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学者姓名：林陈晨
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Abstract ：
Environmental smog pollution induces chronic obstructive pulmonary disease (COPD), and causes the urgent need of timely, safe and quantitative testing for risk assessment. The miRNA-based bioanalysis offers a non-invasive access to rapid COPD screening, while challenges remain in accurately identifying miRNAs from complex matrices. Herein, a novel bionic aptasensor has been pioneered for meeting the specific and sensitive detection of the miR-155 in serum for COPD diagnosis, integrating tetrahedral framework nucleic acid (tFNA) confined affinity recognition and enzyme-free hybridization chain reaction (HCR) amplification strategy. Precision-designed tFNAs were fabricated and hybridized with FAM-labeled cDNAs, enabling dual-mode recognition mechanism including spatially confined aptamer recognition and bionic molecular sieve size-selective action. Proteins and pre-miRNAs were excluded, and the target microRNA-155 was specifically captured. This triggered a structural switch of tFNAs and activated cDNA-mediated HCR, assembling double-stranded DNA and achieving exponential fluorescence enhancement synergistically produced by SYBR Green I (SGI) and FAM. The optimal limit of detection as 53 fM for miRNA-155 was achieved with laser-induced fluorescence (LIF). Applied to COPD diagnosis, the detections of trace miRNA-155 were satisfactory and the recovery yields were ranged in 96.4 +/- 5.2 % similar to 106.2 +/- 2.1 % (n = 3) in the serum of COPD patients. Smoke-exposed model rats were also analyzed, and the miRNA-155 was achieved with a significant concentration up-regulation to light the COPD. It offers a high-performance protocol for meeting the non-invasive rapid screening of COPD, further providing promising technologies for evaluating safety issues of environmentally induced diseases.
Keyword ：
Confined affinity recognition Confined affinity recognition COPD COPD HCR HCR miRNA155 miRNA155 Tetrahedral framework nucleic acid Tetrahedral framework nucleic acid
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| GB/T 7714 | Huang, Guobin , Xu, Weiting , Chi, Jinxin et al. tFNA-aptasensor integrating DNA-framework confined affinity recognition and HCR amplification towards accurate profiling of microRNA for COPD analysis [J]. | MICROCHEMICAL JOURNAL , 2025 , 216 . |
| MLA | Huang, Guobin et al. "tFNA-aptasensor integrating DNA-framework confined affinity recognition and HCR amplification towards accurate profiling of microRNA for COPD analysis" . | MICROCHEMICAL JOURNAL 216 (2025) . |
| APA | Huang, Guobin , Xu, Weiting , Chi, Jinxin , Lin, Chenchen , Wang, Jiabin , Lin, Xucong . tFNA-aptasensor integrating DNA-framework confined affinity recognition and HCR amplification towards accurate profiling of microRNA for COPD analysis . | MICROCHEMICAL JOURNAL , 2025 , 216 . |
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Surface engineering of covalent organic frameworks (COFs) represents a highly promising strategy for achieving superior capture capacity towards environmental trace pollutants. Herein, the three-dimensional covalent organic frameworks (3D-COFs) with tunable crystallinity were synthesized and optimized via a simple solvent ratio adjustment, demonstrating an exceptional capture capacity of polychlorinated biphenyls (PCBs). By adjusting the ratio of solvents (1,4-dioxane and toluene), a functional 3D COF (dia-c5 TPA-TAM-COF) was discovered, providing an ideal approach to transform an amorphous compound into a functional platform with a significant increase in crystallinity, surface area and pore volume for PCBs capture. The adsorption features and analytical performance of PCBs were evaluated, and a good performance was achieved with high enrichment factors as 6973 similar to 11,015. Optimization of solid phase microextraction (SPME) using the dia-c5 TPA-TAM-COF as the coating was investigated, and the method was verified for sensitive quantification of PCBs by using GC-MS, achieving the sensitive limits of detection (LODs) of 0.028 similar to 0.0263 ng/L. Good reproducibility and robustness of the analytical performance of PCBs using dis-c5 was achieved, with the RSDs of intra-day (n = 3), inter-day (n = 6) and fiber-to-fiber (n = 3) of 0.7 similar to 4.7 %, 1.8 similar to 4.6 % and 2.0 similar to 3.9 % respectively. The recoveries of PCBs in the soil fortified samples were obtained as 85.3 similar to 101.9 % and 86.3 similar to 108.8 %, respectively, as well as high thermal stability and long lifetime (keep on going more than 150 cycles). This study demonstrates that a simple protocol of adjusting the solvent content was capable of tailoring high crystallinity and suitable interpenetration degree of TPA-TAM-COFs for high performance target capture of PCBs in environmental soils.
