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学者姓名：黄达
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Abstract ：
Janus hydrogels, distinguished by their dual-sided structure with distinct physical and chemical properties, have garnered significant attention in the medical field, particularly for applications in drug delivery, tissue engineering, and wound healing. Their ability to simultaneously perform multiple functions, such as targeted drug release and biomimetic tissue interaction, positions them as a promising platform for advanced therapeutic strategies. The growing interest in these hydrogels is primarily driven by their multifunctionality and capacity to address complex biological needs. This review delves into the design, fabrication methods, and applications of Janus hydrogels in medicine, focusing on their potential to overcome the limitations of conventional therapies and providing a comprehensive overview of their role in contemporary biomedical applications.
Keyword ：
hemostasis hemostasis Janus hydrogels Janus hydrogels orthopedic repair orthopedic repair smart medical systems smart medical systems tissue engineering tissue engineering
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| GB/T 7714 | Yan, Taoxu , Cheng, Junyao , Liu, Haoming et al. Multifunctional Janus Hydrogels: Surface Design Strategies for Next-Generation Clinical Solutions [J]. | GELS , 2025 , 11 (5) . |
| MLA | Yan, Taoxu et al. "Multifunctional Janus Hydrogels: Surface Design Strategies for Next-Generation Clinical Solutions" . | GELS 11 . 5 (2025) . |
| APA | Yan, Taoxu , Cheng, Junyao , Liu, Haoming , Wang, Yifan , Zhang, Chuyue , Huang, Da et al. Multifunctional Janus Hydrogels: Surface Design Strategies for Next-Generation Clinical Solutions . | GELS , 2025 , 11 (5) . |
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Zearalenone (ZEN) is a common contaminant in crops with serious food safety implications. By leveraging the etching effect of H2O2 on gold nanorods (Au NRs) and the catalysis of Fenton reaction, we have successfully developed two enzyme-linked immunosorbent assay (ELISA) kits with distinct principles. ZEN can be accurately quantified by both methods by measuring changes in the longitudinal surface plasmon resonance (LSPR) absorption peaks of Au NRs. Direct detection kit is simpler and faster, with a linear range of 0.2-4000 ng/mL, while indirect competitive kit is more complex and has a narrower linear range of 0.02-10 ng/mL. This study is expected to provide an effective analytical strategy for rapid screening and accurate monitoring of mold contaminants in food and agricultural products.In this study, a novel dual-mode ELISA system was developed for the detection of zearalenone (ZEN) using Au NRs and their longitudinal surface plasmon resonance (LSPR) properties. By integrating H2O2-mediated nano-etching and Fenton reaction catalysis, the system enables both direct (0.2-4000 ng/mL) and indirect competitive (0.02-10 ng/mL) detection modes. The main work includes: (1) replacing the traditional enzyme colorimetric signal with an LSPR shift for accurate UV-visible quantification and visual multicolor readout; (2) optimizing the nanoenzyme catalytic system for improved reaction sensitivity; (3) eliminating the need for enzyme-labeled secondary antibodies in direct mode, thereby reducing cost and improving stability. The system was validated on grain samples with recoveries of 93.1-107.1% and detection limits of 0.2 ng/mL (direct) and 0.02 ng/mL (indirect). The modular design of the method allows it to be used for the detection of other mycotoxins (e.g., aflatoxin, ochratoxin) and offers great potential for food safety screening, agricultural monitoring, and environmental assessment. This work provides a cost-effective, sensitive and versatile platform for mycotoxin analysis.
