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学者姓名：林珍珍
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Abstract ：
高校实验室是科技创新和人才培养的摇篮,科学的实验室管理方式能够充分发挥实验室功能,也是提高教学质量,完成实验任务的根本保障。在结合实验室工作实际情况的基础上,借鉴国内外科研院所实验室建设经验,分析了高校实验室管理工作中的问题和不足,从实验室信息化建设、开放实验室建设、实验室安全建设等方面探讨实验室的管理方法和管理模式。以提高实验室管理效率,更好地服务于教学科研工作,为聚力福州大学“双一流”建设提供实验室支持。
Keyword ：
信息化 信息化 实验室安全 实验室安全 实验平台 实验平台 设备共享 设备共享
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| GB/T 7714 | 何春娴 , 陈星伟 , 林珍珍 et al. 高校实验平台管理模式探索 [J]. | 实验室科学 , 2024 , 27 (01) : 182-184,188 . |
| MLA | 何春娴 et al. "高校实验平台管理模式探索" . | 实验室科学 27 . 01 (2024) : 182-184,188 . |
| APA | 何春娴 , 陈星伟 , 林珍珍 , 丁正新 . 高校实验平台管理模式探索 . | 实验室科学 , 2024 , 27 (01) , 182-184,188 . |
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Molecular tags such as fluorophores are increasingly being replaced with nanoparticles thanks to their superior optical properties, substantial chemical stability, and stability against photobleaching. Herein, we innovatively constructed a new ratiometric fluorescence enzyme-linked immunosorbent assay (RF-ELISA) for the screening of alpha-fetoprotein (AFP) in early hepatocellular carcinoma in vitro diagnostics using carbon dots@SiO2@CdTe quantum dots (CDs@SiO2@CdTe QDs). Carbon dots with blue fluorescence were initially encapsulated into SiO2 nanospheres through the typical Stober method. Thereafter, CdTe QDs with red fluorescence were modified onto the surface of CDs@SiO2 nanospheres. Dual-emission nanotags with blue and red fluorescent signals were utilized to design a RF-ELISA method for the determination of AFP on the anti-AFP capture antibody-coated microplate using glucose oxidase (GOx)-labeled anti-AFP secondary antibody. After the formation of the sandwiched immunocomplex, GOx catalyzed glucose to generate hydrogen peroxide (H2O2), which could quench the red fluorescence of CdTe QDs on the surface of nanotags. Meanwhile, the encapsulated carbon dots in the nanotags could still maintain the initial blue fluorescence intensity. The ratio between red fluorescence intensity and blue-emission intensity could be used for the quantitative monitoring of AFP concentration under optimum conditions. The experimental results indicated that CDs@SiO2@CdTe QDs-based RF-ELISA could exhibit a good fluorescence signal with a dynamic linear range of 0.05-60 ng mL(-1) at a low detection limit of 8.7 pg mL(-1). Moreover, the fluorescence color of the solution including CDs@SiO2@CdTe QDs changed from pink to purple to blue with the increasing AFP level when viewed by the naked eye. Good reproducibility, high specificity, and acceptable stability were achieved for the analysis of target AFP. Importantly, the accuracy of ratiometric fluorescence immunoassay was evaluated to determine human serum samples, giving well-matched results relative to commercially usable human AFP ELISA method.
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| GB/T 7714 | Liang, Xiuhui , Lin, Zhenzhen , Li, Ling et al. Ratiometric fluorescence enzyme-linked immunosorbent assay based on carbon dots@SiO2@CdTe quantum dots with dual functionalities for alpha-fetoprotein [J]. | ANALYST , 2022 , 147 (12) : 2851-2858 . |
| MLA | Liang, Xiuhui et al. "Ratiometric fluorescence enzyme-linked immunosorbent assay based on carbon dots@SiO2@CdTe quantum dots with dual functionalities for alpha-fetoprotein" . | ANALYST 147 . 12 (2022) : 2851-2858 . |
| APA | Liang, Xiuhui , Lin, Zhenzhen , Li, Ling , Tang, Dianping , Kong, Jinfeng . Ratiometric fluorescence enzyme-linked immunosorbent assay based on carbon dots@SiO2@CdTe quantum dots with dual functionalities for alpha-fetoprotein . | ANALYST , 2022 , 147 (12) , 2851-2858 . |
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Abstract ：
介绍了福建省首届大学生化学实验邀请赛物理化学实验的命题思路、试题及评分规则,并对实验中存在的问题及成绩进行了总结分析.
