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响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 PKU
期刊论文 | 2024 , 1-10 | 食品工业科技
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Abstract :

为实现鱿鱼加工下脚料鱿鱼皮的高值化利用,本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数(Trichloroacetic acid-nitrogen soluble index,TCA-NSI)为评价指标,采用单因素和响应面试验优化酶解工艺参数,并对所制备蛋白肽的氨基酸组成、分子量分布、蛋白肽序列、抗氧化活性及其体外模拟消化特性进行表征。结果表明,最佳酶解工艺条件为酶解温度43.50 ℃、酶解时间100 min、酶解pH7.50、胰蛋白酶与碱性蛋白酶比例2:1、加酶量4000 U/g,在此条件下其TCA-NSI可达89.02%±0.66%。所制备的鱿鱼皮蛋白肽其氨基酸组成均衡,必需氨基酸指数(Essential amino acid index,EAAI)可达0.90。此外,鱿鱼皮蛋白肽含有43个多肽序列,其中38个肽段的分子质量低于1800 Da,其DPPH自由基、ABTS+自由基和羟自由基清除率的IC50分别为0.61、0.28和1.95 mg/mL,且经体外模拟胃肠消化后仍能维持较好的抗氧化活性。本研究结果为鱿鱼加工下脚料的开发利用和精深加工提供了理论依据。

Keyword :

体外模拟消化 体外模拟消化 抗氧化活性 抗氧化活性 理化性质 理化性质 蛋白肽 蛋白肽 鱿鱼皮 鱿鱼皮

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GB/T 7714 朱清清 , 马瑞娟 , 陈剑锋 et al. 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 [J]. | 食品工业科技 , 2024 : 1-10 .
MLA 朱清清 et al. "响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析" . | 食品工业科技 (2024) : 1-10 .
APA 朱清清 , 马瑞娟 , 陈剑锋 , 谢友坪 . 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 . | 食品工业科技 , 2024 , 1-10 .
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Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties EI PKU
期刊论文 | 2024 , 45 (7) , 217-226 | Science and Technology of Food Industry
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To achieve the high-value utilization of squid skin obtained from squid processing, this study utilized an enzymatic approach to prepare protein peptides. The enzymatic hydrolysis parameters were optimized using single factor and response surface tests, with the concentration of peptide and trichloroacetic acid-nitrogen soluble index (TCA-NSI) serving as evaluation criteria. The amino acid composition, molecular weight distribution, peptide sequence, antioxidant activity, and in vitro simulated digestive properties of the prepared protein peptides were also thoroughly characterized. The results showed that the optimal conditions for enzymatic hydrolysis were as follows: Enzymatic temperature of 43.50 ℃, enzymatic time of 100 minutes, enzymatic pH of 7.50, trypsin to alkaline protease ratio of 2:1, and enzyme dosage of 4000 U/g. Under these specified conditions, the TCA-NSI achieved a value of 89.02%±0.66%. The prepared protein peptides exhibited a well-balanced amino acid composition, with an essential amino acid index (EAAI) value of 0.90. Furthermore, the prepared protein peptides encompassed a total of 43 polypeptide sequences, with 38 of these segments exhibiting molecular weights below 1800 Da. The IC50 values for scavenging DPPH radical, ABTS+ radical, and hydroxyl radical were determined to be 0.61, 0.28 and 1.95 mg/mL, respectively. Notably, the antioxidant activity remained favorable even after undergoing simulated gastrointestinal digestion in vitro. These findings serve to establish a solid theoretical basis for the advancement and exploitation of byproducts derived from squid processing, as well as their subsequent value-added utilization. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.

Keyword :

Alkalinity Alkalinity Amino acids Amino acids Antioxidants Antioxidants Enzymatic hydrolysis Enzymatic hydrolysis Molecular weight distribution Molecular weight distribution Peptides Peptides Physicochemical properties Physicochemical properties Shellfish Shellfish Trichloroacetic acid Trichloroacetic acid