Keyword ：
Food safety Food safety Gold nanorods Gold nanorods Visual detection Visual detection Zearalenone Zearalenone
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| GB/T 7714 | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods [J]. | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) : 6665-6676 . |
| MLA | Hu, Yuanlong et al. "A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods" . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION 19 . 9 (2025) : 6665-6676 . |
| APA | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang , Zhong, Yi , Ye, Jialin , Ye, Lingqing et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) , 6665-6676 . |
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Multifunctional hydrogels hold significant promise for promoting the healing of infected wounds but often fall short in inhibiting antibiotic-resistant pathogens, and their clinical translation is limited by complex preparation processes and high costs. In this study, a multifunctional hydrogel is developed by combining metal-phenolic networks (MPNs) formed by tannic acid (TA) and gallium ions (Ga3+) with chitosan (CS) through a simple one-step method. The resulting CS-TA-Ga3+ (CTG) hydrogel is cost-effective and exhibits desirable properties, including injectability, self-healing, pH responsiveness, hemostasis, antioxidant, anti-inflammatory, and antibacterial activities. Importantly, the CTG hydrogels are effective against antibiotic-resistant pathogens due to the unique antibacterial mechanism of Ga3+. In vivo studies demonstrate that the CTG hydrogel promotes follicle formation and collagen deposition, accelerating the healing of infected wounds by inhibiting blood loss, suppressing bacterial growth, and modulating the inflammatory microenvironment. These findings highlight the CTG hydrogel's potential as an advanced and translational dressing for enhancing the healing of infected wounds.
Keyword ：
antibacterial antibacterial gallium ions gallium ions infected wound healing infected wound healing metal-phenolic network metal-phenolic network multifunctional hydrogel multifunctional hydrogel
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| GB/T 7714 | Zou, Minglang , Chen, Cuiping , Wang, Mingda et al. Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing [J]. | ADVANCED HEALTHCARE MATERIALS , 2025 , 14 (9) . |
| MLA | Zou, Minglang et al. "Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing" . | ADVANCED HEALTHCARE MATERIALS 14 . 9 (2025) . |
| APA | Zou, Minglang , Chen, Cuiping , Wang, Mingda , Lei, Chen , Wang, Yongming , Luo, Fang et al. Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing . | ADVANCED HEALTHCARE MATERIALS , 2025 , 14 (9) . |
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This work reports the development and evaluation of dendrimer-based nanogels based on polyamidoamine (PAMAM) dendrimer generation 5, engineered to act as a carrier with reactive oxygen species (ROS)-scavenging capabilities. We developed a cross-linking reaction-enabled flash nanoprecipitation method in which the cross-linking reaction occurs during the flash nanoprecipitation process to form a cross-linked nanostructure. Using this approach, an N-hydroxysuccinimide (NHS)-functionalized ROS-responsive thioketal cross-linker (TK-NHS) was synthesized and utilized to cross-link DAB-core PAMAM dendrimer G5, resulting in the formation of G5-TK nanogels. The resulting nanogels were characterized using dynamic light scattering and transmission electron microscopy, and their cytocompatibility, irritancy, cellular uptake, and ROS scavenging activity were assessed. We confirmed the ROS scavenging capability of these nanogels and observed favorable safety profiles. The G5-TK nanogels can be further developed as carriers for therapeutic delivery applications to treat oxidative stress-related pathological conditions.
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| GB/T 7714 | Qi, Lin , Huang, Da , Kou, Huari et al. Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation [J]. | BIOMACROMOLECULES , 2025 , 26 (5) : 2986-2995 . |
| MLA | Qi, Lin et al. "Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation" . | BIOMACROMOLECULES 26 . 5 (2025) : 2986-2995 . |
| APA | Qi, Lin , Huang, Da , Kou, Huari , Chernatynskaya, Anna , Ercal, Nuran , Yang, Hu . Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation . | BIOMACROMOLECULES , 2025 , 26 (5) , 2986-2995 . |
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Nanoparticle-based therapeutics hold promise for the treatment of atherosclerosis, but challenges such as low drug-loading capacity and a lack of scalable, controllable production hinder their clinical translation. Flash nanoprecipitation, a continuous synthesis method, offers a potential solution for scalable and reproducible nanoparticle production. In this study, we employed a custom-designed multi-inlet vortex mixer to perform cross-linking reaction-enabled flash nanoprecipitation, facilitating controlled and scalable synthesis of cross-linked polyamidoamine (PAMAM) dendrimer nanoparticles. Notably, this approach allows simultaneous nanoparticle cross-linking and drug loading in a single step. The mannose moiety enabled specific targeting of macrophages via mannose receptors, enhancing the localization of the nanoparticles to atherosclerotic plaques. Atorvastatin calcium, a widely used clinical drug for atherosclerosis treatment, was selected as the model drug. This approach achieved both high production rates and high drug-loading capacities, with an output flow rate of 9.6 L/h and a nanoparticle concentration of approximately 0.4 g/L. The optimized formulation exhibited a drug-loading capacity of 37% and an encapsulation efficiency of 76%. In vitro and in vivo experiments demonstrated effective macrophage and plaque targeting, leading to significant therapeutic benefits. Treatment with these nanoparticles resulted in approximately 40% inhibition of aortic root plaque progression compared to the free drug-treated group. This scalable and efficient nanoparticle platform is a promising strategy for improving atherosclerosis treatment.
