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学者姓名：李锋
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Abstract ：
In this work, the acute and subchronic toxicities of desaminotyrosine (DAT) by oral administration in SD rats and its effects on the intestinal microflora were investigated. The acute toxicity test showed that DAT is a low-toxic substance with a LD50 of 3129 mg/kg. The subchronic toxicity test showed that DAT has no toxicity at a low dose (125 mg/kg/day). However, DAT exhibited obvious toxicities to food intake, liver, kidney, and lung at higher dose (250 mg/kg/day and 500 mg/kg/day). DAT inhibited the food intake of rats in a dose-dependent manner. Serum biochemical analysis showed that DAT can increase the serum glucose level of rats. Fecal microbiota analysis showed that DAT treatment can significantly change the intestinal microflora of rats, the dose of 125 mg/kg/day has the most significant effect on the diversity of intestinal microbiota. In daily application, the side effects caused by DAT might be gastrointestinal irritation, weight loss, liver or kidney injury, and blood sugar elevation. Based on our study, the no-observed-adverse-effect level (NOAEL) of DAT is 125 mg/kg BW/day for rats.
Keyword ：
Acute toxicity Acute toxicity Desaminotyrosine Desaminotyrosine Intestinal microflora Intestinal microflora Metabolites of flavonoid Metabolites of flavonoid Subachronic toxicity Subachronic toxicity
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| GB/T 7714 | Li, Feng , Wang, Liping , Cai, Yilei et al. Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats [J]. | TOXICOLOGY AND APPLIED PHARMACOLOGY , 2021 , 417 . |
| MLA | Li, Feng et al. "Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats" . | TOXICOLOGY AND APPLIED PHARMACOLOGY 417 (2021) . |
| APA | Li, Feng , Wang, Liping , Cai, Yilei , Luo, Yihuo , Shi, Xianai . Safety assessment of desaminotyrosine: Acute, subchronic oral toxicity, and its effects on intestinal microbiota in rats . | TOXICOLOGY AND APPLIED PHARMACOLOGY , 2021 , 417 . |
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Traditional methods for aflatoxin B1 (AFB1) detection are complex, time-consuming, labor-intensive, and high cost. Moreover, they require sophisticated large-scale instrumentation, which limits their on-site rapid detection. Herein, phycocyanin fluorescent nanospheres based on fluorescence immunochromatographic assay were developed for quantitative detection of AFB1 at parts-per-billion (ppb) levels in foodstuffs. Phycocyanin and antiAFB1 monoclonal antibodies were coupled on the surface of latex nanospheres to amplify the fluorescence signal and improve the sensitivity. The fluorescence intensity was measured by a self-developed smartphone-based reading system. Under the optimal conditions, this approach achieved quantitative point-of-care detection of AFB1 within 25 min. The calibration curve for AFB1 was linear in the range of 0.2-48 ppb, and the limit of detection was 0.16 ppb. The practical applicability of the proposed approach was demonstrated by the determination of AFB1 in naturally contaminated samples, and the results were consistent with HPLC detection.
Keyword ：
Aflatoxin B1 Aflatoxin B1 Immunoassay Immunoassay On-site determination On-site determination Phycocyanin Phycocyanin Smartphone Smartphone
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| GB/T 7714 | Li, Shiqi , Zhong, Xiaoying , Xu, Yanan et al. Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs [J]. | FOOD CHEMISTRY , 2021 , 360 . |
| MLA | Li, Shiqi et al. "Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs" . | FOOD CHEMISTRY 360 (2021) . |
| APA | Li, Shiqi , Zhong, Xiaoying , Xu, Yanan , Zheng, Yunquan , Shi, Xianai , Li, Feng et al. Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs . | FOOD CHEMISTRY , 2021 , 360 . |
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将源自鲨鱼软骨的高纯度硫酸软骨素和胶原蛋白以质量比2∶1配伍,得到鲨鱼硫酸软骨素胶原蛋白复方(SCC),并考察了该复方对骨关节炎(OA)的改善作用。在实验的第0、2、4、6天向小鼠膝关节腔定量注射Ⅱ型胶原蛋白酶,构建骨关节炎小鼠实验模型。同时每天分别以300、150 mg·kg~(-1)剂量灌胃小鼠给予SCC干预,持续4周。之后,取关节组织,测定肿胀程度,病理切片染色分析软骨损伤情况;通过酶联免疫吸附法(ELISA)检测血清IL-1β和IL-4表达水平。关节病理切片H&E染色结果显示,SCC 300 mg·kg~(-1)剂量对软骨细胞的修复优于150 mg·kg~(-1)剂量;Masson和天...
