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学者姓名：林陈晨
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Abstract ：
Okadaic acid (OA) biotoxin acts a well-established inhibitor of protein phosphatase even a tumor promoter of human being, arouse great attention in safety monitoring. However, the powerful and convenient nanosensing technologies for addressing the demands such as rapidity, high sensitivity, and stability in the in-field test of OA shellfish toxin is still scarce. Herein, a high-performance magnetic biometric nanosensor (MBNS) integrating oriented aptamers and ultrasensitive laser-induced fluorescence (LIF) was firstly proposed for the in-field detection of trace OA in seafoods. High-density aptamers hybridized with FAM-labeled cDNA were tethered to the surface of AuNPs on magnetic MIL-101@Fe3O4, and then finely regulated by mercaptohexyl alcohol (MCH) to be orderly assembled, as was successfully utilized to engineer an active biological nanosensor for highly specific recognition of OA. Aptamers anchored on magnetic Fe3O4@MOF@AuNPs activate a biometric micro -reactor of OA, in which the superior LIF properties, conformation regulation of aptamer, and the specific recognition using aptamer genes were adopted. The magnetic nanosensor with an excellent specificity and super sensitivity for OA analysis was achieved within 20 min. Moreover, the content of captured OA could facilely be recorded by measuring the fluorescence intensity, and the limit of detection (LOD) and limit of quantitation of OA (LOQ) reached 0.015 and 0.050 ng/mL respectively, which was far better than most aptamer-based biometric sensing methods. The feasibility for accurate test of trace OA toxin in the fortified shellfish samples was validated with the recovery yields of 88.2-107.5% and RSD of 0.5-7.6%, respectively. The result demonstrated that the oriented-aptamer encoded MNS had significant practical values in rapid and ultrasensitive detection of OA biotoxin and the related safety applications.
Keyword ：
Laser-induced fluorescence Laser-induced fluorescence Magnetic nanosensor Magnetic nanosensor Okadaic acid Okadaic acid Oriented aptamer Oriented aptamer Ultrasensitive test Ultrasensitive test
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| GB/T 7714 | Zhou, Yuwei , Chen, Qingai , Huang, Guobin et al. Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid [J]. | TALANTA , 2024 , 266 . |
| MLA | Zhou, Yuwei et al. "Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid" . | TALANTA 266 (2024) . |
| APA | Zhou, Yuwei , Chen, Qingai , Huang, Guobin , Huang, Shukun , Lin, Chenchen , Lin, Xucong et al. Oriented-aptamer encoded magnetic nanosensor with laser-induced fluorescence for ultrasensitive test of okadaic acid . | TALANTA , 2024 , 266 . |
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本实用新型涉及一种用于海水总氮检测的装置，包括试剂供应模块、流动注射控制模块、消解反应模块、还原反应/比色检测模块和废液收集模块，本实用新型基于氧化消解、钒试剂还原和偶氮比色反应，待测海水样品依次在消解反应模块和还原/比色检测模块中连续进行海水含氮物质的紫外光‑热协同催化过硫酸盐氧化消解、硝酸盐还原反应和亚硝酸盐显色反应，实现海水总氮的测定，仪器避免了使用高温高压消解反应装置和镉金属还原柱，实验条件较为温和，反应速度快，可应用于海水中总氮的检测分析。
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| GB/T 7714 | 林旭聪 , 刘影 , 林陈晨 . 一种用于海水总氮检测的装置 : CN202321410118.2[P]. | 2023-06-05 00:00:00 . |
| MLA | 林旭聪 et al. "一种用于海水总氮检测的装置" : CN202321410118.2. | 2023-06-05 00:00:00 . |
| APA | 林旭聪 , 刘影 , 林陈晨 . 一种用于海水总氮检测的装置 : CN202321410118.2. | 2023-06-05 00:00:00 . |
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Efficient fabrication of excellent fiber coatings for high-performance solid-phase microextraction (SPME) is interesting. Here, a high-efficiency synthesis strategy on graphene oxide/polyhedral oligomeric silsesquioxane (GO/POSS) composite-coated fiber was exploited via an ultra-fast UV polymerization in one step, and applied to excellent SPME of polycyclic musks (PCMs). Using methacryloxy siloxane-grafted GO and POSS-methacryl substituted (POSS-MA) as functional monomers, a facile and direct UV polymerization of multiple reactions including alkenyl radical