Keyword ：
atherosclerosis atherosclerosis flash nanoprecipitation flash nanoprecipitation mannose mannose multi-inlet vortex mixer multi-inlet vortex mixer PAMAM dendrimer PAMAM dendrimer
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| GB/T 7714 | Kou, Huari , Qi, Lin , Huang, Da et al. Scalable Fabrication of High-Payload Dendrimer-Based Nanoparticles for Targeted Atherosclerosis Therapy [J]. | ACS APPLIED MATERIALS & INTERFACES , 2025 , 17 (17) : 24953-24962 . |
| MLA | Kou, Huari et al. "Scalable Fabrication of High-Payload Dendrimer-Based Nanoparticles for Targeted Atherosclerosis Therapy" . | ACS APPLIED MATERIALS & INTERFACES 17 . 17 (2025) : 24953-24962 . |
| APA | Kou, Huari , Qi, Lin , Huang, Da , Wu, Jiandong , Shi, Honglan , Yang, Hu . Scalable Fabrication of High-Payload Dendrimer-Based Nanoparticles for Targeted Atherosclerosis Therapy . | ACS APPLIED MATERIALS & INTERFACES , 2025 , 17 (17) , 24953-24962 . |
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The intracellular delivery of protein drugs via nanocarriers offers significant potential for expanding their therapeutic applications. However, the unintended activation of innate immune responses and inflammation triggered by the carriers presents a major challenge, often compromising therapeutic efficacy. Here, we present oligoethylenimine-thioketal (OEI-TK), a reactive oxygen species-responsive cationic polymer with intrinsic anti-inflammatory properties, to overcome this challenge. OEI-TK self-assembles electrostatically with bovine serum albumin (BSA) to form stable nanoparticles (OTB NPs) with excellent encapsulation efficiency. In vitro studies confirmed that OTB NPs retained OEI-TK's antioxidant and anti-inflammatory properties, enhanced biocompatibility, and efficiently delivered BSA into cells. Furthermore, OEI-TK facilitated the intracellular delivery of beta-galactosidase while preserving its enzymatic activity, demonstrating its potential for functional protein transport. These findings highlight OEI-TK as a promising platform with dual benefits of inflammation modulation and intracellular protein delivery, holding potential for the synergistic treatment of inflammation-related diseases.
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| GB/T 7714 | Wang, Yongming , Ming, Yangcan , Yu, Zhichao et al. ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery [J]. | BIOMACROMOLECULES , 2025 , 26 (4) : 2268-2281 . |
| MLA | Wang, Yongming et al. "ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery" . | BIOMACROMOLECULES 26 . 4 (2025) : 2268-2281 . |
| APA | Wang, Yongming , Ming, Yangcan , Yu, Zhichao , Xu, Zhenjin , Zou, Minglang , Chen, Cuiping et al. ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery . | BIOMACROMOLECULES , 2025 , 26 (4) , 2268-2281 . |
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Herein, we present an innovative electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of N-nitrosodimethylamine (NDMA). The biosensor utilizes a triple signal amplification strategy, combining rolling circle amplification (RCA), CRISPR/Cas12a-driven hyperbranched rolling circle amplification (HRCA), and electrostatic repulsion with size exclusion effects from vertically ordered mesoporous silica film (VMSF)/indium tin oxide (ITO) on double-stranded DNA (dsDNA)-Ru(phen)3 2+ complexes. In this system, aptamers and circular DNA undergo RCA reactions, followed by the CRISPR/Cas12a-mediated HRCA process, producing abundant dsDNA. The electropositive ECL indicator, namely Ru(phen)3 2+, was subsequently adsorbed onto the electronegative dsDNA, forming dsDNA-Ru(phen)3 2+ complexes. These complexes are subjected to electrostatic repulsion and size exclusion by the VMSF-modified ITO electrode, resulting in a lower ECL intensity. Upon introducing NDMA, the aptamer preferentially binds to NDMA, thereby preventing the formation of long dsDNA. This process releases free Ru(phen)3 2+, which diffuses to the electrode surface through narrow mesoporous channels via electrostatic adsorption. Consequently, an enhanced and strong ECL signal is observed. The integration of VMSF enhances selectivity and sensitivity by excluding larger impurities and promoting the electrostatic repulsion of dsDNA-Ru(phen)3 2+ complexes near the electrode surface. Additionally, the CRISPR/Cas12a system eliminates the formation of primer dimers and reduces false positives through its unique cis- and trans-cleavage activities. The biosensor demonstrated excellent performance with a linear correlation between the ECL signal and NDMA concentration in the range spanning from 10 pg/mL to 10 mu g/mL, achieving a low limit of detection of 5.33 pg/mL. This platform offers a reliable and robust solution for detecting NDMA in complex matrices, making it a promising tool for environmental monitoring, public health, and safety applications.
