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学者姓名：王国增
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Abstract ：
为了探究微塑料生物毒性研究现状、热点及趋势，以Web of Science核心合集和中国知网（www.cnki.net）为数据源，基于文献计量可视化软件VOS viewer对2011年以来与微塑料生物毒性相关的文献进行文章产生趋势分析、研究作者分析、期刊和引文分析和关键词聚类分析。结果表明，关于微塑料生物毒性的文章年发表数量呈指数增长；全球范围内中国学者发表文章总数和被引量均位居世界前列；《Science of the Total Environment》《Journal of Hazardous Materials》《Environmental Pollution》《Environmental Science&Technology》等期刊在微塑料生物毒性研究中具有较高的影响力；微塑料生物毒性的研究热点主要集中在“微塑料生物毒性的表现”“微塑料生物毒性的来源”“微塑料与其他有害物质的联合生物毒性”和“微塑料的摄入途径”这四个方面。未来微塑料生物毒性研究需要将建立微塑料的快速检测技术、明确不同暴露途径下微塑料吸收和转移机制及探索内源性微塑料的防治措施作为重点研发领域。
Keyword ：
VOS viewer VOS viewer 可视化分析 可视化分析 微塑料 微塑料 生物毒性 生物毒性 研究热点 研究热点
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| GB/T 7714 | 肖庆 , 王国增 , 韩静 et al. 微塑料生物毒性研究现状及热点可视化剖析 [J]. | 生物技术进展 , 2023 , 13 (04) : 619-627 . |
| MLA | 肖庆 et al. "微塑料生物毒性研究现状及热点可视化剖析" . | 生物技术进展 13 . 04 (2023) : 619-627 . |
| APA | 肖庆 , 王国增 , 韩静 , 刘小兰 . 微塑料生物毒性研究现状及热点可视化剖析 . | 生物技术进展 , 2023 , 13 (04) , 619-627 . |
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采用高通量扩增子测序技术,对山羊瘤胃微生物宏基因组第10 家族(GH10)和第11 家族(GH11)木聚糖酶基因多样性进行分析.经序列拼接、过滤和可操作分类单元聚类(<95％),获得 348 个GH10 木聚糖酶基因.GH10 木聚糖酶基因片段与GenBank数据库中已知蛋白序列的一致性为 46％～97％,其中一半以上的序列与已知木聚糖酶的一致性低于 80％.序列注释结果表明,GH10 木聚糖酶基因分布于 5 个门,其中拟杆菌门(Bacte-roidetes)和厚壁菌门(Firmicutes)为优势门.经序列处理分析,获得 143 个GH11 木聚糖酶基因,与GenBank中已知蛋白序列的一致性为 51％～100％.GH11 木聚糖酶基因分布于 6 个门,其中子囊菌门(Ascomycota)和放线菌门(Actinobacteria)为优势门.基于片段序列和染色体步移技术,获得两个新的全长木聚糖酶基因,由其编码的蛋白具有特殊的结构域组成.本研究表明,山羊瘤胃环境中GH10 木聚糖酶基因的多样性远高于GH11,且两者的分布存在较大差异.此外,该环境中还存在大量的新木聚糖酶基因,可为后续木聚糖酶基因资源的发掘和应用奠定基础.
