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学者姓名：李婧影
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Abstract ：
RNA interference (RNAi) has been widely used in agriculture. However, it is well accepted that common methods of plant RNAi are species-dependent and lack systematic efficiency. This study designed a thiolated siRNA nanoparticle, guanidinium (Gu+)-containing disulfide assembled siRNA (Gu+-siRNA), demonstrating remarkable species independence and efficient systemic gene silencing across different plant species. Our results indicate that this approach effectively utilizes the plant vascular system to deliver siRNA, enabling long-distance gene silencing across both monocot and dicot plants, such as rice and Arabidopsis. By applying this method, we successfully targeted and silenced key genes like STM, WER, MYB23, GD1, EIL1, and EIL2, which regulate plant development and enhance salt tolerance. This delivery system significantly expands the application of RNAi technology across different plants, serving as a valuable tool for advancing agricultural biotechnology, enhancing crop resistance, and improving agricultural productivity, while aligning with global goals for sustainable food production and crop improvement.
Keyword ：
long-distance movement long-distance movement nanoparticle nanoparticle RNA interference RNA interference systematically deliver systematically deliver thiolated thiolated
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| GB/T 7714 | Lin, Shujin , Zhang, Qian , Bai, Shiyan et al. Beyond species and spatial boundaries: Enabling long-distance gene silencing in plants via guanidinium-siRNA nanoparticles [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) : 1165-1177 . |
| MLA | Lin, Shujin et al. "Beyond species and spatial boundaries: Enabling long-distance gene silencing in plants via guanidinium-siRNA nanoparticles" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 4 (2025) : 1165-1177 . |
| APA | Lin, Shujin , Zhang, Qian , Bai, Shiyan , Yang, Liwen , Qin, Guannan , Wang, Liyuan et al. Beyond species and spatial boundaries: Enabling long-distance gene silencing in plants via guanidinium-siRNA nanoparticles . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (4) , 1165-1177 . |
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The emergence of immunotherapy as a revolutionary therapeutic modality has fostered confidence and underscored its potent efficacy in tumor therapy. However, enhancing the therapeutic efficacy of immunotherapy by precise and judicious administration poses a significant challenge. In this context, we have developed a disulfide-bearing transdermal nanovaccine by integrating a thiol-reactive agent lipoic acid (LA) into a metal-coordinated cyclic dinucleotide nanoassembly, designated as LA-Mn-cGAMP (LMC) nanovaccines. Upon topical application to the skin with melanoma, the dithiolane moiety of LA enables thiol-disulfide dynamic exchange in the skin, hence facilitating penetration into both the skin and subcutaneous tumor tissues via the thiol-mediated uptake (TMU) mechanism. Our findings demonstrate that transdermal administration of LMC significantly enhances STING activation, mitigates the immunosuppressive tumor microenvironment (TME), and retards melanoma progression. Moreover, the remodeled TME amplifies the efficacy of immune checkpoint inhibitors. This advancement offers an administration strategy for existing STING agonist therapy, potentially improving the biosafety of immunotherapy. © 2025 American Chemical Society.
Keyword ：
Dermatitis Dermatitis Dermatology Dermatology Lanthanum compounds Lanthanum compounds Manganese alloys Manganese alloys Oncology Oncology
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| GB/T 7714 | Zhang, Junjie , Yang, Hui , Li, Liang et al. Noninvasive Transdermal Delivery of STING Agonists Reshapes the Immune Microenvironment of Melanoma and Potentiates Checkpoint Blockade Therapy Efficacy [J]. | ACS Applied Bio Materials , 2025 , 8 (4) : 3156-3166 . |
| MLA | Zhang, Junjie et al. "Noninvasive Transdermal Delivery of STING Agonists Reshapes the Immune Microenvironment of Melanoma and Potentiates Checkpoint Blockade Therapy Efficacy" . | ACS Applied Bio Materials 8 . 4 (2025) : 3156-3166 . |
| APA | Zhang, Junjie , Yang, Hui , Li, Liang , Peng, Changkun , Li, Jingying . Noninvasive Transdermal Delivery of STING Agonists Reshapes the Immune Microenvironment of Melanoma and Potentiates Checkpoint Blockade Therapy Efficacy . | ACS Applied Bio Materials , 2025 , 8 (4) , 3156-3166 . |
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Among nucleic acid-based cell membrane functionalization strategies, target recognition is a common route to attach DNA probes on receptors via modifying DNA with receptor-biorecognition elements. Nucleic acid has evolved into a promising molecular tool for probing receptors and even manipulating receptor functions. Given the diversity of receptor-binding aptamers, the covalent chemical conjugation strategies could be easily expanded to other target receptors by using appropriate biorecognition elements. Overall, the nucleic acid-based cell membrane functionalization strategies combined with receptor-biorecognition elements have succeeded in the connection of DNA probes with selective receptors, providing desirable opportunities for cellular receptor visualization and regulation. Cellular receptors offer abundant molecular information closely relevant to cell behaviors, mainly involving receptor expression levels, posttranslational modification patterns, and dynamic nanoscale organizations. © 2024 WILEY-VCH GmbH, BoschstraΒe 12, 69469 Weinheim, Germany.
