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学者姓名：王伟
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Abstract ：
Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant. Capillary electrophoresis is a versatile separation means. It has different separation modes, so, it can be applied in the analysis of both bacteria and flavonoids. In this study, an efficient method of indirect separation and detection of three bacteria Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus was developed. The determination of bacteria was realized by separating the aptamers corresponding to the bacteria by capillary sieving electrophoresis. This method avoided the disadvantages of low resolution, channel adsorption and bacterial aggregation, encountered in the direct separation of bacteria. The separation of the flavonoid kaempferide was realized by capillary zone electrophoresis, and kaempferide in Kaempferia galanga was determined. Kaempferide and the extract of Kaempferia galanga were applied in the antibacterial tests separately. Using the developed method, the bacterial growth curves and the curves of the kaempferide concentration were studied respectively. The results showed that both kaempferide and Kaempferia galanga could effectively inhibit the bacteria. The inhibitory effect on the terrestrial bacteria Escherichia coli, and Staphylococcus aureus was better than that on the marine bacterium Vibrio parahaemolyticus.
Keyword ：
capillary electrophoresis capillary electrophoresis flavonoids flavonoids foodborne foodborne indirect separation indirect separation kaempferide kaempferide pathogenic bacteria pathogenic bacteria
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| GB/T 7714 | Wang, Qingqing , Ye, Junlan , Wang, Wei . Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis [J]. | ELECTROPHORESIS , 2025 . |
| MLA | Wang, Qingqing 等. "Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis" . | ELECTROPHORESIS (2025) . |
| APA | Wang, Qingqing , Ye, Junlan , Wang, Wei . Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis . | ELECTROPHORESIS , 2025 . |
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Abstract ：
Calcite is a promising material choice for adsorbing phosphates because of its abundance and environmentally benign nature. However, the slow adsorption kinetics and hence low adsorption capacity within a short time frame hinders its practical application. In this work, we solve these problems by presenting a low Mg2+-doped calcite adsorbent, Mg-10. With a 3.75 wt% of Mg2+ doping, Mg-10 exhibits a remarkable adsorption capacity of 157.7 mg P/g. It also demonstrates a substantial boost in the adsorption kinetics, achieving a sixfold increase in adsorption capacity within 24 h compared to the undoped calcite. Meanwhile, Mg-10 not only offers improved adsorption selectivity but also maintains a stable effluent pH, underscoring its environmental compatibility. By conducting soil column experiments, we find that Mg-10 quickly captures the excess phosphates during the mimicking fertilization process, and slowly releases the nutrient afterwards, which can increase the feralization efficiency. These results provide alternative strategies for managing phosphate pollution originated from fertilization, and underscores the potential of Mg-10 in sustainable agriculture and environmental remediation.
Keyword ：
Adsorbents Adsorbents Calcite Calcite Fertilization Fertilization Mg-doped Mg-doped Phosphate removal Phosphate removal
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| GB/T 7714 | Wang, Yi-Fan , Wang, Zuo-Bei , Zhang, Yong-Hui et al. Boosting the phosphate adsorption of calcite by low Mg2+-Doping [J]. | ENVIRONMENTAL RESEARCH , 2025 , 267 . |
| MLA | Wang, Yi-Fan et al. "Boosting the phosphate adsorption of calcite by low Mg2+-Doping" . | ENVIRONMENTAL RESEARCH 267 (2025) . |
| APA | Wang, Yi-Fan , Wang, Zuo-Bei , Zhang, Yong-Hui , Huang, You-Gui , Ye, Xin , Wang, Wei . Boosting the phosphate adsorption of calcite by low Mg2+-Doping . | ENVIRONMENTAL RESEARCH , 2025 , 267 . |
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To determine multiple microRNAs (miRNAs) from cells simultaneously is essential for understanding biological functions. Capillary electrophoresis (CE) can simultaneously determine multiple miRNAs by separation. Nevertheless, similar lengths and low concentrations in cells make miRNAs hard to separate and detect. In this study, CE with laser-induced fluorescence detection was combined with catalytic hairpin assembly (CHA) to determine three miRNAs, miR-21, miR-31, and miR-122. The amplification products of CHA, which were DNA duplexes, were designed to have different lengths for different miRNAs. This allowed for easy separation of the duplexes of different miRNAs by CE. The indirect determination of miRNAs was then achieved by separating and detecting these duplexes. A magnetic field was first applied on the capillary sieving electrophoresis to assist in the separation of the duplexes. Under the optimal conditions, the three duplexes could be completely separated within 2.5 min with the detection limits of miRNAs in the range 1.12-4.05 x 10-15 M. MiR-21 and miR-31 were successfully determined from Hela cells, while miR-122 was determined from chicken livers by this method. The recoveries ranged from 97.5% to 118%. The developed method was sensitive and reliable for miRNA determination.