Keyword ：
3D covalent organic frameworks 3D covalent organic frameworks Crystalline modulation Crystalline modulation Polychlorinated biphenyls Polychlorinated biphenyls Soils Soils Surface engineering Surface engineering
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| GB/T 7714 | Chen, Qingai , Lu, Feifei , Chen, Chenqun et al. Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls [J]. | JOURNAL OF CHROMATOGRAPHY A , 2025 , 1755 . |
| MLA | Chen, Qingai et al. "Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls" . | JOURNAL OF CHROMATOGRAPHY A 1755 (2025) . |
| APA | Chen, Qingai , Lu, Feifei , Chen, Chenqun , Lin, Chenchen , Wang, Jiabin , Lin, Xucong et al. Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls . | JOURNAL OF CHROMATOGRAPHY A , 2025 , 1755 . |
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Developing a functional media for precise identification of trace shellfish toxin would underpin the effective assessment of marine pollution. Herein, a novel monolithic column with a dual-mode strategy integrating antifouling and aptamer bionic affinity recognition was proposed for online specific identification of the marine toxin okadaic acid (OA). The zwitterionic monomer 2-(methacryloyloxy)ethylphosphorylcholine (MPC) and aptamers were synergistically employed to enable efficient reduction of matrix interferences and selective capture of target OA. Preparation optimization, characterization, and fouling-resistant mechanism of the dualmode bionic monolith were evaluated. The zwitterion phosphorylcholine MPC introduced into the monolith significantly improved the fouling resistance to biomass substrates, meanwhile the aptamers were able to provide a high specific recognition capacity. Coupled with LC-MS, the as-prepared monolith provided an effective approach for highly selective and sensitive identification of OA. Good recovery yields of over 90 % in shellfish tissue extracts and human serum were achieved with a sensitive limit of detection (LOD) as low as 0.1 ng/mL, as well as excellent specificity and low interference from proteins, fatty acids and analogues. Applied to popular shellfishes (such as clams, mussels, and oysters) and serum samples, trace OA toxin was accurately distinguished and quantified with satisfactory recoveries as 93.8 +/- 2.2 % - 99.9 +/- 1.9 % (n = 3). Compared to the traditional HLB cartridge and other materials in the LC-MS method, the resulting anti-fouling aptamer monolith provided a more advanced online analysis mode with higher sensitivity and better resolution of OA in biological samples. It might provide an attractive access to an online bionic recognition platform with LC-MS for efficient, antiinterference and sensitive specific detection of trace marine toxin OA in biological samples.