Keyword ：
基因多样性 基因多样性 宏基因组学 宏基因组学 扩增子测序 扩增子测序 木聚糖酶 木聚糖酶 瘤胃 瘤胃
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| GB/T 7714 | 张心怡 , 谢秀烨 , 苏华清 et al. 基于扩增子测序的瘤胃内含物中木聚糖酶基因多样性研究 [J]. | 福州大学学报（自然科学版） , 2023 , 51 (3) : 431-438 . |
| MLA | 张心怡 et al. "基于扩增子测序的瘤胃内含物中木聚糖酶基因多样性研究" . | 福州大学学报（自然科学版） 51 . 3 (2023) : 431-438 . |
| APA | 张心怡 , 谢秀烨 , 苏华清 , 石贤爱 , 王国增 . 基于扩增子测序的瘤胃内含物中木聚糖酶基因多样性研究 . | 福州大学学报（自然科学版） , 2023 , 51 (3) , 431-438 . |
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Pseudostellaria heterophylla, also called Tai-zi-shen (TZS) in Traditional Chinese Medicine (TCM), is always used clinically to treat spleen deficiency symptoms. Polysaccharides in TZS have various pharmacological activities, including anti-diabetic, immune regulation, and myocardial protection. However, the relationship between the spleen-invigorating effects of TZS or its polysaccharides and intestinal flora are not clear. This study investigated the effects of TZS decoction (PHD) and polysaccharide (PHP) on immune function and intestinal flora in a rat model of spleen deficiency syndrome (SDS) induced by a decoction of raw rhubarb (RRD). PHD and PHP increased immune organ index, alleviated inflammatory cell filtration, and reduced the levels of pro-inflammatory cytokines in rats with spleen deficiency syndrome. In addition, the production of butyric acid was promoted in PHD and PHP groups. Moreover, 16S rRNA gene sequencing showed that PHD and PHP reduced the relative abundance of Firmicutes while increasing the one of Bacteroidetes; significantly increased the abundance of Lactobacillus and decreased the abundance of Rombutsia; and PHP significantly increased the abundance of Alloprevotella. And there was a significant positive correlation between the alleviation of SDS and short-chain fatty acids (SCFAs)-producing bacteria. These findings suggested PHD and PHP, especially PHP, has a potential to relieve spleen deficiency by reducing intestinal inflammation, modulating structure and composition of gut microbiota, and promoting the production of butyric acid.
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| GB/T 7714 | Xiao, Qing , Zhao, Li , Jiang, Chang et al. Polysaccharides from Pseudostellaria heterophylla modulate gut microbiota and alleviate syndrome of spleen deficiency in rats [J]. | SCIENTIFIC REPORTS , 2022 , 12 (1) . |
| MLA | Xiao, Qing et al. "Polysaccharides from Pseudostellaria heterophylla modulate gut microbiota and alleviate syndrome of spleen deficiency in rats" . | SCIENTIFIC REPORTS 12 . 1 (2022) . |
| APA | Xiao, Qing , Zhao, Li , Jiang, Chang , Zhu, Yanjin , Zhang, Jizhou , Hu, Juan et al. Polysaccharides from Pseudostellaria heterophylla modulate gut microbiota and alleviate syndrome of spleen deficiency in rats . | SCIENTIFIC REPORTS , 2022 , 12 (1) . |
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Omega-transaminases (omega-TAs) have attracted considerable interest for their use in asymmetric synthesis of chiral amines with a high degree of optical purity and yield. The rapid evaluation of the characteristics of newly identified or engineered omega-TAs is important for industrial applications. In this study, a visible spectrophotometric assay was developed for rapid quantitative determination of omega-TA activities based on the transamination of 2-(4-nitrophenyl)ethan-1-amine to generate a red product (E)-N-(4-nitrophenethyl)-2-(4-nitrophenyl)ethen-1-amine. After various co-solvents were evaluated, dimethyl sulfoxide (DMSO) was considered optimal because the red product exhibits good solubility and retains its original color. The red product dissolved in DMSO has its highest absorbance at 465 nm, and its concentration has a good linear relationship with the absorbance. A spectro-photometric assay was established and validated using conventional HPLC analysis (<10% divergence). This method was then used to characterize an omega-TA from thermophilic Geobacillus thermoleovorans and an omega-TA ob-tained from the metagenome of a soda lake. The results demonstrated that omega-transaminase enzymatic properties could be characterized simply, rapidly, and at low cost, using this newly established visible spectrophotometric assay method.
