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学者姓名：陈晓晨
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Abstract ：
Whether coexisting microplastics (MPs) affect the ecological and health risks of cadmium (Cd) in soils is a cutting-edge scientific issue. In this study, four typical Chinese soils were prepared as artificially Cdcontaminated soils with/without aged polystyrene (PS). TCLP and in vitro PBET model were used to determine the leachability (ecological risk) and oral bioaccessibility (human health risk) of soil Cd. The mechanisms by which MPs influence soil Cd were discussed from direct and indirect perspectives. Results showed that there was no significant difference in the leachability of soil Cd with/without aged PS. Additionally, aged PS led to a significant decrease in the bioaccessibility of soil Cd in gastric phase, but not in small intestinal phase. The increase in surface roughness and the new characteristic peaks (e.g., Si -O -Si) of aged PS directly accounted for the change in Cd bioaccessibility. The change in organic matter content indirectly accounted for the exceptional increase in Cd bioaccessibility of black soil with aged PS in small intestinal phase. Furthermore, the changes in cation exchange capacity and Cd mobility factor caused by aged PS explained the change in Cd leachability. These results contribute to a deeper understanding about environmental and public health in complicated emerging scenarios.
Keyword ：
Bioaccessibility Bioaccessibility Cd Cd in vitro test in vitro test Leachability Leachability Microplastics Microplastics
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| GB/T 7714 | Chen, Xiao-Chen , Wang, Ao , Wang, Jun-Jie et al. Influences of coexisting aged polystyrene microplastics on the ecological and health risks of cadmium in soils: A leachability and oral bioaccessibility based study [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 469 . |
| MLA | Chen, Xiao-Chen et al. "Influences of coexisting aged polystyrene microplastics on the ecological and health risks of cadmium in soils: A leachability and oral bioaccessibility based study" . | JOURNAL OF HAZARDOUS MATERIALS 469 (2024) . |
| APA | Chen, Xiao-Chen , Wang, Ao , Wang, Jun-Jie , Zhang, Zeng-Di , Yu, Jian-Ying , Yan, Ying-Jie et al. Influences of coexisting aged polystyrene microplastics on the ecological and health risks of cadmium in soils: A leachability and oral bioaccessibility based study . | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 469 . |
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The production of isolated metallic nanoparticles with multifunctionalized properties, such as size and shape, is crucial for biomedical, photocatalytic, and energy storage or remediation applications. This study investigates the initial particle formations of gold nanoparticles (AuNPs) bioproduced in the cyanobacteria Anabaena sp. using high-resolution transmission electron microscopy images for digital image analysis. The developed method enabled the discovery of cerium nanoparticles (CeNPs), which were biosynthesized in the cyanobacteria Calothrix desertica. The particle size distributions for AuNPs and CeNPs were analyzed. After 10 h, the average equivalent circular diameter for AuNPs was 4.8 nm, while for CeNPs, it was approximately 5.2 nm after 25 h. The initial shape of AuNPs was sub-round to round, while the shape of CeNPs was more roundish due to their amorphous structure and formation restricted to heterocysts. The local PSDs indicate that the maturation of AuNPs begins in the middle of vegetative cells and near the cell membrane, compared to the other regions of the cell.