Keyword ：
Aptamer affinity monolith Aptamer affinity monolith Dual mode Dual mode Okadaic acid Okadaic acid Online recognition Online recognition Zwitterion antifouling Zwitterion antifouling
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| GB/T 7714 | Peng, Qi , Ye, Yan , Wu, Xinglin et al. Dual-mode monolithic column with zwitterion antifouling and aptamer affinity for online specific recognition of okadaic acid [J]. | JOURNAL OF CHROMATOGRAPHY A , 2025 , 1747 . |
| MLA | Peng, Qi et al. "Dual-mode monolithic column with zwitterion antifouling and aptamer affinity for online specific recognition of okadaic acid" . | JOURNAL OF CHROMATOGRAPHY A 1747 (2025) . |
| APA | Peng, Qi , Ye, Yan , Wu, Xinglin , Lin, Chenchen , Lin, Xucong , Weng, Qibiao et al. Dual-mode monolithic column with zwitterion antifouling and aptamer affinity for online specific recognition of okadaic acid . | JOURNAL OF CHROMATOGRAPHY A , 2025 , 1747 . |
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Background: Enzyme-linked immunosorbent assay (ELISA) requires the simultaneous maintenance of enzyme activity and the efficient labelling of the enzymes on the antibodies. Challenges in ELISA include the enzyme decomposition under harsh conditions and the requirement for secondary antibodies for binding. Preserving enzyme activity and simplifying antibody binding is a crucial prerequisite for expanding the applications of ELISA. Most encapsulation cases focus on enzyme immobilization in MOFs/COFs, while the synergetic encapsulation of enzyme-biotin in DNA nanoflowers (DNFs) and their labeling of capture antibodies to construct stable streamlined ELISA platforms was few. It is clear that a novel protocol is needed that is facile, capable of preserving both high enzymatic activity and efficient antibody binding for the stable ELISA. Results: We proposed a novel horseradish peroxidase (HRP)/biotin co-encapsulated DNA nanoflowers (HRP/ biotin@DNFs) for the synergistic implementation of the robust enzyme immobilization using rolling circle amplification and secondary antibody-free antibody binding using biotin-streptavidin ligation in ELISA. Enzyme immobilization, abundant antibody binding sites, and superior protection of enzymes were achieved in synchrony. High enzyme activity, excellent stability and chemical tolerance of HRP/biotin@DNFs were obtained. Especially, HRP/biotin@DNFs provides 95 %-85 % and 91 %-81 % of its original activity after the exposure at 25 degrees C and 37 degrees C in 5 days, respectively, whereas that of free HRP was drastically reduced to only 40 % and 20 % after the exposure at 25 degrees C and 37 degrees C for 5 days, respectively. Using tetrabromobisphenol A bis-(2-hydroxyethyl) ether (viz. TBBPA-BHEE) as the target model, high performance for the assay was achieved, with high enzymatic activity, good tolerance and sensitive limit of detection of 0.66 ng mL-1 that was 5-fold more sensitive than that of common ELISA. Stable and accurate ELISA for TBBPA-BHEE was realized with recovery yields of 91.4 %- 112.8 % with RSD at 1.6-3.4 %. Significance: This study pioneers a simple yet powerful strategy that simultaneously achieves both affordable, biologically encapsulated enzymes with high activity and secondary antibody-free antibody conjugation. It expands the toolbox of enzyme immobilization and facilitates enzyme binding on antibody without the need of secondary antibody, enabling cost-effective and robust ELISA for environmental assays.
Keyword ：
Biotin-streptavidin interaction Biotin-streptavidin interaction DNA nanoflowers DNA nanoflowers ELISA ELISA Enzyme/biotin encapsulation Enzyme/biotin encapsulation Rolling circle amplification Rolling circle amplification
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| GB/T 7714 | Wu, Xinglin , Chen, Chengqun , Lin, Chenchen et al. Synergistic HRP/Biotin encapsulation in DNA nanoflowers via RCA: A path to stabilized, streamlined ELISA platform [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1371 . |
| MLA | Wu, Xinglin et al. "Synergistic HRP/Biotin encapsulation in DNA nanoflowers via RCA: A path to stabilized, streamlined ELISA platform" . | ANALYTICA CHIMICA ACTA 1371 (2025) . |
| APA | Wu, Xinglin , Chen, Chengqun , Lin, Chenchen , Wang, Jiabin , Weng, Qibiao , Lin, Xucong . Synergistic HRP/Biotin encapsulation in DNA nanoflowers via RCA: A path to stabilized, streamlined ELISA platform . | ANALYTICA CHIMICA ACTA , 2025 , 1371 . |
