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学者姓名：吴元子
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Abstract ：
In view of the biological significance and micro-heterogeneity of protein glycosylation for human health, specific enrichment of N-glycosylated proteins/peptides from complex biological samples is a prerequisite for the discovery of disease biomarkers and clinical diagnosis. In this work, we propose a "grafting-from" N-glycoprotein enriching method based on the in-situ growth of thermoresponsive polymer brushes from the N-glycosylated site of proteins. The initiator was first attached to the pre-oxidized glycan moieties by hydrazide chemistry, from which the thermoresponsive polymers can be grown to form giant protein-polymer conjugates (PPC). The thermosensitive PPC can be precipitated and separated by raising the temperature to above its lower critical solubility temperature (LCST). Mass spectrometry verified 210 N-glycopeptides corresponding to 136 N-glycoproteins in the rabbit serum. These results demonstrate the capability of the tandem thermoprecipitation strategy to enrich and separate N-glycoprotein/glycopeptide. Due to its simplicity and efficiency specifically, this method holds the potential for identifying biomarkers from biological samples in N-glycoproteome analysis.
Keyword ：
Glycan-selective Glycan-selective glycopeptide glycopeptide N-Glycoprotein N-Glycoprotein Thermoprecipitation Thermoprecipitation
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| GB/T 7714 | Shu, Jingjing , Xiong, Wenli , Zhang, Ran et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides [J]. | TALANTA , 2023 , 253 . |
| MLA | Shu, Jingjing et al. "Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides" . | TALANTA 253 (2023) . |
| APA | Shu, Jingjing , Xiong, Wenli , Zhang, Ran , Ma, Shanyun , Zhou, Kaiqiang , Wang, Xuwei et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides . | TALANTA , 2023 , 253 . |
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Non-alcoholic fatty liver disease (NAFLD) has become a globally rising issue that can cause liver-related morbidity and mortality. Recent studies have revealed that a high-fat diet (HFD) highly contributes to the prevalence and progression of NAFLD via impacting gut microbiota and lipid metabolism signal pathways. Resveratrol (RSV), a natural bioactive compound, has exhibited potential for preventing and alleviating NAFLD. However, due to the poor bioavailability of RSV, its strengths and underlying mechanisms for NAFLD therapeutic potential are poorly understood. To address this, we utilized Hydroxypropyl-beta-Cyclodextrin (HBC) to encapsulate RSV to enhance its water solubility and conducted prevention and intervention experiments in HFDfed mice. The results showed that the HBC has significantly enhanced the water solubility of RSV by 250-fold and the HBC-RSV better prevented and alleviated the liver steatosis, obesity and abnormal lipid metabolism induced by HFD than RSV alone. Meanwhile, combining HFD and HBC-RSV or RSV prevented HFD mice progressing to NAFLD. Besides, further investigation indicated that RSV could resist liver injury and obesity by modulating gut microbiota, raising the levels of short-chain fatty acids (SCFAs) and activating AMP-activated protein kinase (AMPK) signaling pathway. The activated pathway down-regulated sterol receptor element binding protein 1c (SREBP1c) and acetyl-coenzyme A carboxylase (ACC) to decrease lipid synthesis and up-regulated peroxisome proliferators-activated receptor & alpha; (PPAR & alpha;) to promote the fatty acid oxidation, thus preventing NAFLD. Our findings suggested that water solubility-enhanced RSV beneficially modulated gut microbiota, altered gut microbiota-derived SCFAs, and activated lipid metabolism regulatory pathways, providing potential for NAFLD prevention and alleviation.