Keyword ：
capillary electrophoresis capillary electrophoresis catalytic hairpin assembly catalytic hairpin assembly indirect determination indirect determination magnetic field assistance magnetic field assistance microRNA microRNA
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| GB/T 7714 | Ning, Jinfeng , Ye, Junlan , Wang, Qingqing et al. Indirect and sensitive determination of microRNAs by magnetic field-assisted capillary sieving electrophoresis combined with catalytic hairpin assembly [J]. | JOURNAL OF SEPARATION SCIENCE , 2024 , 47 (14) . |
| MLA | Ning, Jinfeng et al. "Indirect and sensitive determination of microRNAs by magnetic field-assisted capillary sieving electrophoresis combined with catalytic hairpin assembly" . | JOURNAL OF SEPARATION SCIENCE 47 . 14 (2024) . |
| APA | Ning, Jinfeng , Ye, Junlan , Wang, Qingqing , Wang, Wei . Indirect and sensitive determination of microRNAs by magnetic field-assisted capillary sieving electrophoresis combined with catalytic hairpin assembly . | JOURNAL OF SEPARATION SCIENCE , 2024 , 47 (14) . |
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An eight-sided prism sample, obtained from a hot-rolled AZ31 magnesium alloy sheet, was compressed at room temperature along the transverse direction to investigate the influence of local strain on twinning behavior using electron backscatter diffraction (EBSD) measurements, hardness distribution, and metallographic observations. The octagonal surface of the sample was divided into distinct regions based on hardness distribution and metallographic observations. Combined analysis of the Schmid factor (SF) and the strain compatibility factor (m') was employed to study twin variant selection. Basal on SF ratio distribution, the Schmid factor criterion, can predict over 75% of observed twin variants in regions A and D (normal stress samples). In contrast, 64% of twin variant selection behavior in region C (shear stress sample) can be effectively explained using a pure shear model. Twin variants with high strain compatibility factors may prefer activation to reduce stress concentration. The strain compatibility factor is more appropriate than the Schmid factor for analyzing the effect of local strain on the selection behavior of twin variants.
