions (pH 7. 5, NaC1 concentration 500 mmol center dot L-1 nanogold size 20 nm, analytical time 30 min), a good linear relationship between MCLR concentration and fluorescence intensity was achieved in a wide linear range (0. 020 similar to 3. 000 mu g center dot L-1) with the limit of detection (LOD) as low as 0. 006 mu g center dot L-1, which was 1. 6 similar to 22. 3 folds better than that of the most fluorescence methods reported previously. The relative standard deviation (RSD) of intra-day, intra-day, and inter-batch was gained in 1. 7%similar to 8. 8%, and the relative error (RE) of -4. 3%similar to 4. 1%0. High specific selectivity and low cross-reactivity for MC-LR (1. 0 mu g center dot L-1) were also achieved using theas-prepared fluorescent probe, even if the concentration of interfering toxins (MC-RR, MCYR, OA) was 100 folds higher than that of MC-LR. The fluorescence responses of MC-LR in these mixtures werewell consistent with that of MC-LR in the standard solution. Applied to the analysis of samples from the Minjiang River, Lake Water, and the inland river, the identification of MC-LR in water samples was satisfactory. The recovery yields of MC-LR for three concentrations (0. 050, 0. 100 and 1. 000 mu g center dot L-1) rangedin (90. 1%+/- 0 6. 4%) (104. 2%+/- 7. 0%) (n=3). The results were consistent with those obtained by the LC-MS method [(91. 3%+/- 0 7. 0%) (104. 4%+/- 2. 0%), n=3]. It might light a new technique for the specific and rapid monitoring technology for tracing MC-LR in the environmental water.
Keyword ：
Aptamer Aptamer Hybridization probe Hybridization probe Laser induced fluorescence Laser induced fluorescence Magnetic nanoparticle Magnetic nanoparticle MC-LR MC-LR
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| GB/T 7714 | Qi Guo-min , Tong Shi-qian , Lin Xu-cong . Specific Identification of Microcystin-LR by Aptamer-Functionalized Magnetic Nanoprobe With Laser-Induced Fluorescence [J]. | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2023 , 43 (12) : 3813-3819 . |
| MLA | Qi Guo-min et al. "Specific Identification of Microcystin-LR by Aptamer-Functionalized Magnetic Nanoprobe With Laser-Induced Fluorescence" . | SPECTROSCOPY AND SPECTRAL ANALYSIS 43 . 12 (2023) : 3813-3819 . |
| APA | Qi Guo-min , Tong Shi-qian , Lin Xu-cong . Specific Identification of Microcystin-LR by Aptamer-Functionalized Magnetic Nanoprobe With Laser-Induced Fluorescence . | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2023 , 43 (12) , 3813-3819 . |
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以垃圾焚烧飞灰与市政污泥为研究对象，探讨了飞灰与市政污泥混合物陶粒烧结温度和时间条件，开展了陶粒烧结物的形貌结构、物相分析及其重金属形态分布和含量变化。研究表明：在优化条件下，飞灰-市政污泥混合物在1150℃温度下烧结成型良好；陶粒烧结物中具有新矿物相生成，如CaAlSiO_4(OH)、CaSiO_3和CaAl_4O_7等矿物相，化学形态由不稳定形态转变为稳定形态；高温烧结后重金属Ba较为稳定的可氧化态F_3和残渣态F_4比例大幅度提高，其中残渣态F4所占比例提高到79%;Zn、Pb、Cu和Cr等金属可氧化态F_3可氧化态和残渣态F_4占比也得到了明显提高；Zn、Pb、Cu和Cr浸出浓度分别降低至0.33、0.003、0.03和0.01 mg/L，低于危险废弃物有害金属浸出浓度的最大允许限量。
Keyword ：
市政污泥 市政污泥 形态分布 形态分布 焚烧飞灰 焚烧飞灰 重金属 重金属 陶粒 陶粒
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| GB/T 7714 | 齐国敏 . 飞灰-污泥烧结物中重金属分布及形态分析 [J]. | 化学工程与装备 , 2023 , 5 (10) : 230-234 . |
| MLA | 齐国敏 . "飞灰-污泥烧结物中重金属分布及形态分析" . | 化学工程与装备 5 . 10 (2023) : 230-234 . |
| APA | 齐国敏 . 飞灰-污泥烧结物中重金属分布及形态分析 . | 化学工程与装备 , 2023 , 5 (10) , 230-234 . |
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微囊藻毒素-LR(MC-LR)具有强烈的肝毒性、致癌性和发育毒性，亟需严格监测。为实现环境水体中微痕量MC-LR的快速特异灵敏的分析识别，以金属有机框架化合物(MOF)为介导，增强磁纳米粒子比表面积，高效负载纳米金和适配体-cDNA分子杂交荧光探针，研发新型的适配体功能化磁纳米荧光探针(Fe_3O_4@MIL-101-NH_2@Au@aptamer),实现了对MC-LR的适配体特异识别-激光诱导荧光(LIF)超高灵敏分析。论文详细研究了适配体磁纳米探针荧光检测MC-LR的可行性、 MC-LR测定的优化条件及其方法性能。结果表明：MOF修饰的磁纳米颗粒Fe_3O_4@MIL-101-NH_2的Brunauer-Emmett-Teller(BET)比表面积达到114.02 m~2·g~(-1),比单一Fe_3O_4提高了近5倍，适配体-cDNA杂交荧光探针在磁纳米颗粒表面的修饰率达98%以上，适配体磁纳米探针对水体中痕量MC-LR具有很强的荧光响应。在最优条件下(pH 7.5、 NaCl浓度500 mmol·L~(-1)、纳米金尺寸为20 nm,测定时间为30 min),适配体磁纳米探针与MC-LR结合释放出荧光互补链，体系荧光强度与MC-LR含量成正比，线性浓度范围为0.020～3.000μg·L~(-1),检出限(LOD)为0.006μg·L~(-1),灵敏度比文献报道的荧光分析法提高了1.6～22.3倍。所建立的适配体磁纳米探针-LIF法对MC-LR的识别特异性高，在100倍量的干扰物(微囊藻毒素MC-RR、 MC-YR、大田软海绵酸OA)共存情况下，适配体磁纳米探针在混合体系中的荧光响应与在MC-LR单样中的响应强度偏差小于3.3%,交叉反应性小；日内、日间和批间的测定标准偏差(RSD)为1.7%～8.8%,相对误差RE为-4.3%～4.1%,方法稳定性和重现性好。该方法应用于闽江、西湖水和内河水等样品分析，水中MC-LR得到了良好的识别检出，不同加标浓度的MC-LR(0.050, 0.100和1.000μg·L~(-1))测定回收率为(90.1%±6.4%)～(104.2%±7.0%)(n=3),与LC-MS确证方法的测定结果[加标回收率(91.3%±7.0%)～(104.4%±2.0%),n=3]一致。所建立的适配体磁纳米探针与LIF联用技术对水中痕量MC-LR具有高的特异识别和灵敏检出能力，为环境水中痕...
