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学者姓名：谢友坪
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Abstract ：
为探索雨生红球藻渣多糖的综合利用价值，本研究采用40%、60%和80%的乙醇梯度分离雨生红球藻渣多糖，依次得到HP40、HP60、HP80三个粗多糖组分，通过高效液相色谱（HPLC）、傅里叶红外光谱（FT-IR）、凝胶色谱-示差-多角度激光光散射系统（HPSEC-MALS-RI）等方法对各组分多糖的单糖组成、分子量、结构、粒径和Zeta电位等理化特性进行分析，并测定其抗氧化活性。结果表明，乙醇浓度显著影响所沉淀雨生红球藻渣多糖的理化性质和抗氧化活性。醇沉粗多糖的得率和纯度随着乙醇浓度的增加而降低，并且乙醇浓度越高，沉淀的粗多糖分子量越低。本研究提取的粗多糖的纯度较高，其中HP40的总糖含量可达99.46%±0.015%。三种组分均包含Glc、Man、Gal、Ara、Rha、Xyl、Fuc、Glc-UA这8种单糖，但单糖组成及比例并不相同，与HP40相比，HP60和HP80还含有核糖，共有9种单糖。HP的粒径和Zeta电位分析结果表明，HP80的粒径最小（73.505±2.405 nm）且Zeta电位的绝对值最大（−11.6 mV）。抗氧化分析结果表明，HP80的抗氧化活性最强，且对羟自由基的清除率最高可达96%以上（10.0 mg/mL）。综上所述，梯度醇沉可以对雨生红球藻渣多糖进行初步分离，得到单糖组成、粒径、分子量、抗氧化活性各不相同的粗多糖。其中，40%乙醇所沉淀的粗多糖得率和糖含量最高，80%乙醇所沉淀的粗多糖分子量最小、抗氧化能力最强。
Keyword ：
分子量 分子量 单糖组成 单糖组成 多糖 多糖 抗氧化活性 抗氧化活性 梯度醇沉 梯度醇沉 理化性质 理化性质 粒径 粒径 雨生红球藻渣 雨生红球藻渣
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| GB/T 7714 | 肖金艳 , 宋瑞萍 , 王宝贝 et al. 梯度醇沉雨生红球藻渣多糖的理化性质及 体外抗氧化活性研究 [J]. | 食品工业科技 , 2025 , 46 (6) : 1-11 . |
| MLA | 肖金艳 et al. "梯度醇沉雨生红球藻渣多糖的理化性质及 体外抗氧化活性研究" . | 食品工业科技 46 . 6 (2025) : 1-11 . |
| APA | 肖金艳 , 宋瑞萍 , 王宝贝 , 林丽芹 , 谢友坪 , 加晶 . 梯度醇沉雨生红球藻渣多糖的理化性质及 体外抗氧化活性研究 . | 食品工业科技 , 2025 , 46 (6) , 1-11 . |
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Chlorella meal is considered a promising alternative to fish meal in aquafeeds, but the effects of incorporating Chlorella meal as a feed ingredient on the growth and quality of eel have not been explored. The effects of partially substituting fish meal (0 %, 5 %, 10 %, 15 %, and 20 %) with Chlorella meal on the growth performance, whole-body composition, pigmentation, and physiological health of marbled eel (Anguilla marmorata) were evaluated in this study. The results revealed that dietary inclusion of Chlorella meal significantly increased the final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR), but it significantly decreased the feed conversion rate (FCR), with these parameters exhibiting linear trends (P < 0.05). The whole-body protein and fat contents were significantly higher in all groups fed with diets containing Chlorella meal compared to the control group (P < 0.05). In addition, the dorsal and abdominal skin of marbled eel exhibited an orange-yellow color, and the color intensity increased with increasing levels of dietary Chlorella meal. The color change corresponded to increased values of a* (redness), b* (yellowness), and lutein content in the fish skin (P < 0.05). The physiological health of marbled eel also improved in the groups where Chlorella meal was included. The optimal replacement rate was approximately 10 % (9 % Chlorella meal supplementation), because the whole-body protein content and most physiological health parameters were highest under this condition, and the growth performance did not show a significant increase when the fish meal replacement rate exceeded 10 % (P > 0.05). These findings indicate the potential of Chlorella meal as a promising alternative to fish meal in feed formulations, offering benefits in enhancing growth performance and quality of marbled eel.
