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学者姓名：刘乐冕
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Abstract ：
Polyethylene terephthalate (PET) is a polymer synthesized via the dehydration and condensation reaction between ethylene glycol and terephthalic acid. PET has emerged as one of the most extensively employed plastic materials due to its exceptional plasticity and durability. Nevertheless, PET has a complex structure and is extremely difficult to degrade in nature, causing severe pollution to the global ecological environment and posing a threat to human health. Currently, the methods for PET processing mainly include physical, chemical, and biological methods. Biological enzyme degradation is considered the most promising PET degradation method. In recent years, an increasing number of enzymes that can degrade PET have been identified, and they primarily target the ester bond of PET. This review comprehensively introduced the latest research progress in PET enzymatic degradation from the aspects of PET-degrading enzymes, PET biodegradation pathways, the catalytic mechanism of PET-degrading enzymes, and biotechnological strategies for enhancing PET-degrading enzymes. On this basis, the current challenges within the enzymatic PET degradation process were summarized, and the directions that need to be worked on in the future were pointed out. This review provides a reference and basis for the subsequent effective research on PET biodegradation.
Keyword ：
Biodegradation pathway Biodegradation pathway Bioengineering modification Bioengineering modification Catalytic mechanism Catalytic mechanism PET-degrading enzyme PET-degrading enzyme
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| GB/T 7714 | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate [J]. | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
| MLA | Qiu, Jiarong et al. "A comprehensive review on enzymatic biodegradation of polyethylene terephthalate" . | ENVIRONMENTAL RESEARCH 240 (2024) . |
| APA | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing , Wu, Jinzhi , Zeng, Xianhai , Shi, Xinguo et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate . | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
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Combination of taxa and function can provide a more comprehensive picture on human-induced microbial homogenization. Here, we obtained 2.58 billion high-throughput sequencing reads and 479 high-quality metagenome-assembled genomes (MAGs) of planktonic microbial communities in a subtropical river for 5 years. We found the microbial taxa homogenization and functional homogenization were uncoupled. Although human activities in downstream sites significantly decreased the taxonomic diversity of non-abundant ASV communities (16S rRNA gene amplicon sequence variants), they did not significantly decrease the taxonomic diversity of abundant ASV and total observed MAG communities. However, the total observed MAG communities in downstream sites tended to homogenize into some specific taxa which encode human-activity-related functional genes, such as nutrient cycles, greenhouse gas emission, antibiotic and arsenic resistance. Those specific MAGs with high taxonomic diversity caused the weak heterogenization of total observed MAG communities in downstream sites. Moreover, functional homogenization promoted the synchrony among downstream MAGs, and these MAGs constructed some specific network modules might to synergistically execute or resist the humanactivity-related functions. High synchrony also led to the tandem effects among MAGs and thus decreased community stability. Overall, our findings revealed the links of microbial taxa, functions and stability under human activity impacts, and provided a strong evidence to encourage us re-thinking biotic homogenization based on microbial taxa and their functional attributes.
Keyword ：
Biotic homogenization Biotic homogenization Human activities Human activities Metagenome Metagenome Microbial community Microbial community Nitrogen cycle Nitrogen cycle
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| GB/T 7714 | Liu, Lemian , Wang, Hongwei , Guo, Yisong et al. Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability [J]. | WATER RESEARCH , 2024 , 252 . |
| MLA | Liu, Lemian et al. "Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability" . | WATER RESEARCH 252 (2024) . |
| APA | Liu, Lemian , Wang, Hongwei , Guo, Yisong , Yan, Qi , Chen, Jianfeng . Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability . | WATER RESEARCH , 2024 , 252 . |
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How microbial communities respond to wastewater fluctuations is poorly understood. Full-scale surface flow constructed wetlands (SFCWs) were constructed for investigating microbial communities. Results showed that influent wastewater changed sediment bacterial community composition seasonally, indicating that a single bacterial taxonomic group had low resistance (especially, Actinobacteriota and Gammaproteobacteria). However, copy numbers of 16S rRNA, ammonia oxidizing archaea, ammonia oxidizing bacteria, nirS and nirK in the first stage SFCWs were 2.49 x 1010, 3.48 x 109, 5.76 x 106, 8.77 x 108 and 9.06 x 108 g-1 dry sediment, respectively, which remained stable between seasons. Moreover, decreases in the nitrogen concentration in wastewater, changed microbial system state from heterotrophic to autotrophic. Micro-eukaryotic communities were more sensitive to wastewater fluctuations than bacterial communities. Overall, results revealed that microbial communities responded to spatio-temporal fluctuations in wastewater through state changes and species asynchrony. This highlighted complex processes of wastewater treatment by microbial components in SFCWs.