Keyword ：
cell membrane functionalization strategies cell membrane functionalization strategies cellular receptor regulation cellular receptor regulation cellular receptor visualization cellular receptor visualization DNA probes DNA probes nucleic acid nucleic acid receptor-biorecognition elements receptor-biorecognition elements
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| GB/T 7714 | Chen, S. , Li, J. , Yang, H. . Cell Membrane Functionalization via Nucleic Acid Tools for Visualization and Regulation of Cellular Receptors [未知]. |
| MLA | Chen, S. et al. "Cell Membrane Functionalization via Nucleic Acid Tools for Visualization and Regulation of Cellular Receptors" [未知]. |
| APA | Chen, S. , Li, J. , Yang, H. . Cell Membrane Functionalization via Nucleic Acid Tools for Visualization and Regulation of Cellular Receptors [未知]. |
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Transdermal drug delivery system (TDDS) offers lower systemic toxicity and good patient compliance, making it a promising treatment option for skin-related cancers. However, physiological barriers in the skin frequently impede the therapeutic efficiency of TDDS. To address this, a unique self-assembled TDDS that incorporates disulfide pendant groups (termed Sup-TDDS) is presented. It is formulated with dithiolane-containing lipoic acid (LA), photosensitizers Ce6, and chemotherapeutic agents trametinib. Pendant disulfide moieties on Sup-TDDS facilitate thiol-disulfide exchange reactions with exofacial thiols on cell surfaces, thus enhancing stratum corneum penetration. In contrast to intravenous injection, topical administration of Sup-TDDS can penetrate deeper into the skin (> 500 mu m) and promote drug accumulation in subcutaneous tumors. In a B16F10-bearing mouse model, Sup-TDDS treatment demonstrates significant anti-tumor effects in primary and recurrent melanoma, benefiting from the synergistic effects of Ce6 and trametinib. These results underscore that Sup-TDDS's transdermal properties allow non-invasive melanoma therapy, implying the potential of nanodrugs containing pendant disulfides for transdermal treatment of skin illnesses.
Keyword ：
drug delivery drug delivery melanoma melanoma synergistic therapy synergistic therapy thiol-mediated uptake thiol-mediated uptake transdermal administration transdermal administration
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| GB/T 7714 | Zhang, Junjie , Peng, Changkun , Liu, Shuya et al. A Self-Assembled Transdermal Nanomedicines Incorporating Pendant Disulfides for Non-Invasive, Synergistic Treatment of Melanoma [J]. | ADVANCED HEALTHCARE MATERIALS , 2024 . |
| MLA | Zhang, Junjie et al. "A Self-Assembled Transdermal Nanomedicines Incorporating Pendant Disulfides for Non-Invasive, Synergistic Treatment of Melanoma" . | ADVANCED HEALTHCARE MATERIALS (2024) . |
| APA | Zhang, Junjie , Peng, Changkun , Liu, Shuya , Lian, Zhijie , Zhou, Jie , Li, Jingying et al. A Self-Assembled Transdermal Nanomedicines Incorporating Pendant Disulfides for Non-Invasive, Synergistic Treatment of Melanoma . | ADVANCED HEALTHCARE MATERIALS , 2024 . |
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The first approved RNAi therapeutics, ONPATTRO, in 2017 moves the concept of RNA interference (RNAi) therapy from research to clinical reality, raising the hopes for the treatment of currently incurable diseases. However, RNAi therapeutics are still facing two main challenges-susceptibility to enzymatic degradation and low ability to escape from endo/lysosome into the cytoplasm. Therefore, we developed disulfide-based nanospheres (DBNPs) as universal vehicles to achieve efficient RNA delivery to address these problems. Notably, the DBNPs possess unique and desirable features, including improved resistance to nuclease degradation, direct cytoplasmic delivery through thiol-mediated cellular uptake, and cytosolic environment-responsive release, greatly enhancing the bioavailability of RNA therapeutics. Additionally, DBNPs are superior in terms of overcoming formidable physiological barriers, including vascular barriers and impermeable tumor tissues. Owning to these advantages, the DBNPs exhibit efficient gene silencing effect when delivering either small interfering RNA (siRNA) or microRNA in various cell lines and gener-ate remarkable growth inhibition in the zebrafish and mouse model of pancreatic tumors as compared to traditional delivery vectors, such as PEI. Therefore, DBNPs have potential application prospect in RNAi therapy both in vitro and in vivo.