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The release of microcystin (MCs) in aquatic ecosystems poses a substantial risk to the safety of irrigation and drinking water. In view of the challenges associated with monitoring MCs in water bodies, given their low concentration levels (mu g/L to ng/L) and the presence of diverse matrix interferences, there is an urgent need to develop an efficient, cost-effective and selective enrichment technique for MCs prior to its quantification. In this work, a gold nanoparticles (AuNPs)-functionalized zwitterionic polymer monolith was described and further applied for the affinity enrichment of MCs. Monoliths modified with zwitterionic amino acid ligands were synthesized within capillary microchannels as highly permeable porous supports for the immobilization of gold nanoparticles via electrostatic interactions. The structure and morphology of AuNPs-hybrid monoliths were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and FT-IR spectroscopy, confirming the uniform loading of AuNPs on the polymer matrix while ensuring porous structural stability. The asprepared hybrid monolith was employed as a sorbent for capillary microextraction (CME) prior to liquid chromatography-mass spectrometry (LC-MS) analysis. The results demonstrated that the functionalization of AuNPs under the protection of zwitterionic polymers markedly enhanced the extraction ability of the monolithic column for MCs. This is attributed to the formation of ligand exchange, hydrophobic interactions, and It-system interactions between MCs and AuNPs, as verified by zeta potential and XPS characterization. The developed CME-LC-MS method exhibited a wide linear range (1-10,000 ng L- 1), a low limit of detection (0.58-1.6 ng L- 1), and satisfactory recoveries (71.0-126.7 %) for three MCs in different ambient water sample matrices. It is evident from these findings that AuNPs-functionalized zwitterionic polymers represent a promising novel material for the efficient adsorption of MCs from aqueous samples.
Keyword ：
Affinity adsorbent Affinity adsorbent Capillary microextraction Capillary microextraction Gold nanoparticles Gold nanoparticles Microcystins Microcystins Zwitterionic polymer Zwitterionic polymer
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| GB/T 7714 | Wang, Shuqiang , Huang, Jiansong , Huang, Ting et al. Gold nanoparticles supported onto zwitterionic polymer capillary monoliths meant for efficient enrichment of microcystins in water [J]. | TALANTA , 2025 , 286 . |
| MLA | Wang, Shuqiang et al. "Gold nanoparticles supported onto zwitterionic polymer capillary monoliths meant for efficient enrichment of microcystins in water" . | TALANTA 286 (2025) . |
| APA | Wang, Shuqiang , Huang, Jiansong , Huang, Ting , Huang, Guobin , Lin, Chenchen , Wu, Xiaoping . Gold nanoparticles supported onto zwitterionic polymer capillary monoliths meant for efficient enrichment of microcystins in water . | TALANTA , 2025 , 286 . |
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Developing high-performance lateral flow immunoassays (LFIA) for portable and rapid profiling equipment is a key to meet the field-testing of hazardous pollutants. Tetrabromobisphenol A bis (2-hydroxyethyl) ether (TBBPABHEE) as an emerging hazardous contaminant poses potential risks to human health, yet there is no rapid, specific or sensitive LFIA for monitoring purpose. Herein, a novel dual-mode LFIA for field testing TBBPA-BHEE was pioneered with colorimetric/diffuse reflection using gold nanoflowers (AuNFs) and monoclonal antibody (AuNFs@mAb). Initially, a functional monoclonal antibody (mAb) was prepared and purified. The mAb was subsequently labeled on AuNFs to create the AuNFs@mAb probe, which could be captured by TBBPA-BHEE molecule in 10 min and achieved colorimetric/diffuse reflectance spectroscopy using naked eyes or portable immunochromato-reader, respectively. The preparation of test strips was systematically studied and the LFIA for qualitative and quantitative analysis of TBBPA-BHEE were evaluated. The optimized LFIA achieved a visual limit of detection (LOD) of 5.0 ng/mL and a detection LOD of 0.25 ng/mL by portable immunochromato-reader. It showed a high specificity for TBBPA-BHEE, good recovery yields of 112.41-120.10 % with the relative standard deviation (RSD) less than 8.0 %, and excellent long-term stability. Moreover, the results of the resultant LFIA were highly consistent with that of HPLC method, with a correlation coefficient of 0.995. Therefore, this study highlights the promising application in developing an economical and selective LFIA to field profiling of TBBPABHEE.
