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学者姓名：杨建民
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Abstract ：
The redox homeostasis defense mechanism of tumor cells is one of the prime reasons for the unsatisfactory effect of photodynamic therapy (PDT). So far, little attention has been paid to this obstacle. In this work, we reported a synthesizing simple yet versatile nanogel (BCPS), synthesized by cystamine dihydrochloride functionalized sodium carboxymethylcellulose (CMC-SS), bovine serum albumin, and Phycocyanobilin self-assembly. The BCPS reduced the levels of glutathione molecules by reacting with glutathione, thereby interfering with intracellular redox homeostasis and enhancing the sensitivity of tumor cells to PDT. The BCPS was shown to possess excellent serum stability, high blood compatibility, low toxic side effects, and higher reactive oxygen species (ROS) utilization. After irradiation, the BCPS could significantly increase intracellular ROS level by approximately 1.6-fold and decrease the IC50 to HeLa cells by approximately 1.5-fold, compared to the pre-functional drugs BCP. This proposed strategy, based on increasing the utilization rate of ROS in tumor cells is promising for application potentials in tumor therapy.
Keyword ：
Green self-assembly Green self-assembly Long-circulating Long-circulating Photodynamic therapy Photodynamic therapy Redox-responsive Redox-responsive Tumor-targeted drug delivery Tumor-targeted drug delivery
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| GB/T 7714 | Liao, Wenqiang , Xiao, Siqi , Yang, Jianmin et al. Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy [J]. | CARBOHYDRATE POLYMERS , 2024 , 323 . |
| MLA | Liao, Wenqiang et al. "Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy" . | CARBOHYDRATE POLYMERS 323 (2024) . |
| APA | Liao, Wenqiang , Xiao, Siqi , Yang, Jianmin , Shi, Xianai , Zheng, Yunquan . Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy . | CARBOHYDRATE POLYMERS , 2024 , 323 . |
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The effective repair of large bone defects remains a major challenge due to its limited self-healing capacity. Inspired by the structure and function of the natural periosteum, an electrospun biomimetic periosteum is constructed to programmatically promote bone regeneration using natural bone healing mechanisms. The biomimetic periosteum is composed of a bilayer with an asymmetric structure in which an aligned electrospun poly(epsilon-caprolactone)/gelatin/deferoxamine (PCL/GEL/DFO) layer mimics the outer fibrous layer of the periosteum, while a random coaxial electrospun PCL/GEL/aspirin (ASP) shell and PCL/silicon nanoparticles (SiNPs) core layer mimics the inner cambial layer. The bilayer controls the release of ASP, DFO, and SiNPs to precisely regulate the inflammatory, angiogenic, and osteogenic phases of bone repair. The random coaxial inner layer can effectively antioxidize, promoting cell recruitment, proliferation, differentiation, and mineralization, while the aligned outer layer can promote angiogenesis and prevent fibroblast infiltration. In particular, different stages of bone repair are modulated in a rat skull defect model to achieve faster and better bone regeneration. The proposed biomimetic periosteum is expected to be a promising candidate for bone defect healing. An electrospun biomimetic periosteum with an asymmetric structure is prepared through aligned and coaxial electrospinning for programed promotion of bone regeneration. This biomimetic periosteum exhibits controlled release of multiple agents, enabling regulation of the inflammatory, angiogenic, and osteogenic phases that are essential for bone healing. It demonstrates good efficacy in promoting bone regeneration in a rat skull defect model. image
Keyword ：
asymmetric structure asymmetric structure biomimetic periosteum biomimetic periosteum bone defect bone defect electrospinning electrospinning programmed repair programmed repair
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| GB/T 7714 | Zhao, Xingkai , Zhuang, Yu , Cao, Yongjian et al. Electrospun Biomimetic Periosteum Capable of Controlled Release of Multiple Agents for Programmed Promoting Bone Regeneration [J]. | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (12) . |
| MLA | Zhao, Xingkai et al. "Electrospun Biomimetic Periosteum Capable of Controlled Release of Multiple Agents for Programmed Promoting Bone Regeneration" . | ADVANCED HEALTHCARE MATERIALS 13 . 12 (2024) . |
| APA | Zhao, Xingkai , Zhuang, Yu , Cao, Yongjian , Cai, Fengying , Lv, Yicheng , Zheng, Yunquan et al. Electrospun Biomimetic Periosteum Capable of Controlled Release of Multiple Agents for Programmed Promoting Bone Regeneration . | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (12) . |