Keyword ：
硫酸软骨素 硫酸软骨素 胶原蛋白 胶原蛋白 骨关节炎 骨关节炎 鲨鱼软骨 鲨鱼软骨
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| GB/T 7714 | 颜晨燕 , 王梨萍 , 屈鑫 et al. 鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究 [J]. | 海洋渔业 , 2020 , 42 (06) : 740-750 . |
| MLA | 颜晨燕 et al. "鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究" . | 海洋渔业 42 . 06 (2020) : 740-750 . |
| APA | 颜晨燕 , 王梨萍 , 屈鑫 , 李锋 , 石贤爱 . 鲨鱼硫酸软骨素及胶原蛋白复方改善小鼠骨关节炎作用研究 . | 海洋渔业 , 2020 , 42 (06) , 740-750 . |
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Acute full-thickness wounds require amore extended healing period, thus increasing the risk of infection. Severe infection frequently resulted in wound ulceration, necrosis, and even life-threatening complications. Here, a hybrid hydrogel comprising aminated collagen (AC), oxidized sodium alginate (OSA), and antimicrobial peptides (polymyxin B sulfate and bacitracin) was developed to enhance full-thickness wound healing. The AC with low immunogenicity and high biocompatibility was made from marine fish scales, which are eco-friendly, low-cost, and sustainable. The cross-linked hydrogel was formed by a Schiff base reaction without any catalysts and additional procedures. As expected, the presented hybrid hydrogel can effectively against E. coli and S. aureus, as well as promote cell growth and angiogenesis in vitro. In addition, the hydrogel can promote full-thickness wound healing in a rat model through accelerating reepithelialization, collagen deposition, and angiogenesis. Our work demonstrated that the hybrid hydrogel has promising applications in the field of wound healing, which would prompt the utilization of marine fish resources during food processing. (C) 2020 Elsevier B.V. All rights reserved.
Keyword ：
Antimicrobial peptide Antimicrobial peptide Fish scale Fish scale Hydrogel Hydrogel Sodium alginate Sodium alginate Wound dressing Wound dressing
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| GB/T 7714 | Feng, Xiaolian , Zhang, Xiaofang , Li, Shiqi et al. Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 164 : 626-637 . |
| MLA | Feng, Xiaolian et al. "Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 164 (2020) : 626-637 . |
| APA | Feng, Xiaolian , Zhang, Xiaofang , Li, Shiqi , Zheng, Yunquan , Shi, Xianai , Li, Feng et al. Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 164 , 626-637 . |
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Background: It has been demonstrated in some studies that triterpenoid acid extract fromEriobotrya japonica leaf is beneficial to prevent hyperlipidemia or insulin resistance. However, the effect of triterpenoid acids in Eriobotrya japonica leaf on a series of typical symptoms of metabolic syndrome (MetS) has been rarely studied systemati-cally. Therefore, the present study aims to systematically evaluate the effect of Eriobotrya japonica leaf triterpenoid acids (ELTA) on MetS and explore its potential mechanism. Methods: ELTA (HPLC purity 95.2 %) was prepared and administered orally (200 mg/kg) to C57BL/6 J mice fed with a high-fat diet (HFD) for 12 weeks. Pioglitazone (30 mg/kg) was used as a positive control drug. Food intake, body weight, total lipid in feces, lipid profiles, inflammatory factors in serum, hepatic glutathione, and lipid peroxide were measured. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed to evaluate insulin sensitivity. RT-qPCR and molecular docking were performed to explore the potential mechanism. Results: ELTA administration reduced body weight gain, relative liver weight, and relative visceral adipose weight. The levels of serum total cholesterol, triglycerides, low-density lipoprotein cholesterol, hepatic total cholesterol, and hepatic triglycerides were also reduced. ELTA reduced the area under curve (AUC) of blood glucose curves in OGTT and ITT. Relative mRNA level analysis of genes related to MetS showed that ELTA can effectively increase the transcriptional levels of Nrf2, HO-1, PPAR-gamma, GluT2, GK, FXR, while effectively decrease those of PTP1B, p65, TNF-alpha, IL-6, SREBP, 11 beta HSD-1. Molecular docking showed that the ligands in ELTA can bind to 11 beta HSD-1, GK, PPAR-gamma, and JNK, the important targets involved in MetS. Conclusions: ELTA can effectively alleviate visceral central obesity, insulin resistance, dyslipidemia, oxidative stress, and inflammation of HFD-induced MetS in C57BL/6 J mice. This is possibly achieved by acting on 11 beta HSD-1, GK, PPAR-gamma, and JNK.