Keyword ：
化学实验邀请赛 化学实验邀请赛 物理化学实验 物理化学实验 福建省 福建省
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| GB/T 7714 | 祝淑颖 , 林珍珍 , 丁开宁 et al. 福建省首届大学生化学实验邀请赛物理化学部分的评析与思考 [J]. | 大学化学 , 2018 , 33 (10) : 114-119 . |
| MLA | 祝淑颖 et al. "福建省首届大学生化学实验邀请赛物理化学部分的评析与思考" . | 大学化学 33 . 10 (2018) : 114-119 . |
| APA | 祝淑颖 , 林珍珍 , 丁开宁 , 李奕 , 汤儆 , 袁耀锋 . 福建省首届大学生化学实验邀请赛物理化学部分的评析与思考 . | 大学化学 , 2018 , 33 (10) , 114-119 . |
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Methods based on photoelectrochemistry have been developed for immunoassay, but most involve in a low sensitivity and a relatively narrow detectable range. Herein a new bio-bar-code-based split-type photoelectrochemical (PEC) immunoassay was designed for sensitive detection of prostate-specific antigen (PSA), coupling rolling circle amplification (RCA) with enzymatic biocatalytic precipitation. The bio-bar-code-based immunoreaction was carried out on monoclonal anti-PSA antibody (mAb(1))-coated microplate using primer DNA and polyclonal anti-PSA antibody-conjugated gold nanoparticle (pDNA-AuNP-pAb(2)) with a sandwich-type assay format. Accompanying the immunocomplex, the labeled primer DNA on gold nanoparticle readily triggered RCA reaction in the presence of padlock probe/dNTPs/ligase/polymerase. The RCA product with a long single stranded DNA could cause the formation of numerous hemin/G-quadruplex-based DNAzyme concatamers. With the assistance of nicking endonuclease, DNAzyme concatamers were dissociated from gold nanoparticle, which catalyzed the precipitation of 4-chloro-l-naphthol in the presence of H2O2 onto CdS nanorods-coated electrode (as the photoanode for the generated holes). The formed insoluble precipitate inhibited the electron transfer from the solution to CdS nanorods-modified electrode by using ascorbic acid as the electron donor. Under the optimum conditions, the photocurrent of the modified electrode decreased with the increasing of PSA concentration. A detectable concentration for target PSA with this system could be achieved as low as 1.8 pg mL(-1) In addition, our strategy also showed good reproducibility, high specificity and accuracy matched well with commercial PSA ELISA kits for real sample analysis. These remarkable properties revealed that the developed PEC immunoassay has great potential as a useful tool for the detection of PSA in practical application.
Keyword ：
CdS nanorods CdS nanorods DNAzyme concatamers DNAzyme concatamers Enzymatic biocatalytic precipitation Enzymatic biocatalytic precipitation Photoelectrochemical immunosensor Photoelectrochemical immunosensor Rolling circle amplification Rolling circle amplification
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| GB/T 7714 | Zhang, Kangyao , Lv, Shuzhen , Lin, Zhenzhen et al. Bio-bar-code-based photoelectrochemical immunoassay for sensitive detection of prostate-specific antigen using rolling circle amplification and enzymatic biocatalytic precipitation [J]. | BIOSENSORS & BIOELECTRONICS , 2018 , 101 : 159-166 . |
| MLA | Zhang, Kangyao et al. "Bio-bar-code-based photoelectrochemical immunoassay for sensitive detection of prostate-specific antigen using rolling circle amplification and enzymatic biocatalytic precipitation" . | BIOSENSORS & BIOELECTRONICS 101 (2018) : 159-166 . |
| APA | Zhang, Kangyao , Lv, Shuzhen , Lin, Zhenzhen , Li, Meijin , Tang, Dianping . Bio-bar-code-based photoelectrochemical immunoassay for sensitive detection of prostate-specific antigen using rolling circle amplification and enzymatic biocatalytic precipitation . | BIOSENSORS & BIOELECTRONICS , 2018 , 101 , 159-166 . |
