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学者姓名:邱彬
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A highly efficient and sensitive non-enzymatic glucose sensor fabricated using NiO/NiPc composite nanomaterials was introduced by this study. The sensor employed a unique design by modifying the ITO electrode with the NiO/NiPc heterojunction. This enhancement was achieved by improving charge separation and transfer through the type-II heterojunction formed between NiO and NiPc. The integration of NiO/NiPc composite materials also improved the electrocatalytic activity. This enhancement was attributed to the increased concentration of oxygen vacancies, thereby boosting the overall performance of the sensor. The results of the sensor application demonstrated that under the optimized conditions (pH 4.0, -0.3 V bias), the sensor exhibited remarkable sensitivity and selectivity towards glucose, with a linear relationship between the photocurrent change and glucose concentration. The detection limit was as low as 0.016 nM, and the sensitivity was 4.146 mu A & sdot;mM- 1 & sdot;cm- 2. The sensor demonstrated excellent repeatability, stability, and high recovery rate in real sweat sample testing, highlighting its great potential for practical glucose detection applications.
Keyword :
Glucose Glucose NiO NiO NiPc NiPc Photoelectrochemical sensor Photoelectrochemical sensor
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GB/T 7714 | Xue, Xiaoyan , Wu, Wenru , Yang, Yating et al. Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing [J]. | MICROCHEMICAL JOURNAL , 2025 , 208 . |
MLA | Xue, Xiaoyan et al. "Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing" . | MICROCHEMICAL JOURNAL 208 (2025) . |
APA | Xue, Xiaoyan , Wu, Wenru , Yang, Yating , Lin, Yue , Luo, Fang , Lin, Cuiying et al. Oxygen vacancy-enriched NiO/NiPc heterojunction for highly efficient and sensitive non-enzymatic glucose sensing . | MICROCHEMICAL JOURNAL , 2025 , 208 . |
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The overexpression of microRNA-21 (miRNA-21) is closely associated with gastric cancer (GC) and may be used as a biomarker to diagnose GC early. Here, a colorimetry and fluorescence dual-mode biosensor for the detection of miRNA-21 was developed from polydopamine-coated magnetic upconversion nanoparticles (Fe3O4@UCNP@PDA) and aptamer-modified gold nanoparticles (AuNP-apt). The strong adsorption of singlestranded DNA by PDA results in close proximity between the energy donors (UCNPs) and acceptors (AuNPs), resulting in luminescence quenching of the UCNPs. The miRNA-21 can hybridize with the apt-conjugated AuNPs, and the AuNPs move away from the UCNPs, which results in the luminescence recovery of the UCNPs and the catalytic activity of the AuNPs. After H2O2 and tetrapaminophenylethylene were added, the yellow solution turned green and gradually deepened with increasing miRNA-21 concentration. Under optimal conditions, the detection limits of miRNA-21 by fluorescence and colorimetry assays are 8.90 pM and 185 pM, respectively. In addition, the developed approach was utilized to measure miRNA-21 in serum samples, and the recovery rates ranged from 105.4-107.1% and 98.5-109.3%, respectively. The above results indicate great promise for the early diagnosis of GC because of its high sensitivity and selectivity.