reaction and thiol-ene click chemistry was fulfilled on thiol-pretreated fiber in only 5 min without tedious process. Characteristics such as morphology, FT-IR, XPS, BET and TG of fiber coating were studied in detail, as well as the SPME performance. Attributing to the rigid stereo conformation of POSS and large conjugate plane of GO-based nanosheet, the significant surface area, high hydrophobicity and intrinsic 7C-7C in-teractions were adopted in fiber coating. In comparison of commercial SPME fibers, POSS-based or GO-based SPME fibers, a superior extraction performance towards PCMs was achieved with GO/POSS-coated fiber. High-efficiency extraction of PCMs was gained with prominent enrichment factors and the sensitive detection limits of PCMs were of 0.04-0.12 ng/L. In particular, the extraction efficiency was robust and still maintained a high level above 93% for PCMs even after 150 cycle's applications. Good recoveries of PCMs reached 86.6%- 102.1% and 86.7%-101.3% in river water and cosmetic samples, respectively. It lights an attractive approach to efficiently fabricating robust GO/POSS-coated fiber for high-performance SPME of PCMs.
Keyword ：
Graphene oxide Graphene oxide One-step polymerization One-step polymerization Polycyclic musks Polycyclic musks Polyhedral oligomeric silsesquioxane Polyhedral oligomeric silsesquioxane Solid-phase microextraction Solid-phase microextraction
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| GB/T 7714 | Jiang, Shuangchen , Wan, Shuangfeng , Du, Hong et al. Facile one-step synthesis of graphene oxide/polyhedral oligomeric silsesquioxane composite-coated fiber for high-efficiency solid-phase microextraction of polycyclic musks [J]. | ANALYTICA CHIMICA ACTA , 2022 , 1234 . |
| MLA | Jiang, Shuangchen et al. "Facile one-step synthesis of graphene oxide/polyhedral oligomeric silsesquioxane composite-coated fiber for high-efficiency solid-phase microextraction of polycyclic musks" . | ANALYTICA CHIMICA ACTA 1234 (2022) . |
| APA | Jiang, Shuangchen , Wan, Shuangfeng , Du, Hong , Lin, Chenchen , Lin, Xucong . Facile one-step synthesis of graphene oxide/polyhedral oligomeric silsesquioxane composite-coated fiber for high-efficiency solid-phase microextraction of polycyclic musks . | ANALYTICA CHIMICA ACTA , 2022 , 1234 . |
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A novel aptamer-functionalized metal-organic framework nanofibrous composite (viz. PAN/UiO@UiO(2)-N-3-aptamer) with a high aptamer coverage density was proposed based on the electrospinning and seeded growth method, and used for specific affinity recognition of trace Microcystin-LR (MC-LR). Heterobifunctional ligand was used to modify the metal-organic framework nanoparticles (MOF NPs) surface, which could passivate the MOF surface with respect to unmodified DNA, followed by coupling massive aptamers on MOF of the solid-phase microextraction (SPME) fiber using click chemistry. Characterizations including morphology, spectra analysis, mechanical stability, binding capacity and specificity were fulfilled. Applied to the analysis of MC-LR, the good selective and sensitive recognition were obtained with the detection limit as low as 0.003 ng/mL, which was better than most non-specific SPME or solid-phase extraction (SPE) protocols. The stability and reproducibility were acceptable, and the intra-day, inter-day and column-to-column relative standard deviations (RSDs) for the recovery of MC-LR were gained in the range from 2.5% to 14.3%, respectively. Satisfactory recoveries of MC-LR in environmental water samples were measured as 96.3 +/- 4.7% - 98.9 +/- 2.7% (n = 3) in tap water, 94.4 +/- 2.5% 96.1 +/- 3.5% (n = 3) in pond water, and 97.0 +/- 2.1% - 97.9 +/- 3.1% (n = 3) in river water, respectively. This work demonstrated that the electrospun nanofibrous composite with massive aptamers would be a better alternative for ultra-trace MC-LR detection with good selectivity, matrix-resistance ability and high resolution.