Keyword ：
Anabaena sp. Anabaena sp. biorecovery biorecovery biosynthesis biosynthesis Calothrix desertica Calothrix desertica digital image analysis digital image analysis TEM TEM
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| GB/T 7714 | Fritz, Melanie , Chen, Xiaochen , Yang, Guifang et al. Gold Nanoparticles Bioproduced in Cyanobacteria in the Initial Phase Opened an Avenue for the Discovery of Corresponding Cerium Nanoparticles [J]. | MICROORGANISMS , 2024 , 12 (2) . |
| MLA | Fritz, Melanie et al. "Gold Nanoparticles Bioproduced in Cyanobacteria in the Initial Phase Opened an Avenue for the Discovery of Corresponding Cerium Nanoparticles" . | MICROORGANISMS 12 . 2 (2024) . |
| APA | Fritz, Melanie , Chen, Xiaochen , Yang, Guifang , Lv, Yuancai , Liu, Minghua , Wehner, Stefan et al. Gold Nanoparticles Bioproduced in Cyanobacteria in the Initial Phase Opened an Avenue for the Discovery of Corresponding Cerium Nanoparticles . | MICROORGANISMS , 2024 , 12 (2) . |
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Arsenic (As)-contaminated soil poses great health risk to human mostly through inadvertent oral exposure. We investigated CaAl-layered double hydroxide (CaAl-LDH), a promising immobilising agent, for the remediation of As-contaminated Chinese soils. The effects on specific soil properties and As fractionation were analyzed, and changes in the health risk of soil As were accurately assessed by means of advanced in vivo mice model and in vitro PBET-SHIME model. Results showed that the application of CaAl-LDH significantly increased soil pH and concentration of Fe and Al oxides, and effectively converted active As fractions into the most stable residual fraction, guaranteeing long-term remediation stability. Based on in vivo test, As relative bioavailability was significantly reduced by 37.75%. Based on in vitro test, As bioaccessibility in small intestinal and colon phases was significantly reduced by 25.65% and 28.57%, respectively. Furthermore, As metabolism (reduction and methylation) by the gut microbiota inhabiting colon was clearly observed. After immobilisation with CaAl-LDH, the concentration of bioaccessible As(V) in the colon fluid was significantly reduced by 61.91%, and organic As (least toxic MMA(V) and DMA(V)) became the main species, which further reduced the health risk of soil As. In summary, CaAl-LDH proved to be a feasible option for immobilisation remediation of As -contaminated soils, and considerable progress was made in relevant health risk assessment.
Keyword ：
Arsenic Arsenic CaAl-layered double hydroxide CaAl-layered double hydroxide Health risk Health risk Immobilisation Immobilisation Soil remediation Soil remediation
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| GB/T 7714 | Chen, Xiao-Chen , Huang, Zhen-Jia , Wang, Ao et al. Immobilisation remediation of arsenic-contaminated soils with promising CaAl-layered double hydroxide and bioavailability, bioaccessibility, and speciation-based health risk assessment [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 469 . |
| MLA | Chen, Xiao-Chen et al. "Immobilisation remediation of arsenic-contaminated soils with promising CaAl-layered double hydroxide and bioavailability, bioaccessibility, and speciation-based health risk assessment" . | JOURNAL OF HAZARDOUS MATERIALS 469 (2024) . |
| APA | Chen, Xiao-Chen , Huang, Zhen-Jia , Wang, Ao , Yu, Jian-Ying , Zhang, Jian-Yu , Xiao, Zi-Jun et al. Immobilisation remediation of arsenic-contaminated soils with promising CaAl-layered double hydroxide and bioavailability, bioaccessibility, and speciation-based health risk assessment . | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 469 . |