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| GB/T 7714 | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (10) : 5828-5835 . |
| MLA | Zheng, Zhenjie et al. "Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification" . | ANALYTICAL CHEMISTRY 97 . 10 (2025) : 5828-5835 . |
| APA | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da , Weng, Zuquan , Wang, Jian , Lin, Cuiying et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification . | ANALYTICAL CHEMISTRY , 2025 , 97 (10) , 5828-5835 . |
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Triple-negative breast cancer (TNBC) accounts for approximately 15% of breast cancers and lacks estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), rendering it unresponsive to hormonal or anti-HER2 therapies. Due to its poor prognosis and limited treatment options, there is an urgent need for targeted therapies. In this study, we developed highly adaptable polyamidoamine (PAMAM) dendrimer-based gel nanoparticles with dual-targeting capabilities against urokinase-type plasminogen activator receptor (uPAR) and ribonucleotide reductase R2 (R2). These nanoparticles were designed to target both TNBC cells and cancer-associated stromal cells by leveraging uPA-uPAR interactions and delivering the antisense oligonucleotide GTI-2040 (GTI) against R2. The resulting dual-functional dendrimer gel nanoparticles, GDP-uPA/GTI, demonstrated good biocompatibility, with an average size of similar to 16.45 nm. GDP-uPA/GTI enhanced GTI delivery by 3.4-fold in TNBC cells (MDA-MB-231) and by 4.8-fold in stromal cells (HCC2218) compared to GTI alone. It reduced R2 expression by 83.1% and induced similar to 30% TNBC cell death. In a TNBC xenograft model, GDP-uPA/GTI significantly inhibited tumor growth by 50.5%. These findings highlight the unique design of the dual-functional dendrimer gel nanoparticles and their dual-targeting efficacy, demonstrating their potential as a promising therapeutic strategy for TNBC.
Keyword ：
activator receptor activator receptor confocal microscopy confocal microscopy nanomedicine nanomedicine ribonucleotide reductase ribonucleotide reductase targeted gene delivery targeted gene delivery
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| GB/T 7714 | Chuang, Hsin-Yin , Huang, Da , Qi, Lin et al. Highly Adaptable Dendrimer Gel Nanoparticles with Dual Targeting of uPAR and Ribonucleotide Reductase R2 for Better Retention and Improved Therapeutic Outcomes in Triple-Negative Breast Cancer [J]. | ACS APPLIED MATERIALS & INTERFACES , 2025 , 17 (23) : 33439-33450 . |
| MLA | Chuang, Hsin-Yin et al. "Highly Adaptable Dendrimer Gel Nanoparticles with Dual Targeting of uPAR and Ribonucleotide Reductase R2 for Better Retention and Improved Therapeutic Outcomes in Triple-Negative Breast Cancer" . | ACS APPLIED MATERIALS & INTERFACES 17 . 23 (2025) : 33439-33450 . |
| APA | Chuang, Hsin-Yin , Huang, Da , Qi, Lin , Singh, Vidit , Chernatynskaya, Anna , Huang, Yue-Wern et al. Highly Adaptable Dendrimer Gel Nanoparticles with Dual Targeting of uPAR and Ribonucleotide Reductase R2 for Better Retention and Improved Therapeutic Outcomes in Triple-Negative Breast Cancer . | ACS APPLIED MATERIALS & INTERFACES , 2025 , 17 (23) , 33439-33450 . |