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Currently, cisplatin resistance has been recognized as a multistep cascade process for its clinical chemotherapy failure. Hitherto, it remains challenging to develop a feasible and promising strategy to overcome the cascade drug resistance (CDR) issue for achieving fundamentally improved chemotherapeutic efficacy. Herein, a novel self-assembled nanoagent is proposed, which is constructed by Pt(IV) prodrug, cyanine dye (cypate), and gadolinium ion (Gd3+), for systematically conquering the cisplatin resistance by employing near-infrared (NIR) light activated mild-temperature hyperthermia in tumor targets. The proposed nanoagents exhibit high photostability, GSH/H+-responsive dissociation, preferable photothermal conversion, and enhanced cellular uptake performance. In particular, upon 785-nm NIR light irradiation, the generated mild temperature of approximate to 43 degrees C overtly improves the cell membrane permeability and drug uptake, accelerates the disruption of intracellular redox balance, and apparently enhances the formation of Pt-DNA adducts, thereby effectively overcoming the CDR issue and achieves highly improved therapeutic efficacy for cisplatin-resistant tumor ablation. A novel self-assembled nanoagent (Cy-Pt@HA NP) is developed to conquer the cascade drug resistance (CDR) issue of cisplatin. The proposed nanoagent presents NIR light-triggered mild hyperthermia, superior cellular uptake, and tumor-microenvironment-responsive dissociation for effective ablation of drug-resistant tumors, thereby opening a new avenue for addressing CDR issue and maximizing the platinum-based therapeutic efficacy for cancer treatment.image
Keyword ：
cascade cisplatin resistance cascade cisplatin resistance coordination self-assembly coordination self-assembly improved synergistic therapy improved synergistic therapy mild-temperature hyperthermia mild-temperature hyperthermia NIR light-responsive NIR light-responsive
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| GB/T 7714 | Chen, Mingmao , Fu, Yulei , Liu, Yan et al. NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy [J]. | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (11) . |
| MLA | Chen, Mingmao et al. "NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy" . | ADVANCED HEALTHCARE MATERIALS 13 . 11 (2024) . |
| APA | Chen, Mingmao , Fu, Yulei , Liu, Yan , Zhang, Baihe , Song, Xiaorong , Chen, Xinchun et al. NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy . | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (11) . |
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Abdominal and intrauterine adhesions are common postoperative problems that can cause serious complications. Current adhesives are usually double sided and suffer from poor wet adhesion, nondegradability, and monofunctionality, which limits their application in preventing postoperative adhesions. Herein, a bioinspired microstructured Janus bioadhesive, named OD/GM@PG, with a wet adhesive inner layer and an antiadhesive outer layer is prepared by combining electrostatic spun and adhesive materials. By using both capillary suction and a catechol-based strategy, the wet adhesive strength and interfacial toughness of the Janus bioadhesive reach 98 kPa and 325 J m-2, respectively, which are much higher than those of commercial fibrin glues and cyanoacrylate glues. The electrostatic spun outer layer acts as a physical barrier with antiadhesive and friction-reducing effects. Additionally, the Janus bioadhesive demonstrates biodegradable, hemostatic, antioxidative, anti-inflammatory, and prohealing properties. In vivo results show that the asymmetric adhesion effect of the Janus bioadhesive effectively preventing postoperative abdominal and intrauterine adhesions. Notably, tandem mass tags-labeled quantitative proteomics analysis demonstrate that the expression of inflammatory response-associated proteins (S100A8, S100A9) is associated with adhesion; the Janus bioadhesive significantly downregulates this expression. Therefore, the OD/GM@PG Janus bioadhesive is a promising candidate for preventing postoperative adhesions. A bioinspired microstructured Janus bioadhesive with an asymmetric structure and adhesion behavior is prepared by combining electrostatic spinning and adhesive materials. By employing capillary suction and a catechol-based strategy, the Janus bioadhesive exhibits impressive wet adhesive strength and interfacial toughness. Furthermore, the asymmetric adhesion effect of the Janus bioadhesive effectively alleviates postoperative abdominal and intrauterine adhesions. image
Keyword ：
capillary suction capillary suction fertility restoration fertility restoration Janus bioadhesive Janus bioadhesive preventing postoperative adhesion preventing postoperative adhesion wet adhesion wet adhesion