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This work designs a simple low-cost visual fluorescence immunoassay for disease-related biomarkers (carcinoembryonic antigen, CEA, used as a model protein) in biological fluids, based on the structural and optical transformation of CdTe quantum dots (QDs) immobilized on paper induced by dissolved silver ions (Ag+) from silver nanoparticles (AgNPs) via a cation-exchange reaction. A sandwich-type immunoreaction was initially carried out in a removable polystyrene high-binding microplate coated with monoclonal antiCEA capture antibody by using AgNP-labeled polyclonal anti-CEA antibody as the detection antibody. Thereafter, the carried AgNPs accompanying the sandwiched immunocomplexes were dissolved by acid to release numerous silver ions, which induced the ion-exchange reaction with the immobilized CdTe QDs on the paper (attached onto the microplate lid) for the Cd-to-Ag transformation, thus resulting in the quenching of the visual fluorescence from the CdTe QDs owing to Ag2Te formation. Under optimal conditions, the fluorescence intensity decreased with the increasing CEA concentration from 0.02 to 50 ng mL(-1) with a detection limit of 5.6 pg mL(-1). Further, a visual assay based on a CdTe QD-based paper sensor was developed for CEA detection, and 5.0 pg mL(-1) CEA could be discriminated with the naked eye. In addition, our strategy displayed high specificity, good reproducibility and acceptable accuracy for analyzing human serum specimens with consistent results obtained using the commercialized enzyme-linked immunosorbent assay (ELISA) method.
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| GB/T 7714 | Lin, Zhenzhen , Lv, Shuzhen , Zhang, Kangyao et al. Optical transformation of a CdTe quantum dot-based paper sensor for a visual fluorescence immunoassay induced by dissolved silver ions [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2017 , 5 (4) : 826-833 . |
| MLA | Lin, Zhenzhen et al. "Optical transformation of a CdTe quantum dot-based paper sensor for a visual fluorescence immunoassay induced by dissolved silver ions" . | JOURNAL OF MATERIALS CHEMISTRY B 5 . 4 (2017) : 826-833 . |
| APA | Lin, Zhenzhen , Lv, Shuzhen , Zhang, Kangyao , Tang, Dianping . Optical transformation of a CdTe quantum dot-based paper sensor for a visual fluorescence immunoassay induced by dissolved silver ions . | JOURNAL OF MATERIALS CHEMISTRY B , 2017 , 5 (4) , 826-833 . |
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Methods based on nanostructures have been developed for potentiometric immunosensors, but most involve low sensitivity or weak signal output and are unsuitable for routine use in diagnosis. Herein, we devise an in-situ signal-amplification strategy for enhanced electrical readout of potentiometric immunosensor toward target prostate-specific antigen (PSA, one kind of cancer biomarkers), based on polyion oligonucleotide- labeled gold nanoparticles (AuNPs). To decrease the background signal, monoclonal antihuman PSA capture antibody was covalently conjugated onto an activated glassy carbon electrode via typical carbodiimide coupling. AuNPs heavily functionalized with the polyion oligonucleotides and polyclonal anti- PSA detection antibodies (pAb(2)-AuNP-DNA) were utilized as the signal-generation nanotags. In the presence of target PSA, a sandwich-type immunoreaction was executed between capture antibody and detection antibody on the electrode. The detectable signal derived from the shift in the electric potential as a result of the change in the surface charge before and after the antigen-antibody reaction. With target PSA increased, the captured pAb(2)-AuNP-DNA to the electrode accompanying detection antibody increased, thereby resulting in the change of the electrode potential. Due to numerous polyion oligonucleotides with the negative charge, the signal readout amplified. Under the optimal conditions, the shift in the output potential was proportional to the logarithm of target PSA concentration and displayed a dynamic linear range from 0.05 to 20 ng mL(-1) with a detection limit of 13.6 pg mL(-1). An intermediate precision of <= 13.2% was accomplished with the batch-to-batch identification. The selectivity was acceptable. The method accuracy was evaluated for human serum specimens, and gave the consistent results between the potentiometric immunosensor and the referenced enzymelinked immunosorbent assay (ELISA). (C) 2017 Elsevier B. V. All rights reserved.
