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学者姓名：何小镇
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Abstract ：
Background The outer membrane vesicles (OMVs) derived from Porphyromonas gingivalis (P. gingivalis) have long been acknowledged for their crucial role in the initiation of periodontitis. However, the implications of P. gingivalis OMVs in the context of cardiovascular disease (CVD) remain incompletely understood. This study aimed to clarify both the impact and the underlying mechanisms through which P. gingivalis OMVs contribute to the propagation of distal cardiovascular inflammation and trauma.Methods In this study, various concentrations (0, 1.25, 2.5, and 4.5 mu g/mu L) of P. gingivalis OMVs were microinjected into the common cardinal vein of zebrafish larvae at 48 h post-fertilization (hpf) to assess changes in cardiovascular injury and inflammatory response. Zebrafish larvae from both the PBS and the 2.5 mu g/mu L injection cohorts were harvested at 30 h post-injection (hpi) for transcriptional analysis. Real-time quantitative PCR (RT-qPCR) was employed to evaluate relative gene expression.Results These findings demonstrated that P. gingivalis OMVs induced pericardial enlargement in zebrafish larvae, caused vascular damage, increased neutrophil counts, and activated inflammatory pathways. Transcriptomic analysis further revealed the involvement of the immune response and the extracellular matrix (ECM)-receptor interaction signaling pathway in this process.Conclusion This study illuminated potential mechanisms through which P. gingivalis OMVs contribute to CVD. It accentuated their involvement in distal cardiovascular inflammation and emphasizes the need for further research to comprehensively grasp the connection between periodontitis and CVD.
Keyword ：
Cardiovascular disease Cardiovascular disease Outer membrane vesicles Outer membrane vesicles Periodontitis Periodontitis Porphyromonas gingivalis Porphyromonas gingivalis Transcriptome Transcriptome
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| GB/T 7714 | Guo, Jianbin , Lin, Kaijin , Wang, Siyi et al. Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury [J]. | BMC ORAL HEALTH , 2024 , 24 (1) . |
| MLA | Guo, Jianbin et al. "Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury" . | BMC ORAL HEALTH 24 . 1 (2024) . |
| APA | Guo, Jianbin , Lin, Kaijin , Wang, Siyi , He, Xiaozhen , Huang, Zhen , Zheng, Minqian . Effects and mechanisms of Porphyromonas gingivalis outer membrane vesicles induced cardiovascular injury . | BMC ORAL HEALTH , 2024 , 24 (1) . |
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As a vertebrate model, zebrafish (Danio rerio) plays a vital role in the field of life sciences. Recently, gene-editing technology has become increasingly innovative, significantly promoting scientific research on zebrafish. However, the implementation of these methods in a reasonable and accurate manner to achieve efficient gene-editing remains challenging. In this review, we systematically summarize the development and latest progress in zebrafish gene-editing technology. Specifically, we outline trends in double-strand break-free genome modification and the prospective applications of fixed-point orientation transformation of any base at any location through a multi-method approach.
Keyword ：
Danio rerio Danio rerio double-stranded break double-stranded break fixed-point orientation transformation fixed-point orientation transformation gene editing gene editing genome modification genome modification nick nick vertebrate model vertebrate model zebrafish zebrafish
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| GB/T 7714 | Li, Yanling , Jia, Zhipeng , Zhang, Shuchao et al. Progress in Gene-Editing Technology of Zebrafish [J]. | BIOMOLECULES , 2021 , 11 (9) . |
| MLA | Li, Yanling et al. "Progress in Gene-Editing Technology of Zebrafish" . | BIOMOLECULES 11 . 9 (2021) . |
| APA | Li, Yanling , Jia, Zhipeng , Zhang, Shuchao , He, Xiaozhen . Progress in Gene-Editing Technology of Zebrafish . | BIOMOLECULES , 2021 , 11 (9) . |
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Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.
Keyword ：
Acrylamide Acrylamide curcumin curcumin developmental toxicity developmental toxicity (-)- epigallocatechin gallate (-)- epigallocatechin gallate genotoxicity genotoxicity human lymphocytes human lymphocytes zebrafish embryos zebrafish embryos
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| GB/T 7714 | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity [J]. | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) : 199-218 . |
| MLA | Wang, Shanshan et al. "Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity" . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY 103 . 2 (2021) : 199-218 . |
| APA | Wang, Shanshan , Wang, Hufei , Chen, Yanqiu , Liu, Jilai , He, Xiaozhen , Huang, Da et al. Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity . | TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY , 2021 , 103 (2) , 199-218 . |
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提供了一种在鱼胚胎中精确剪切位点特异性RNA的技术， 采用CaSrx介导的RNA编辑技术，只需提供CaSrx mRNA和特异性靶向引导RNA，即可实现对鱼胚胎中位点特异性RNA的精确敲除，具有高效，特异性强，方便，成本低等诸多优点。 本发明克服了现有siRNA技术效率低，吗啉代介导的基因敲除技术成本高，效果不理想的问题。
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| GB/T 7714 | He Xiaozhen , Zhong Lili . 一种精确剪切鱼胚胎中位点特异性RNA的技术 : ZA202110568[P]. | 2021-12-17 . |
| MLA | He Xiaozhen et al. "一种精确剪切鱼胚胎中位点特异性RNA的技术" : ZA202110568. | 2021-12-17 . |
| APA | He Xiaozhen , Zhong Lili . 一种精确剪切鱼胚胎中位点特异性RNA的技术 : ZA202110568. | 2021-12-17 . |
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Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
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| GB/T 7714 | He, Xiaozhen , Chen, Wenfeng , Liu, Zhen et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species [J]. | NUCLEIC ACIDS RESEARCH , 2020 , 48 (10) . |
| MLA | He, Xiaozhen et al. "Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species" . | NUCLEIC ACIDS RESEARCH 48 . 10 (2020) . |
| APA | He, Xiaozhen , Chen, Wenfeng , Liu, Zhen , Yu, Guirong , Chen, Youbang , Cai, Yi-Jun et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species . | NUCLEIC ACIDS RESEARCH , 2020 , 48 (10) . |
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