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| GB/T 7714 | Lv, Yicheng , Cai, Fengying , Zhao, Xingkai et al. Bioinspired Microstructured Janus Bioadhesive for the Prevention of Abdominal and Intrauterine Adhesions [J]. | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (21) . |
| MLA | Lv, Yicheng et al. "Bioinspired Microstructured Janus Bioadhesive for the Prevention of Abdominal and Intrauterine Adhesions" . | ADVANCED FUNCTIONAL MATERIALS 34 . 21 (2024) . |
| APA | Lv, Yicheng , Cai, Fengying , Zhao, Xingkai , Zhu, Xintao , Wei, Fanan , Zheng, Yunquan et al. Bioinspired Microstructured Janus Bioadhesive for the Prevention of Abdominal and Intrauterine Adhesions . | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (21) . |
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The management of severe full-thickness skin defect wounds remains a challenge due to their irregular shape, uncontrollable bleeding, high risk of infection, and prolonged healing period. Herein, an all-in-one OD/GM/QCS@Exo hydrogel was prepared with catechol-modified oxidized hyaluronic acid (OD), methylacrylylated gelatin (GM), and quaternized chitosan (QCS) and loaded with adipose mesenchymal stem cell-derived exosomes (Exos). Cross-linking of the hydrogel was achieved using visible light instead of ultraviolet light irradiation, providing injectability and good biocompatibility. Notably, the incorporation of catechol groups and multicross-linked networks in the hydrogels conferred strong adhesion properties and mechanical strength against external forces such as tensile and compressive stress. Furthermore, our hydrogel exhibited antibacterial, anti-inflammatory, and antioxidant properties along with wound-healing promotion effects. Our results demonstrated that the hydrogel-mediated release of Exos significantly promotes cellular proliferation, migration, and angiogenesis, thereby accelerating skin structure reconstruction and functional recovery during the wound-healing process. Overall, the all-in-one OD/GM/QCS@Exo hydrogel provided a promising therapeutic strategy for the treatment of full-thickness skin defect wounds through actively participating in the entire process of wound healing.
Keyword ：
all-in-one all-in-one exosomes exosomes multifunctionalhydrogel multifunctionalhydrogel quaternized chitosan quaternized chitosan visible light cross-linkable visible light cross-linkable
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| GB/T 7714 | Lv, Yicheng , Li, Liang , Zhang, Jingyuan et al. Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process [J]. | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (20) : 25923-25937 . |
| MLA | Lv, Yicheng et al. "Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process" . | ACS APPLIED MATERIALS & INTERFACES 16 . 20 (2024) : 25923-25937 . |
| APA | Lv, Yicheng , Li, Liang , Zhang, Jingyuan , Li, Jingsi , Cai, Fengying , Huang, Yufeng et al. Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process . | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (20) , 25923-25937 . |
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Tissue engineering scaffolds with tunable viscoelasticity and adaptability for cell behavior and fate regulation are highly desired. Here a dynamic interpenetrating polymer network (IPN) hydrogel was fabricated via photopolymerization and oxidation of methacryloyl gelatin (GelMA) and hyaluronic acid (HASH). The permanent GelMA network formed by C-C bonds provides stable support for cells while the dynamic HASH network formed by disulfide bonds provides an adaptable microenvironment for cell growth. The proposed IPN hydrogel exhibits extensive and tunable porosity, swelling, degradation, and mechanical properties. Remarkably, the dynamic IPN hydrogel mimics the viscoelasticity and adaptability of the extracellular matrix (ECM), which can regulate cellular behaviors such as morphogenesis, alignment, proliferation, migration while offering resistance to cell mediated shrinkage and enzymatic digestion, maintaining the structural integrity of the scaffold. Our results suggest that dynamic IPN 3/7 (HASH/GelMA) hydrogels had more similar physical properties to human skin and were more favorable for human skin fibroblasts (HSF) and human immortalized keratinocytes (HaCaT) growth. Moreover, bilayer tissue-engineered skin prepared using the dynamic IPN hydrogel exhibited satisfactory mechanical stability, dermal-epidermal stratification, matrix secretion, structural differentiation, and barrier functions. In addition, the bilayer tissue-engineered skin can significantly promote healing of full-thickness skin defects through accelerated wound re-epithelialization, collagen deposition, and angiogenesis, without causing non-specific or specific immune rejection. This work based on the novel dynamic IPN hydrogel with biomimetic viscoelasticity and adaptability demonstrates the promising application in tissue engineering.