Keyword ：
Dual-mode Dual-mode Gold nanoflowers Gold nanoflowers Lateral flow immunoassay Lateral flow immunoassay Monoclonal antibody Monoclonal antibody Tetrabromobisphenol A bis (2-hydroxyethyl) ether Tetrabromobisphenol A bis (2-hydroxyethyl) ether
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| GB/T 7714 | Wu, Xinglin , Wang, Leding , He, Youfen et al. Dual-mode lateral flow immunoassay for rapid profiling of tetrabromobisphenol A bis(2-hydroxyethyl) ether [J]. | MICROCHEMICAL JOURNAL , 2025 , 216 . |
| MLA | Wu, Xinglin et al. "Dual-mode lateral flow immunoassay for rapid profiling of tetrabromobisphenol A bis(2-hydroxyethyl) ether" . | MICROCHEMICAL JOURNAL 216 (2025) . |
| APA | Wu, Xinglin , Wang, Leding , He, Youfen , Chen, Chengqun , Lin, Chenchen , Weng, Qibiao et al. Dual-mode lateral flow immunoassay for rapid profiling of tetrabromobisphenol A bis(2-hydroxyethyl) ether . | MICROCHEMICAL JOURNAL , 2025 , 216 . |
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Surface engineering of covalent organic frameworks (COFs) represents a highly promising strategy for achieving superior capture capacity towards environmental trace pollutants. Herein, the three-dimensional covalent organic frameworks (3D-COFs) with tunable crystallinity were synthesized and optimized via a simple solvent ratio adjustment, demonstrating an exceptional capture capacity of polychlorinated biphenyls (PCBs). By adjusting the ratio of solvents (1,4-dioxane and toluene), a functional 3D COF (dia-c5 TPA-TAM-COF) was discovered, providing an ideal approach to transform an amorphous compound into a functional platform with a significant increase in crystallinity, surface area and pore volume for PCBs capture. The adsorption features and analytical performance of PCBs were evaluated, and a good performance was achieved with high enrichment factors as 6973 ∼ 11,015. Optimization of solid phase microextraction (SPME) using the dia-c5 TPA-TAM-COF as the coating was investigated, and the method was verified for sensitive quantification of PCBs by using GC–MS, achieving the sensitive limits of detection (LODs) of 0.028 ∼ 0.0263 ng/L. Good reproducibility and robustness of the analytical performance of PCBs using dis-c5 was achieved, with the RSDs of intra-day (n = 3), inter-day (n = 6) and fiber-to-fiber (n = 3) of 0.7 ∼ 4.7 %, 1.8 ∼ 4.6 % and 2.0 ∼ 3.9 % respectively. The recoveries of PCBs in the soil fortified samples were obtained as 85.3 ∼ 101.9 % and 86.3 ∼ 108.8 %, respectively, as well as high thermal stability and long lifetime (keep on going more than 150 cycles). This study demonstrates that a simple protocol of adjusting the solvent content was capable of tailoring high crystallinity and suitable interpenetration degree of TPA-TAM-COFs for high performance target capture of PCBs in environmental soils. © 2025
Keyword ：
Carbon capture Carbon capture Environmental technology Environmental technology
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| GB/T 7714 | Chen, Qingai , Lu, Feifei , Chen, Chenqun et al. Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls [J]. | Journal of Chromatography A , 2025 , 1755 . |
| MLA | Chen, Qingai et al. "Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls" . | Journal of Chromatography A 1755 (2025) . |
| APA | Chen, Qingai , Lu, Feifei , Chen, Chenqun , Lin, Chenchen , wang, Jiabin , Lin, Xucong et al. Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls . | Journal of Chromatography A , 2025 , 1755 . |
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The occurrence of microcystins (MCs) during harmful algal blooms (HABs) represents a major threat to freshwater environments. In this work, a novel surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes (PCSs) was prepared for the enrichment of MCs. The copolymerization of bifunctional amphiphilic monomers, 2-methacryloyloxyethyl phosphorylcholine (MPC) and N-benzylquininium chloride (BQN), with the cross-linker methacryl substituted polyhedral oligomeric silsesquioxane (POSS) was achieved in an ionic liquid-based porogenic medium. The hierarchical porous structure, a variety of surface functional groups and weak hydrophilicity