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Intervertebral disc degeneration (IVDD) stands as the foremost contributor to low back pain (LBP), imposing a substantial weight on the world economy. Traditional treatment modalities encompass both conservative approaches and surgical interventions; however, the former falls short in halting IVDD progression, while the latter carries inherent risks. Hence, the quest for an efficacious method to reverse IVDD onset is paramount. Biomaterial delivery systems, exemplified by hydrogels, microspheres, and microneedles, renowned for their exceptional biocompatibility, biodegradability, biological efficacy, and mechanical attributes, have found widespread application in bone, cartilage, and various tissue engineering endeavors. Consequently, IVD tissue engineering has emerged as a burgeoning field of interest. This paper succinctly introduces the intervertebral disc (IVD) structure and the pathophysiology of IVDD, meticulously classifies biomaterials for IVD repair, and reviews recent advances in the field. Particularly, the strengths and weaknesses of biomaterials in IVD tissue engineering are emphasized, and potential avenues for future research are suggested.
Keyword ：
biomaterials biomaterials intervertebral disc intervertebral disc intervertebral disc degeneration intervertebral disc degeneration tissue engineering tissue engineering
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| GB/T 7714 | Wang, Yifan , Zhang, Chuyue , Cheng, Junyao et al. Cutting-Edge Biomaterials in Intervertebral Disc Degeneration Tissue Engineering [J]. | PHARMACEUTICS , 2024 , 16 (8) . |
| MLA | Wang, Yifan et al. "Cutting-Edge Biomaterials in Intervertebral Disc Degeneration Tissue Engineering" . | PHARMACEUTICS 16 . 8 (2024) . |
| APA | Wang, Yifan , Zhang, Chuyue , Cheng, Junyao , Yan, Taoxu , He, Qing , Huang, Da et al. Cutting-Edge Biomaterials in Intervertebral Disc Degeneration Tissue Engineering . | PHARMACEUTICS , 2024 , 16 (8) . |
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Curcumin (CUR) is a natural polyphenol that holds promise for treating ulcerative colitis (UC), yet oral administration of CUR exhibits limited bioavailability and existing formulations for oral delivery of CUR often suffer from unsatisfactory loading capacity. This study presents hydroxyethyl starch-curcumin microspheres (HCMSs) with excellent CUR loading capacity (54.52 %), and the HC-MSs can further encapsulate anti-inflammatory drugs dexamethasone (DEX) to obtain a combination formulation (DHC-MSs) with high DEX loading capacity (19.91 %), for combination therapy of UC. The microspheres were successfully engineered, retaining the antioxidative and anti-inflammatory activities of parental CUR and demonstrating excellent biocompatibility and controlled release properties, notably triggered by alpha-amylase, facilitating targeted drug delivery to inflamed sites. In a mouse UC model induced by dextran sulfate sodium, the microspheres effectively accumulated in inflamed colons and both HC-MSs and DHC-MSs exhibited superior therapeutic efficacy in alleviating UC symptoms compared to free DEX. Moreover, mechanistic exploration uncovered the multifaceted therapeutic mechanisms of these formulations, encompassing anti-inflammatory actions, mitigation of spleen enlargement, and modulation of gut microbiota composition. These findings underscore the potential of HC-MSs and DHC-MSs as promising formulations for UC, with implications for advancing treatment modalities for various inflammatory bowel disorders.
Keyword ：
Anti-inflammatory Anti-inflammatory Curcumin Curcumin Gut microbiota Gut microbiota Microspheres Microspheres Oral delivery Oral delivery Ulcerative colitis Ulcerative colitis
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| GB/T 7714 | Huang, Da , Wang, Yongming , Xu, Chenlan et al. Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 266 . |
| MLA | Huang, Da et al. "Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 266 (2024) . |
| APA | Huang, Da , Wang, Yongming , Xu, Chenlan , Zou, Minglang , Ming, Yangcan , Luo, Fang et al. Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 266 . |
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