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学者姓名：林峻
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Abstract ：
Trauma is a significant health issue that not only leads to immediate death in many cases but also causes severe complications, such as sepsis, thrombosis, haemorrhage, acute respiratory distress syndrome and traumatic brain injury, among trauma patients. Target protein identification technology is a vital technique in the field of biomedical research, enabling the study of biomolecular interactions, drug discovery and disease treatment. It plays a crucial role in identifying key protein targets associated with specific diseases or biological processes, facilitating further research, drug design and the development of treatment strategies. The application of target protein technology in biomarker detection enables the timely identification of newly emerging infections and complications in trauma patients, facilitating expeditious medical interventions and leading to reduced post-trauma mortality rates and improved patient prognoses. This review provides an overview of the current applications of target protein identification technology in trauma-related complications and provides a brief overview of the current target protein identification technology, with the aim of reducing post-trauma mortality, improving diagnostic efficiency and prognostic outcomes for patients.
Keyword ：
biomarkers biomarkers mass spectrometry mass spectrometry target protein identification target protein identification trauma trauma trauma-related complications trauma-related complications
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| GB/T 7714 | Wei, YiLiu , Ren, Xiaohan , Yuan, Zhitao et al. Trauma diagnostic-related target proteins and their detection techniques [J]. | EXPERT REVIEWS IN MOLECULAR MEDICINE , 2024 , 26 . |
| MLA | Wei, YiLiu et al. "Trauma diagnostic-related target proteins and their detection techniques" . | EXPERT REVIEWS IN MOLECULAR MEDICINE 26 (2024) . |
| APA | Wei, YiLiu , Ren, Xiaohan , Yuan, Zhitao , Hong, Jie , Wang, Tao , Chen, Weizhi et al. Trauma diagnostic-related target proteins and their detection techniques . | EXPERT REVIEWS IN MOLECULAR MEDICINE , 2024 , 26 . |
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Background Nasopharyngeal carcinoma (NPC) treatment is largely based on a 'one-drug-fits-all' strategy in patients with similar pathological characteristics. However, given its biological heterogeneity, patients at the same clinical stage or similar therapies exhibit significant clinical differences. Thus, novel molecular subgroups based on these characteristics may better therapeutic outcomes. Methods Herein, 192 treatment-naive NPC samples with corresponding clinicopathological information were obtained from Fujian Cancer Hospital between January 2015 and January 2018. The gene expression profiles of the samples were obtained by RNA sequencing. Molecular subtypes were identified by consensus clustering. External NPC cohorts were used as the validation sets. Results Patients with NPC were classified into immune, metabolic, and proliferative molecular subtypes with distinct clinical features. Additionally, this classification was repeatable and predictable as validated by the external NPC cohorts. Metabolomics has shown that arachidonic acid metabolites were associated with NPC malignancy. We also identified several key genes in each subtype using a weighted correlation network analysis. Furthermore, a prognostic risk model based on these key genes was developed and was significantly associated with disease-free survival (hazard ratio, 1.11; 95% CI, 1.07-1.16; P < 0.0001), which was further validated by an external NPC cohort (hazard ratio, 7.71; 95% CI, 1.39-42.73; P < 0.0001). Moreover, the 1-, 3-, and 5-year areas under the curve were 0.84 (95% CI, 0.74-0.94), 0.81 (95% CI, 0.73-0.89), and 0.82 (95% CI, 0.73-0.90), respectively, demonstrating a high predictive value. Conclusions Overall, we defined a novel classification of nasopharyngeal carcinoma (immune, metabolism, and proliferation subtypes). Among these subtypes, metabolism and proliferation subtypes were associated with advanced stage and poor prognosis of NPC patients, whereas the immune subtype was linked to early stage and favorable prognosis.
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| GB/T 7714 | Lin, Wanzun , Chen, Xiaochuan , Huang, Zongwei et al. Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma [J]. | BRITISH JOURNAL OF CANCER , 2024 , 130 (7) : 1176-1186 . |
| MLA | Lin, Wanzun et al. "Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma" . | BRITISH JOURNAL OF CANCER 130 . 7 (2024) : 1176-1186 . |
| APA | Lin, Wanzun , Chen, Xiaochuan , Huang, Zongwei , Ding, Qin , Yang, Hanxuan , Li, Ying et al. Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma . | BRITISH JOURNAL OF CANCER , 2024 , 130 (7) , 1176-1186 . |
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Here, the mixed metal nodes MOFs, Pd/MIL-100(FeaCub), were constructed as photocatalysts for hydrogenation of alpha,beta-unsaturated aldehyde (UAL) under visible light. 1 wt% Pd/MIL-100(Fe0.81Cu0.19) can convert a range of UAL to saturated aldehydes (SAL) with a high efficiency (approximate to 100 %) and selectivity (approximate to 98 %). The results of XPS and in situ DRIFTS reveal that UAL can be selectively activated via a coordination of -C--C- on Cu2+ sites, determining the high selectivity of the photocatalytic reaction. The mixed metal nodes and Pd clusters can improve the transformation and separation of photogenerated electrons-holes. EPR result suggests that photogenerated carriers can facilitate the generation of H center dot on Pd/MIL-100(Fe0.81Cu0.19), enhancing the catalytic activity. A possible mechanism is proposed for elucidating the catalytic processes at the molecular level. This work provides a valuable strategy for tailoring the selectivity of photocatalytic hydrogenation via selective coordination activation.