Keyword ：
Adaptable hydrogel Adaptable hydrogel Cell scaffold Cell scaffold Interpenetrating network Interpenetrating network Tissue-engineered skin Tissue-engineered skin
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| GB/T 7714 | Wang, Weibin , Dai, Jiajia , Huang, Yufeng et al. Extracellular matrix mimicking dynamic interpenetrating network hydrogel for skin tissue engineering [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 457 . |
| MLA | Wang, Weibin et al. "Extracellular matrix mimicking dynamic interpenetrating network hydrogel for skin tissue engineering" . | CHEMICAL ENGINEERING JOURNAL 457 (2023) . |
| APA | Wang, Weibin , Dai, Jiajia , Huang, Yufeng , Li, Xiaomeng , Yang, Jianmin , Zheng, Yunquan et al. Extracellular matrix mimicking dynamic interpenetrating network hydrogel for skin tissue engineering . | CHEMICAL ENGINEERING JOURNAL , 2023 , 457 . |
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Persistent oxidative stress and recurring waves of inflammation with excessive reactive oxygen species (ROS) and free radical accumulation could be generated by radiation. Exposure to radiation in combina-tion with physical injuries such as wound trauma would produce a more harmful set of medical com-plications, which was known as radiation combined with skin wounds (RCSWs). However, little atten-tion has been given to RCSW research despite the unsatisfactory therapeutic outcomes. In this study, a dual-nanoagent-loaded multifunctional hydrogel was fabricated to ameliorate the pathological microen-vironment associated with RCSWs. The injectable, adhesive, and self-healing hydrogel was prepared by crosslinking carbohydrazide-modified gelatin (Gel-CDH) and oxidized hyaluronic acid (OHA) through the Schiff-base reaction under mild condition. Polydopamine nanoparticles (PDA-NPs) and mesenchymal stem cell-secreted small extracellular vesicles (MSC-sEV) were loaded to relieve radiation-produced tissue in-flammation and oxidation impairment and enhance cell vitality and angiogenesis individually or jointly. The proposed PDA-NPs@MSC-sEV hydrogel enhanced cell vitality, as shown by cell proliferation, migra-tion, colony formation, and cell cycle and apoptosis assays in vitro , and promoted reepithelization by at-tenuating microenvironment pathology in vivo . Notably, a gene set enrichment analysis of proteomic data revealed significant enrichment with adipogenic and hypoxic pathways, which play prominent roles in wound repair. Specifically, target genes were predicted based on differential transcription factor expres-sion. The results suggested that MSC-sEV-and PDA-NP-loaded multifunctional hydrogels may be promis-ing nanotherapies for RCSWs.Statement of significanceThe small extracellular vesicle (sEV) has distinct advantages compared with MSCs, and polydopamine nanoparticles (PDA-NPs), known as the biological materials with good cell affinity and histocompatibility which have been reported to scavenge ROS free radicals. In this study, an adhesive, injectable, self-healing, antibacterial, ROS scavenging and amelioration of the radiation related microenvironment hydrogel en-capsulating nanoscale particles of MSC-sEV and PDA-NPs (PDA-NPs@MSC-sEV hydrogel) was synthesized
Keyword ：
Extracellular vesicles Extracellular vesicles Hydrogel Hydrogel Nanomedicine Nanomedicine Polydopamine nanoparticles Polydopamine nanoparticles Radiation combined with skin wounds Radiation combined with skin wounds
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| GB/T 7714 | Fang, Zhuoqun , Lv, Yicheng , Zhang, Haoruo et al. A multifunctional hydrogel loaded with two nanoagents improves the pathological microenvironment associated with radiation combined with skin wounds [J]. | ACTA BIOMATERIALIA , 2023 , 159 : 111-127 . |
| MLA | Fang, Zhuoqun et al. "A multifunctional hydrogel loaded with two nanoagents improves the pathological microenvironment associated with radiation combined with skin wounds" . | ACTA BIOMATERIALIA 159 (2023) : 111-127 . |
| APA | Fang, Zhuoqun , Lv, Yicheng , Zhang, Haoruo , Zhang, Yuxiang , Gao, Hangqi , Chen, Caixiang et al. A multifunctional hydrogel loaded with two nanoagents improves the pathological microenvironment associated with radiation combined with skin wounds . | ACTA BIOMATERIALIA , 2023 , 159 , 111-127 . |