Keyword :
Dual-mode detection Dual-mode detection Magnetic upconversion nanoparticle Magnetic upconversion nanoparticle microRNA-21 microRNA-21 Polydopamine Polydopamine Serum Serum
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GB/T 7714 | Chen, Wu , Guo, Mengfan , Chen, Zeyu et al. A fluorescence and colorimetric dual-mode biosensor based on the catalytic activity of gold nanoparticles and magnetic upconversion nanoparticles for the detection of microRNAs [J]. | MICROCHEMICAL JOURNAL , 2025 , 214 . |
MLA | Chen, Wu et al. "A fluorescence and colorimetric dual-mode biosensor based on the catalytic activity of gold nanoparticles and magnetic upconversion nanoparticles for the detection of microRNAs" . | MICROCHEMICAL JOURNAL 214 (2025) . |
APA | Chen, Wu , Guo, Mengfan , Chen, Zeyu , Sun, Ying , Huang, Yudian , Chen, Yushan et al. A fluorescence and colorimetric dual-mode biosensor based on the catalytic activity of gold nanoparticles and magnetic upconversion nanoparticles for the detection of microRNAs . | MICROCHEMICAL JOURNAL , 2025 , 214 . |
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The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword :
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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As a typical proinflammatory cytokine, interleukin 6 (IL-6) exhibits great influence on treating diseases (e.g., urocystitis) and adjusting immune. Normally, the IL-6 degree keeps a low content (< 40.0 pg mL(-1)) in bodies, but when its degree becomes higher, several serious conditions (e.g., urocystitis and infections) may occur. Hence, it is very essential to design a highly effective technology for monitoring IL-6 as a biomarker. In the present work, through adopting carbon black (CB) as substrate substance, o-diaminobenzene as electropolymerized monomer and [Fe(CN)(6)](4-/3-) as the signal probe, a low-cost, sensitive and simple molecularly imprinted polymer (MIP) electrochemical sensing platform was designed to detect the IL-6 degree. The experiments revealed that the current of [Fe(CN)(6)](4-/3- )probe at the MIP sensor would decrease along with the increase of IL-6 degree. Owing to the prominent properties of CB (e.g., high conductivity and surface area as well as stability) and high target-recognition capability of MIP, the as-proposed MIP sensor offers wide linearity (0.1-800 pg mL(-1)) and low analytical limit (0.03 pg mL(-1)); meanwhile, it possesses good repeatability, stability, and practical applications. It is, thus, expected this simple MIP sensor has some application value for IL-6 detection.
Keyword :
Carbon black Carbon black Electrochemical sensor Electrochemical sensor Interleukin 6 Interleukin 6 Molecular imprinting Molecular imprinting
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GB/T 7714 | Cui, Xu , Chen, Zhiqiang , Xu, Longyao et al. Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6 [J]. | ANALYTICAL SCIENCES , 2025 , 41 (4) : 487-494 . |
MLA | Cui, Xu et al. "Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6" . | ANALYTICAL SCIENCES 41 . 4 (2025) : 487-494 . |
APA | Cui, Xu , Chen, Zhiqiang , Xu, Longyao , Wu, Changwei , Qiu, Bin , Zhou, Chaoming . Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6 . | ANALYTICAL SCIENCES , 2025 , 41 (4) , 487-494 . |
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Real time detection of hydrogen sulfide (H2S) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (Cu2O NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. Cu2O reacts with H2S to generate Cu2S. The resulting Cu2O/Cu2S heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of H2S concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive in vivo biomarker detection.
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GB/T 7714 | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7467-7475 . |
MLA | Zeng, Yulan et al. "Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7467-7475 . |
APA | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui , Luo, Fang , Qiu, Bin , Fu, Fengfu et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7467-7475 . |
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Interleukin-6 (IL-6) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases including nasopharynx cancer, pancreatitis, infections, and cardiovascular. It is thus necessary to design an effective method for monitoring IL-6. Herein, by preparing gold nanoparticles/graphdiyne (Au/GRD) nanohybrid with simple electroless deposition as the sensing substrate and UiO-66 metal-organic frameworks/ferrocene (UiO/Fc) as the signaling-amplifier, a highly efficient sandwich-type electrochemical immunosensing (SEI) platform was proposed for IL-6 determination. The superior electrical properties, easy preparation, high surface area, and stability of Au/GRD make it a desirable substrate to immobilize the primary antibody of IL-6. Meanwhile, the unique structure of UiO-66 is beneficial to immobilizing the Fc probe and the secondary antibody. By optimizing experimental conditions, the Fc current obtained at the as-designed SEI platform increases along with IL-6 increase, and the related linearity is 0.5 pg ml-1-1200.0 pg ml-1 coupled with a low detection limit of 0.2 pg ml-1. It is expected the as-designed SEI platform based on Au/GRD and UiO/Fc shows potential applications for IL-6 analysis.