Keyword ：
Asynchrony Asynchrony Compensatory dynamics Compensatory dynamics Microbial community stability Microbial community stability Nitrogen removal Nitrogen removal Substrate Substrate
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| GB/T 7714 | Li, Xi , Xia, Menghua , Liu, Lemian et al. Response of bacterial and micro-eukaryotic communities to spatio-temporal fluctuations of wastewater in full scale constructed wetlands [J]. | BIORESOURCE TECHNOLOGY , 2024 , 399 . |
| MLA | Li, Xi et al. "Response of bacterial and micro-eukaryotic communities to spatio-temporal fluctuations of wastewater in full scale constructed wetlands" . | BIORESOURCE TECHNOLOGY 399 (2024) . |
| APA | Li, Xi , Xia, Menghua , Liu, Lemian , Li, Yuyuan , Wu, Jinshui . Response of bacterial and micro-eukaryotic communities to spatio-temporal fluctuations of wastewater in full scale constructed wetlands . | BIORESOURCE TECHNOLOGY , 2024 , 399 . |
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Dinoflagellate blooms have negative adverse effects on marine ecosystems. However, our knowledge about the spatiotemporal distribution of dinoflagellate communities and their correlations with micro-eukaryotic and bacterial communities is still rare. Here, the sediment micro-eukaryotic and bacterial communities were explored in the Taiwan Strait (TWS) by 16S and 18S rRNA gene high-throughput sequencing. We found that the dinoflagellates were the most abundant algal group in TWS, and their relative abundance was higher in spring and autumn than in summer. Moreover, the species richness and community composition of dinoflagellates showed strong seasonal patterns. NO3-N and NH4-N had the strongest correlations with the spatiotemporal dynamics of community composition of dinoflagellates. The dinoflagellates had a significantly wider niche breadth than other algal groups for NH4-N, NO3-N and NO2-N, and therefore potentially contributed to a wider distribution range and high abundance in TWS. In addition, the dinoflagellates had stronger impacts on microeukaryotes than on bacteria for both community composition and species richness. However, the dinoflagellates showed close coexistence with bacteria but loose coexistence with microeukaryotes in spring co-occurrence networks. This close coexistence suggests the potentially strong synergy effects between dinoflagellates and bacteria in spring dinoflagellate blooms in TWS. Overall, this study revealed the distribution mechanisms of dinoflagellates in TWS based on niche breadth and also unveiled the different effects of dinoflagellates on micro-eukaryotic and bacterial communities.
Keyword ：
Bacterial communities Bacterial communities Dinoflagellate Dinoflagellate Harmful algal bloom Harmful algal bloom Micro-eukaryotic community Micro-eukaryotic community Niche breadth Niche breadth
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| GB/T 7714 | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe et al. Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities [J]. | MARINE POLLUTION BULLETIN , 2024 , 208 . |
| MLA | Wang, Hongwei et al. "Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities" . | MARINE POLLUTION BULLETIN 208 (2024) . |
| APA | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe , Chen, Jianfeng . Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities . | MARINE POLLUTION BULLETIN , 2024 , 208 . |
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[目的]探明肥料种类和施肥量对秋茄(Kandelia obovata)幼苗生长的影响以及土壤微生物的响应.[方法]选用 3种有机肥和 1种无机肥,以不施肥组(CK)为对照,设置了 4个施肥量梯度,测定秋茄幼苗株高、叶长、叶宽、叶片数增长量、叶绿素含量,并对土壤微生物群落进行 16S rRNA基因高通量测序.[结果]秋茄最适施肥条件是 9.74 g·kg-1 基质的尊龙牌蚓肥,120 d后株高、叶长、叶宽、叶数增长量比对照组分别增加 117.50%、51.15%、63.34%、178.57%.施有机肥、无机肥和对照组的土壤微生物群落组成差异明显,施有机肥土壤中有更多特有的微生物.施有机肥土壤微生物α多样性变化趋势与植物生长指标变化趋势正相关,而施无机肥的呈负相关.表明有机肥可能通过促进土壤微生物与植物协同的方式进而有助于植物生长.施有机肥提高了土壤潜在有益菌,如放线菌门(Actinobacteriota)和Nitrospira、Nocardioides、Limibaculum属丰度.[结论]施适量有机肥协同促进秋茄生长和土壤微生物多样性,而施无机肥对秋茄生长促进作用相对较小,并对土壤微生物多样性产生负面影响.