Keyword ：
AMPK AMPK Gut microbiota Gut microbiota Hydroxypropyl-beta-Cyclodextrin Hydroxypropyl-beta-Cyclodextrin NAFLD NAFLD resveratrol resveratrol SCFAs SCFAs
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| GB/T 7714 | Ke, Wenya , Huang, Juan , Zhong, Yi et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway [J]. | FOOD BIOSCIENCE , 2023 , 54 . |
| MLA | Ke, Wenya et al. "Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway" . | FOOD BIOSCIENCE 54 (2023) . |
| APA | Ke, Wenya , Huang, Juan , Zhong, Yi , Shi, Yuhong , Yan, Fen , Huang, Da et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway . | FOOD BIOSCIENCE , 2023 , 54 . |
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Bacterial infection of wounds is one of great concern to patients, and rapid and correct detection of bacterial infection is crucial to ensure accurate diagnosis and early intervention. Based on the principle that glucose can only be consumed by live bacteria, a fluorescent biosensor was constructed to detect four kinds of common infectious bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in skin wounds, taking advantage of magnetic bead-aptamer for recognizing, sorting and enrichment, platinum nanoparticles for signal amplification. The linear detection range of AB, EC, PA and SA were 27- 8 x 106 CFU/mL, 10- 2.5 x 107 CFU/mL, 34- 2.5 x 107 CFU/mL and 10- 1.0 x 107 CFU/mL, respectively, and the limits of detection were 27 CFU/mL, 10 CFU/mL, 34 CFU/mL and 2 CFU/mL. Furthermore, all target bacteria in samples containing 8 x 108 CFU/mL of other interfering bacteria have been successfully identified and quantified. The proposed method was also successfully applied to the detection of bacterial infection in skin wounds of mouse and human, including the detection of separate bacterial infection as well as a coinfection. The recovery of this method was in the range of 90.161- 109.961%. Thus, this proposed method can be a promising candidate for rapid and convenient evaluation of infectious bacteria in point-of-care settings.
Keyword ：
Aptamer Aptamer Bacterial infection Bacterial infection Fluorescent biosensor Fluorescent biosensor Platinum nanoparticles Platinum nanoparticles Wound Wound
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| GB/T 7714 | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
| MLA | Gao, Lanmei et al. "Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds" . | SENSORS AND ACTUATORS B-CHEMICAL 393 (2023) . |
| APA | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong , Zhong, Yi , Jiang, Linhai , Wu, Yuanzi et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
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Inflammation is a critical immune response to various stimuli but can lead to diseases when uncontrolled. Commonly used anti-inflammatory drugs usually associated with side effects, natural products such as curcumin (CUR) have emerged as a promising alternative but suffer from low bioavailability. This study introduces a novel nanocarrier, amphiphilic hyperbranched polyprodrug (P(ACPP-co-CURMA)-b-POEGMA, PACE), designed to enhance the anti-inflammatory activity of CUR. PACE micelles, formed from a hydrophobic hyperbranched polyprodrug core and hydrophilic POEGMA corona, demonstrated remarkable stability, even upon dilution, and efficiently released CUR in response to the reductive intracellular environment. These micelles were biocompatible and effectively internalized by macrophages. In both in vitro and in vivo studies, PACE micelles demonstrated significant anti-inflammatory activity, reducing ROS levels, down-regulating pro-inflammatory cytokines, and alleviating inflammation. This work highlights the potential of PACE micelles as a promising platform for targeted and responsive anti-inflammatory therapy, with implications for treating inflammatory disorders.
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| GB/T 7714 | Huang, Da , Tang, Qiang , Zou, Minglang et al. Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo [J]. | POLYMER CHEMISTRY , 2023 , 14 (47) : 5208-5217 . |
| MLA | Huang, Da et al. "Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo" . | POLYMER CHEMISTRY 14 . 47 (2023) : 5208-5217 . |
| APA | Huang, Da , Tang, Qiang , Zou, Minglang , Wang, Yongming , Luo, Fang , Mu, Jinyi et al. Anti-inflammatory unimolecular micelles of redox-responsive hyperbranched polycurcumin amphiphiles with enhanced anti-inflammatory efficacy in vitro and in vivo . | POLYMER CHEMISTRY , 2023 , 14 (47) , 5208-5217 . |
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An abnormal microenvironment underlies poor healing in chronic diabetic chronic wounds. However, effectively modulating the microenvironment of the diabetic wound remains a great challenge due to sustained oxidative stress and chronic inflammation. Here, we present a unimolecular enzyme-polymer conjugate that demonstrates excellent multienzymatic cascade activities. The cascaded enzyme conjugates (CECs) were synthesized by grafting poly(N-acryloyl-lysine) (pLAAm) from the glycan moieties of glucose oxidase (GOx) via glycan-initiated polymerization. The resulting CECs exhibited multiple enzymatic properties of GOx, superoxide dismutase mimic, and catalase mimic activities simultaneously. The CECs facilitated the depletion of high blood glucose, ROS scavenging, bacteria-killing, anti-inflammatory effects, and sustained oxygen generation, which restored the microenvironment in diabetic wounds. In vivo results from a diabetic mouse model confirmed the capacity and efficiency of the cascade reaction for diabetic wound healing. Our findings demonstrate that the three-in-one enzyme-polymer conjugates alone can modulate the diabetic microenvironment for wound healing.