Keyword ：
AZ31 magnesium alloy AZ31 magnesium alloy local strain state local strain state Schmid factor Schmid factor strain compatibility factor strain compatibility factor twin variants selection twin variants selection
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| GB/T 7714 | Lu, Boqin , Wang, Wei , Yao, Jinyi et al. A Study of {10-12} Twinning Activity Associated with Stress State in Mg-3Al-1Zn Alloy during Compression [J]. | METALS , 2024 , 14 (5) . |
| MLA | Lu, Boqin et al. "A Study of {10-12} Twinning Activity Associated with Stress State in Mg-3Al-1Zn Alloy during Compression" . | METALS 14 . 5 (2024) . |
| APA | Lu, Boqin , Wang, Wei , Yao, Jinyi , Deng, Liping , Xiao, Lei , Wang, Bingshu . A Study of {10-12} Twinning Activity Associated with Stress State in Mg-3Al-1Zn Alloy during Compression . | METALS , 2024 , 14 (5) . |
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Self-assembly is an important strategy to construct supramolecular frameworks. Small change in the assembling units often leads to significant variations in the self-assembly structures. In the work, we obtain two supramolecular frameworks assembled by different [EuL2]3+ units (L = tris((5,6-dimethyl-1H-benzo[d]imidazol-2-yl) methyl) amine). One meso compound {[EuIIIL2]& sdot;(H2O)6 & sdot;Cl3} (Meso-EuL2) is assembled by an achiral meso[EuL2]3+ unit, forming a supramolecular three-dimensional framework through six-fold intermolecular pi-pi interactions. The other is a racemic compound {[EuIIIL2]& sdot;(CH3OH)5 & sdot;Cl3} (Rac-EuL2), assembled by two chiral rac[EuL2]3+ units predominantly via hydrogen bonding, leading to a layered supramolecular framework. The luminescent properties are different between Meso-EuL2 and Rac-EuL2, with Meso-EuL2 exhibiting more excimerlike emissions, which is likely caused by its stronger pi-pi interactions in the structure.
Keyword ：
Luminescent Luminescent pi interactions pi interactions Rare earth complex Rare earth complex Self -assembly Self -assembly Supramolecular framework Supramolecular framework
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| GB/T 7714 | Li, Can-Kui , Zhang, Hui , Wang, Bin et al. Supramolecular frameworks assembled by [EuL 2 ] 3+ units with different coordination configurations [J]. | JOURNAL OF MOLECULAR STRUCTURE , 2024 , 1312 . |
| MLA | Li, Can-Kui et al. "Supramolecular frameworks assembled by [EuL 2 ] 3+ units with different coordination configurations" . | JOURNAL OF MOLECULAR STRUCTURE 1312 (2024) . |
| APA | Li, Can-Kui , Zhang, Hui , Wang, Bin , Liu, Jin , Zhang, Dong-Hai , Chen, Ting et al. Supramolecular frameworks assembled by [EuL 2 ] 3+ units with different coordination configurations . | JOURNAL OF MOLECULAR STRUCTURE , 2024 , 1312 . |
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Foodborne bacteria threaten human's health. Capillary electrophoresis (CE) is a powerful separation means for the determination of bacteria. Direct separation of bacteria suffers from the shortages of low resolution, channel adsorption, and bacterial aggregation. In this work, a method of nucleic acid strand displacement was developed to indirect separate the bacteria by CE. DNA complexes, consisting of probes and aptamers, were mixed with the three bacteria Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The aptamers could specifically bond with bacteria and release the probes. Through the separation of the probes, the bacteria could be indirectly determined by CE. This method avoided the shortages of direct separation of bacteria. Under the optimized conditions, the three probes for the bacteria could be separated and detected within 2.5 min by high-speed CE with laser-induced fluorescence detection. The limits of detection for the bacteria were in the range 4.20 x 106 to 1.75 x 107 CFU/mL. Finally, the developed method was applied on the study of antagonism of the coexistent bacteria to reveal the relationship between them. Furthermore, the efficiency of bacteriostasis of three traditional Chinese medicines, Coptis chinensis, Schisandra chinensis, and honeysuckle, was also studied by this method.