Keyword ：
土壤微生物群落 土壤微生物群落 施肥条件 施肥条件 秋茄 秋茄 红树植物 红树植物
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| GB/T 7714 | 李龙 , 张梅玲 , 刘乐冕 et al. 不同施肥条件对秋茄幼苗培育及土壤微生物的影响 [J]. | 福建农业学报 , 2024 , 39 (7) : 839-847 . |
| MLA | 李龙 et al. "不同施肥条件对秋茄幼苗培育及土壤微生物的影响" . | 福建农业学报 39 . 7 (2024) : 839-847 . |
| APA | 李龙 , 张梅玲 , 刘乐冕 , 郭毅松 , 陈剑锋 . 不同施肥条件对秋茄幼苗培育及土壤微生物的影响 . | 福建农业学报 , 2024 , 39 (7) , 839-847 . |
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Cyanobacterial blooms have profound effects on the structure and function of plankton communities in inland waters, but few studies have focused on the effects of microbial-based processes in one-carbon and nitrogen cycling on water quality improvement following the bloom. Here, we compared the structure and function of the bacterial community, focusing on microbial one-carbon and nitrogen metabolisms during and after a cyanobacterial Microcystis bloom in a deep subtropical reservoir. Our data showed that microbial one-carbon and nitrogen cycles were closely related to dif-ferent periods of the bloom, and the changes of functional genes in microbial carbon and nitrogen cycling showed the same consistent trend as that of Methylomonas sp. With the receding of the bloom, the abundance of Methylomonas as well as the functional genes of microbial one-carbon and nitrogen cycling reached the peak and then recovered. Our results indicate that microbial one-carbon and nitrogen metabolisms were beneficial to the recovery of water quality from the cyanobacterial bloom. This study lays a foundation for a deep understanding of the cyanobacterial decompo-sition mediated by microbes in one-carbon and nitrogen cycles in inland freshwaters.
Keyword ：
Cyanobacterial bloom Cyanobacterial bloom Methanotrophs Methanotrophs Nitrogen cycle Nitrogen cycle One-carbon cycle One-carbon cycle Subtropical reservoir Subtropical reservoir
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| GB/T 7714 | Yu, Zheng , Peng, Xuan , Liu, Lemian et al. Microbial one-carbon and nitrogen metabolisms are beneficial to the reservoir recovery after cyanobacterial bloom [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 856 . |
| MLA | Yu, Zheng et al. "Microbial one-carbon and nitrogen metabolisms are beneficial to the reservoir recovery after cyanobacterial bloom" . | SCIENCE OF THE TOTAL ENVIRONMENT 856 (2023) . |
| APA | Yu, Zheng , Peng, Xuan , Liu, Lemian , Yang, Jun R. , Zhai, Xingyu , Xue, Yuanyuan et al. Microbial one-carbon and nitrogen metabolisms are beneficial to the reservoir recovery after cyanobacterial bloom . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 856 . |