Keyword ：
Enzyme characterization Enzyme characterization Spectrophotometric assay Spectrophotometric assay ?-Transaminase ?-Transaminase UV UV Vis Vis
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| GB/T 7714 | Wang, Guozeng , Jiang, Zhihao , Xiao, Qing et al. Visible spectrophotometric assay for characterization of omega-transaminases [J]. | ANALYTICAL BIOCHEMISTRY , 2022 , 658 . |
| MLA | Wang, Guozeng et al. "Visible spectrophotometric assay for characterization of omega-transaminases" . | ANALYTICAL BIOCHEMISTRY 658 (2022) . |
| APA | Wang, Guozeng , Jiang, Zhihao , Xiao, Qing , Jiang, Chang , Shi, Xian'ai . Visible spectrophotometric assay for characterization of omega-transaminases . | ANALYTICAL BIOCHEMISTRY , 2022 , 658 . |
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目的：从高温温泉宏基因组中挖掘能够高效催化合成稀有糖的新耐高温D-来苏糖异构酶。方法：从云南昌宁鸡飞温泉底泥中提取宏基因组DNA并进行高通量测序，经基因注释及序列比对鉴定D-来苏糖异构酶基因，构建大肠杆菌异源表达载体并诱导表达，通过亲和层析纯化重组蛋白并对其性质研究。结果：从温泉底泥宏基因组测序结果中鉴定得到8个D-来苏糖异构酶基因，选择4个基因进行异源表达，其中JF-LI1和JF-LI4在大肠杆菌中成功表达并检测到酶活性。研究表明，JF-LI1和JF-LI4的最适温度分别为70℃和75℃。JF-LI4具有较宽的作用温度和良好的热稳定性，在30～100℃的温度范围内剩余40%以上的酶活力。JF...
Keyword ：
D-来苏糖异构酶 D-来苏糖异构酶 功能糖 功能糖 宏基因组 宏基因组 稀有糖 稀有糖 高温温泉 高温温泉
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| GB/T 7714 | 苏雅丽 , 陈凤贞 , 石贤爱 et al. 温泉宏基因组来源的D-来苏糖异构酶性质研究 [J]. | 中国生物工程杂志 , 2022 , 42 (09) : 27-38 . |
| MLA | 苏雅丽 et al. "温泉宏基因组来源的D-来苏糖异构酶性质研究" . | 中国生物工程杂志 42 . 09 (2022) : 27-38 . |
| APA | 苏雅丽 , 陈凤贞 , 石贤爱 , 王国增 . 温泉宏基因组来源的D-来苏糖异构酶性质研究 . | 中国生物工程杂志 , 2022 , 42 (09) , 27-38 . |
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碱性蛋白酶作为广泛应用的蛋白酶之一,具有重要的工业应用价值。发掘优质的碱性蛋白酶基因,实现其高效表达,有助于更好的满足工业应用需求。分别将Cordyceps fumosorosea和Beauveria bassiana来源的碱性蛋白酶基因pa1及pa2与表达载体pPIC9连接,并在毕赤酵母GS115中实现高效表达。对于纯化后的重组蛋白PA1及PA2的酶学性质进行测定,二者最适pH均为8.5,最适温度均为60℃,与同类酶相比具有一定的优势;PA1及PA2的温度稳定性较好,50℃下酶活保持稳定,60℃下处理10 min,二者均保持70%左右的酶活;PA1及PA2的pH耐受范围宽泛,在pH 4.0～...
Keyword ：
克隆表达 克隆表达 毕赤酵母 毕赤酵母 碱性蛋白酶 碱性蛋白酶 酶学性质 酶学性质
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| GB/T 7714 | 任雅馨 , 罗会颖 , 姚斌 et al. Cordyceps fumosorosea和Beauveria bassiana来源的碱性蛋白酶在毕赤酵母中的异源表达与性质测定 [J]. | 中国农业科技导报 , 2020 , 22 (11) : 69-78 . |
| MLA | 任雅馨 et al. "Cordyceps fumosorosea和Beauveria bassiana来源的碱性蛋白酶在毕赤酵母中的异源表达与性质测定" . | 中国农业科技导报 22 . 11 (2020) : 69-78 . |
| APA | 任雅馨 , 罗会颖 , 姚斌 , 王国增 , 涂涛 . Cordyceps fumosorosea和Beauveria bassiana来源的碱性蛋白酶在毕赤酵母中的异源表达与性质测定 . | 中国农业科技导报 , 2020 , 22 (11) , 69-78 . |
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Many methods have been used to detect heavy metals in herbal medicines, while few are developed to remove them. In this study, a novel genetically engineered fusion protein composed of metallothionein (MT), cellulose binding module (CBM), and superfolder GFP (sfGFP) was designed to remove heavy metals. MT, a kind of cysteine-rich protein, was used to chelate heavy metals with high specific affinity. The CBM facilitated the fusion protein MT-CBM-sfGFP binding to cellulose specifically, which made the purification and immobilization in one step. The sfGFP was used to detect the fusion protein MT-CBM-sfGFP easily during the process of expression and immobilization. The MT from Cancer pagurus (MTCap) and the CBM from Cellulomonas fimi (CBMcef) were used as an example and the fusion protein (MTCap-CBMcef-sfGFP) was expressed in Escherichia coli. Then, the cell lysates were mechanically mixed with cellulose to create biosorbent MTCap-CBMcef-sfGFP@cellulose. The efficiency of the biosorbent MTCap-CBMcef-sfGFP@cellulose for Pb2+ removal was evaluated using the water decoction of Honeysuckle as a model. Results suggested that MTCap-CBMcef-sfGFP@cellulose had high efficiency for Pb2+ removal from the water decoction of Honeysuckle without affecting its active ingredients. The low-cost, easy production, and high efficiency of the biosorbent enable it to have many applications in heavy metal removal from aqueous solutions of herbal medicines and food.