Keyword ：
antagonism antagonism bacteriostasis bacteriostasis capillary electrophoresis capillary electrophoresis foodborne bacteria foodborne bacteria strand displacement strand displacement
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| GB/T 7714 | Zhang, Shaoyan , Wang, Qingqing , Ning, Jinfeng et al. Nucleic acid strand displacement for indirect determination of foodborne bacteria by capillary electrophoresis and its application in antagonism and bacteriostasis studies [J]. | ELECTROPHORESIS , 2023 , 45 (3-4) : 318-326 . |
| MLA | Zhang, Shaoyan et al. "Nucleic acid strand displacement for indirect determination of foodborne bacteria by capillary electrophoresis and its application in antagonism and bacteriostasis studies" . | ELECTROPHORESIS 45 . 3-4 (2023) : 318-326 . |
| APA | Zhang, Shaoyan , Wang, Qingqing , Ning, Jinfeng , Wang, Wei . Nucleic acid strand displacement for indirect determination of foodborne bacteria by capillary electrophoresis and its application in antagonism and bacteriostasis studies . | ELECTROPHORESIS , 2023 , 45 (3-4) , 318-326 . |
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Flavonoids are widely used in the treatment of various diseases due to their antioxidant, anti-inflammatory, anticancer and antiviral properties. Fluorescence detection is rarely applied for the determination of flavonoids because of their weak fluorescence. In this work, a method of fluorescence enhancement of flavonoids was firstly introduced by using sodium acetate for flavonoid derivatization. The study discovered that flavonoids, with a hydroxyl at the C3 position, had the ability to emit strong fluorescence after derivatization. Five flavonoids, kaempferide, galangin, isorhamnetin, kaempferol and quercetin, having a special structure, were selected, derivatized and analyzed by capillary electrophoresis with laser-induced fluorescence detection. Under the optimal conditions, the five flavonoids could be completely separated within 3 minutes. Good linear relationships were obtained for all analytes and the limits of detection for the five flavonoids were in the range of 1.18-4.67 x 10(-7) mol L-1. Finally, the method was applied to the determination of flavonoids in five traditional Chinese medicines: aster, chamomile, galangal, tangerine peel and cacumen biotae. Flavonoids were successfully found in all these medicines by the developed method. The recoveries were in the range of 84.2-111%. The method developed in this study was fast, sensitive and reliable for the determination of flavonoids.
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| GB/T 7714 | Zhang, Shaoyan , Ning, Jinfeng , Wang, Qingqing et al. Fluorescence enhancement of flavonoids and its application in ingredient determination for some traditional Chinese medicines by CE-LIF [J]. | ANALYTICAL METHODS , 2023 , 15 (24) : 2964-2970 . |
| MLA | Zhang, Shaoyan et al. "Fluorescence enhancement of flavonoids and its application in ingredient determination for some traditional Chinese medicines by CE-LIF" . | ANALYTICAL METHODS 15 . 24 (2023) : 2964-2970 . |
| APA | Zhang, Shaoyan , Ning, Jinfeng , Wang, Qingqing , Wang, Wei . Fluorescence enhancement of flavonoids and its application in ingredient determination for some traditional Chinese medicines by CE-LIF . | ANALYTICAL METHODS , 2023 , 15 (24) , 2964-2970 . |
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MicroRNAs (miRNAs) play important roles in physiological and pathological processes of cells. To develop a fast, simple and sensitive method to determine miRNAs is significant for miRNA studies. In this work, determination of microRNA-122 (miR-122) was achieved by laser-induced fluorescence (LIF) detection. A vial-LIF interface was first applied for sample analysis. A two-step amplification of the fluorescence signal for miR-122 was designed and realized by applying duplex-specific nuclease in the cleaving of two sensing probes. Under optimized conditions, the analysis of a miR-122 sample could be completed in less than 50 min. Only 10 mu L sample was required for each test and the detection limit for the method was 0.60 pM equal to 1.2 amol of miR-122 in 10 mu L solution. Lastly, the developed method was successfully applied to determine miR-122 in chicken and duck liver. The developed method was fast, selective, sensitive and sample-saving for the determination of miRNAs.