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Aim: The geographical distribution of bacteria is an important, but poorly understood, topic in microbial ecology. A major question is how broadly distributed generalist taxa, and limitedly distributed specialist taxa, vary across a latitudinal gradient in freshwater ecosystems. We predict that: (a) generalists and specialists exhibit latitudinal diversity gradient with different patterns; (b) their community assemblies are mainly driven by stochastic processes; and (c) generalists coexist through niche differences, while specialists coexist through fitness differences. Location: Sixty lakes and reservoirs in China, ranging from 18 degrees N to 50 degrees N. ime Period: July-August in 2012 and 2018. Taxa Studied: Bacterioplankton. Methods: We clustered zero-radius operational taxonomic unit (zOTU) (subspecies-like level) into operational taxonomic unit (OTU) (species-like level) and calculated the intra-specific variation and spatial asynchrony of OTU. We examined the relative effects of environment versus space (latitude) on bacterial community and quantified the community assembly processes of generalists and specialists, respectively. The binary-state speciation and extinction (BiSSE) model has been used to estimate the evolutionary characteristics. The abundances of the top zOTUs within each OTU were identified to reveal their niche preferences (distribution patterns at different sites). Results: We found that bacterial generalists had a higher intra-specific variation, greater asynchrony and wider niche width than the specialists. With increasing latitude, bacterial diversity decreased with abundance variations in generalists and occurrence variations in specialists, respectively. Diversification in stochastic processes was the main driving factor in both groups, though deterministic processes showed greater effects on specialists than generalists in local communities. Bacterial generalists coexisted through niche differences, whereas specialists coexisted through average fitness differences. Main Conclusions: We demonstrated distinct latitudinal distribution patterns of bacterial generalists and specialists. These results highlight differences in biogeography of microbial generalist and specialist taxa mediated by intra-specific variation and emphasize their distinctly vital roles mediated by differing mechanisms in freshwater ecosystems.
Keyword ：
bacterial diversity bacterial diversity community assembly community assembly community ecology community ecology intra-specific variation intra-specific variation plankton plankton species coexistence species coexistence
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| GB/T 7714 | Zuo, Jun , Liu, Lemian , Xiao, Peng et al. Patterns of bacterial generalists and specialists in lakes and reservoirs along a latitudinal gradient [J]. | GLOBAL ECOLOGY AND BIOGEOGRAPHY , 2023 , 32 (11) : 2017-2032 . |
| MLA | Zuo, Jun et al. "Patterns of bacterial generalists and specialists in lakes and reservoirs along a latitudinal gradient" . | GLOBAL ECOLOGY AND BIOGEOGRAPHY 32 . 11 (2023) : 2017-2032 . |