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| GB/T 7714 | Wu, Yuanzi , Lyu, Yanwei , Li, Ling et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing [J]. | BIOMACROMOLECULES , 2023 , 25 (1) : 43-54 . |
| MLA | Wu, Yuanzi et al. "Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing" . | BIOMACROMOLECULES 25 . 1 (2023) : 43-54 . |
| APA | Wu, Yuanzi , Lyu, Yanwei , Li, Ling , Zhou, Kaiqiang , Cai, Jingwen , Wang, Xuwei et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing . | BIOMACROMOLECULES , 2023 , 25 (1) , 43-54 . |
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本发明提供一种无泄漏封包酵母水凝胶及其制备方法。通过构建携带人源基因的酵母工程菌，所得工程酵母菌表面糖链进行原位氧化，引发，高分子的特异性交联制备获得无泄漏封包酵母水凝胶。该水凝胶可防止酵母菌的泄露，减小直接接触人体时可能产生的免疫反应并加速伤口愈合。本发明的优点：生物相容性佳，反应温和，应用前景广泛。
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| GB/T 7714 | 吴元子 , 蔡静雯 , 舒京晶 et al. 一种无泄漏封包酵母水凝胶及其制备方法 : CN202210103555.3[P]. | 2022-01-28 . |
| MLA | 吴元子 et al. "一种无泄漏封包酵母水凝胶及其制备方法" : CN202210103555.3. | 2022-01-28 . |
| APA | 吴元子 , 蔡静雯 , 舒京晶 , 李玲 , 周凯强 , 王旭伟 et al. 一种无泄漏封包酵母水凝胶及其制备方法 : CN202210103555.3. | 2022-01-28 . |
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Scope Depression is the leading cause of disability around the world; however, most antidepressants have drug tolerance and serious side effects. In this study, it is explored whether partially hydrolyzed guar gum (PHGG) is a safe food that exhibits protection in a mouse model of depression. Methods and Results PHGG is orally administered to mice with depression induced by chronic unpredictable mild stress (CUMS) in two animal experiments (prevention trial and intervention trial) to characterize the potentially protective effect of PHGG. The results in the prevention trial show that PHGG significantly inhibits the loss of body weight, and prevents CUMS-induced depressive-like behavior in mice. The beneficial effects may be associated with PHGG modulating the gut microbiota structure and then increasing the levels of short-chain fatty acids in mice feces and the levels of 5-hydroxytryptamine and dopamine in serum, striatum, and hippocampus. Besides, PHGG in the intervention trial is less effective than that in the prevention trial, but it may have a synergistic effect on improving depression with fluoxetine. Conclusions This study suggests that moderate daily intake of PHGG can contribute to relieving depressive-like behavior.
Keyword ：
depression depression gut microbiota gut microbiota neurotransmitter neurotransmitter partially hydrolyzed guar gum partially hydrolyzed guar gum short-chain fatty acids short-chain fatty acids
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| GB/T 7714 | Chen, Yanqiu , Wan, Mei , Zhong, Yi et al. Partially Hydrolyzed Guar Gum Modulates Gut Microbiota, Regulates the Levels of Neurotransmitters, and Prevents CUMS-Induced Depressive-Like Behavior in Mice [J]. | MOLECULAR NUTRITION & FOOD RESEARCH , 2021 , 65 (16) . |
| MLA | Chen, Yanqiu et al. "Partially Hydrolyzed Guar Gum Modulates Gut Microbiota, Regulates the Levels of Neurotransmitters, and Prevents CUMS-Induced Depressive-Like Behavior in Mice" . | MOLECULAR NUTRITION & FOOD RESEARCH 65 . 16 (2021) . |
| APA | Chen, Yanqiu , Wan, Mei , Zhong, Yi , Gao, Tingfang , Zhang, Yuehan , Yan, Fen et al. Partially Hydrolyzed Guar Gum Modulates Gut Microbiota, Regulates the Levels of Neurotransmitters, and Prevents CUMS-Induced Depressive-Like Behavior in Mice . | MOLECULAR NUTRITION & FOOD RESEARCH , 2021 , 65 (16) . |
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Antipyretic acetaminophen (APAP) is a commonly used drug that generally associates with liver injury. (-)-Epigallocatechin-3-gallate (EGCG), an active polyphenol extracted from green tea, is extensively reported to have the potential to impact a variety of human diseases. However, few studies were reported regarding the protective effect of EGCG on APAP-induced liver injury and the mechanism is still unclear. In this study, in-vitro and in-vivo experiments were carried out to verify the hepatoprotective effect of EGCG against APAP-induced liver injury and explore the potential mechanism. Results indicated that EGCG effectively relieved the liver injury caused by APAP, as well as APAP-induced mitochondrial dysfunction. The protective role of EGCG was not only attributed to its antioxidant capacity; but also might be related to the protective effect on hepatic mitochondrial impairment; based on that, EGCG could improve the membrane potential and activities of the respiratory chain complexes in liver mitochondria. Our study casts a new light on the mechanism of EGCG's hepatoprotective effect and suggests that EGCG has considerable potential in developing tonics for relieving APAP-induced liver injury.