Keyword :
electrochemical sensor electrochemical sensor graphdiyne graphdiyne immunosensor immunosensor interleukin-6 interleukin-6 nasopharynx cancer nasopharynx cancer UiO-66 UiO-66
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GB/T 7714 | Wang, Xiaoyan , Zheng, Hao , Chen, Huiling et al. Gold Nanoparticles/Graphdiyne as Sensing Substrate Coupled with UiO-66/ferrocene as Signaling-Amplifier for Sandwich-Type Electrochemical Immunosensing of Interleukin-6 [J]. | JOURNAL OF THE ELECTROCHEMICAL SOCIETY , 2025 , 172 (3) . |
MLA | Wang, Xiaoyan et al. "Gold Nanoparticles/Graphdiyne as Sensing Substrate Coupled with UiO-66/ferrocene as Signaling-Amplifier for Sandwich-Type Electrochemical Immunosensing of Interleukin-6" . | JOURNAL OF THE ELECTROCHEMICAL SOCIETY 172 . 3 (2025) . |
APA | Wang, Xiaoyan , Zheng, Hao , Chen, Huiling , Qiu, Bin , Liu, Yunliang , Ye, Qing . Gold Nanoparticles/Graphdiyne as Sensing Substrate Coupled with UiO-66/ferrocene as Signaling-Amplifier for Sandwich-Type Electrochemical Immunosensing of Interleukin-6 . | JOURNAL OF THE ELECTROCHEMICAL SOCIETY , 2025 , 172 (3) . |
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Background: Cardiac troponin I (cTnI) is a crucial diagnosis biomarker for acute myocardial infarction (AMI). Early accurate determination of the concentration of cTnI significantly decreases the death rate of AMI. Compared with classic methods, dual-mode sensors for cTnI determination could help reduce the false positive rate. Result: In this work, an ultrathin Fe-MOF-74 nanosheet with fluorescence properties and peroxidase mimic ac- tivity was synthesized for the first time. Applying this nanosheet, a novel dual-mode sensor was developed to quantify cTnI in human serum. The ultrathin Fe-MOF-74 nanosheets catalyze the decomposition of hydrogen peroxide produced by glucose oxidase (GOx)-triggered enzyme-linked immunosorbent assay (ELISA) applying cTnI as an antigen target, to generate reactive oxygen species (ROS). 3,3 ',5,5 '-tetramethylbenzidine (TMB) can be oxidized by the generated free radicals, which simultaneously lead to the fluorescence quench of Fe-MOF-74 due to the inner filter effect (IFE). The correlation between the morphology of Fe-MOF-74 and its fluorescence intensity and peroxidase mimic activity was also investigated. Significance: The sensors exhibited linearity with the concentration of cTnI in 10-2000 pg mL- 1 in both the fluorescence and visual mode with the detection limit of 6.4 pg mL- 1 and 8.4 pg mL-1, respectively. It presenting good selectivity and anti-interference ability, can provide accurate and precise results in testing the concentration of cTnI in serum samples from patients in hospitals. It could be applied in the early diagnosis of AMI to reduce the incidence, disability, and mortality rates.
Keyword :
Colorimetric Colorimetric cTnI cTnI Dual-mode sensor Dual-mode sensor Early diagnosis Early diagnosis Fe-MOF-74 nanosheet Fe-MOF-74 nanosheet Fluorometric Fluorometric
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GB/T 7714 | Liu, Jinyan , Wang, Yong , Peng, Weijie et al. A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1350 . |
MLA | Liu, Jinyan et al. "A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties" . | ANALYTICA CHIMICA ACTA 1350 (2025) . |
APA | Liu, Jinyan , Wang, Yong , Peng, Weijie , Qiu, Bin , Wong, Kwok-yin , Hu, Shuisheng . A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties . | ANALYTICA CHIMICA ACTA , 2025 , 1350 . |
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IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH2 MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH2 is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL(-1)-1.0 ng mL(-1) coupled with a low detection limit of 0.3 pg mL(-1). It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.