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Keyword ：
advanced technologies/systems advanced technologies/systems conventional contaminants conventional contaminants emerging contaminants emerging contaminants environmental engineering environmental engineering microorganisms microorganisms municipal engineering municipal engineering
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| GB/T 7714 | Chen, Xiaochen , Kasuga, Ikuro , Liu, Xianhua et al. New insight into the roles of microorganisms in municipal and environmental engineering technologies/systems [J]. | FRONTIERS IN MICROBIOLOGY , 2024 , 14 . |
| MLA | Chen, Xiaochen et al. "New insight into the roles of microorganisms in municipal and environmental engineering technologies/systems" . | FRONTIERS IN MICROBIOLOGY 14 (2024) . |
| APA | Chen, Xiaochen , Kasuga, Ikuro , Liu, Xianhua , Li, Hongbo , Zeng, Ming , Cai, Xiaolin . New insight into the roles of microorganisms in municipal and environmental engineering technologies/systems . | FRONTIERS IN MICROBIOLOGY , 2024 , 14 . |
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针对低温时饮用水深度处理工艺生物活性炭（BAC）无法高效去除水中氨氮的问题，选用实际水厂运行中的BAC搭建滤柱连续培养反应器，通过室内控制实验，对比常温和低温下不同层BAC的氨氮去除效果及其硝化潜势，并运用分子生物学技术解析BAC上氨氧化古菌（AOA）和氨氧化细菌（AOB）的群落丰度和群落结构。结果表明，尽管低温时BAC各层去除氨氮的效率低于常温，但在一个反冲洗周期的后段仍能够达到常温时的氨氮去除效果，且其硝化潜势高于常温。低温下BAC上氨氧化微生物丰度高于常温，且AOA的数量变化最大（增加2个数量级）。针对功能基因amoA的T-RFLP分析发现，A-OTU2和A-OTU3以及B-OTU5分别为低温下主导的AOA和AOB。今后应更加关注低温下AOA及特定菌种在BAC上的定植及活性促进问题。
Keyword ：
氨氧化古菌 氨氧化古菌 氨氧化细菌 氨氧化细菌 氨氮 氨氮 温度 温度 生物活性炭 生物活性炭 群落结构 群落结构
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| GB/T 7714 | 牛佳 , 余爽 , 汤旸旸 et al. 温度影响BAC去除饮用水中氨氮的生物学机理 [J]. | 中国给水排水 , 2024 , 40 (15) : 44-49 . |
| MLA | 牛佳 et al. "温度影响BAC去除饮用水中氨氮的生物学机理" . | 中国给水排水 40 . 15 (2024) : 44-49 . |
| APA | 牛佳 , 余爽 , 汤旸旸 , 黄怡婷 , 龚珑聪 , 王悦 et al. 温度影响BAC去除饮用水中氨氮的生物学机理 . | 中国给水排水 , 2024 , 40 (15) , 44-49 . |
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Dissolved organic matter (DOM) is important in determining the drinking water treatment and the supplied water quality. However, a comprehensive DOM study for the whole water supply system is lacking and the potential effects of secondary water supply are largely unknown. This was studied using dissolved organic carbon (DOC), absorption spectroscopy, and fluorescence excitation -emission matrices -parallel factor analysis (EEMPARAFAC). Four fluorescent components were identified, including humic-like C1-C2, tryptophan-like C3, and tyrosine -like C4. In the drinking water treatment plants, the advanced treatment using ozone and biological activated carbon (O3-BAC) was more effective in removing DOC than the conventional process, with the removals of C1 and C3 improved by 17.7%-25.1% and 19.2%-27.0%. The absorption coefficient and C1-C4 correlated significantly with DOC in water treatments, suggesting that absorption and fluorescence could effectively track the changes in bulk DOM. DOM generally remained stable in each drinking water distribution system, suggesting the importance of the treated water quality in determining that of the corresponding network. The optical indices changed notably between distribution networks of different treatment plants, which enabled the identification of changing water sources. A comparison of DOM in the direct and secondary water supplies suggested limited impacts of secondary water supply, although the changes in organic carbon and absorption indices were detected in some locations. These results have implications for better understanding the changes of DOM in the whole water supply system to help ensure the supplied water quality.