Keyword ：
Chlorella meal Chlorella meal Growth performance Growth performance Marbled eel Marbled eel Physiological health Physiological health Pigmentation Pigmentation Whole-body composition Whole-body composition
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| GB/T 7714 | Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 . |
| MLA | Huang, Kaiyun et al. "Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata )" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 80 (2024) . |
| APA | Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 . |
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To achieve the high-value utilization of squid skin obtained from squid processing, this study utilized an enzymatic approach to prepare protein peptides. The enzymatic hydrolysis parameters were optimized using single factor and response surface tests, with the concentration of peptide and trichloroacetic acid-nitrogen soluble index (TCA-NSI) serving as evaluation criteria. The amino acid composition, molecular weight distribution, peptide sequence, antioxidant activity, and in vitro simulated digestive properties of the prepared protein peptides were also thoroughly characterized. The results showed that the optimal conditions for enzymatic hydrolysis were as follows: Enzymatic temperature of 43.50 ℃, enzymatic time of 100 minutes, enzymatic pH of 7.50, trypsin to alkaline protease ratio of 2:1, and enzyme dosage of 4000 U/g. Under these specified conditions, the TCA-NSI achieved a value of 89.02%±0.66%. The prepared protein peptides exhibited a well-balanced amino acid composition, with an essential amino acid index (EAAI) value of 0.90. Furthermore, the prepared protein peptides encompassed a total of 43 polypeptide sequences, with 38 of these segments exhibiting molecular weights below 1800 Da. The IC50 values for scavenging DPPH radical, ABTS+ radical, and hydroxyl radical were determined to be 0.61, 0.28 and 1.95 mg/mL, respectively. Notably, the antioxidant activity remained favorable even after undergoing simulated gastrointestinal digestion in vitro. These findings serve to establish a solid theoretical basis for the advancement and exploitation of byproducts derived from squid processing, as well as their subsequent value-added utilization. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.
Keyword ：
Alkalinity Alkalinity Amino acids Amino acids Antioxidants Antioxidants Enzymatic hydrolysis Enzymatic hydrolysis Molecular weight distribution Molecular weight distribution Peptides Peptides Physicochemical properties Physicochemical properties Shellfish Shellfish Trichloroacetic acid Trichloroacetic acid
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| GB/T 7714 | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen et al. Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties [J]. | Science and Technology of Food Industry , 2024 , 45 (7) : 217-226 . |
| MLA | Qingqing, Zhu et al. "Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties" . | Science and Technology of Food Industry 45 . 7 (2024) : 217-226 . |
| APA | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen , Youping, Xie . Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties . | Science and Technology of Food Industry , 2024 , 45 (7) , 217-226 . |
