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学者姓名：王航
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目的:观察可溶性糖蛋白130(sgp130)对糖尿病(DM)小鼠视网膜p-STAT3及VEGF-A表达的影响,探讨sgp130防治糖尿病视网膜病变(DR)炎性损害的可能性.方法:小鼠45只被随机分为正常组、DM组和sgp130组.采用链脲佐菌素对DM组和sgp130组进行DM造模.正常组、DM组不做特殊干预,sgp130组在第1、5wk被给予1.5mg/mL sgp1302μL进行玻璃体腔注射治疗.10wk后处死所有小鼠,检查视网膜组织IL-6、p-STAT3、VEGF-A等蛋白的表达.结果:在第10wk时,DM组视网膜IL-6、p-STAT3、VEGF-A表达较正常组升高(均P<0.01).sgp130组p-STAT3、VEGF-A表达水平较DM组低(均P<0.01).结论:sgp130可以选择性拮抗IL-6反式信号传导通路,下调下游炎性因子VEGF-A的表达,可以用于干预DM引起的IL-6相关性视网膜炎性损害.
Keyword ：
p-STAT3 p-STAT3 可溶性糖蛋白130(sgp130) 可溶性糖蛋白130(sgp130) 炎症 炎症 白介素6 白介素6 糖尿病视网膜病变 糖尿病视网膜病变 血管内皮生长因子A(VEGF-A) 血管内皮生长因子A(VEGF-A)
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| GB/T 7714 | 刘光辉 , 史常旋 , 洪雅军 et al. sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 [J]. | 国际眼科杂志 , 2023 , 23 (3) : 375-378 . |
| MLA | 刘光辉 et al. "sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响" . | 国际眼科杂志 23 . 3 (2023) : 375-378 . |
| APA | 刘光辉 , 史常旋 , 洪雅军 , 郑永征 , 王航 , 孟春 . sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 . | 国际眼科杂志 , 2023 , 23 (3) , 375-378 . |
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应用酶反应动力学原理对海藻酸钠裂解酶酶活测定反应体系和反应条件进行系统研究,以改进海藻酸钠裂解酶的测定方法.本实验采用紫外吸收法测定酶活,改良后的酶活测定方法为:300 μL底物溶液(海藻酸钠2.2％,KCl 5 mmol/L,0.1 mol/L Na2HPO4-NaH2PO4缓冲液,pH7.0)加入50 μL 稀释至2.4～30 U/mL 的酶液,于37℃水浴静置反应20 min后用冰浴终止反应,反应液稀释20倍后在235 nm处测定吸光度.每份底物溶液均需用新枪头移取以提高精密度,使移液误差小于2％.本酶活测定方法的相对标准偏差小于5％.
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| GB/T 7714 | 郑明亮 , 郑诨龙 , 孟春 et al. 海藻酸钠裂解酶酶活测定方法研究 [J]. | 食品工业科技 , 2021 , 42 (7) : 246-251 . |
| MLA | 郑明亮 et al. "海藻酸钠裂解酶酶活测定方法研究" . | 食品工业科技 42 . 7 (2021) : 246-251 . |
| APA | 郑明亮 , 郑诨龙 , 孟春 , 王航 . 海藻酸钠裂解酶酶活测定方法研究 . | 食品工业科技 , 2021 , 42 (7) , 246-251 . |
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新型冠状病毒(severe acute respiratory syndrome coronavirus-2, SARS-CoV-2)是一种可引起人新型冠状病毒肺炎(novel coronavirus pneumonia, NCP;亦称为COVID-19)的新发呼吸道病原体,与中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus, MERS-CoV)和严重急性呼吸综合征冠状病毒(severe acute respiratory syndrome coronavirus, SARS-CoV)同属β-冠状病毒,其受体与SARS-CoV的受...