were well characterized on the prepared materials using scanning electron microscopy, nitrogen adsorption/desorption analysis, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, zeta potential analysis and water contact angle testing, respectively. The as-prepared surface amphiphilic PCSs was used as an adsorbent for pipette tip solid-phase extraction (PT-SPE) to enrich microcystins (MCs) from surface waters before their analysis by capillary electrochromatography (CEC) and liquid chromatography-mass spectrometry (LC-MS). Under the optimal conditions, the established PT-SPE-LC-MS method exhibited a wide linear range (10-10,000 ng L- 1), low limits of detection (4.0-8.0 ng L- 1) and satisfactory recoveries (89.5-102.8 %) for MCs. An adsorption mechanism involving electrostatic interactions, hydrogen bonding, hydrophilic interactions, and it-it stacking has been proposed. The findings suggest that the use of surface amphiphilic PCSs materials as adsorbents in the PT-SPE platform facilitates efficient enrichment of MCs for subsequent chromatographic analysis. These investigations offer a new perspective on the simple and uncomplicated pretreatment of complex environmental samples.
Keyword ：
Hybrid porous polymers based on cage-like Hybrid porous polymers based on cage-like Microcystins Microcystins organosiloxanes (PCSs) organosiloxanes (PCSs) Pipette tip solid phase extraction Pipette tip solid phase extraction Surface amphiphilic Surface amphiphilic
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| GB/T 7714 | Wang, Shuqiang , Liu, Wenning , Lei, Xiaoyun et al. Surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes for pipette tip solid-phase extraction of microcystins in water [J]. | JOURNAL OF CHROMATOGRAPHY A , 2024 , 1736 . |
| MLA | Wang, Shuqiang et al. "Surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes for pipette tip solid-phase extraction of microcystins in water" . | JOURNAL OF CHROMATOGRAPHY A 1736 (2024) . |
| APA | Wang, Shuqiang , Liu, Wenning , Lei, Xiaoyun , Huang, Ting , Huang, Guobin , Lin, Chenchen et al. Surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes for pipette tip solid-phase extraction of microcystins in water . | JOURNAL OF CHROMATOGRAPHY A , 2024 , 1736 . |
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Okadaic acid (OA) biotoxin acts a well-established inhibitor of protein phosphatase even a tumor promoter of human being, arouse great attention in safety monitoring. However, the powerful and convenient nanosensing technologies for addressing the demands such as rapidity, high sensitivity, and stability in the in-field test of OA shellfish toxin is still scarce. Herein, a high-performance magnetic biometric nanosensor (MBNS) integrating oriented aptamers and ultrasensitive laser-induced fluorescence (LIF) was firstly proposed for the in-field detection of trace OA in seafoods. High-density aptamers hybridized with FAM-labeled cDNA were tethered to the surface of AuNPs on magnetic MIL-101@Fe3O4, and then finely regulated by mercaptohexyl alcohol (MCH) to be orderly assembled, as was successfully utilized to engineer an active biological nanosensor for highly specific recognition of OA. Aptamers anchored on magnetic Fe3O4@MOF@AuNPs activate a biometric micro -reactor of OA, in which the superior LIF properties, conformation regulation of aptamer, and the specific recognition using aptamer genes were adopted. The magnetic nanosensor with an excellent specificity and super sensitivity for OA analysis was achieved within 20 min. Moreover, the content of captured OA could facilely be recorded by measuring the fluorescence intensity, and the limit of detection (LOD) and limit of quantitation of OA (LOQ) reached 0.015 and 0.050 ng/mL respectively, which was far better than most aptamer-based biometric sensing methods. The feasibility for accurate test of trace OA toxin in the fortified shellfish samples was validated with the recovery yields of 88.2-107.5% and RSD of 0.5-7.6%, respectively. The result demonstrated that the oriented-aptamer encoded MNS had significant practical values in rapid and ultrasensitive detection of OA biotoxin and the related safety applications.