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为实现鱿鱼加工下脚料鱿鱼皮的高值化利用，本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数（Trichloroacetic acid-nitrogen soluble index，TCA-NSI）为评价指标，采用单因素和响应面试验优化酶解工艺参数，并对所制备蛋白肽的氨基酸组成、分子量分布、蛋白肽序列、抗氧化活性及其体外模拟消化特性进行表征。结果表明，最佳酶解工艺条件为酶解温度43.50 ℃、酶解时间100 min、酶解pH7.50、胰蛋白酶与碱性蛋白酶比例2:1、加酶量4000 U/g，在此条件下其TCA-NSI可达89.02%±0.66%。所制备的鱿鱼皮蛋白肽其氨基酸组成均衡，必需氨基酸指数（Essential amino acid index，EAAI）可达0.90。此外，鱿鱼皮蛋白肽含有43个多肽序列，其中38个肽段的分子质量低于1800 Da，其DPPH自由基、ABTS+自由基和羟自由基清除率的IC50分别为0.61、0.28和1.95 mg/mL，且经体外模拟胃肠消化后仍能维持较好的抗氧化活性。本研究结果为鱿鱼加工下脚料的开发利用和精深加工提供了理论依据。
Keyword ：
体外模拟消化 体外模拟消化 抗氧化活性 抗氧化活性 理化性质 理化性质 蛋白肽 蛋白肽 鱿鱼皮 鱿鱼皮
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| GB/T 7714 | 朱清清 , 马瑞娟 , 陈剑锋 et al. 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 [J]. | 食品工业科技 , 2024 : 1-10 . |
| MLA | 朱清清 et al. "响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析" . | 食品工业科技 (2024) : 1-10 . |
| APA | 朱清清 , 马瑞娟 , 陈剑锋 , 谢友坪 . 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 . | 食品工业科技 , 2024 , 1-10 . |
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叶黄素是天然的抗氧化剂,对人体健康有多种益处,异养小球藻具有叶黄素纯度和产量均较高的优势,而小球藻叶黄素产量主要取决于生物质产量和叶黄素含量两个因素.传统的光密度法测生物质产量和高效液相色谱法测叶黄素含量存在操作复杂、时效性低等不足.为了快速、无损测定小球藻生长过程中叶黄素含量变化,搭建可见-近红外双模式快照式多光谱成像检测系统,根据光谱响应区域,分别利用可见光相机获取叶黄素光谱信息,近红外相机获取生物质光谱信息,构建含有生物质量和叶黄素含量信息的可见-近红外双模式多光谱数据集.针对系统所使用的快照式多光谱相机光谱范围宽、波长数量少的特征波长选取问题,提出一种结合序列浮动前向选择的改进型连续投影算法(mSPA);将mSPA与常规的连续投影算法、遗传算法及随机蛙跳三种波长选择算法作对比分析后,构建了基于特征波长的多元线性回归和极限学习机模型;最后,利用生物质产量和叶黄素含量的最佳预测模型生成小球藻叶黄素产量的可视化分布图.结果表明,在利用近红外、可见光相机分别检测小球藻生物质、叶黄素量时,mSPA得到的特征波长数均较少,并具有最高的预测精度.生物质量与叶黄素含量的最佳模型均为mSPA筛选特征波长后建立的极限学习机模型,对应的预测集决定系数分别为0.947和0.907,预测集均方根误差分别为0.698 g·L-1和0.077 mg·g-1,剩余预测偏差分别为3.535和3.338,模型的预测能力较好.可视化分布实现了直观监测小球藻叶黄素产量的变化,有助于后续实际生产中在线检测叶黄素产量.mSPA在快照式多光谱检测小球藻生物质含量及叶黄素含量中,通过对排序波长逐个评估以选择出最佳特征波长组合,有效地避免了特征波长的错选、漏选,提高了模型的预测精度,为快照式多光谱成像技术应用提供新的波长选择思路.
Keyword ：
叶黄素产量 叶黄素产量 小球藻 小球藻 快照式多光谱 快照式多光谱 特征波长 特征波长
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| GB/T 7714 | 沈英 , 占秀兴 , 黄春红 et al. 基于快照式多光谱特征波长的小球藻叶黄素产量快速测定 [J]. | 光谱学与光谱分析 , 2024 , 44 (8) : 2216-2223 . |
| MLA | 沈英 et al. "基于快照式多光谱特征波长的小球藻叶黄素产量快速测定" . | 光谱学与光谱分析 44 . 8 (2024) : 2216-2223 . |
| APA | 沈英 , 占秀兴 , 黄春红 , 谢友坪 , 郭翠霞 , 黄峰 . 基于快照式多光谱特征波长的小球藻叶黄素产量快速测定 . | 光谱学与光谱分析 , 2024 , 44 (8) , 2216-2223 . |
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Nitrogen starvation is a high-efficiency strategy for inducing microalgae to accumulate lipids, polysaccharides, and carotenoids. Traditional nitrogen starvation that transfers microalgal cells into nitrogen depletion abruptly (AND) is labor-consuming and irreversibly damages microalgae. This study used gradual nitrogen depletion in medium (GND) to effectively induce carbohydrates, TAGs, and carotenoids accumulation in Nannochloropsis. After GND for 7 days, the carbohydrate and lipid contents were 1.34- and 0.15-fold higher than the initial content. In addition, astaxnathin and canthaxanthin contents were induced from 0 to 0.32 ± 0.03 mg/g DW and 0.09 ± 0.01 mg/g DW, respectively. Three days of GND revealed 158 significant differentially regulated proteins, including 113 up- and 45 down-regulated proteins. The proteins were involved in pathways for photosynthesis, carbon metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle, pentose phosphate pathway, and fatty acid biosynthesis. Gradual nitrogen depletion stimulated carbohydrate and TAG biosynthesis, fluxed more photosynthetic carbon into the carbohydrate reservoir and strengthened the TCA cycle and glyoxylate cycle. Transcriptional studies also showed that GND up-regulated the genes involved in carbohydrates and TAG biosynthesis. This study proposed a possible GND regulatory model for carbon repartition in Nannochloropsis. © 2024 Elsevier B.V.