Keyword ：
Dissolved organic matter Dissolved organic matter Drinking water treatment Drinking water treatment Fluorescence excitation -emission matrices Fluorescence excitation -emission matrices Parallel factor analysis Parallel factor analysis Water supply system Water supply system
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| GB/T 7714 | Lin, Jinjin , Yang, Liyang , Zhuang, Wan-E et al. Tracking the changes of dissolved organic matter throughout the city water supply system with optical indices [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2024 , 358 . |
| MLA | Lin, Jinjin et al. "Tracking the changes of dissolved organic matter throughout the city water supply system with optical indices" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 358 (2024) . |
| APA | Lin, Jinjin , Yang, Liyang , Zhuang, Wan-E , Wang, Yue , Chen, Xiaochen , Niu, Jia . Tracking the changes of dissolved organic matter throughout the city water supply system with optical indices . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2024 , 358 . |
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Aquatic products decrease environmental burdens and improve human health by supplying various essential nutrients, while their contamination with metal(loid)s could pose health risks to humans through food chain. In this study, the concentrations, trophic magnification and health risks of multiple metal(loid)s in various aquatic products were analyzed, including arsenic, cadmium, mercury, lead, cobalt, manganese, iron, copper, nickel and zinc. The results showed that the total concentrations of the metal(loid)s in marine foods from the same sea area were in the following order: seaweeds > shellfish > cephalopods > crustaceans > saltwater fish > freshwater fish. Arsenic speciation analysis indicated that high concentrations of arsenolipids were detected in lower trophic organisms. The maximum level of organoarsenic in seafood should be evaluated because of the potential toxicity of arsenolipids and arsenosugar metabolites. Weekly intake of metal(loid)s was far below the recommended provisional tolerable weekly intake even with excessive consumption of aquatic products. The hazard index was above 1 for excessive consumers, while our results also indicated that the health risks from aquatic products could be sharply decreased by reducing the ingestion of seaweeds and cephalopods. The study provided valuable guidance on the consumption of aquatic products by the general public.(GRAPHICS)
Keyword ：
Arsenic speciation Arsenic speciation Carcinogenic and non-carcinogenic effects Carcinogenic and non-carcinogenic effects Metal(loid)s Metal(loid)s Provisional tolerable weekly intake Provisional tolerable weekly intake
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| GB/T 7714 | Hu, Shi-Lin , Chen, Xiao-Chen , Xue, Ying-Xin et al. Metal(Loid)s in Aquatic Products and Their Potential Health Risk [J]. | EXPOSURE AND HEALTH , 2023 , 16 (1) : 57-70 . |
| MLA | Hu, Shi-Lin et al. "Metal(Loid)s in Aquatic Products and Their Potential Health Risk" . | EXPOSURE AND HEALTH 16 . 1 (2023) : 57-70 . |
| APA | Hu, Shi-Lin , Chen, Xiao-Chen , Xue, Ying-Xin , Zhi, Li-Ying , Yang, Yu-Han , Zhu, Yong-Guan et al. Metal(Loid)s in Aquatic Products and Their Potential Health Risk . | EXPOSURE AND HEALTH , 2023 , 16 (1) , 57-70 . |
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Herein, the particle size distributions (PSDs) and shape analysis of in vivo bioproduced particles from aqueous Au3+ and Eu3+ solutions by the cyanobacterium Anabaena sp. are examined in detail at the nanoscale. Generally, biosynthesis is affected by numerous parameters. Therefore, it is challenging to find the key set points for generating tailored nanoparticles (NPs). PSDs and shape analysis of the Au and Eu-NPs were performed with ImageJ using high-resolution transmission electron microscopy (HR-TEM) images. As the HR-TEM image analysis reflects only a fraction of the detected NPs within the cells, additional PSDs of the complete cell were performed to determine the NP count and to evaluate the different accuracies. Furthermore, local PSDs were carried out at five randomly selected locations within a single cell to identify local hotspots or agglomerations. The PSDs show that particle size depends mainly on contact time, while the particle shape is hardly affected. The particles formed are distributed quite evenly within the cells. HR-PSDs for Au-NPs show an average equivalent circular diameter (ECD) of 8.4 nm (24 h) and 7.2 nm (51 h). In contrast, Eu-NPs preferably exhibit an average ECD of 10.6 nm (10 h) and 12.3 nm (244 h). Au-NPs are classified predominantly as "very round" with an average reciprocal aspect ratio (RAR) of similar to 0.9 and a Feret major axis ratio (FMR) of similar to 1.17. Eu-NPs mainly belong to the "rounded" class with a smaller RAR of similar to 0.6 and a FMR of similar to 1.3. These results show that an increase in contact time is not accompanied by an average particle growth for Au-NPs, but by a doubling of the particle number. Anabaena sp. is capable of biosorbing and bioreducing dissolved Au3+ and Eu3+ ions from aqueous solutions, generating nano-sized Au and Eu particles, respectively. Therefore, it is a low-cost, non-toxic and effective candidate for a rapid recovery of these sought-after metals via the bioproduction of NPs with defined sizes and shapes, providing a high potential for scale-up.