Keyword :
Differential pulse voltammetry Differential pulse voltammetry Electrochemical immunosensor Electrochemical immunosensor Graphdiyne Graphdiyne Interleukin-17 Interleukin-17 MOFs MOFs UiO-66-NH2 UiO-66-NH2
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GB/T 7714 | Li, Rui , Que, Wenzhong , Han, Haiyan et al. Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2 [J]. | MICROCHIMICA ACTA , 2025 , 192 (5) . |
MLA | Li, Rui et al. "Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2" . | MICROCHIMICA ACTA 192 . 5 (2025) . |
APA | Li, Rui , Que, Wenzhong , Han, Haiyan , Shi, Honghui , Wang, Rongfeng , Qiu, Bin . Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2 . | MICROCHIMICA ACTA , 2025 , 192 (5) . |
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Hirschsprung's disease (HSCR), a congenital condition characterized by the absence of nerve cells in the intestinal wall, often requires early and accurate diagnosis for optimal patient outcomes. In this study, we developed a novel and ultrasensitive biosensing strategy for the detection of HSCR-related microRNAs (miRNAs) by integrating catalytic hairpin assembly (CHA) with CRISPR-Cas12a technology. A two-stage process consists of array recognition, and a universal readout is designed. In the first stage, target miRNAs are recognized and amplified on a solid-phase microarray, while in the second stage, the accumulated conversion chains which are not related to target sequences, activate Cas12a, leading to the cleavage of reporter DNA and the generation of a fluorescence signal spatially separated from the first stage. The proposed method was validated for the comprehensive detection of HSCR-related miRNAs and demonstrated high sensitivity and specificity. This work represents a significant advancement in miRNA diagnostics and holds potential for broader clinical applications.
Keyword :
Cas12a Cas12a Catalytic hairpin assembly Catalytic hairpin assembly Fluorescence spectrometry Fluorescence spectrometry Hirschsprung's disease Hirschsprung's disease MicroRNAs MicroRNAs
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GB/T 7714 | Liu, Mingkun , Yan, Lei , Lin, Zhixiong et al. CHA-based microarray with Cas12a universal readout for multiple microRNA detection [J]. | MICROCHIMICA ACTA , 2025 , 192 (5) . |
MLA | Liu, Mingkun et al. "CHA-based microarray with Cas12a universal readout for multiple microRNA detection" . | MICROCHIMICA ACTA 192 . 5 (2025) . |
APA | Liu, Mingkun , Yan, Lei , Lin, Zhixiong , Wu, Dianming , Qiu, Bin , Weng, Shangeng . CHA-based microarray with Cas12a universal readout for multiple microRNA detection . | MICROCHIMICA ACTA , 2025 , 192 (5) . |
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Herein, we present an innovative electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of N-nitrosodimethylamine (NDMA). The biosensor utilizes a triple signal amplification strategy, combining rolling circle amplification (RCA), CRISPR/Cas12a-driven hyperbranched rolling circle amplification (HRCA), and electrostatic repulsion with size exclusion effects from vertically ordered mesoporous silica film (VMSF)/indium tin oxide (ITO) on double-stranded DNA (dsDNA)-Ru(phen)3 2+ complexes. In this system, aptamers and circular DNA undergo RCA reactions, followed by the CRISPR/Cas12a-mediated HRCA process, producing abundant dsDNA. The electropositive ECL indicator, namely Ru(phen)3 2+, was subsequently adsorbed onto the electronegative dsDNA, forming dsDNA-Ru(phen)3 2+ complexes. These complexes are subjected to electrostatic repulsion and size exclusion by the VMSF-modified ITO electrode, resulting in a lower ECL intensity. Upon introducing NDMA, the aptamer preferentially binds to NDMA, thereby preventing the formation of long dsDNA. This process releases free Ru(phen)3 2+, which diffuses to the electrode surface through narrow mesoporous channels via electrostatic adsorption. Consequently, an enhanced and strong ECL signal is observed. The integration of VMSF enhances selectivity and sensitivity by excluding larger impurities and promoting the electrostatic repulsion of dsDNA-Ru(phen)3 2+ complexes near the electrode surface. Additionally, the CRISPR/Cas12a system eliminates the formation of primer dimers and reduces false positives through its unique cis- and trans-cleavage activities. The biosensor demonstrated excellent performance with a linear correlation between the ECL signal and NDMA concentration in the range spanning from 10 pg/mL to 10 mu g/mL, achieving a low limit of detection of 5.33 pg/mL. This platform offers a reliable and robust solution for detecting NDMA in complex matrices, making it a promising tool for environmental monitoring, public health, and safety applications.
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GB/T 7714 | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (10) : 5828-5835 . |
MLA | Zheng, Zhenjie et al. "Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification" . | ANALYTICAL CHEMISTRY 97 . 10 (2025) : 5828-5835 . |
APA | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da , Weng, Zuquan , Wang, Jian , Lin, Cuiying et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification . | ANALYTICAL CHEMISTRY , 2025 , 97 (10) , 5828-5835 . |
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