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| GB/T 7714 | Yu, Xiufeng , Zhang, Shaoyan , Wang, Wei . Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection [J]. | ANALYTICAL METHODS , 2022 , 14 (17) : 1715-1720 . |
| MLA | Yu, Xiufeng et al. "Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection" . | ANALYTICAL METHODS 14 . 17 (2022) : 1715-1720 . |
| APA | Yu, Xiufeng , Zhang, Shaoyan , Wang, Wei . Determination of microRNA-122 in hepatocytes by two-step amplification of duplex-specific nuclease with laser-induced fluorescence detection . | ANALYTICAL METHODS , 2022 , 14 (17) , 1715-1720 . |
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Developing light-harvesting materials with broad spectral response is of fundamental importance in full-spectrum solar energy conversion. We found that, when a series of earth-abundant metal (Cu, Co, Ni and Fe) salts are dissolved in coordinating solvents uniformly dispersed nanodots (NDs) are formed rather than fully dissolving as molecular species. The previously unrecognized formation of this condensed state is ascribed to spontaneous aggregation of molecular transition-metal-complexes (TMCs) via weak intermolecular interactions, which results in redshifted and broadened absorption into the NIR region (200-1100 nm). Typical photoredox reactions, such as carbonylation and oxidative dehydrogenation, well demonstrate the feasibility of efficient utilization of NIR light (lambda>780 nm) by TMCs NDs. Our finding provides a conceptually new strategy for extending the absorption towards low energy photons in solar energy harvesting and conversion via photoredox transformations.
Keyword ：
Carbonylation Carbonylation Full-Spectrum-Light Response Full-Spectrum-Light Response Molecules Aggregation Molecules Aggregation Nanodots Nanodots Transition Metal Complexes Transition Metal Complexes
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| GB/T 7714 | Wang, Lele , Sa, Rongjian , Wei, Yingcong et al. Near-Infrared Light-Driven Photoredox Catalysis by Transition-Metal-Complex Nanodots [J]. | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION , 2022 , 61 (39) . |
| MLA | Wang, Lele et al. "Near-Infrared Light-Driven Photoredox Catalysis by Transition-Metal-Complex Nanodots" . | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 61 . 39 (2022) . |
| APA | Wang, Lele , Sa, Rongjian , Wei, Yingcong , Ma, Xiongfeng , Lu, Chenggang , Huang, Haowei et al. Near-Infrared Light-Driven Photoredox Catalysis by Transition-Metal-Complex Nanodots . | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION , 2022 , 61 (39) . |
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For laser-induced fluorescence (LIF), the SNR and related concentration detectability was severely limited by background signal (noise). Herein, an axial-excitation LIF based on pinhole metal-capillary (PMC) and direct laser-diode excitation was proposed. An overall similar to 480 fold improvement on SNR was realized, because the lasersidewall interaction (LSI) and related background signal (noise) can be effectively suppressed in PMC by avoidance of laser leakage and laser contact with capillary sidewall. For rhodamine B detection without sample enrichment, a detection limit of concentration (DLC) of 0.4 pM was obtained, which is 2.5 fold lower than the DLC (1 pM) of commercial LIFs equipped with bulky and expensive Ar+ laser. For selective detection of Cu2+, a DLC of 5 pM was realized, which is more than 40 fold lower than that of previous fluorimetry detection with similar quantum-dot probe. The PMC-LIF features compact (16 x 4 x 4 cm(3)), low-cost and high concentration detectability.
Keyword ：
Background signal Background signal Laser-induced fluorescence Laser-induced fluorescence Laser-sidewall interaction Laser-sidewall interaction Metal capillary Metal capillary Noise Noise
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| GB/T 7714 | Cai, Weicheng , Huang, Hui , Yang, Zhibo et al. A compact, low-cost and high sensitive LIF based on pinhole metal-capillary and direct laser-diode excitation [J]. | OPTICS AND LASERS IN ENGINEERING , 2021 , 139 . |
| MLA | Cai, Weicheng et al. "A compact, low-cost and high sensitive LIF based on pinhole metal-capillary and direct laser-diode excitation" . | OPTICS AND LASERS IN ENGINEERING 139 (2021) . |
| APA | Cai, Weicheng , Huang, Hui , Yang, Zhibo , Shang, Ruichen , Li, Xuejing , Ma, Chi et al. A compact, low-cost and high sensitive LIF based on pinhole metal-capillary and direct laser-diode excitation . | OPTICS AND LASERS IN ENGINEERING , 2021 , 139 . |
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