Keyword ：
Carbon repartition Carbon repartition Gradual nitrogen depletion Gradual nitrogen depletion Nannochloropsis Nannochloropsis Nitrogen starvation Nitrogen starvation Proteomics Proteomics
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| GB/T 7714 | Wang, B. , Xu, Y. , Lin, L. et al. Remodeling of central carbon metabolism of Nannochloropsis oceanica in response to gradual nitrogen depletion [J]. | Algal Research , 2024 , 82 . |
| MLA | Wang, B. et al. "Remodeling of central carbon metabolism of Nannochloropsis oceanica in response to gradual nitrogen depletion" . | Algal Research 82 (2024) . |
| APA | Wang, B. , Xu, Y. , Lin, L. , Xiao, J. , Xie, Y. , Dai, C. et al. Remodeling of central carbon metabolism of Nannochloropsis oceanica in response to gradual nitrogen depletion . | Algal Research , 2024 , 82 . |
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Microalgae can accumulate various compounds simultaneously, thus co-production of high-value metabolites to improve the economic value of microalgae production is feasible. In this study, a microalga strain Nitzschia sp. FZU62 capable of co-producing fucoxanthin and eicosapentaenoic acid (EPA) was isolated and identified. Further, heterotrophic conditions were optimized to improve the co-production efficiency in flasks. Results showed that the optimal conditions for flask culture were cultivation for 84 h with an inoculum size of 0.47 g/L and a temperature of 19 degrees C. Besides, three fed-batch culture strategies with distinct nutrient compositions were explored to improve the heterotrophic performance of strain FZU62. Results indicated that the highest biomass, fucoxanthin, and EPA productivities were obtained by Fed-batch III, in which concentrated medium was pulse -fed to supply 60 % initial nutrients once glucose concentration dropped to about 2 g/L. Moreover, the hetero-trophic performance was verified in a 5 L fermenter, resulting in excellent fucoxanthin and EPA productions of 63.03 and 398.60 mg/L, and productivities of 3.41 and 22.52 mg/L/d, respectively. These results, especially the EPA production and productivity, are superior to many literature reports. The present study provides new in-sights into the highly efficient co-production of fucoxanthin and EPA by marine microalgae and has guiding significance for commercial production in heterotrophic mode.
Keyword ：
Co -production Co -production Eicosapentaenoic acid Eicosapentaenoic acid Fucoxanthin Fucoxanthin Heterotrophic cultivation Heterotrophic cultivation Microalgae Microalgae
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| GB/T 7714 | Ma, Ruijuan , You, Yuanwei , Liu, Xinyu et al. Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 . |
| MLA | Ma, Ruijuan et al. "Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 71 (2023) . |
| APA | Ma, Ruijuan , You, Yuanwei , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping , Chen, Jianfeng . Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 . |
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BackgroundChlorella sorokiniana FZU60 is a promising lutein producing microalga. A mixotrophy/photoautotrophy two-stage strategy can achieve high biomass concentration at stage 1 and high lutein content at stage 2, leading to excellent lutein production efficiency in C. sorokiniana FZU60. However, the underlying molecular mechanisms are still unclear, restraining the further improvement of lutein production.ResultsIn this study, physiological and biochemical analysis revealed that photochemical parameters (Fv/Fm and NPQ) and photosynthetic pigments contents increased during the shift from mixotrophy to photoautotrophy, indicating that photosynthesis and photoprotection enhanced. Furthermore, transcriptomic analysis revealed that the glyoxylate cycle and TCA cycle were suppressed after the shift to photoautotrophy, leading to a decreased cell growth rate. However, the gene expression levels of photosynthesis, CO2 fixation, autophagy, and lutein biosynthesis were upregulated at the photoautotrophy stage, demonstrating that microalgal cells could obtain more precursor to synthesize lutein for enhancing photosynthesis and reducing reactive oxygen species.ConclusionsThe findings help to elucidate the molecular mechanisms for high lutein production efficiency of C. sorokiniana FZU60 under the mixotrophy/photoautotrophy strategy, identify key functional genes responsible for lutein biosynthesis, and shed light on further improvement of lutein production by genetic or metabolic engineering in future studies.