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| GB/T 7714 | Xiao, Qing , Han, Jing , Jiang, Chang et al. Novel Fusion Protein Consisting of Metallothionein, Cellulose Binding Module, and Superfolder GFP for Lead Removal from the Water Decoction of Traditional Chinese Medicine [J]. | ACS OMEGA , 2020 , 5 (6) : 2893-2898 . |
| MLA | Xiao, Qing et al. "Novel Fusion Protein Consisting of Metallothionein, Cellulose Binding Module, and Superfolder GFP for Lead Removal from the Water Decoction of Traditional Chinese Medicine" . | ACS OMEGA 5 . 6 (2020) : 2893-2898 . |
| APA | Xiao, Qing , Han, Jing , Jiang, Chang , Luo, Meng , Zhang, Qingyi , He, Zhaodong et al. Novel Fusion Protein Consisting of Metallothionein, Cellulose Binding Module, and Superfolder GFP for Lead Removal from the Water Decoction of Traditional Chinese Medicine . | ACS OMEGA , 2020 , 5 (6) , 2893-2898 . |
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Proteinase K (PROK) from Parengyodontium album hydrolyzes keratin, a major protein component of poultry feathers, which are an inexpensive and renewable protein resource. Based on structural studies for analysis of amino acid flexibility near the catalytic center, identification of highly conserved residues, and experimental screening, we obtained a mutant R218S with residual activity 1.6-fold higher than that of PROK after incubation at 60 degrees C for 1 h. Molecular dynamics simulation indicated that substitution of Arg218 with Ser leads to three hydrogen bonds being introduced into the structure, stabilizing the beta-sheet in which Ser218 is located, and thus improvement of thermostability. Additionally, the mutant R218S had a 15% increase in specific activity compared to PROK and improvement in the rate and thoroughness of feather degradation compared with PROK. We confirmed the positive effects of enhancing catalytic center rigidity on enzyme thermostability, a finding which may have broad applications. (C) 2019 Elsevier B.V. All rights reserved.
Keyword ：
B-factor B-factor Feather degradation Feather degradation Molecular modification Molecular modification Proteinase K Proteinase K Thermostability Thermostability
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| GB/T 7714 | Ren, Yaxin , Luo, Huiying , Huang, Huoqing et al. Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 154 : 1586-1595 . |
| MLA | Ren, Yaxin et al. "Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 154 (2020) : 1586-1595 . |
| APA | Ren, Yaxin , Luo, Huiying , Huang, Huoqing , Hakulinen, Nina , Wang, Yaru , Wang, Yuan et al. Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2020 , 154 , 1586-1595 . |
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A new pullulanase-encoding gene, pLdSL3, was cloned from Alkalibacterium sp. SL3 and expressed in Escherichia coil. Deduced PulSL3 contains 2026 amino acid residues and consists of a putative signal peptide, four carbohydrate-binding modules, an E_set pullulanase domain, an alpha-amylase domain, and a pullulanase domain. The purified rPulSL3 exhibited pullulanase activity only. To identify the functions of the alpha-amylase and pullulanase domains, the N- and C-terminus truncated mutants (pulSL3 Delta N and pulSL3 Delta C) were also expressed in E. coli. The purified rPulSL3 Delta N had pullulanase activity, while rPulSL3 Delta C had no either alpha-amylase or pullulanase activity. Both rPulSL3 and rPulSL3 Delta N exhibited maximum activities at pH 9.0 and 50 degrees C, and were highly active and stable at the concentration of NaCl up to 4 M and resistant to a few surfactants and detergents. In comparison to the wild type, rPulSL3 Delta N had better thermostability (retaining 62.9 vs. 33.6% activity at 52 degrees C for 30 min), higher substrate affinity (with K-m of 0.10 vs. 0.15 mg mL(-1)) and higher catalytic efficiency (836.6 vs. 520.3 mL mg(-1) s(-1)). These excellent properties make rPulSL3 Delta N potential for the application in the industries of detergent, food, and environmental remediation.