Keyword ：
APAP APAP EGCG EGCG Liver Injury Liver Injury Mitochondria Mitochondria ROS ROS
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| GB/T 7714 | Lin, Yongxu , Huang, Juan , Gao, Tingfang et al. Preliminary Study on Hepatoprotective Effect and Mechanism of (-)-Epigallocatechin-3-gallate against Acetaminophen-induced Liver Injury in Rats [J]. | IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH , 2021 , 20 (3) : 46-56 . |
| MLA | Lin, Yongxu et al. "Preliminary Study on Hepatoprotective Effect and Mechanism of (-)-Epigallocatechin-3-gallate against Acetaminophen-induced Liver Injury in Rats" . | IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH 20 . 3 (2021) : 46-56 . |
| APA | Lin, Yongxu , Huang, Juan , Gao, Tingfang , Wu, Yuanzi , Huang, Da , Yan, Fen et al. Preliminary Study on Hepatoprotective Effect and Mechanism of (-)-Epigallocatechin-3-gallate against Acetaminophen-induced Liver Injury in Rats . | IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH , 2021 , 20 (3) , 46-56 . |
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Formation of protein-polymer conjugates (PPCs) is critical for many studies in chemical biology, biomedicine, and enzymatic catalysis. Polymers with coordinated physicochemical properties confer synergistic functions to PPCs that overcome the inherent limitation of proteins. However, application of PPCs has been synthetically restricted by the limited modification sites and polymer grafting method. Here, we present a versatile strategy for site-selective PPC synthesis. The initiator was specifically tethered to the preoxidized glycan moieties through oxime chemistry. Polymer brushes were grown in situ from the glycan by atom-transfer radical polymerization to generate well-controlled PPCs. Notably, the modification is site-specific, multivalent, and alterable depending on protein glycosylation. Additionally, we demonstrated that the cytocompatible method enabled the growth of polymer chains from the surface of living yeast cells. These results verified a facile technology for surface modification of biomacromolecules by desired polymers for various biomedical applications.
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| GB/T 7714 | Bi, Xueran , Xiong, Wenli , He, Jian'an et al. Site-Selective and Biocompatible Growth of Polymers from Glycan Moieties of Glycoproteins and Living Cells [J]. | BIOMACROMOLECULES , 2021 , 22 (10) : 4237-4243 . |
| MLA | Bi, Xueran et al. "Site-Selective and Biocompatible Growth of Polymers from Glycan Moieties of Glycoproteins and Living Cells" . | BIOMACROMOLECULES 22 . 10 (2021) : 4237-4243 . |
| APA | Bi, Xueran , Xiong, Wenli , He, Jian'an , Ma, Shanyun , Zhang, Jiangsheng , Fang, Yuan et al. Site-Selective and Biocompatible Growth of Polymers from Glycan Moieties of Glycoproteins and Living Cells . | BIOMACROMOLECULES , 2021 , 22 (10) , 4237-4243 . |
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Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.
Keyword ：
Acrylamide Acrylamide curcumin curcumin developmental toxicity developmental toxicity (-)- epigallocatechin gallate (-)- epigallocatechin gallate genotoxicity genotoxicity human lymphocytes human lymphocytes zebrafish embryos zebrafish embryos
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| GB/T 7714 | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity [J]. | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) : 199-218 . |
| MLA | Wang, Shanshan et al. "Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity" . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY 103 . 2 (2021) : 199-218 . |
| APA | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu , Liu, Jilai , He, Xiaozhen , Huang, Da et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) , 199-218 . |
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