Keyword ：
Chlorella sorokiniana FZU60 Chlorella sorokiniana FZU60 Lutein Lutein Mixotrophy Mixotrophy Molecular mechanisms Molecular mechanisms Photoautotrophy Photoautotrophy
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| GB/T 7714 | Ma, Ruijuan , Zhang, Zhen , Fang, Hong et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses [J]. | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) . |
| MLA | Ma, Ruijuan et al. "Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses" . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 16 . 1 (2023) . |
| APA | Ma, Ruijuan , Zhang, Zhen , Fang, Hong , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) . |
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Lutein is a high-value carotenoid with many human health benefits. Lycopene β- and ε-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of lycopene into distinct downstream branches, one of which is the lutein biosynthesis pathway, via α-carotene. Hence, LCYB and LCYE are key enzymes in lutein biosynthesis. In this study, the coding genes of two lycopene cyclases (CsLCYB and CsLCYE) of a lutein-enriched marine green microalga, Chlorella sorokiniana FZU60, were isolated and identified. A sequence analysis and computational modeling of CsLCYB and CsLCYE were performed using bioinformatics to identify the key structural domains. Further, a phylogenetic analysis revealed that CsLCYB and CsLCYE were homogeneous to the proteins of other green microalgae. Subcellular localization tests in Nicotiana benthamiana showed that CsLCYB and CsLCYE localized in chloroplasts. A pigment complementation assay in Escherichia coli revealed that CsLCYB could efficiently β-cyclize both ends of lycopene to produce β-carotene. On the other hand, CsLCYE possessed a strong ε-monocyclase activity for the production of δ-carotene and a weak ε-bicyclic activity for the production of ε-carotene. In addition, CsLCYE was able to catalyze lycopene into β-monocyclic γ-carotene and ultimately produced α-carotene with a β-ring and an ε-ring via γ-carotene or δ-carotene. Moreover, the co-expression of CsLCYB and CsLCYE in E. coli revealed that α-carotene was a major product, which might lead to the production of a high level of lutein in C. sorokiniana FZU60. The findings provide a theoretical foundation for performing metabolic engineering to improve lutein biosynthesis and accumulation in C. sorokiniana FZU60.
Keyword ：
lutein lutein lycopene cyclase lycopene cyclase α-carotene α-carotene β-carotene β-carotene
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| GB/T 7714 | Hong Fang , Junjie Liu , Ruijuan Ma et al. Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 [J]. | Marine Drugs , 2023 , 21 (7) . |
| MLA | Hong Fang et al. "Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60" . | Marine Drugs 21 . 7 (2023) . |
| APA | Hong Fang , Junjie Liu , Ruijuan Ma , Yiping Zou , Shih-Hsin Ho , Jianfeng Chen et al. Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 . | Marine Drugs , 2023 , 21 (7) . |
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Lutein is a high-value carotenoid with many human health benefits. Lycopene beta- and epsilon-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of lycopene into distinct downstream branches, one of which is the lutein biosynthesis pathway, via ff-carotene. Hence, LCYB and LCYE are key enzymes in lutein biosynthesis. In this study, the coding genes of two lycopene cyclases (CsLCYB and CsLCYE) of a lutein-enriched marine green microalga, Chlorella sorokiniana FZU60, were isolated and identified. A sequence analysis and computational modeling of CsLCYB and CsLCYE were performed using bioinformatics to identify the key structural domains. Further, a phylogenetic analysis revealed that CsLCYB and CsLCYE were homogeneous to the proteins of other green microalgae. Subcellular localization tests in Nicotiana benthamiana showed that CsLCYB and CsLCYE localized in chloroplasts. A pigment complementation assay in Escherichia coli revealed that CsLCYB could efficiently beta-cyclize both ends of lycopene to produce beta-carotene. On the other hand, CsLCYE possessed a strong epsilon-monocyclase activity for the production of delta-carotene and a weak "-bicyclic activity for the production of "-carotene. In addition, CsLCYE was able to catalyze lycopene into beta-monocyclic gamma-carotene and ultimately produced alpha-carotene with a beta-ring and an epsilon-ring via gamma-carotene or delta-carotene. Moreover, the co-expression of CsLCYB and CsLCYE in E. coli revealed that alpha-carotene was a major product, which might lead to the production of a high level of lutein in C. sorokiniana FZU60. The findings provide a theoretical foundation for performing metabolic engineering to improve lutein biosynthesis and accumulation in C. sorokiniana FZU60.
Keyword ：
alpha-carotene alpha-carotene beta-carotene beta-carotene Chlorella sorokiniana FZU60 Chlorella sorokiniana FZU60 lutein lutein lycopene cyclase lycopene cyclase
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| GB/T 7714 | Fang, Hong , Liu, Junjie , Ma, Ruijuan et al. Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 [J]. | MARINE DRUGS , 2023 , 21 (7) . |
| MLA | Fang, Hong et al. "Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60" . | MARINE DRUGS 21 . 7 (2023) . |
| APA | Fang, Hong , Liu, Junjie , Ma, Ruijuan , Zou, Yiping , Ho, Shih-Hsin , Chen, Jianfeng et al. Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 . | MARINE DRUGS , 2023 , 21 (7) . |
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