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GB/T 7714 Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen et al. Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties [J]. | Science and Technology of Food Industry , 2024 , 45 (7) : 217-226 .
MLA Qingqing, Zhu et al. "Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties" . | Science and Technology of Food Industry 45 . 7 (2024) : 217-226 .
APA Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen , Youping, Xie . Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties . | Science and Technology of Food Industry , 2024 , 45 (7) , 217-226 .
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基于快照式多光谱特征波长的小球藻叶黄素产量快速测定
期刊论文 | 2024 , 44 (8) , 2216-2223 | 光谱学与光谱分析
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叶黄素是天然的抗氧化剂,对人体健康有多种益处,异养小球藻具有叶黄素纯度和产量均较高的优势,而小球藻叶黄素产量主要取决于生物质产量和叶黄素含量两个因素.传统的光密度法测生物质产量和高效液相色谱法测叶黄素含量存在操作复杂、时效性低等不足.为了快速、无损测定小球藻生长过程中叶黄素含量变化,搭建可见-近红外双模式快照式多光谱成像检测系统,根据光谱响应区域,分别利用可见光相机获取叶黄素光谱信息,近红外相机获取生物质光谱信息,构建含有生物质量和叶黄素含量信息的可见-近红外双模式多光谱数据集.针对系统所使用的快照式多光谱相机光谱范围宽、波长数量少的特征波长选取问题,提出一种结合序列浮动前向选择的改进型连续投影算法(mSPA);将mSPA与常规的连续投影算法、遗传算法及随机蛙跳三种波长选择算法作对比分析后,构建了基于特征波长的多元线性回归和极限学习机模型;最后,利用生物质产量和叶黄素含量的最佳预测模型生成小球藻叶黄素产量的可视化分布图.结果表明,在利用近红外、可见光相机分别检测小球藻生物质、叶黄素量时,mSPA得到的特征波长数均较少,并具有最高的预测精度.生物质量与叶黄素含量的最佳模型均为mSPA筛选特征波长后建立的极限学习机模型,对应的预测集决定系数分别为0.947和0.907,预测集均方根误差分别为0.698 g·L-1和0.077 mg·g-1,剩余预测偏差分别为3.535和3.338,模型的预测能力较好.可视化分布实现了直观监测小球藻叶黄素产量的变化,有助于后续实际生产中在线检测叶黄素产量.mSPA在快照式多光谱检测小球藻生物质含量及叶黄素含量中,通过对排序波长逐个评估以选择出最佳特征波长组合,有效地避免了特征波长的错选、漏选,提高了模型的预测精度,为快照式多光谱成像技术应用提供新的波长选择思路.

Keyword :

叶黄素产量 叶黄素产量 小球藻 小球藻 快照式多光谱 快照式多光谱 特征波长 特征波长

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GB/T 7714 沈英 , 占秀兴 , 黄春红 et al. 基于快照式多光谱特征波长的小球藻叶黄素产量快速测定 [J]. | 光谱学与光谱分析 , 2024 , 44 (8) : 2216-2223 .
MLA 沈英 et al. "基于快照式多光谱特征波长的小球藻叶黄素产量快速测定" . | 光谱学与光谱分析 44 . 8 (2024) : 2216-2223 .
APA 沈英 , 占秀兴 , 黄春红 , 谢友坪 , 郭翠霞 , 黄峰 . 基于快照式多光谱特征波长的小球藻叶黄素产量快速测定 . | 光谱学与光谱分析 , 2024 , 44 (8) , 2216-2223 .
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Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) SCIE
期刊论文 | 2024 , 80 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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Chlorella meal is considered a promising alternative to fish meal in aquafeeds, but the effects of incorporating Chlorella meal as a feed ingredient on the growth and quality of eel have not been explored. The effects of partially substituting fish meal (0 %, 5 %, 10 %, 15 %, and 20 %) with Chlorella meal on the growth performance, whole-body composition, pigmentation, and physiological health of marbled eel (Anguilla marmorata) were evaluated in this study. The results revealed that dietary inclusion of Chlorella meal significantly increased the final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR), but it significantly decreased the feed conversion rate (FCR), with these parameters exhibiting linear trends (P < 0.05). The whole-body protein and fat contents were significantly higher in all groups fed with diets containing Chlorella meal compared to the control group (P < 0.05). In addition, the dorsal and abdominal skin of marbled eel exhibited an orange-yellow color, and the color intensity increased with increasing levels of dietary Chlorella meal. The color change corresponded to increased values of a* (redness), b* (yellowness), and lutein content in the fish skin (P < 0.05). The physiological health of marbled eel also improved in the groups where Chlorella meal was included. The optimal replacement rate was approximately 10 % (9 % Chlorella meal supplementation), because the whole-body protein content and most physiological health parameters were highest under this condition, and the growth performance did not show a significant increase when the fish meal replacement rate exceeded 10 % (P > 0.05). These findings indicate the potential of Chlorella meal as a promising alternative to fish meal in feed formulations, offering benefits in enhancing growth performance and quality of marbled eel.