Keyword ：
Dyslipidemia Dyslipidemia Eriobotrya japonica leaf triterpenoid acids Eriobotrya japonica leaf triterpenoid acids High-fat diet High-fat diet Insulin resistance Insulin resistance Metabolic syndrome Metabolic syndrome Molecular docking Molecular docking
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| GB/T 7714 | Li, Feng , Li, Yijia , Li, Qingxian et al. Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet [J]. | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 132 . |
| MLA | Li, Feng et al. "Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet" . | BIOMEDICINE & PHARMACOTHERAPY 132 (2020) . |
| APA | Li, Feng , Li, Yijia , Li, Qingxian , Shi, Xianai . Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet . | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 132 . |
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Wound dressings with long-term antimicrobial activity are highly desired for treatment of chronic wound infections. Herein, the sustained antimicrobial wound dressings were developed by using antibiotic agents, ciprofloxacin HCL (CIP) and gentamicin sulfate (GS), covalent bonding to natural polymer matrix composites, carboxymethyl chitosan (CMC) and collagen (COL). By amide bond formation between antibiotic agents and polymer chains, two antimicrobial wound dressings CMC-COL-CIP and CMC-COL-GS were prepared. The presented wound dressings exhibited high water absorption capacity, excellent water vapor transmission rate (WVTR), appropriate mechanical properties, and impressive stability. Cytocompatibility of the dressings was demonstrated by in vitro human skin fibroblast (HSF) cells culture study. The results of in vitro and in vivo studies indicated that the two antimicrobial wound dressings have effective antimicrobial activity and prolonged antimicrobial period. Furthermore, the antimicrobial dressings could promote the wound healing, reepithelialization, collagen deposition, and angiogenesis. It also displays superiority wound healing effects compared to commercially available silver-based dressings (Aguacel Ag). This work indicates that the prepared antimicrobial wound dressings have great potential application in chronic wound healing, such as severe wound cure and diabetic foot ulcers. (C) 2018 Elsevier B.V. All rights reserved.