Keyword ：
Gold nanoparticles Gold nanoparticles Polyion oligonucleotides Polyion oligonucleotides Potentiometric immunosensor Potentiometric immunosensor Prostate-specific antigen Prostate-specific antigen Signal-amplification strategy Signal-amplification strategy
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| GB/T 7714 | Lv, Shuzhen , Lin, Zhenzhen , Zhang, Kangyao et al. Polyion oligonucleotide-decorated gold nanoparticles with tunable surface charge density for amplified signal output of potentiometric immunosensor [J]. | ANALYTICA CHIMICA ACTA , 2017 , 964 : 67-73 . |
| MLA | Lv, Shuzhen et al. "Polyion oligonucleotide-decorated gold nanoparticles with tunable surface charge density for amplified signal output of potentiometric immunosensor" . | ANALYTICA CHIMICA ACTA 964 (2017) : 67-73 . |
| APA | Lv, Shuzhen , Lin, Zhenzhen , Zhang, Kangyao , Lu, Minghua , Tang, Dianping . Polyion oligonucleotide-decorated gold nanoparticles with tunable surface charge density for amplified signal output of potentiometric immunosensor . | ANALYTICA CHIMICA ACTA , 2017 , 964 , 67-73 . |
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A novel p-type semiconductor material (p-CuBi2O4) is designed for the construction of split-type photoelectrochemical (PEC) immunosensor for alpha-fetoprotein (AFP) with the hemin assistant to enhance the cathodic photocurrent. Initially, the photocathode of PEC immunosensor is fabricated by p-CuBi2O4 on a layer of gold nanoparticles (AuNPs, as a front contact of p-CuBi2O4) to enhance the efficiency of charge separation. In the presence of target AFP, a sandwich-type immunoreaction was carried out in capture antibody-coated microplate by using detection antibody and hemin-based G-quadruplex (labeled on the AuNP) as the signal probe. Upon exonuclease I (Exo I) introduction, the enzyme digested the hemin/ G-quadruplex-based DNAzyme to release the hemin[Fe(III)], which captured the generated electrons of p-CuBi2O4-based photocathode to enhance photocurrent via the reduction of hemin[Fe(III)] to hemin[Fe(II)] in PEC detection system. Under the optimal conditions, the split-type photocathodic immunosensor showed a wide linear dynamic range from 50 pg mL(-1) to 20 ng mL(-1) at a limit of detection (LOD) of 14.7 pg mL(-1) toward target AFP. Moreover, the PEC immunosensor also displayed high specificity and good reproducibility. Favorably, method accuracy was evaluated to analyze human serum specimens, and gave matched-well results in comparison with commercially available enzyme-linked immunosorbent assay (ELISA) method.
Keyword ：
Disease-related biomarker Disease-related biomarker Exonuclease I Exonuclease I Hemin/G-quadruplex-based DNAzyme Hemin/G-quadruplex-based DNAzyme p-type CuBi2O4 photocathodic material p-type CuBi2O4 photocathodic material Split-type photoelectrochemical immunosensor Split-type photoelectrochemical immunosensor
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| GB/T 7714 | Lv, Shuzhen , Zhang, Kangyao , Lin, Zhenzhen et al. Novel photoelectrochemical immunosensor for disease-related protein assisted by hemin/G-quadruplex-based DNAzyme on gold nanoparticles to enhance cathodic photocurrent on p-CuBi2O4 semiconductor [J]. | BIOSENSORS & BIOELECTRONICS , 2017 , 96 : 317-323 . |
| MLA | Lv, Shuzhen et al. "Novel photoelectrochemical immunosensor for disease-related protein assisted by hemin/G-quadruplex-based DNAzyme on gold nanoparticles to enhance cathodic photocurrent on p-CuBi2O4 semiconductor" . | BIOSENSORS & BIOELECTRONICS 96 (2017) : 317-323 . |
| APA | Lv, Shuzhen , Zhang, Kangyao , Lin, Zhenzhen , Tang, Dianping . Novel photoelectrochemical immunosensor for disease-related protein assisted by hemin/G-quadruplex-based DNAzyme on gold nanoparticles to enhance cathodic photocurrent on p-CuBi2O4 semiconductor . | BIOSENSORS & BIOELECTRONICS , 2017 , 96 , 317-323 . |