Keyword ：
cyanobacteria cyanobacteria digital image processing digital image processing growth monitoring growth monitoring nanoparticle size distribution nanoparticle size distribution shape classification shape classification
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| GB/T 7714 | Fritz, Melanie , Koersten, Susanne , Chen, Xiaochen et al. Time-Dependent Size and Shape Evolution of Gold and Europium Nanoparticles from a Bioproducing Microorganism, a Cyanobacterium: A Digitally Supported High-Resolution Image Analysis [J]. | NANOMATERIALS , 2023 , 13 (1) . |
| MLA | Fritz, Melanie et al. "Time-Dependent Size and Shape Evolution of Gold and Europium Nanoparticles from a Bioproducing Microorganism, a Cyanobacterium: A Digitally Supported High-Resolution Image Analysis" . | NANOMATERIALS 13 . 1 (2023) . |
| APA | Fritz, Melanie , Koersten, Susanne , Chen, Xiaochen , Yang, Guifang , Lv, Yuancai , Liu, Minghua et al. Time-Dependent Size and Shape Evolution of Gold and Europium Nanoparticles from a Bioproducing Microorganism, a Cyanobacterium: A Digitally Supported High-Resolution Image Analysis . | NANOMATERIALS , 2023 , 13 (1) . |
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Arsenic (As) mobilisation assists in remediating As-contaminated soils but might increase ecological and health risks. In this study, risks of applying two mobilising agents were assessed, i.e. an emerging reducing-chelating composite agent [dithionite (Na2S2O4)-EDTA] and a classical low-molecular-weight organic acid (LMWOA) [citric acid (C6H8O7)]. Results showed that both agents induced sharp increase in leachability-based ecological risk of As. Interestingly, the two agents had opposite performances regarding health risks. Na2S2O4-EDTA significantly increased As relative bioavailability (RBA) to 1.83 times that in controls based on in vivo mouse model, and As bioaccessibility to 1.96, 1.65 and 1.20 times in gastric, small intestinal and colon phases based on in vitro PBET-SHIME model. Besides, it caused significant increase of highly toxic As(III) in colon fluid. In contrast, C6H8O7 significantly reduced RBA and bioaccessibility of soil As in colon by 44.44% and 14.65%, respectively. Importantly, C6H8O7 restrained bioaccessible As(V) reduction and promoted bioaccessible As(III) methylation, further reducing health risk. The phenomena could mainly be attributed to excessive metal com-ponents release from soil by C6H8O7 and gut microbiota metabolism of C6H8O7. In summary, C6H8O7 and similar LMWOAs are recommended. The study contributes to mobilising agent selection and development and provides a reference for managing remediation sites.