Keyword ：
Aptamer Aptamer Click chemistry Click chemistry Electrospinning Electrospinning Microcystin-LR Microcystin-LR Selective extraction Selective extraction
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| GB/T 7714 | Xu, Zhiqun , Zhang, Zhexiang , She, Zongkang et al. Aptamer-functionalized metal-organic framework-coated nanofibers with multi-affinity sites for highly sensitive, selective recognition of ultra-trace microcystin-LR [J]. | TALANTA , 2022 , 236 . |
| MLA | Xu, Zhiqun et al. "Aptamer-functionalized metal-organic framework-coated nanofibers with multi-affinity sites for highly sensitive, selective recognition of ultra-trace microcystin-LR" . | TALANTA 236 (2022) . |
| APA | Xu, Zhiqun , Zhang, Zhexiang , She, Zongkang , Lin, Chenchen , Lin, Xucong , Xie, Zenghong . Aptamer-functionalized metal-organic framework-coated nanofibers with multi-affinity sites for highly sensitive, selective recognition of ultra-trace microcystin-LR . | TALANTA , 2022 , 236 . |
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Developing functional fiber coating for selective solid phase microextraction (SPME) of trace pollutants is critical in environmental analysis. Herein, the novel covalent organic frameworks (COFs) with three-dimensional (3D) frameworks and multiple interactions were designed and presented for the selec-tive SPME of polychlorinated biphenyls (PCBs). Using tetra (p-aminophenyl) methane (TAM) and 1,3,5-triformylphloroglucinol (Tp) as the monomers, the 3D TpTAM-COF was synthesized and possessed a large specific surface area, high thermal stability, and spatial selectivity toward PCBs. Characterizations such as morphology, XPS, XRD, thermal stability, and enhancement factors (EFs) were studied. Multiple in-teractions including pi-pi conjugation, hydrophobic interaction, and selectivity toward non-planar struc-ture were adopted, which resulted in a superior adsorption affinity toward PCBs on TpTAM-COF. Under the optimal conditions, the spatial selectivity toward PCBs, organic analogs (o-dichlorobenzene, biphenyl) and polycyclic aromatic hydrocarbons (naphthalene, pyrene, and anthracene)) was achieved. Efficient and selective adsorption of fifteen PCBs was fulfilled with the highest EF up to 10305. Using the HS-SPME-GC-MS method, the recoveries of PCBs in the river water and soil samples were determined to be 84.8 +/- 7.8% -117.2 +/- 8.5% (n = 3) and 84.4 +/- 8.6% -114.7 +/- 7.6% (n = 3), respectively. Compared with most commercial SPME fibers and other COFs-based fibers, the resultant TpTAM-COF-coated fibers possessed higher selectivity and EFs of PCBs. It proposed a promising approach for selective SPME of trace PCBs by multiple interactions in the steric structure of 3D COFs.(c) 2022 Elsevier B.V. All rights reserved.