Keyword ：
Cytoplasmic delivery Cytoplasmic delivery Gene silencing Gene silencing Pancreatic tumor Pancreatic tumor RNAi therapeutics RNAi therapeutics Thiol-mediated uptake Thiol-mediated uptake Tumor permeability Tumor permeability Vascular penetration Vascular penetration Zebrafish Zebrafish
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| GB/T 7714 | Zhou, Jie , Zhang, Junjie , Chen, Senyan et al. Full length article Direct cytoplasmic delivery of RNAi therapeutics through a non-lysosomal pathway for enhanced gene therapy [J]. | ACTA BIOMATERIALIA , 2023 , 170 : 401-414 . |
| MLA | Zhou, Jie et al. "Full length article Direct cytoplasmic delivery of RNAi therapeutics through a non-lysosomal pathway for enhanced gene therapy" . | ACTA BIOMATERIALIA 170 (2023) : 401-414 . |
| APA | Zhou, Jie , Zhang, Junjie , Chen, Senyan , Lin, Qinghua , Zhu, Rong , Wang, Liping et al. Full length article Direct cytoplasmic delivery of RNAi therapeutics through a non-lysosomal pathway for enhanced gene therapy . | ACTA BIOMATERIALIA , 2023 , 170 , 401-414 . |
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We developed a circular bivalent aptamer (CBA) to precisely activate membrane receptor-mediated regenerative signaling for liver repair in vivo. The CBA showed enhanced biostability and receptor binding avidity, achieving effective pathway activation and satisfactory treatment in an acetaminophen-induced liver injury model. This work expands aptamer-based molecular engineering in regenerative medicine.
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| GB/T 7714 | Liang, Hong , Yan, Zhike , Tong, Yuhong et al. Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo [J]. | CHEMICAL COMMUNICATIONS , 2023 , 59 (12) : 1621-1624 . |
| MLA | Liang, Hong et al. "Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo" . | CHEMICAL COMMUNICATIONS 59 . 12 (2023) : 1621-1624 . |
| APA | Liang, Hong , Yan, Zhike , Tong, Yuhong , Chen, Shan , Li, Jingying , Chen, Lanlan et al. Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo . | CHEMICAL COMMUNICATIONS , 2023 , 59 (12) , 1621-1624 . |
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The complexity of genetic circuits has not seen a significant increase over the last decades, even with the rapid development of synthetic biology tools. One of the bottlenecks is the limited number of orthogonal transcription factor-operator pairs. Researchers have tried to use aptamer-ligand pairs as genetic parts to regulate transcription. However, most aptamers selected using traditional methods cannot be directly applied in gene circuits for transcriptional regulation. To that end, we report a new method called CIVT-SELEX to select DNA aptamers that can not only bind to macromolecule ligands but also undergo significant conformational changes, thus affecting transcription. The single-stranded DNA library with affinity to our example ligand human thrombin protein is first selected and enriched. Then, these ssDNAs are inserted into a genetic circuit and tested in the in vitro transcription screening to obtain the ones with significant inhibitory effects on downstream gene transcription when thrombins are present. These aptamer-thrombin pairs can inhibit the transcription of downstream genes, demonstrating the feasibility and robustness of their use as genetic parts in both linear DNAs and plasmids. We believe that this method can be applied to select aptamers of any target ligands and vastly expand the genetic part library for transcriptional regulation.
Keyword ：
aptamer aptamer CIVT-SELEX CIVT-SELEX genetic part genetic part synthetic biology synthetic biology thrombin thrombin transcriptional regulation transcriptional regulation
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| GB/T 7714 | Guo, Shaobin , Xu, Zeqi , Lin, Lujie et al. Using CIVT-SELEX to Select Aptamers as Genetic Parts to Regulate Gene Circuits in a Cell-Free System [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (3) . |
| MLA | Guo, Shaobin et al. "Using CIVT-SELEX to Select Aptamers as Genetic Parts to Regulate Gene Circuits in a Cell-Free System" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 3 (2023) . |
| APA | Guo, Shaobin , Xu, Zeqi , Lin, Lujie , Guo, Yan , Li, Jingying , Lu, Chunhua et al. Using CIVT-SELEX to Select Aptamers as Genetic Parts to Regulate Gene Circuits in a Cell-Free System . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (3) . |
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Abnormal tumor microenvironment imposes barriers to tumor penetration of nanomedicine, which remains a major challenge for effective anti-tumor. Herein, we present disulfide-based nanoparticles that actively penetrate deep tumors in vivo through a thiol-mediated transportation pathway. To achieve active tumor accumulation in vivo, disulfide-based nanoparticles are modified with folic acid units (FA-DBNPs). It is gratifying that FA-DBNPs still enter cells via the thiol-mediated pathway, which facilitates transcellular transportation and tumor penetration both in vitro and in vivo. Besides, FA-DBNPs exhibit GSH concentration-dependent depolymerization characterization, indicating that the GSH level in tumor tissues regulates the penetration depth of FA-DBNPs. Benefiting from these advantages, FA-DBNPs showed potent anti-tumor activity in mouse models, leading to the significant regression of tumors. The current study lays a foundation that thiol-mediated transportation is a promising approach in nanomedicine design for solid tumor therapy.