Keyword ：
Antimicrobial activity Antimicrobial activity Carboxymethyl chitosan Carboxymethyl chitosan Collagen Collagen Wound dressings Wound dressings Wound infections Wound infections
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| GB/T 7714 | Hu, Shengxue , Cai, Xianqun , Qu, Xin et al. Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2019 , 123 : 1320-1330 . |
| MLA | Hu, Shengxue et al. "Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 123 (2019) : 1320-1330 . |
| APA | Hu, Shengxue , Cai, Xianqun , Qu, Xin , Yu, Bangrui , Yan, Chenyan , Yang, Jianmin et al. Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2019 , 123 , 1320-1330 . |
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Herein, an autonomous table-tennis-motion-type DNA machine (ATT DNA machine) was proposed to integrate target recycling amplification (TRA) and triple strand-displacement amplification (T-SDA) into one signaling system for the efficient amplification detection of p53 gene. Firstly, X-shaped DNA duplex probe (XSP) was formed via the simple annealing of Probe A and Probe B. Upon addition of p53, the hybridization of p53 with Probe A results in the peeling of Probe B from XSP. Then, the primer binds to the Probe B to trigger a circular strand-displacement amplification, during which new primers are generated. Moreover, the newly-produced primers can in turn activate the target recycling amplification and triple strand-displacement amplification via bouncing back and forth between different Probe As and Probe Bs in a manner analogous to the table tennis motion. This newly-developed DNA machine can be used to detect p53 down to 0.5 pM with an excellent specificity, offering a 100-fold enhanced sensitivity compared to a non-amplification one-to-one hybridization based system. Moreover, this DNA machine is able to be applied for target analysis in complex environments. Additionally, the potential for practical applications is also verified, demonstrating a promising alternative tool for early diagnosis of cancers.
Keyword ：
ATT DNA machine ATT DNA machine p53 gene p53 gene Target recycling amplification (TRA) Target recycling amplification (TRA) Triple strand-displacement amplification (TSDA) Triple strand-displacement amplification (TSDA) X-shaped DNA duplex probe (XSP) X-shaped DNA duplex probe (XSP)
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| GB/T 7714 | Xu, Huo , Zhang, Yafeng , Zhang, Shuxin et al. Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2019 , 281 : 1016-1022 . |
| MLA | Xu, Huo et al. "Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene" . | SENSORS AND ACTUATORS B-CHEMICAL 281 (2019) : 1016-1022 . |
| APA | Xu, Huo , Zhang, Yafeng , Zhang, Shuxin , Ouyang, Changhe , Li, Feng , Yu, Suhong et al. Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene . | SENSORS AND ACTUATORS B-CHEMICAL , 2019 , 281 , 1016-1022 . |
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In the human genome, single-nucleotide polymorphisms (SNPs) can often lead to the activation of oncogenes and have a close association with tumor development or progression. Specific identification of SNPs and quantitative analysis of oncogenes could provide insight into the basis for early diagnosis and risk assessment of malignancy, as well as personalized medicine. Herein, we proposed a new biosensing platform to screen SNPs through the ligation/polymerization-mediated signaling of a split molecular beacon (SMB) where target-recycling benefits can be achieved. The SMB consists of two fragments, one of which contains a fluorophore group and the other was modified with a quencher group. In the absence of target DNA, the two fragments hybridize with each other and the fluorescence is quenched. After exposure to a wild-type target, they can be opened and ligated together by the ligase using the target as the template. Moreover, the primer is able to bind to target hybridization-released binding sites and initiate strand-displacement amplification (SDA), generating an enhanced signal. By combining the specificity of nick ligation with the signal amplification of target recycling, the human K-ras oncogene as a model target can be detected down to 8 pM. Moreover, a wide dynamic range (more than 3 orders of magnitude) and excellent ability to discriminate single-base mutations are achieved. Due to the homogeneous reaction and simple experimental steps, the target assay is greatly cost-efficient and readily automated. The desirable features and advantages make the proposed genotyping strategy a promising platform for genetic analysis and molecular diagnostics.