Keyword ：
3D covalent organic framework 3D covalent organic framework Fiber coating Fiber coating Polychlorinated biphenyls Polychlorinated biphenyls Selective solid phase microextraction Selective solid phase microextraction
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| GB/T 7714 | Lu, Feifei , Wu, Mengqin , Lin, Chenchen et al. Efficient and selective solid-phase microextraction of polychlorinated biphenyls by using a three-dimensional covalent organic framework as functional coating [J]. | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1681 . |
| MLA | Lu, Feifei et al. "Efficient and selective solid-phase microextraction of polychlorinated biphenyls by using a three-dimensional covalent organic framework as functional coating" . | JOURNAL OF CHROMATOGRAPHY A 1681 (2022) . |
| APA | Lu, Feifei , Wu, Mengqin , Lin, Chenchen , Lin, Xucong , Xie, Zenghong . Efficient and selective solid-phase microextraction of polychlorinated biphenyls by using a three-dimensional covalent organic framework as functional coating . | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1681 . |
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Fabricating functional electrospun nanofiber coating for highly selective extraction of microcystin-LR (MC-LR) was of significant importance for water-safety monitoring. Herein, a novel MOF@aptamer functional-ized nanofabric was presented via a facile and reliable strategy integrating polydopamine (PDA) mediation and thiol-ene chemistry and applied for specific recognition of the MC-LR model analyte. Using poly -dopamine (PDA) as the mediating layer, vinyl-UiO-66 MOF was grown in situ, followed by post-synthetic modification (PSM) of Zr4 + with vinyl phosphate and rapid UV-initiated click reaction of aptamers. Uni-form deposition of Zr-based MOF (vinyl-UiO-66) on the nanofibers was directly produced, and the tedious co-electrospinning process was abandoned to prevent the aggregation and encapsulation of MOF. Via an efficient "thiol-ene" chemistry, massive thiol-terminated aptamers were grafted on MOF within one step under friendly conditions, rather than the time-consuming nanoparticle adsorption or unfriendly cova-lent chemical reactions. As a result, the robust MOF@aptamer-coated nano-fabrics were obtained, and a highly selective performance towards MC-LR was illustrated with a limit of detection (LOD) at 0.002 ng/mL, good precision (CV < 8.3%), good repeatability (2.2-6.0%) when coupled with LC-MS. Almost 1-2 orders of magnitude higher detection sensitivity was exhibited than that of the common non-specific SPE/SPME fiber reported so far. Applied to water samples, the good matrix-resistance ability, and accept-able recovery yields were achieved with high specificity. This strategy might provide a rapid and friendly protocol to efficiently fabricate MOF@aptamer functionalized nano-fabrics through electrospinning and interfacial "thiol-ene" chemistry for highly-selective microextraction.(c) 2022 Elsevier B.V. All rights reserved.
Keyword ：
Aptamer Aptamer Electrospun nanofiber Electrospun nanofiber Metal -organic framework Metal -organic framework Polydopamine Polydopamine Thiol-ene chemistry Thiol-ene chemistry
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| GB/T 7714 | Xie, Qian , Xu, Zhiqun , Huang, Guobin et al. Bioinspired polydopamine-mediated metal-organic framework click-grafting aptamers functionalized fabric for highly-specific recognition of microcystin-leucine arginine [J]. | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1688 . |
| MLA | Xie, Qian et al. "Bioinspired polydopamine-mediated metal-organic framework click-grafting aptamers functionalized fabric for highly-specific recognition of microcystin-leucine arginine" . | JOURNAL OF CHROMATOGRAPHY A 1688 (2022) . |
| APA | Xie, Qian , Xu, Zhiqun , Huang, Guobin , Lin, Chenchen , Lin, Xucong . Bioinspired polydopamine-mediated metal-organic framework click-grafting aptamers functionalized fabric for highly-specific recognition of microcystin-leucine arginine . | JOURNAL OF CHROMATOGRAPHY A , 2022 , 1688 . |