Keyword :

Chlorella meal Chlorella meal Growth performance Growth performance Marbled eel Marbled eel Physiological health Physiological health Pigmentation Pigmentation Whole-body composition Whole-body composition

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GB/T 7714 Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 .
MLA Huang, Kaiyun et al. "Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata )" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 80 (2024) .
APA Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 .
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Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses SCIE
期刊论文 | 2023 , 16 (1) | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
WoS CC Cited Count: 3
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BackgroundChlorella sorokiniana FZU60 is a promising lutein producing microalga. A mixotrophy/photoautotrophy two-stage strategy can achieve high biomass concentration at stage 1 and high lutein content at stage 2, leading to excellent lutein production efficiency in C. sorokiniana FZU60. However, the underlying molecular mechanisms are still unclear, restraining the further improvement of lutein production.ResultsIn this study, physiological and biochemical analysis revealed that photochemical parameters (Fv/Fm and NPQ) and photosynthetic pigments contents increased during the shift from mixotrophy to photoautotrophy, indicating that photosynthesis and photoprotection enhanced. Furthermore, transcriptomic analysis revealed that the glyoxylate cycle and TCA cycle were suppressed after the shift to photoautotrophy, leading to a decreased cell growth rate. However, the gene expression levels of photosynthesis, CO2 fixation, autophagy, and lutein biosynthesis were upregulated at the photoautotrophy stage, demonstrating that microalgal cells could obtain more precursor to synthesize lutein for enhancing photosynthesis and reducing reactive oxygen species.ConclusionsThe findings help to elucidate the molecular mechanisms for high lutein production efficiency of C. sorokiniana FZU60 under the mixotrophy/photoautotrophy strategy, identify key functional genes responsible for lutein biosynthesis, and shed light on further improvement of lutein production by genetic or metabolic engineering in future studies.

Keyword :

Chlorella sorokiniana FZU60 Chlorella sorokiniana FZU60 Lutein Lutein Mixotrophy Mixotrophy Molecular mechanisms Molecular mechanisms Photoautotrophy Photoautotrophy

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GB/T 7714 Ma, Ruijuan , Zhang, Zhen , Fang, Hong et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses [J]. | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) .
MLA Ma, Ruijuan et al. "Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses" . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 16 . 1 (2023) .
APA Ma, Ruijuan , Zhang, Zhen , Fang, Hong , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) .
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Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 SCIE
期刊论文 | 2023 , 71 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
WoS CC Cited Count: 2
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Microalgae can accumulate various compounds simultaneously, thus co-production of high-value metabolites to improve the economic value of microalgae production is feasible. In this study, a microalga strain Nitzschia sp. FZU62 capable of co-producing fucoxanthin and eicosapentaenoic acid (EPA) was isolated and identified. Further, heterotrophic conditions were optimized to improve the co-production efficiency in flasks. Results showed that the optimal conditions for flask culture were cultivation for 84 h with an inoculum size of 0.47 g/L and a temperature of 19 degrees C. Besides, three fed-batch culture strategies with distinct nutrient compositions were explored to improve the heterotrophic performance of strain FZU62. Results indicated that the highest biomass, fucoxanthin, and EPA productivities were obtained by Fed-batch III, in which concentrated medium was pulse -fed to supply 60 % initial nutrients once glucose concentration dropped to about 2 g/L. Moreover, the hetero-trophic performance was verified in a 5 L fermenter, resulting in excellent fucoxanthin and EPA productions of 63.03 and 398.60 mg/L, and productivities of 3.41 and 22.52 mg/L/d, respectively. These results, especially the EPA production and productivity, are superior to many literature reports. The present study provides new in-sights into the highly efficient co-production of fucoxanthin and EPA by marine microalgae and has guiding significance for commercial production in heterotrophic mode.