Keyword ：
微囊藻毒素-LR 微囊藻毒素-LR 杂交探针 杂交探针 激光诱导荧光 激光诱导荧光 磁纳米颗粒 磁纳米颗粒 适配体 适配体
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| GB/T 7714 | 齐国敏 , 童诗谦 , 林旭聪 . 适配体磁纳米探针-激光诱导荧光特异识别微囊藻毒素-LR [J]. | 光谱学与光谱分析 , 2023 , 43 (12) : 3813-3819 . |
| MLA | 齐国敏 et al. "适配体磁纳米探针-激光诱导荧光特异识别微囊藻毒素-LR" . | 光谱学与光谱分析 43 . 12 (2023) : 3813-3819 . |
| APA | 齐国敏 , 童诗谦 , 林旭聪 . 适配体磁纳米探针-激光诱导荧光特异识别微囊藻毒素-LR . | 光谱学与光谱分析 , 2023 , 43 (12) , 3813-3819 . |
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Herein, a vinylene-linked covalent organic framework (TMT-BPDA-COF) was prepared via the Aldol condensation of 2,4,6-trimethyl-1,3,5-triazine (TMT) and 4,4'-biphenyl dimethyl formaldehyde (BPDA). TMT-BPDACOF displayed high crystallinity and thermal stability, and robust chemical stability in strong acid, strong base and common organic solvents. TMT-BPDA-COF nanosheets emitted strong yellow fluorescence with the quantum yield of 14.9% and showed fast reversible response toward pH with a wide range (pH 0.0 - 14.0) based on the intramolecular charge transfer effect. Furthermore, portable fluorescent sensors were prepared by incorporated TMT-BPDA-COF nanosheets into agarose gel and cellulose acetate butyrate (CAB), respectively. TMT-BPDA-COF gel showed a wide pH response range over pH 0.0 - 14.0 with a linear range of pH 4.0 - 14.0. TMT-BPDA-COF CAB film exhibited high sensitivity for sensing of NH3 gas with a limit of detection of 1.6 ppb. TMT-BPDA-COF agarose gel and CAB film also exhibited good reversibility and reproducibility in response to pH and NH3 gas, respectively. We expect TMT-BPDA-COF-based sensors, which feature fast response, high sensitivity, wide response range, good reproducibility and reversibility, can apply to detect pH and NH3 gas in the field of environment and industry.
Keyword ：
Covalent organic frameworks Covalent organic frameworks Films Films Fluorescent sensors Fluorescent sensors Gels Gels NH3 NH3 PH PH
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| GB/T 7714 | Cai, Jiaming , Qiu, Yonghan , Qi, Guomin et al. A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 402 . |
| MLA | Cai, Jiaming et al. "A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range" . | SENSORS AND ACTUATORS B-CHEMICAL 402 (2023) . |
| APA | Cai, Jiaming , Qiu, Yonghan , Qi, Guomin , Guo, Liangqia . A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 402 . |
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基于柱前衍生-高效液相色谱/紫外检测技术，建立了多糖水解单糖的HPLC分析技术，用于凉粉草提取液多糖水解单糖组分分析和含量测定。采用1-苯基-3-甲基-5-吡唑啉酮（PMP）衍生，优化并获得了单糖组分的衍生条件和HPLC梯度洗脱分析流程，鉴别了核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖等8种单糖；应用于18种凉粉草提取液样品分析，凉粉草多糖水解单糖以半乳糖醛酸、葡萄糖和半乳糖为主，含量分别为2.50～20.48μg/mL、2.97～38.52μg/mL、2.72～89.32μg/mL。该方法操作简单、准确，可为凉粉草多糖提取液水解单糖组分特征评价与定量分析提供技术。
Keyword ：
凉粉草多糖 凉粉草多糖 单糖 单糖 柱前衍生 柱前衍生 紫外检测 紫外检测 高效液相色谱 高效液相色谱
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| GB/T 7714 | 齐国敏 . 高效液相色谱-紫外检测分析凉粉草多糖水解单糖组分 [J]. | 福建分析测试 , 2022 , 31 (03) : 28-33 . |
| MLA | 齐国敏 . "高效液相色谱-紫外检测分析凉粉草多糖水解单糖组分" . | 福建分析测试 31 . 03 (2022) : 28-33 . |
| APA | 齐国敏 . 高效液相色谱-紫外检测分析凉粉草多糖水解单糖组分 . | 福建分析测试 , 2022 , 31 (03) , 28-33 . |
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