Keyword ：
新型冠状病毒 新型冠状病毒 疫苗 疫苗 肺炎 肺炎 血管紧张素转化酶2 血管紧张素转化酶2
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| GB/T 7714 | 梁育玮 , 赖玲玲 , 王航 . 新型冠状病毒致病机理及其疫苗的研发进展 [J]. | 微生物学免疫学进展 , 2020 , 48 (04) : 68-73 . |
| MLA | 梁育玮 et al. "新型冠状病毒致病机理及其疫苗的研发进展" . | 微生物学免疫学进展 48 . 04 (2020) : 68-73 . |
| APA | 梁育玮 , 赖玲玲 , 王航 . 新型冠状病毒致病机理及其疫苗的研发进展 . | 微生物学免疫学进展 , 2020 , 48 (04) , 68-73 . |
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A real-time electric nose (E-nose) with a metal oxide sensor (MOS) array was developed to monitor 5 highly flammable liquids (ethanol, tetrahydrofuran, turpentine, lacquer thinner, and gasoline) in this work. We found that temperature had a significant impact on the test results and temperature control could efficiently improve the performance of our E-nose. The results of our qualitative analysis showed that principal component analysis (PCA) could not efficiently distinguish these samples compared to a back-propagation artificial neural network (BP-ANN) which had a 100% accuracy rate on the test samples. Quantitative analysis was performed by regression analysis and the average errors were 9.1%-18.4%. In addition, through anti-interference training, the E-nose could filter out the potential false alarm caused by mosquito repellent, perfume and hair jelly.
Keyword ：
artificial neural network artificial neural network back propagation back propagation electronic nose electronic nose flammable liquids flammable liquids qualitative and quantitative analysis qualitative and quantitative analysis
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| GB/T 7714 | Wu, Zhiyuan , Wang, Hang , Wang, Xiping et al. Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids [J]. | SENSORS , 2020 , 20 (7) . |
| MLA | Wu, Zhiyuan et al. "Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids" . | SENSORS 20 . 7 (2020) . |
| APA | Wu, Zhiyuan , Wang, Hang , Wang, Xiping , Zheng, Hunlong , Chen, Zhiming , Meng, Chun . Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids . | SENSORS , 2020 , 20 (7) . |
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目的 建立一种简便的大鼠主动脉血管内皮细胞(vascular endothelial cells,VECs)分离纯化及培养方法.方法 无菌条件下分离获得大鼠主动脉,在眼科显微器械的辅助下翻转暴露血管内膜.按处理方式的不同,将获得的主动脉血管段分为消化压片法组、单纯消化法组和单纯压片法组,并进行对应的处理和培养,通过倒置相差显微镜观察VECs形态,免疫荧光法鉴定VECs的细胞标记物.结果 消化压片法组在接种后48～72 h即有VECs自血管段中迁移出,原代细胞约10～11 d可达到近融合状态,子代细胞生长速度加快,5 d后达到融合状态,呈典型的"铺路石"样特征.相对于其他两组,消化压片法组VECs的迁移速度快、细胞数量多.免疫学鉴定显示细胞标志物Ⅷ因子、vWF和CD31的阳性率高达99％.所培养的VECs传代培养至第10代,未见明显的细胞形态特征变化,免疫荧光鉴定至第8代,未见明显的细胞免疫特征变化.结论 本研究成功建立了一种简单经济的大鼠主动脉VECs分离、纯化和培养方法.
Keyword ：
主动脉 主动脉 分离 分离 大鼠 大鼠 纯化 纯化 细胞培养 细胞培养 血管内皮细胞 血管内皮细胞
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| GB/T 7714 | 林翠红 , 刘光辉 , 杨田野 et al. 大鼠主动脉内皮细胞改良型分离培养方法 [J]. | 西安交通大学学报：医学版 , 2018 , 39 (6) : 911-916 . |
| MLA | 林翠红 et al. "大鼠主动脉内皮细胞改良型分离培养方法" . | 西安交通大学学报：医学版 39 . 6 (2018) : 911-916 . |
| APA | 林翠红 , 刘光辉 , 杨田野 , 赵利 , 王航 , 史常旋 et al. 大鼠主动脉内皮细胞改良型分离培养方法 . | 西安交通大学学报：医学版 , 2018 , 39 (6) , 911-916 . |
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为开展半滑舌鳎(Cynoglossus semilaevis)免疫学研究提供细胞平台, 利用密度梯度离心法分离半滑舌鳎外周血淋巴细胞, 采用短期细胞培养法分离悬浮淋巴细胞, 悬浮淋巴细胞在含有0.3 μg/mL的PHA的DMEM完全培养基, 于24℃条件下可连续培养3—4d左右, 采用自制的尼龙毛柱可将悬浮淋巴细胞中的非黏附淋巴细胞和黏附淋巴细胞成功分离; 利用流式细胞仪结合特异抗体检测对非黏附淋巴细胞和黏附淋巴细胞进行鉴定,结果表明, 非黏附细胞与鼠抗人FTIC-CD3单克隆抗体特异结合, 为T样淋巴细胞; 黏附细胞和鼠抗人FTIC-CD19单抗特异结合,为B样淋巴细胞.T细胞表面抗原受体TCRβ基因可特异性的在非黏膜细胞中表达,而在黏附细胞中不表达, 证明分离获得的非黏膜细胞为T淋巴细胞, 采用qRT-PCR (Quantitative Real-Time PCR)方法检测TCRβ基因表达, 结果表明, TCRβ基因在半滑舌鳎肝、脾、头肾、后肾、小肠、胃、血液、鳃、皮肤、肌肉、心脏、脑、卵巢组织中均有表达, 其中在肠、胃、脾、头肾中表达量较高; 鳗弧菌感染后TCRβ基因在肝、脾、鳃中呈现明显的上调表达,且表达峰值出现在感染后72—96h,表明TCRβ基因在获得性免疫应答中起重要作用.