Keyword :

Co -production Co -production Eicosapentaenoic acid Eicosapentaenoic acid Fucoxanthin Fucoxanthin Heterotrophic cultivation Heterotrophic cultivation Microalgae Microalgae

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GB/T 7714 Ma, Ruijuan , You, Yuanwei , Liu, Xinyu et al. Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 .
MLA Ma, Ruijuan et al. "Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 71 (2023) .
APA Ma, Ruijuan , You, Yuanwei , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping , Chen, Jianfeng . Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 .
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Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 SCIE
期刊论文 | 2023 , 21 (7) | MARINE DRUGS
WoS CC Cited Count: 1
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Lutein is a high-value carotenoid with many human health benefits. Lycopene beta- and epsilon-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of lycopene into distinct downstream branches, one of which is the lutein biosynthesis pathway, via ff-carotene. Hence, LCYB and LCYE are key enzymes in lutein biosynthesis. In this study, the coding genes of two lycopene cyclases (CsLCYB and CsLCYE) of a lutein-enriched marine green microalga, Chlorella sorokiniana FZU60, were isolated and identified. A sequence analysis and computational modeling of CsLCYB and CsLCYE were performed using bioinformatics to identify the key structural domains. Further, a phylogenetic analysis revealed that CsLCYB and CsLCYE were homogeneous to the proteins of other green microalgae. Subcellular localization tests in Nicotiana benthamiana showed that CsLCYB and CsLCYE localized in chloroplasts. A pigment complementation assay in Escherichia coli revealed that CsLCYB could efficiently beta-cyclize both ends of lycopene to produce beta-carotene. On the other hand, CsLCYE possessed a strong epsilon-monocyclase activity for the production of delta-carotene and a weak "-bicyclic activity for the production of "-carotene. In addition, CsLCYE was able to catalyze lycopene into beta-monocyclic gamma-carotene and ultimately produced alpha-carotene with a beta-ring and an epsilon-ring via gamma-carotene or delta-carotene. Moreover, the co-expression of CsLCYB and CsLCYE in E. coli revealed that alpha-carotene was a major product, which might lead to the production of a high level of lutein in C. sorokiniana FZU60. The findings provide a theoretical foundation for performing metabolic engineering to improve lutein biosynthesis and accumulation in C. sorokiniana FZU60.

Keyword :

alpha-carotene alpha-carotene beta-carotene beta-carotene Chlorella sorokiniana FZU60 Chlorella sorokiniana FZU60 lutein lutein lycopene cyclase lycopene cyclase

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GB/T 7714 Fang, Hong , Liu, Junjie , Ma, Ruijuan et al. Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 [J]. | MARINE DRUGS , 2023 , 21 (7) .
MLA Fang, Hong et al. "Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60" . | MARINE DRUGS 21 . 7 (2023) .
APA Fang, Hong , Liu, Junjie , Ma, Ruijuan , Zou, Yiping , Ho, Shih-Hsin , Chen, Jianfeng et al. Functional Characterization of Lycopene beta- and epsilon-Cyclases from a Lutein-Enriched Green Microalga Chlorella sorokiniana FZU60 . | MARINE DRUGS , 2023 , 21 (7) .
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基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究 PKU
期刊论文 | 2023 , 1-9 | 食品工业科技
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为构建索罗金小球藻(Chlorella sorokiniana FZU60)的异养无菌体系,对其共生菌进行分离和鉴定,并考察了8种常见抗生素(头孢噻肟、卡那霉素、新霉素、氨苄青霉素、链霉素、四环素、氯霉素和红霉素)对共生菌和藻株FZU60生长的影响。结果表明:异养体系中主要的共生菌为芽孢杆菌(Bacillus)(78.76%)和甲基杆菌(Methylobacterium)(3.14%),且10 mg/L头孢噻肟、10 mg/L四环素、100 mg/L卡那霉素和100 mg/L红霉素对这两株共生菌均具有较好抑制效果,但对藻株FZU60的生长无明显影响。在此基础上,进一步考察了不同抗生素组合对藻株FZU60异养共生菌的抑制效果,发现采用10 mg/L头孢噻肟、10 mg/L四环素和100 mg/L卡那霉素的抗生素组合可成功构建异养无菌体系,且经3次传代培养后藻株FZU60仍可维持稳定的生长及无菌状态。本实验结果可为索罗金小球藻高密度异养中无菌藻种的制备提供一定的理论参考和实验依据。

Keyword :