Keyword ：
Laser-induced fluorescence Laser-induced fluorescence Magnetic nanosensor Magnetic nanosensor Okadaic acid Okadaic acid Oriented aptamer Oriented aptamer Ultrasensitive test Ultrasensitive test
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| GB/T 7714 | Zhou, Yuwei , Chen, Qingai , Huang, Guobin et al. Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid [J]. | TALANTA , 2024 , 266 . |
| MLA | Zhou, Yuwei et al. "Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid" . | TALANTA 266 (2024) . |
| APA | Zhou, Yuwei , Chen, Qingai , Huang, Guobin , Huang, Shukun , Lin, Chenchen , Lin, Xucong et al. Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid . | TALANTA , 2024 , 266 . |
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Accurate quantitation of trace-level ATP biomarkers often suffers from interference of sample matrix and remains a challenge. Herein, a novel magnetic biological nanosensor (MBNS) integrating tetrahedral framework nucleic acid (tFNA)-confined aptamers and ultrasensitive laser-induced fluorescence (LIF) was tailored and evaluated. The fluorescent aptamer nanoprobe hybridized with FAM-labelled cDNA was particularly incorporated into the inner cavity of tFNA, enabling a dual-mode mechanism that integrates molecular size selection and aptamer affinity recognition. By this strategy, the benefits of tFNA molecular sieve, the super sensitive properties of LIF, and the specific recognition of "aptamer gene codes" were integrated, as triggered the superior response of ATP. The structures, properties and reaction mechanism of tFNA, as well as tFNA modified with aptamer probe at different sites, were characterized in detail. A high level of anti-interference was achieved, shielding against nonspecific adsorption even in matrices with high protein and analogue content, 10(3)similar to 10(5) times higher than ATP. The release of fluorescent beacon from aptamer probe was facilely driven by specific binding of ATP, and an ultra-sensitive detection of 75 pmol/L ATP was obtained without amplification. It was 2-3 orders of magnitude more sensitive than most fluorescent aptasensors. Applied to serum samples, the stability and reproducibility of the resultant aptasensor have been validated with the intra- and inter-assay RSD in 1.6-6.4 % and -1.5-8.4 %, respectively. Acceptable recoveries of 96.5-106.8 % were achieved, consistent with the LC-MS method. It was expected to open a simple and efficient protocol for highly specific and ultrasensitive ATP detection in clinical diagnostics.
Keyword ：
Aptamer affinity Aptamer affinity Aptasensor Aptasensor Laser-induced fluorescence Laser-induced fluorescence Size selection Size selection Tetrahedral framework nucleic acid Tetrahedral framework nucleic acid
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| GB/T 7714 | Huang, Guobin , Xie, Qian , Chi, Jinxin et al. Magneto-fluorescent nanobiosensor with tetrahedral framework nucleic acid-confined aptamer for trace adenosine triphosphate detection [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 417 . |
| MLA | Huang, Guobin et al. "Magneto-fluorescent nanobiosensor with tetrahedral framework nucleic acid-confined aptamer for trace adenosine triphosphate detection" . | SENSORS AND ACTUATORS B-CHEMICAL 417 (2024) . |
| APA | Huang, Guobin , Xie, Qian , Chi, Jinxin , Lin, Chenchen , Lin, Xucong , Xie, Zenghong . Magneto-fluorescent nanobiosensor with tetrahedral framework nucleic acid-confined aptamer for trace adenosine triphosphate detection . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 417 . |
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