Keyword ：
B淋巴细胞 B淋巴细胞 TCRβ基因 TCRβ基因 T淋巴细胞 T淋巴细胞 免疫应答 免疫应答 半滑舌鳎 半滑舌鳎 外周血 外周血
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| GB/T 7714 | 汪林庆 , 王航 , 陈亚东 et al. 半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析 [J]. | 水生生物学报 , 2018 , 42 (3) : 542-549 . |
| MLA | 汪林庆 et al. "半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析" . | 水生生物学报 42 . 3 (2018) : 542-549 . |
| APA | 汪林庆 , 王航 , 陈亚东 , 杨光 , 白莉 , 孙璐明 et al. 半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析 . | 水生生物学报 , 2018 , 42 (3) , 542-549 . |
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A limited number of xenobiotic-induced inflammatory episodes occur in the human liver and small intestine under normal physiological conditions, although many xenobiotic agents are metabolized in these organs every day. In this study, we found that rifampicin-activated pregnane X receptor (PXR) plays an important role in the suppression of lipopolysaccharide-activated nuclear factor kappa B (NF-kappa B) activity by increasing the expression of the inhibitor of kappa B alpha (I kappa B alpha) in HepG2 cells. Rifampicin did not increase the expression of I kappa B alpha in PXR knockdown HepG2 cells. Furthermore, we found that the activation of PXR could inhibit the lipopolysaccharide-stimulated nuclear translocation of NF-kappa B p50/p65 using electrophoretic mobility shift assay, immunoprecipitation assays, and microscopy. High levels of I kappa B alpha directly interacted with NF-kappa B and prevented its nuclear translocation, thus inhibiting its binding to consensus DNA sequences in nuclei. Xenobiotic-activated tissue-specific PXR might exert anti-inflammatory actions by antagonizing the xenobiotic-induced transcriptional activity of NF-kappa B in the liver and small intestine. The results also showed that activation of PXR arrested the HepG2 cell cycle in the G(0)/G(1) phase and exhibited cancer prevention potential by inhibiting inflammatory reactions in cells.
Keyword ：
Anti-inflammation Anti-inflammation Cancer prevention Cancer prevention Inhibitor of kappa B alpha Inhibitor of kappa B alpha Pregnane X receptor Pregnane X receptor
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| GB/T 7714 | Ye, Nanhui , Wang, Hang , Li, Qiaoling et al. Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells [J]. | MOLECULAR & CELLULAR TOXICOLOGY , 2018 , 14 (1) : 93-104 . |
| MLA | Ye, Nanhui et al. "Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells" . | MOLECULAR & CELLULAR TOXICOLOGY 14 . 1 (2018) : 93-104 . |
| APA | Ye, Nanhui , Wang, Hang , Li, Qiaoling , Lin, Chaotong , Feng, Huahua , Lin, Suying et al. Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells . | MOLECULAR & CELLULAR TOXICOLOGY , 2018 , 14 (1) , 93-104 . |
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本发明公开了阵列式加样器，其包括若干个加样针、若干个储液模块、若干个输送驱动装置和气源，若干个储液模块与若干个加样针一一对应且连接，若干个输送驱动装置的一端均与气源连接，若干个输送驱动装置的另一端与若干个储液模块一一对应且连接并用于将储液模块中的试剂推送至加样针进行输出加样，其实现了不同试剂使用不同的试剂针进行加样，免去了试剂针清洗的过程，减少了加样所需时间并简化了运动结构，从而可以使每次加样所需时间减少，保证了在多种不同第一抗体试剂滴加过程中不同切片接触试剂时间的差距大大减小，保证了染色结果的一致性与可重复性，在仪器运行过程中所存储的试剂与空气的接触面积极小，保证试剂的安全性和纯净性。
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| GB/T 7714 | 孟春 , 钟乐 , 王航 . 阵列式加样器 : CN201810023012.4[P]. | 2018/1/10 . |
| MLA | 孟春 et al. "阵列式加样器" : CN201810023012.4. | 2018/1/10 . |
| APA | 孟春 , 钟乐 , 王航 . 阵列式加样器 : CN201810023012.4. | 2018/1/10 . |