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Herein, a novel immunosensing strategy was designed for the sensitive fluorescence/visual detection of alpha-fetoprotein (AFP), by combining the enzyme-controlled formation of polydopamine (PDA) nanoparticles with the dissolution of manganese dioxide (MnO2) nanoflakes. Initially, a sandwich-type immunoreaction was fabricated on a capture antibody-coated microplate by using detection antibody/ascorbate oxidase-conjugating gold nanoparticles (AOX-AuNP-Ab(2)) as the detection probe. The ascorbate oxidase, carried within the immunocomplex, oxidized ascorbic acid (AA) to dehydroascorbic acid (DAA) to inhibit the dissolution of the MnO2 nanoflakes. This resulted in an increment in fluorescent PDA nanoparticles from the oxidation of dopamine (DA) into its quinone derivative with the aid of the MnO2 nanoflakes. Under optimal conditions, the fluorescence intensity of the PDA nanoparticles increased with increasing AFP concentration within the dynamic working range of 0.05-20 ng mL(-1), with a detection limit (LOD) of 17.3 pg mL(-1). The sensor also showed high specificity and acceptable accuracy for the detection of target AFP against other potential cancer biomarkers. Importantly, this study demonstrated for the first time that fluorescent PDA nanoparticles could be used as signal-generation tags and applied to an enzyme immunoassay. Although this methodology focused on AFP detection, it would be suitable for the detection of other cancer biomarkers if the corresponding antibodies were employed.
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| GB/T 7714 | Lin, Zhenzhen , Li, Meijin , Lv, Shuzhen et al. In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for a sensitive immunoassay of cancer biomarkers [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2017 , 5 (43) : 8506-8513 . |
| MLA | Lin, Zhenzhen et al. "In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for a sensitive immunoassay of cancer biomarkers" . | JOURNAL OF MATERIALS CHEMISTRY B 5 . 43 (2017) : 8506-8513 . |
| APA | Lin, Zhenzhen , Li, Meijin , Lv, Shuzhen , Zhang, Kangyao , Lu, Minghua , Tang, Dianping . In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for a sensitive immunoassay of cancer biomarkers . | JOURNAL OF MATERIALS CHEMISTRY B , 2017 , 5 (43) , 8506-8513 . |
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A class of 0-dimensional/2-dimensional (0D/2D) nanoheterostructures based on carbon quantum dots (CQDs) and graphitic carbon nitride (g-C3N4) was designed as the signal-generation tags for the sensitive photoelectrochemical (PEC) immunoassay of prostate-specific antigen (PSA) coupling with the copper nanoclusters (CuNCs). Combination of CQDs with g-C3N4 promoted the photoexcited electron/hole separation and largely increased the photocurrents of the nanoheterostructures. Initially, a sandwich-type immunoreaction was carried out on monoclonal anti-PSA antibody-coated microplate by using PSA aptamer linked with CuNCs as the tracer. Accompanying the immunocomplex, the carried CuNCs were dissolved under acidic conditions. The as-released copper ions from the CuNCs could be captured onto the CQDs/g-C3N4 nanoheterostructures via the amino-group on the CQD surface as well as the -NHx (x = 1, 2, 3) of g-C3N4 nanosheets. The strong coordination of the Lewis basic sites on the CQDs/g-C3N4 with Cu2+ decreased the photocurrent of the nanoheterostructures. Under optimal conditions, CQDs/g-C3N4 nanoheterostructures displayed good photocurrent responses for the detection of PSA within the dynamic linear range of 0.02-100 ng mL(-1) and a limit of detection (LOD) of 5.0 pg mL(-1). This method was also evaluated for quantitative screening of human PSA serum specimens by using the referenced electrochemiluminescent enzyme-linked immunoassay (ECL-ELIA) and gave good matched results between two methods. Additionally, this system was beneficial to explore the charge-separation and photoinduced electron transfer mechanism in the photoelectrochemical sensing protocols.