Keyword ：
Alkalibacterium Alkalibacterium Alkaline Alkaline Halotolerant Halotolerant Pullulanase Pullulanase SDS resistant SDS resistant Truncated mutant Truncated mutant
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| GB/T 7714 | Huang, Han , Lin, Yun , Wang, Guozeng et al. Gene cloning, expression and biochemical characterization of a new multi-domain, halotolerant and SDS-resistant alkaline pullulanase from Alkalibacterium sp. SL3 [J]. | PROCESS BIOCHEMISTRY , 2020 , 96 : 1-10 . |
| MLA | Huang, Han et al. "Gene cloning, expression and biochemical characterization of a new multi-domain, halotolerant and SDS-resistant alkaline pullulanase from Alkalibacterium sp. SL3" . | PROCESS BIOCHEMISTRY 96 (2020) : 1-10 . |
| APA | Huang, Han , Lin, Yun , Wang, Guozeng , Lin, Juan . Gene cloning, expression and biochemical characterization of a new multi-domain, halotolerant and SDS-resistant alkaline pullulanase from Alkalibacterium sp. SL3 . | PROCESS BIOCHEMISTRY , 2020 , 96 , 1-10 . |
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ObjectiveTo explore the diversity of glycoside hydrolase family 10 xylanase genes in the sediment of soda lake Dabusu by using high-throughput amplicon sequencing based on the Illumina HiSeq2500 platform.ResultsA total of 227,420 clean reads, representing approximately 49.5Mbp, were obtained. Operational taxonomic unit (OTU) classification, with a 95% sequence identity cut-off, resulted in 467 OTUs with 392 annotated as GH10 xylanase, exhibiting 35-99% protein sequence identity with their closest-related xylanases in GenBank. Above 75% of the total OTUs demonstrated less than 80% identity with known xylanases. In addition, xylanases derived from the sediment were found to be affiliated to 12 different phyla, with Bacteroidetes, Proteobacteria, Actinobacteria, Firmicutes, Verrucomicrobia, and Basidiomycota being the dominant phyla. Moreover, barcode sequence had a major effect on abundance with only a minor effect on diversity.ConclusionsHigh-throughput amplicon sequencing offers insight into xylanase gene diversity at a substantially higher resolution and lesser cost than library cloning and Sanger sequencing, facilitating a more thorough understanding of xylanase distribution and ecology.
Keyword ：
Amplicon sequencing Amplicon sequencing Gene diversity Gene diversity High-throughput High-throughput Metagenomics Metagenomics Soda lake Soda lake Xylanase Xylanase
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| GB/T 7714 | Wang, Guozeng , Ren, Yaxin , Ng, Tzi Bun et al. High-throughput amplicon sequencing demonstrates extensive diversity of xylanase genes in the sediment of soda lake Dabusu [J]. | BIOTECHNOLOGY LETTERS , 2019 , 41 (3) : 409-418 . |
| MLA | Wang, Guozeng et al. "High-throughput amplicon sequencing demonstrates extensive diversity of xylanase genes in the sediment of soda lake Dabusu" . | BIOTECHNOLOGY LETTERS 41 . 3 (2019) : 409-418 . |
| APA | Wang, Guozeng , Ren, Yaxin , Ng, Tzi Bun , Streit, Wolfgang R. , Ye, Xiuyun . High-throughput amplicon sequencing demonstrates extensive diversity of xylanase genes in the sediment of soda lake Dabusu . | BIOTECHNOLOGY LETTERS , 2019 , 41 (3) , 409-418 . |
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