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A novel polyhedral oligomeric siloxane (POSS)-based zwitterionic monolithic capillary column was prepared via one-pot polymerization in ionic liquid porogen, using N,N-dimethyl-N-methacryloxyethyl-N-(3sulfopropyl)ammonium betaine (DMMSA) and methacrylic ethyl trimethylammonium chloride (META) as binary functional monomers, and methacryl substituted POSS as cross-linker. The pore structure, permeability and homogeneity were well tuned by optimizing the polymerization conditions. The resultant monolith was characterized by scanning electron microscopy, nitrogen adsorption/desorption isotherm and Fourier transform infrared spectroscopy. The incorporation of zwitterionic ligand (DMMSA), quaternary amine group (META) and rigid POSS skeleton endows the hybrid organic-silica monolith with high hydrophilicity, electrostatic interaction and good mechanical stability, as well as a tunable electroosmotic flow over wide pH range. A close investigation of capillary electrochromatographic separations of different types of polar compounds such as bases, nucleosides and benzoic acids on such stationary phase exhibited a retention independent column efficiency up to 118,0 0 0 plates/m (thiourea), as well as a mixed-mode hydrophilic interaction chromatography (HILIC) retention mechanism including weak electrostatic interaction, hydrophobic interaction and anion exchange. (c) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Capillary electrochromatography Capillary electrochromatography Ionic liquid Ionic liquid Mixed-mode HILIC retention Mixed-mode HILIC retention POSS POSS Zwitterionic monolithic column Zwitterionic monolithic column
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| GB/T 7714 | Huang, Ting , Zhang, Wanru , Lei, Xiaoyun et al. Rapid polymerization of polyhedral oligomeric siloxane-based zwitterionic sulfoalkylbetaine monolithic column in ionic liquid for hydrophilic interaction capillary electrochromatography [J]. | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1659 . |
| MLA | Huang, Ting et al. "Rapid polymerization of polyhedral oligomeric siloxane-based zwitterionic sulfoalkylbetaine monolithic column in ionic liquid for hydrophilic interaction capillary electrochromatography" . | JOURNAL OF CHROMATOGRAPHY A 1659 (2021) . |
| APA | Huang, Ting , Zhang, Wanru , Lei, Xiaoyun , Chen, Huijuan , Lin, Chenchen , Wu, Xiaoping . Rapid polymerization of polyhedral oligomeric siloxane-based zwitterionic sulfoalkylbetaine monolithic column in ionic liquid for hydrophilic interaction capillary electrochromatography . | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1659 . |
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Time-consuming or tedious operation in multiple-step process might is the obstacle for efficiently preparing aptamer-affinity monolithic column. Here, a new and facile strategy to prepare aptamer-based hybrid affinity monolith in "one-pot" at room temperature was exploited, in which UV light-initiated free-radical polymerization and "thiol-ene" click reaction were implemented simultaneously. Only 7 min was cost for finishing the polymerization reaction, which was only 1/100 of that for the traditional thermal polymerization. Using ochratoxin A (OTA) as the model analyte, the recipe for photo-initiated polymerization was optimized, and SEM morphology, FTIR, EDS, pore size distribution and specific recognition performance were also studied. Compared with traditional thermal polymerization, the resultant monolith was achieved more facilely and displayed better results such as more homogeneous skeleton structure, higher reaction efficiency of aptamer (>88.2%) and better specific selectivity to OTA. Besides, an extremely low nonspecific adsorption of analogues was obtained and showed a level at only 1/25 of that in the similar aptamer-affinity monolith prepared by thermal polymerization. Applied to beer and red wine samples, good recovery yields about 99.7 +/- 4.0% e101.2 +/- 2.3% (n = 3)was achieved with the acceptable RSDs. It would open up a rapid and promising access to efficiently preparing highperformance aptamer-based affinity monolithic columns for online specific recognition. (c) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Affinity monolith Affinity monolith Aptamer Aptamer Light-initiated polymerization Light-initiated polymerization One-pot One-pot Online specific recognition Online specific recognition
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| GB/T 7714 | Zhao, Tingting , Ding, Xinyue , Chen, Yiqiong et al. Towards highly specific aptamer-affinity monolithic column by efficient UV light-initiated polymerization in "one-pot" [J]. | ANALYTICA CHIMICA ACTA , 2021 , 1165 . |
| MLA | Zhao, Tingting et al. "Towards highly specific aptamer-affinity monolithic column by efficient UV light-initiated polymerization in "one-pot"" . | ANALYTICA CHIMICA ACTA 1165 (2021) . |
| APA | Zhao, Tingting , Ding, Xinyue , Chen, Yiqiong , Lin, Chenchen , Qi, Guomin , Lin, Xucong et al. Towards highly specific aptamer-affinity monolithic column by efficient UV light-initiated polymerization in "one-pot" . | ANALYTICA CHIMICA ACTA , 2021 , 1165 . |