Keyword ：
carbon quantum dots carbon quantum dots copper nanoclusters copper nanoclusters graphitic carbon nitride graphitic carbon nitride nanoheterostructures nanoheterostructures photoelectrochemical immunoassay photoelectrochemical immunoassay
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| GB/T 7714 | Lv, Shuzhen , Li, Yi , Zhang, Kangyao et al. Carbon Dots/g-C3N4 Nanoheterostructures-Based Signal-Generation Tags for Photoelectrochemical Immunoassay of Cancer Biomarkers Coupling with Copper Nanoclusters [J]. | ACS APPLIED MATERIALS & INTERFACES , 2017 , 9 (44) : 38336-38343 . |
| MLA | Lv, Shuzhen et al. "Carbon Dots/g-C3N4 Nanoheterostructures-Based Signal-Generation Tags for Photoelectrochemical Immunoassay of Cancer Biomarkers Coupling with Copper Nanoclusters" . | ACS APPLIED MATERIALS & INTERFACES 9 . 44 (2017) : 38336-38343 . |
| APA | Lv, Shuzhen , Li, Yi , Zhang, Kangyao , Lin, Zhenzhen , Tang, Dianping . Carbon Dots/g-C3N4 Nanoheterostructures-Based Signal-Generation Tags for Photoelectrochemical Immunoassay of Cancer Biomarkers Coupling with Copper Nanoclusters . | ACS APPLIED MATERIALS & INTERFACES , 2017 , 9 (44) , 38336-38343 . |
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Lysozyme with a small monomeric globular enzymatic protein is part of the innate immune system, and its deficiency can cause the increased incidence of disease. Herein, we devise a new signal-enhanced fluorescence aptasensing platform for quantitative screening of lysozyme by coupling with rolling circle amplification (RCA) and strand hybridization reaction, accompanying the assembly of CdTe/CdSe quantum dots (QDs) and hemin/G-quadruplex DNzyme. Initially, target triggered release of the primer was carried out from DNA duplex via the reaction of the aptamer with the analyte, and the released primer could be then utilized as the template to produce numerous repeated oligonucleotide sequences by the RCA reaction. Following that, the formed long-stranded DNA simultaneously hybridized with the CdTe/CdSe QD-labeled probe and hemin/G-quadruplex DNzyme strand in the system, thereby resulting in the quenching of QD fluorescent signal through the proximity hemin/G-quadruplex DNzyme on the basis of transferring photoexcited conduction band electrons of quantum dots to Fe(III)/Fe(II)-protoporphyrin IX (hemin) complex. Under optimal conditions, the fluorescent signal decreased with the increasing target lysozyme within the dynamic range from 5.0 to 500 nM with a detection limit (LOD) of 2.6 nM at the (3s)biank criterion. Intra-assay and interassay coefficients of variation (CVs) were below 8.5% and 11.5%, respectively. Finally, the system was applied to analyze spiked human serum samples, and the recoveries in all cases were 85-111.9%. (C) 2016 Elsevier B.V. All rights reserved.
Keyword ：
CdTe/CdSe quantum dots CdTe/CdSe quantum dots Fluorescent aptasensor Fluorescent aptasensor Hemin/G-quadruplex DNzyme Hemin/G-quadruplex DNzyme Lysozyme Lysozyme Rolling circle amplification Rolling circle amplification
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| GB/T 7714 | Qiu, Zhenli , Shu, Jian , He, Yu et al. CdTe/CdSe quantum dot-based fluorescent aptasensor with hemin/G-quadruplex DNzyme for sensitive detection of lysozyme using rolling circle amplification and strand hybridization [J]. | BIOSENSORS & BIOELECTRONICS , 2017 , 87 : 18-24 . |
| MLA | Qiu, Zhenli et al. "CdTe/CdSe quantum dot-based fluorescent aptasensor with hemin/G-quadruplex DNzyme for sensitive detection of lysozyme using rolling circle amplification and strand hybridization" . | BIOSENSORS & BIOELECTRONICS 87 (2017) : 18-24 . |
| APA | Qiu, Zhenli , Shu, Jian , He, Yu , Lin, Zhenzhen , Zhang, Kangyao , Lv, Shuzhen et al. CdTe/CdSe quantum dot-based fluorescent aptasensor with hemin/G-quadruplex DNzyme for sensitive detection of lysozyme using rolling circle amplification and strand hybridization . | BIOSENSORS & BIOELECTRONICS , 2017 , 87 , 18-24 . |
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