| APA | Zuo, Jun , Liu, Lemian , Xiao, Peng , Xu, Zijie , Wilkinson, David M. , Grossart, Hans-Peter et al. Patterns of bacterial generalists and specialists in lakes and reservoirs along a latitudinal gradient . | GLOBAL ECOLOGY AND BIOGEOGRAPHY , 2023 , 32 (11) , 2017-2032 . |
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本发明公开了一种用于赤潮防控的皂苷基多孔泡沫混凝土，该皂苷基多孔泡沫混凝土是由凝胶材料和泡沫材料组成，其中，所述凝胶材料由水泥与水混合制得，所述泡沫材料是将茶皂素水溶液经发泡制得。本发明所得皂苷基多孔泡沫混凝土具有密度低、孔隙率和吸水率高、孔径和比表面积大等特点，可通过直接吸附赤潮藻或固定化吸附溶藻菌来降低赤潮藻生物量，起到赤潮防控的效果。
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| GB/T 7714 | 陈剑锋 , 吴桐 , 郭毅松 et al. 一种用于赤潮防控的皂苷基多孔泡沫混凝土 : CN202211140853.6[P]. | 2022-09-20 00:00:00 . |
| MLA | 陈剑锋 et al. "一种用于赤潮防控的皂苷基多孔泡沫混凝土" : CN202211140853.6. | 2022-09-20 00:00:00 . |
| APA | 陈剑锋 , 吴桐 , 郭毅松 , 石新国 , 刘乐冕 . 一种用于赤潮防控的皂苷基多孔泡沫混凝土 : CN202211140853.6. | 2022-09-20 00:00:00 . |
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本发明公开了一种用于红树植物种植的植生混凝土，由多孔混凝土外框和植被混凝土内核两部分组成。使用时，先将红树植物种苗种植在植被混凝土内核中，再将内核填充到多孔混凝土外框中，以实现红树植物的种植。本发明提供的植生混凝土利用多孔混凝土外框的强度和密度起到减缓海浪潮汐冲刷的作用，且外框较高的孔隙率可有效提高红树植物的透水透气性，而植被混凝土内核具有适宜的强度、密度和孔隙率，不但可防止种植基质流失、助力红树植物固着抗倒伏，而且可大大提高红树植物的透水透气性、存活率和保有率。
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| GB/T 7714 | 石新国 , 陈冬杰 , 陈剑锋 et al. 一种用于红树植物种植的植生混凝土 : CN202211140854.0[P]. | 2022-09-20 00:00:00 . |
| MLA | 石新国 et al. "一种用于红树植物种植的植生混凝土" : CN202211140854.0. | 2022-09-20 00:00:00 . |
| APA | 石新国 , 陈冬杰 , 陈剑锋 , 刘乐冕 , 郭毅松 . 一种用于红树植物种植的植生混凝土 : CN202211140854.0. | 2022-09-20 00:00:00 . |
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目的探明银合欢幼苗在植物工厂中水培的最优生长条件，为生产优质的银合欢苗奠定基础。方法分析不同光照时长（12、16、20、24 h·d−1）、光照强度（100、200、300、400 μmol·m−2·s−1）、营养液盐度（7‰、15‰、20‰、25‰）、氮浓度（7.5、15、30、60 mmol·L−1）和磷浓度（0.5、1、2、4 mmol·L−1）下银合欢幼苗的形态和生理指标，优化银合欢幼苗的生长条件。结果单因素试验结果表明，随着光照时长、光照强度、氮浓度或磷浓度的增加，银合欢幼苗的形态指标呈现先增后降的趋势。幼苗定植35 d后，在光照时长20 h·d−1下幼苗鲜重（5.61±0.11） g·株−1，比12、24 h·d−1分别增加60.0%和14.6%；光强200 μmol·m−2·s−1下鲜重（6.55±0.10） g·株−1，比100、400 μmol·m−2·s−1分别增加21.5%和62.1%；氮浓度15 mmol·L−1下鲜重（4.32±0.10） g·株−1，比7.5、60 mmol·L−1分别增加6.1%和108.6%；磷浓度1 mmol·L−1下鲜重（5.65±0.21） g·株−1，比0.5、4 mmol·L−1分别增加40.9%和64.7%。银合欢幼苗的叶绿素含量、叶绿素荧光参数和抗氧化酶活性也表现出相似的规律。相比之下，随着营养液盐度的增加，银合欢幼苗的形态指标、叶绿素含量、叶绿素荧光参数和抗氧化酶活性均呈现下降趋势。在盐度7‰时鲜重、干重、株高、根长和总叶绿素含量均达到最大值，分别为（8.95±0.05） g·株−1、（2.16±0.16） g·株−1、（31.17±1.67） cm、（60.67±0.93） cm、（1.72±0.06） mg·g−1。7‰盐度下幼苗的叶绿素荧光参数和抗氧化酶活性指标也较好。结论银合欢幼苗在光照时长20 h·d−1、光强200 μmol·m−2·s−1、盐度7‰、氮浓度15 mmol·L−1、磷浓度1 mmol·L−1 的条件下，其形态指标、叶绿素含量、抗氧化酶活性及叶绿素荧光参数等指标较优，最适合银合欢幼苗生长。
Keyword ：
光照 光照 植物工厂 植物工厂 盐度 盐度 营养液氮磷 营养液氮磷 银合欢 银合欢
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| GB/T 7714 | 庄朋静 , 李娜 , 刘乐冕 et al. 植物工厂中银合欢幼苗生长条件的优化 [J]. | 福建农业学报 , 2023 , 38 (2) : 183-192 . |
| MLA | 庄朋静 et al. "植物工厂中银合欢幼苗生长条件的优化" . | 福建农业学报 38 . 2 (2023) : 183-192 . |
| APA | 庄朋静 , 李娜 , 刘乐冕 , 郭毅松 , 陈剑锋 . 植物工厂中银合欢幼苗生长条件的优化 . | 福建农业学报 , 2023 , 38 (2) , 183-192 . |
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