Keyword ：
Ecological risk Ecological risk Gut microbiota Gut microbiota Health risk Health risk Mobilisation Mobilisation Soil arsenic Soil arsenic
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| GB/T 7714 | Chen, Xiao-Chen , Yao, Cong-Cong , Wang, Ao et al. Risks of applying mobilising agents for remediation of arsenic-contaminated soils: Effects of dithionite-EDTA and citric acid on arsenic fractionation, leachability, oral bioavailability/bioaccessibility and speciation [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 444 . |
| MLA | Chen, Xiao-Chen et al. "Risks of applying mobilising agents for remediation of arsenic-contaminated soils: Effects of dithionite-EDTA and citric acid on arsenic fractionation, leachability, oral bioavailability/bioaccessibility and speciation" . | JOURNAL OF HAZARDOUS MATERIALS 444 (2023) . |
| APA | Chen, Xiao-Chen , Yao, Cong-Cong , Wang, Ao , Zhang, Zeng-Di , Chen, Long -Zhao , Zhang, Jian-Yu et al. Risks of applying mobilising agents for remediation of arsenic-contaminated soils: Effects of dithionite-EDTA and citric acid on arsenic fractionation, leachability, oral bioavailability/bioaccessibility and speciation . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 444 . |
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Biofilms on the inner surface of a drinking water distribution system (DWDS) affect water quality and stability. Understanding the niche differentiation of biofilm microbial communities is necessary for the efficient control of DWDS biofilms. However, biofilm studies are difficult to conduct in the actual DWDS because of inaccessibility to the pipes buried underground. Taking the opportunity of infrastructure construction and relevant pipeline replacement in China, biofilms in a DWDS (a water main and its branch pipes) were collected in situ, followed by analysis on the abundances and community structures of bacterial and archaeal using quantitative PCR and high-throughput sequencing, respectively. Results showed that archaea were detected only in the biofilms of the water main, with a range of 9.4x10(3)similar to 1.1x10(5) copies/cm(2). By contrast, bacteria were detected in the biofilms of branch pipes and the distal part of the water main, with a range of 8.8x10(3)similar to 9.6x10(6) copies/cm(2). Among the biofilm samples, the archaeal community in the central part of the water main showed the highest richness and diversity. Nitrosopumilus was found to be predominant (86.22%) in the biofilms of the proximal part of the water main. However, Methanobrevibacter (87.15%) predominated in the distal part of the water main. The bacterial community of the water main and branch pipes was primarily composed of Firmicutes and Proteobacteria at the phylum level, respectively. Regardless of archaea or bacteria, only few operational taxonomic units (OTUs) (<0.5% of total OTUs) were shared by all the biofilms, indicating the niche differentiation of biofilm microorganisms. Moreover, the high Mn content in the biofilms of the distal sampling location (D3) in the water main was linked to the predominance of Bacillus. Functional gene prediction revealed that the proportion of infectious disease-related genes was 0.44-0.67% in the tested biofilms. Furthermore, functional genes related to the resistance of the bacterial community to disinfections and antibiotics were detected in all the samples, that is, glutathione metabolism-relating genes (0.14-0.65%) and beta-lactam resistance gene (0.01-0.05%). The results of this study indicate the ubiquity of archaea and bacteria in the biofilms of water main and branch pipes, respectively, and pipe diameters could be a major influencing factor on bacterial community structure. In the water main, the key finding was the predominant existence of archaea, particularly Nitrosopumilus and methanogen. Hence, their routine monitoring and probable influences on water quality in pipelines with large diameter should be given more attention. Besides, since Mn-related Bacillus and suspected pathogenic Enterococcus were detected in the biofilm, supplementation of disinfectant may be a feasible strategy for inhibiting their growth and ensuring water quality. In addition, the monitoring on their abundance variation could help to determine the frequency and methods of pipeline maintenance.
Keyword ：
Archaea Archaea Bacteria Bacteria Biofilm Biofilm Community diversity Community diversity Drinking water distribution system Drinking water distribution system Water main Water main
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| GB/T 7714 | Niu, Jia , Chen, Daogan , Shang, Chenghao et al. Niche Differentiation of Biofilm Microorganisms in a Full-scale Municipal Drinking Water Distribution System in China and Their Implication for Biofilm Control [J]. | MICROBIAL ECOLOGY , 2023 , 86 (4) : 2770-2780 . |
| MLA | Niu, Jia et al. "Niche Differentiation of Biofilm Microorganisms in a Full-scale Municipal Drinking Water Distribution System in China and Their Implication for Biofilm Control" . | MICROBIAL ECOLOGY 86 . 4 (2023) : 2770-2780 . |
| APA | Niu, Jia , Chen, Daogan , Shang, Chenghao , Xiao, Liang , Wang, Yue , Zeng, Wuqiang et al. Niche Differentiation of Biofilm Microorganisms in a Full-scale Municipal Drinking Water Distribution System in China and Their Implication for Biofilm Control . | MICROBIAL ECOLOGY , 2023 , 86 (4) , 2770-2780 . |
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