Keyword ：
deep penetration deep penetration solid tumor solid tumor thiol-mediated pathway thiol-mediated pathway transcellular transportation transcellular transportation
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| GB/T 7714 | Zhang, Junjie , Sun, Weican , Wang, Xing et al. Thiol-mediated transportation pathway: an approach for improving tumor penetration of nanomedicines in vivo [J]. | SCIENCE CHINA-CHEMISTRY , 2023 , 67 (1) : 383-389 . |
| MLA | Zhang, Junjie et al. "Thiol-mediated transportation pathway: an approach for improving tumor penetration of nanomedicines in vivo" . | SCIENCE CHINA-CHEMISTRY 67 . 1 (2023) : 383-389 . |
| APA | Zhang, Junjie , Sun, Weican , Wang, Xing , Chen, Senyan , Li, Jingying , Yang, Huanghao . Thiol-mediated transportation pathway: an approach for improving tumor penetration of nanomedicines in vivo . | SCIENCE CHINA-CHEMISTRY , 2023 , 67 (1) , 383-389 . |
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Transmembrane proteins (TMEMs) are integrated membrane proteins that span the entire lipid bilayer and are permanently anchored to it. TMEMs participate in various cellular processes. Some TMEMs usually exist and perform their physiological functions as dimers rather than monomers. TMEM dimerization is associated with various physiological functions, such as the regulation of enzyme activity, signal transduction, and cancer immunotherapy. In this review, we focus on the dimerization of transmembrane proteins in cancer immunotherapy. This review is divided into three parts. First, the structures and functions of several TMEMs related to tumor immunity are introduced. Second, the characteristics and functions of several typical TMEM dimerization processes are analyzed. Finally, the application of the regulation of TMEM dimerization in cancer immunotherapy is introduced.
Keyword ：
cancer immunotherapy cancer immunotherapy dimerization dimerization dimerization regulation dimerization regulation small-molecule drugs small-molecule drugs transmembrane proteins transmembrane proteins
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| GB/T 7714 | Li, Lei , Li, Jingying . Dimerization of Transmembrane Proteins in Cancer Immunotherapy [J]. | MEMBRANES , 2023 , 13 (4) . |
| MLA | Li, Lei et al. "Dimerization of Transmembrane Proteins in Cancer Immunotherapy" . | MEMBRANES 13 . 4 (2023) . |
| APA | Li, Lei , Li, Jingying . Dimerization of Transmembrane Proteins in Cancer Immunotherapy . | MEMBRANES , 2023 , 13 (4) . |
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Current therapeutic strategies for Alzheimer's disease (AD) mainly focus on amyloid beta oligomer (A beta O) formation or clearance. However, most of them have failed to yield good clinical results. There is an urgent need to explore an alternative therapeutic target for AD treatments. Recent studies have indicated that the cellular prion protein (PrPC) is one of the cell-surface receptors of A beta O that mediates related neurotoxicity. Besides, as a neuroprotective protein, the dimerization of PrPC seems to be critical for its trophic activity. We presume that modulating PrPC receptor activity could be another potential approach to abrogate A beta O toxicity. In the present work, using an aptamer-induced dimerization (AID) strategy, we enforce PrPC dimerization and modulate its neurotrophic signaling. The AID strategy can attenuate A beta O toxic action by (i) interfering with A beta O-PrPC interaction and promoting neuroprotective shedding of PrPC; (ii) preventing the A beta O-induced mitochondrial dysfunction and the caspase-3-induced apoptosis; and (iii) reducing the secretion of inflammatory cytokines and relieving the neuroinflammation microenvironment. Our findings suggest that the strategy targeting PrPC signaling may shed light on validating new therapeutic strategies in AD.
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| GB/T 7714 | Liu, Shuya , Li, Shiwei , Lin, Jirong et al. Aptamer-Induced-Dimerization Strategy Attenuates A beta O Toxicity through Modulating the Trophic Activity of PrPC Signaling [J]. | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 2022 . |
| MLA | Liu, Shuya et al. "Aptamer-Induced-Dimerization Strategy Attenuates A beta O Toxicity through Modulating the Trophic Activity of PrPC Signaling" . | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022) . |
| APA | Liu, Shuya , Li, Shiwei , Lin, Jirong , Li, Jingying , Yang, Huanghao . Aptamer-Induced-Dimerization Strategy Attenuates A beta O Toxicity through Modulating the Trophic Activity of PrPC Signaling . | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 2022 . |
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