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Cell surface engineering technologies can regulate cell function and behavior by modifying the cell surface. Previous studies have mainly focused on investigating the effects of cell surface engineering reactions and materials on cell activity. However, they do not comprehensively analyze other cellular processes. This study exploits covalent bonding, hydrophobic interactions, and electrostatic interactions to modify the macromolecules succinimide ester-methoxy polyethylene glycol (NHS-mPEG), distearoyl phosphoethanolamine-methoxy polyethylene glycol (DSPE-mPEG), and poly-L-lysine (PLL), respectively, on the cell surface. This work systematically investigates the effects of the three surface engineering reactions on the behavior of human umbilical vein endothelial cells (HUVECs) and human skin fibroblasts, including viability, growth, proliferation, cell cycle, adhesion, and migration. The results reveals that the PLL modification method notably affects cell viability and G2/M arrest and has a short modification duration. However, the DSPE-mPEG and NHS-mPEG modification methods have little effect on cell viability and proliferation but have a prolonged modification duration. Moreover, the DSPE-mPEG modification method highly affects cell adherence. Further, the NHS-mPEG modification method can significantly improve the migration ability of HUVECs by reducing the area of focal adhesions. The findings of this study will contribute to the application of cell surface engineering technology in the biomedical field.
Keyword ：
cell biological behavior cell biological behavior cell surface engineering cell surface engineering covalent bonding covalent bonding electrostatic interaction electrostatic interaction hydrophobic interaction hydrophobic interaction
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| GB/T 7714 | Cai, Fengying , Ren, Yafeng , Dai, Jiajia et al. Effects of Various Cell Surface Engineering Reactions on the Biological Behavior of Mammalian Cells [J]. | MACROMOLECULAR BIOSCIENCE , 2023 , 23 (3) . |
| MLA | Cai, Fengying et al. "Effects of Various Cell Surface Engineering Reactions on the Biological Behavior of Mammalian Cells" . | MACROMOLECULAR BIOSCIENCE 23 . 3 (2023) . |
| APA | Cai, Fengying , Ren, Yafeng , Dai, Jiajia , Yang, Jianmin , Shi, Xianai . Effects of Various Cell Surface Engineering Reactions on the Biological Behavior of Mammalian Cells . | MACROMOLECULAR BIOSCIENCE , 2023 , 23 (3) . |
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The simultaneous regeneration of articular cartilage and subchondral bone is a major challenge. Bioinspired scaffolds with distinct regions resembling stratified anatomical architecture provide a potential strategy for osteochondral defect repair. Here, we report the development of an injectable and bilayered hydrogel scaffold with a strong interface binding force. In this bilayer hydrogel, composed of carbonyl hydrazide grafted collagen (COL-CDH) and oxidized chondroitin sulfate (OCS), which are derivatives of osteochondral tissue components, in combination with poly (ethylene glycol) diacrylate (PEGDA), functions as a cartilage layer; while zinc-doped hydroxyapatite acts as a subchondral bone layer that is based on the cartilage layer. The strong interface be-tween the two layers involves dynamic amide bonds formed between COL-CDH and OCS, and permanent C-C bonds formed by PEGDA radical reactions. This bilayer hydrogel can be used to inoculate adipose mesenchymal stem cells which can then differentiate into chondrocytes and osteoblasts, secreting glycosaminoglycan, and promoting calcium deposition. This accelerates the regeneration of cartilage and subchondral bone. Micro-CT and tissue staining revealed an increase in the amount of bone present in new subchondral bone, and new tis-sues with a structure similar to normal cartilage. This study therefore demonstrates that injectable bilayer hydrogels are a promising scaffold for repairing osteochondral defects.