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A novel aptamer@AuNPs@UiO-66-NH2 electrospun nanofibrous coating fiber for specific recognition of microcystin-LR (MC-LR) was proposed by using electrospinning, metal-organic frameworks (MOF) seed growth and AuNPs bridging aptamer strategies. Characterization of morphology, structure and stability of the obtained affinity nanofibrous coating fiber were investigated. High loading of MOFs and aptamers on the nanofibrous fiber were achieved and successfully applied for accurate identification of MC-LR by solid-phase microextraction (SPME) coupled with LC-MS. Highly specific recognition of MC-LR with little interference of analogs was achieved with extremely low LOD (0.004 ng/mL), good precision (CV% < 11.0%) and low relative error (RE% = -1.5% to -10.0%), which was rather better than that of the traditional SPME or SPE protocols. Satisfactory recoveries of MC-LR were obtained in the range of 92.0-96.8% (n = 3) in fortified tap water, raw pond water and river water samples. This work revealed an attractive alternative access to specific recognition and super-sensitive analysis of MC-LR in water. (C) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Aptamer Aptamer Au nanoparticles Au nanoparticles Electrospun nanofibrous composite Electrospun nanofibrous composite Microcystin Microcystin Specific recognition Specific recognition
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| GB/T 7714 | Xu, Zhiqun , Fan, Guanghui , Zheng, Tuo et al. Aptamer-functionalized metal-organic framework-based electrospun nanofibrous composite coating fiber for specific recognition of ultratrace microcystin in water [J]. | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1656 . |
| MLA | Xu, Zhiqun et al. "Aptamer-functionalized metal-organic framework-based electrospun nanofibrous composite coating fiber for specific recognition of ultratrace microcystin in water" . | JOURNAL OF CHROMATOGRAPHY A 1656 (2021) . |
| APA | Xu, Zhiqun , Fan, Guanghui , Zheng, Tuo , Lin, Chenchen , Lin, Xucong , Xie, Zenghong . Aptamer-functionalized metal-organic framework-based electrospun nanofibrous composite coating fiber for specific recognition of ultratrace microcystin in water . | JOURNAL OF CHROMATOGRAPHY A , 2021 , 1656 . |
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Developing a functional affinity monolithic column towards in-tube solid-phase microextraction (IT-SPME) for selective sample pretreatment is critical. Herein, a high-performance capillary affinity monolithic column with an ultra-high aptamer coverage density was rapidly fabricated via a simple adsorption strategy, in which aptamers with natural sequences were directly immobilized on an ammonium-based strongly cationic matrix. Limitations of the traditional biological or covalent methods such as time-consuming modification reactions, special requirement of active groups (e.g. -NH2 and -SH) on the aptamer, and low aptamer coverage density levels were avoided. An ultra-high coverage density of 8616 pmol mu L-1 was achieved with excellent stability, and the highest aptamer-modification level among all the current methods was reached. Selective recognition and high recovery yields of the model mycotoxin ochratoxin A (OTA) were achieved in 95.9 +/- 0.98%-97.9 +/- 0.28% (n = 3). In particular, there was little cross-reactivity towards the OTB analogue of only 0.5% even in the case of 250 fold of the analogue OTB, which was not reported in previous affinity monoliths. Upon sample analysis, satisfactory discriminations of trace OTA were obtained at 93.7 +/- 1.4%-95.5 +/- 2.5% (n = 3) in beer and wheat. A facile and direct method for efficiently fabricating an aptamer-based affinity monolith towards online selective IT-SPME was proposed.
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| GB/T 7714 | Zhao, Tingting , Tong, Shiqian , Zhou, Susu et al. A facile aptamer immobilization strategy to fabricate a robust affinity monolith for highly specific in-tube solid-phase microextraction [J]. | ANALYST , 2021 , 146 (18) : 5732-5739 . |
| MLA | Zhao, Tingting et al. "A facile aptamer immobilization strategy to fabricate a robust affinity monolith for highly specific in-tube solid-phase microextraction" . | ANALYST 146 . 18 (2021) : 5732-5739 . |
| APA | Zhao, Tingting , Tong, Shiqian , Zhou, Susu , Lin, Chenchen , Lin, Xucong , Xie, Zenghong . A facile aptamer immobilization strategy to fabricate a robust affinity monolith for highly specific in-tube solid-phase microextraction . | ANALYST , 2021 , 146 (18) , 5732-5739 . |
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