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The Escherichia coli aspartate aminotransferase gene was introduced into a high 2-phenylethanol (2-PEA) producing Saccharomyces cerevisiae YS58, and the recombinant strain of S. cerevisiae was utilized for the co-production of 2-PEA and L-homophenylalanine (L-HPA) via a fermentation process. The L-HPA productivity of the recombinant S. cerevisiae improved 78.9% in comparison to the wild-type S. cerevisiae. High yields of 43.7 mM L-HPA and 32.4 mM 2-PEA were achieved. As a result, the coupling of the biosynthesis process for these two products in the recombinant strain led to a more complete and efficient utilization of the substrate, L-phenylalanine. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
Keyword ：
2-Phenylethanol 2-Phenylethanol Aspartate aminotransferase Aspartate aminotransferase Cosynthesis Cosynthesis L-Homophenylalanine L-Homophenylalanine Saccharomyces cerevisiae Saccharomyces cerevisiae
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| GB/T 7714 | Luo, Chunhua , Lin, Qingmei , Lin, Suying et al. Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3) [J]. | JOURNAL OF BIOSCIENCE AND BIOENGINEERING , 2017 , 123 (1) : 1-7 . |
| MLA | Luo, Chunhua et al. "Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3)" . | JOURNAL OF BIOSCIENCE AND BIOENGINEERING 123 . 1 (2017) : 1-7 . |
| APA | Luo, Chunhua , Lin, Qingmei , Lin, Suying , Meng, Chun , Wang, Hang . Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3) . | JOURNAL OF BIOSCIENCE AND BIOENGINEERING , 2017 , 123 (1) , 1-7 . |
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OBJECTIVE: To construct a mouse single-chain fragment variable (scFv) antibody library specific to human P53 by overlapping PCR method and screen the single chain antibodies against human P53 protein with the yeast two-hybrid (Y2H) system.METHODS: The bait vector pGBKT-p53 expressing P53 protein in yeast AH109 cells was constructed by means of genetic engineering method. The total RNA which was extracted from the P53-immunized mouse spleen tissue was used to synthesize the single chain V(H)-linker-V(L) fragment by reverse transcription-PCR and overlapping PCR. And then the V(H)-linker-V(L) fragment constructed on the vector pGADT7 was transformed into the AH109 cells containing the bait vector. The positive clones were screened on the nutrition auxotrophic media. The characteristics of scFv were verified by immunocytochemistry.RESULTS: The bait vector pGBKT7-p53 was constructed successfully and transformed into AH109 cells. It had no self-activation in the yeast cells and no toxicity to the host. The library of V(H)-linker-V(L) was successfully obtained. The captured vector harboring V(H)-linker-V(L) fragments was constructed. We screened out the human P53 scFvs by the Y2H system. And scFv1/2/3 was proved to have a good affinity for human P53 protein, so it could be used for the identification of P53 protein in cells and tissues.CONCLUSION: We obtained human P53 scFvs with a good affinity for human P53 protein by Y2H system, which provided a new way for screening scFv.
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| GB/T 7714 | Zheng, M. , Li, M. , Shen, G. et al. Screening of special scFv antibody against human p53 protein by yeast two-hybrid system [J]. | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology , 2016 , 32 (1) : 112-117 . |
| MLA | Zheng, M. et al. "Screening of special scFv antibody against human p53 protein by yeast two-hybrid system" . | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 32 . 1 (2016) : 112-117 . |
| APA | Zheng, M. , Li, M. , Shen, G. , Lin, S. , Li, H. , Meng, C. et al. Screening of special scFv antibody against human p53 protein by yeast two-hybrid system . | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology , 2016 , 32 (1) , 112-117 . |
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