Keyword ：
Adipose-derived mesenchymal stem cells Adipose-derived mesenchymal stem cells Bilayered scaffold Bilayered scaffold Biomimetic Biomimetic Injectable hydrogel Injectable hydrogel Osteochondral regeneration Osteochondral regeneration
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| GB/T 7714 | Cao, Yongjian , Zhang, Haijie , Qiu, Mengjie et al. Biomimetic injectable and bilayered hydrogel scaffold based on collagen and chondroitin sulfate for the repair of osteochondral defects [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 257 . |
| MLA | Cao, Yongjian et al. "Biomimetic injectable and bilayered hydrogel scaffold based on collagen and chondroitin sulfate for the repair of osteochondral defects" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 257 (2023) . |
| APA | Cao, Yongjian , Zhang, Haijie , Qiu, Mengjie , Zheng, Yunquan , Shi, Xianai , Yang, Jianmin . Biomimetic injectable and bilayered hydrogel scaffold based on collagen and chondroitin sulfate for the repair of osteochondral defects . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 257 . |
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Biomechanical stimuli derived from the extracellular matrix (ECM) extremely tune stem cell fate through 3D and spatiotemporal changes in vivo. The matrix stiffness is a crucial factor during bone tissue development. However, most in vitro models to study the osteogenesis of mesenchymal stem cells (MSCs) are static or stiffening in a 2D environment. Here, a dynamic and controllable stiffening 3D biomimetic model is created to regulate the osteogenic differentiation of MSCs with a dual-functional gelatin macromer that can generate a double-network hydrogel by sequential enzymatic and light-triggered crosslinking reactions. The findings show that these dynamic hydrogels allowed cells to spread and expand prior to the secondary crosslinking and to sense high stiffness after stiffening. The MSCs in the dynamic hydrogels, especially the hydrogel stiffened at the late period, present significantly elevated osteogenic ECM secretion, gene expression, and nuclear localization of Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ). In vivo evaluation of animal experiments further indicates that the enhancement of dynamic stiffening on osteogenesis of MSCs substantially promotes bone remodeling. Consequently, this work reveals that the 3D dynamic stiffening microenvironment as a critical biophysical cue not only mediates the stem cell fate in vitro, but also augments bone restoration in vivo. Dynamic stiffening matrix as a biomimetic niche plays a pivotal role in modulating stem cell fates. This work develops a dynamic stiffening hydrogel system to manipulate the multiple cellular activities of MSCs and highlights the role of stiffening to construct a tissue engineering matrix with controllable dynamic capacity.image
Keyword ：
biomechanical cues biomechanical cues controllable stiffening timing controllable stiffening timing double-network hydrogels double-network hydrogels dynamic stiffening dynamic stiffening mesenchymal stem cells mesenchymal stem cells osteogenic differentiation osteogenic differentiation
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| GB/T 7714 | Li, Xiaomeng , Liu, Shuaibing , Han, Shanshan et al. Dynamic Stiffening Hydrogel with Instructive Stiffening Timing Modulates Stem Cell Fate In Vitro and Enhances Bone Remodeling In Vivo [J]. | ADVANCED HEALTHCARE MATERIALS , 2023 , 12 (29) . |
| MLA | Li, Xiaomeng et al. "Dynamic Stiffening Hydrogel with Instructive Stiffening Timing Modulates Stem Cell Fate In Vitro and Enhances Bone Remodeling In Vivo" . | ADVANCED HEALTHCARE MATERIALS 12 . 29 (2023) . |
| APA | Li, Xiaomeng , Liu, Shuaibing , Han, Shanshan , Sun, Qingqing , Yang, Jianmin , Zhang, Yuhang et al. Dynamic Stiffening Hydrogel with Instructive Stiffening Timing Modulates Stem Cell Fate In Vitro and Enhances Bone Remodeling In Vivo . | ADVANCED HEALTHCARE MATERIALS , 2023 , 12 (29) . |
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