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| GB/T 7714 | Li, Min , Jiang, Min , Yang, Shu-Lin et al. A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism [J]. | ANALYTICAL METHODS , 2019 , 11 (25) : 3252-3259 . |
| MLA | Li, Min et al. "A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism" . | ANALYTICAL METHODS 11 . 25 (2019) : 3252-3259 . |
| APA | Li, Min , Jiang, Min , Yang, Shu-Lin , Guo, Fei-Xia , Wu, Bi-Ting , Zhang, Meng-Yi et al. A split molecular beacon for specific identification of cancer-related single nucleotide polymorphism . | ANALYTICAL METHODS , 2019 , 11 (25) , 3252-3259 . |
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The purpose of the current study was to construct a -cyclodextrin drug carrier for rubropunctatin to improve its water solubility and light stability for future cytotoxicity studies. The inclusion complexation behavior of rubropunctatin with -cyclodextrin was investigated using FESEM, FT-IR and XRD. A molecular docking study was performed to elucidate the most probable inclusion structure. The inclusion complex could be completely dispersed in water and had a small size of 121.87 +/- 2.13 nm (n = 3), a good PDI (0.320 +/- 0.017), and an acceptable potential value of -27.7 +/- 0.32 mV (n = 3). Furthermore, the stability of the rubropunctatin in water under light irradiation was found to be greatly enhanced after being encapsulated in cyclodextrin, and it exhibited a retention rate of over 70% vs. 10.17%. In addition, the cytotoxicity of the inclusion complex was evaluated by MTT assay and Annexin V-FITC/PI detection using cervical adenocarcinoma HeLa cells. The results showed that the inclusion complex had comparable toxicity compared to rubropunctatin solubilized with 0.4% DMSO. More importantly, the formation of the inclusion complex contributed greatly to the intensification of the bioavailability of rubropunctatin because the use of organic solvent was avoided.
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| GB/T 7714 | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier [J]. | RSC ADVANCES , 2019 , 9 (20) : 11396-11405 . |
| MLA | Ren, Zhenzhen et al. "Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier" . | RSC ADVANCES 9 . 20 (2019) : 11396-11405 . |
| APA | Ren, Zhenzhen , Xu, Yanan , Lu, Zhenxin , Wang, Zhenzhen , Chen, Chengqun , Guo, Yanghao et al. Construction of a water-soluble and photostable rubropunctatin/-cyclodextrin drug carrier . | RSC ADVANCES , 2019 , 9 (20) , 11396-11405 . |
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Phycocyanin (PC) is a natural fluorescent protein which is widely used in medical diagnosis, biology, biotechnology, foods, and medicine. However, the disadvantages in traditional purification methods, such as complicated preparation process, low yield, inadequate quantity, and high price, limit its range of application. In this study, we established a rapid purification method suitable for large-scale production to prepare PC from Arthrospira platensis. The membrane separation and two-stage ammonium sulfate precipitation yielded PC with a purity ratio (A(620nm)/A(280nm)) of 3.65. This purification method of PC proved to be efficient and cost-effective compared with previously reported methods. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the molecular weight of alpha and beta subunits of PC was 16.6 and 21.2 kDa. The main molecular weight of intact PC determined through high-performance size exclusion chromatography (HPSEC) was around 91 kDa, indicating that the PC from A. platensis existed mainly in the form of (alpha beta)(3) at pH 7.0. The purified PC fluorescent probe was compatible for light-emitting diode (LED)-charge-coupled device (CCD) fluorescence density strip qualitative detection system. A relatively sensitive novel fluorescent test method was developed for the quantitative detection of PC showing a linear response to PC within the range of 0.07-3.64 mu M, and with a limit of detection of 0.034 mu M. This method could provide expedient quantitative information and showed great potential for rapid detection in environmental and food safety research.
Keyword ：
Fluorescent protein Fluorescent protein Large-scale purification Large-scale purification Rapid detection Rapid detection
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| GB/T 7714 | Zheng, Yunquan , Mo, Liuda , Zhang, Wenyi et al. Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system [J]. | JOURNAL OF APPLIED PHYCOLOGY , 2019 , 31 (2) : 1107-1115 . |
| MLA | Zheng, Yunquan et al. "Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system" . | JOURNAL OF APPLIED PHYCOLOGY 31 . 2 (2019) : 1107-1115 . |
| APA | Zheng, Yunquan , Mo, Liuda , Zhang, Wenyi , Duan, Yunjian , Huang, Jiandong , Chen, Chengqun et al. Phycocyanin fluorescent probe from Arthrospira platensis: preparation and application in LED-CCD fluorescence density strip qualitative detection system . | JOURNAL OF APPLIED PHYCOLOGY , 2019 , 31 (2) , 1107-1115 . |
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