异养培养 异养培养 抗生素 抗生素 无菌培养 无菌培养 索罗金小球藻 索罗金小球藻

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GB/T 7714 蔡伟鑫 , 刘心宇 , 马瑞娟 et al. 基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究 [J]. | 食品工业科技 , 2023 : 1-9 .
MLA 蔡伟鑫 et al. "基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究" . | 食品工业科技 (2023) : 1-9 .
APA 蔡伟鑫 , 刘心宇 , 马瑞娟 , 陈剑锋 , 谢友坪 . 基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究 . | 食品工业科技 , 2023 , 1-9 .
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Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity; [基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究] Scopus PKU
期刊论文 | 2023 , 44 (12) , 148-156 | Science and Technology of Food Industry
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To construct a heterotrophic axenic system of Chlorella sorokiniana FZU60, the symbiotic bacteria were isolated and identified, and the effects of eight common antibiotics (cefotaxime, kanamycin, neomycin, ampicillin, streptomycin, tetracycline, chloramphenicol, and erythromycin) on the cell growth of symbiotic bacteria and C. sorokiniana FZU60 were investigated. Results showed that the main symbiotic bacteria were Bacillus (78.76%) and Methylobacterium (3.14%). Besides, 10 mg/L cefotaxime, 10 mg/L tetracycline, 100 mg/L kanamycin, and 100 mg/L erythromycin showed excellent inhibitory effect on these two symbiotic bacteria but no obvious effect on the cell growth of C. sorokiniana FZU60. Based on the above-mentioned results, the inhibition effect of different antibiotic combinations on symbiotic bacteria of C. sorokiniana FZU60 was further investigated. It was found that the heterotrophic axenic system could be successfully constructed by using the combination of 10 mg/L cefotaxime, 10 mg/L tetracycline, and 100 mg/L kanamycin. Moreover, C. sorokiniana FZU60 could maintain stable and axenic growth after three generations. The experimental results would provide theoretical reference and experimental basis for the preparation of axenic microalgal seed for high-cell-density heterotrophic cultivation of C. sorokiniana. © 2023 Science and Technology of Food Industry. All rights reserved.

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antibiotic antibiotic axenic cultivation axenic cultivation Chlorella sorokiniana Chlorella sorokiniana heterotrophic cultivation heterotrophic cultivation

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GB/T 7714 Cai, W. , Liu, X. , Ma, R. et al. Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity; [基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究] [J]. | Science and Technology of Food Industry , 2023 , 44 (12) : 148-156 .
MLA Cai, W. et al. "Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity; [基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究]" . | Science and Technology of Food Industry 44 . 12 (2023) : 148-156 .
APA Cai, W. , Liu, X. , Ma, R. , Chen, J. , Xie, Y. . Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity; [基于抗生素敏感性构建索罗金小球藻异养无菌体系的研究] . | Science and Technology of Food Industry , 2023 , 44 (12) , 148-156 .
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可见/近红外快照式多光谱成像快速测定雨生红球藻虾青素含量 PKU
期刊论文 | 2023 , 1-8 | 食品工业科技
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Abstract :

为了实现快速、无损检测雨生红球藻虾青素含量,本文提出一种快照式多光谱成像检测方法。利用可见光光谱范围480~635 nm和近红光谱范围665~950 nm的2台快照式多光谱相机搭建成像系统,采集了不同生长周期下的雨生红球藻样品光谱数据。为了优化预测模型,对比了不同处理方法的组合,包括不同光谱范围、3种预处理方法、2种特征波长选择算法和2种建模方法。结果表明,可见与近红外联用光谱经一阶导数(first derivation,FD)预处理、竞争自适应重加权采样(competitive adaptive reweighted sampling,CARS)选择特征波长和反向传播(back propagation,BP)神经网络建模所构建的模型预测效果最佳,预测集相关系数(Rp)为0.9622,预测集均方根误差(root mean square error of prediction,RMSEP)为0.5126,剩余预测偏差(residual predictive deviation,RPD)为3.6726,优于仅用可见光光谱(Rp为0.9467, RMSEP为0.6065,RPD为3.1042)。说明快照式多光谱成像技术检测雨生红球藻虾青素含量是可行的,并且可见与近红外光谱联用效果更好。

Keyword :

可见/近红外 可见/近红外 多光谱成像 多光谱成像 快照式 快照式 快速检测 快速检测 虾青素含量 虾青素含量

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GB/T 7714 沈英 , 占秀兴 , 黄春红 et al. 可见/近红外快照式多光谱成像快速测定雨生红球藻虾青素含量 [J]. | 食品工业科技 , 2023 : 1-8 .
MLA 沈英 et al. "可见/近红外快照式多光谱成像快速测定雨生红球藻虾青素含量" . | 食品工业科技 (2023) : 1-8 .
APA 沈英 , 占秀兴 , 黄春红 , 谢友坪 , 黄峰 . 可见/近红外快照式多光谱成像快速测定雨生红球藻虾青素含量 . | 食品工业科技 , 2023 , 1-8 .
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