Keyword ：
alpha alpha beta-Unsaturated aldehydes beta-Unsaturated aldehydes Coordination activation Coordination activation Hydrogenation Hydrogenation MIL-100(Fe/Cu) MIL-100(Fe/Cu) Photocatalyst Photocatalyst
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| GB/T 7714 | Wang, Zhiwen , Kang, Yueyue , Shi, Yingzhang et al. Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub) [J]. | APPLIED CATALYSIS B-ENVIRONMENTAL , 2024 , 340 . |
| MLA | Wang, Zhiwen et al. "Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub)" . | APPLIED CATALYSIS B-ENVIRONMENTAL 340 (2024) . |
| APA | Wang, Zhiwen , Kang, Yueyue , Shi, Yingzhang , Liu, Cheng , Liu, Yunni , Lin, Jun et al. Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub) . | APPLIED CATALYSIS B-ENVIRONMENTAL , 2024 , 340 . |
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Glioma stem cells (GSCs) are thought to be responsible for the initiation and progression of glioblastoma (GBM). GBM presents highly invasive growth with a very high recurrence rate, so it has become a clinical problem to be solved urgently. RNAseq demonstrates that thrombospondin 1 (THBS1) acts not only in the angiogenic core of glioma but also with a high degree of invasiveness and infiltration. Nevertheless, defects in the signaling pathway research lead to a poor prognosis in glioma patients. To investigate the relevant molecular mechanism and signal pathway of glioma stem cell behavior mediated by THBS1, U251 astroglioma cells and GSCs were taken as model cells for in vitro experiments. The biological effects of THBS1 on glioma proliferation, migration, and adhesion were evaluated using Cell Counting Kit-8(CCK8) assays, EdU incorporation assays, migration assays, Transwell assays, Western blotting, and RNAseq. We found that the knockout of the THBS1 gene by CRISPR/Cas9 promoted proliferation and migration in U251 cells and GSCs, as well as influencing cell cycle progression by regulating the TNF/MAPK/NF-kappa B and TGF-beta/Smad signaling pathways. Moreover, U251 cells and GSCs showed different re-sponses to THBS1 knockout, suggesting specific and potential targets for GSCs in signaling pathways mediated by THBS1.
Keyword ：
Glioma stem cells Glioma stem cells MAPK MAPK Proliferation Proliferation ? signaling pathways ? signaling pathways TGF TGF Thrombospondin 1 Thrombospondin 1 TNF TNF TRAF2 TRAF2
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| GB/T 7714 | Chen, Liqun , Fang, Wei , Chen, Weizhi et al. Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells [J]. | CELLULAR SIGNALLING , 2023 , 106 . |
| MLA | Chen, Liqun et al. "Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells" . | CELLULAR SIGNALLING 106 (2023) . |
| APA | Chen, Liqun , Fang, Wei , Chen, Weizhi , Wei, Yiliu , Ding, Jinwang , Li, Jiafeng et al. Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells . | CELLULAR SIGNALLING , 2023 , 106 . |
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本发明公开了一种丝状真菌复制子，所述复制子的核苷酸序列如SEQ ID NO.2所示。所述复制子是以19种真菌基因组为模板构建宏基因组文库，转化黑曲霉孢子筛选得到，来自厚孢根霉内生菌伯克霍尔德氏菌HKI454基因组。本发明的复制子可以在黑曲霉表达系统中发挥复制功能，并且可以提供比AMA1复制子更高的稳定性，为首次在除构巢曲霉以外的真菌中发现的复制子，丰富了复制子元件库，拓展了丝状真菌利用复制子的选择多样性。
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| GB/T 7714 | 林峻 , 燕天鹤 . 一种丝状真菌复制子 : CN202210598346.0[P]. | 2022-05-30 00:00:00 . |
| MLA | 林峻 et al. "一种丝状真菌复制子" : CN202210598346.0. | 2022-05-30 00:00:00 . |
| APA | 林峻 , 燕天鹤 . 一种丝状真菌复制子 : CN202210598346.0. | 2022-05-30 00:00:00 . |
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本发明公开了一种可规模化生产具有活性的DNA聚合酶的方法。所述方法包括以下步骤：1)将携带有目的基因的菌种划线于带氨苄抗性的LB平板上，挑取单菌落培养后作为种子液；2)配置高密度发酵培养基，加入发酵罐中；3)将种子液转接到步骤2)的发酵罐中进行培养；4)当培养至溶氧下降至30%以下时，开始加入补料培养基，控制溶氧为30％以上；5)当培养至细菌菌体湿重距最大菌体湿重4g/L时，一次性加入IPTG诱导，使IPTG终浓度为1±0.1 mM；6)达到最佳诱导时长后停止发酵，放罐，获取菌体。本发明提高了目标产物的发酵稳定性，并且减少了包涵体的产生，可实现规模化生产DNA聚合酶。
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| GB/T 7714 | 陈鲤群 , 高上 , 袁智涛 et al. 一种可规模化生产具有活性的DNA聚合酶的方法 : CN202210959921.5[P]. | 2022-08-11 00:00:00 . |
| MLA | 陈鲤群 et al. "一种可规模化生产具有活性的DNA聚合酶的方法" : CN202210959921.5. | 2022-08-11 00:00:00 . |
| APA | 陈鲤群 , 高上 , 袁智涛 , 王涛 , 姜文倩 , 林峻 . 一种可规模化生产具有活性的DNA聚合酶的方法 : CN202210959921.5. | 2022-08-11 00:00:00 . |
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本实用新型公开一种采样管定位结构及样品管架，包括样品管放置孔，样品管放置孔上端面的外围设有至少三个沿圆周方向间隔设置的导向棱，各导向棱的凸起面为导向坡面，且导向坡面的低端朝向样品管放置孔。本实用新型采用多个导向棱的结构，用于样品管自动定圆心。此结构定圆心简单快速，从根源解决了定圆心问题。
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| GB/T 7714 | 林峻 , 高上 , 袁智涛 . 一种采样管定位结构及样品管架 : CN202320287361.3[P]. | 2023-02-22 00:00:00 . |
| MLA | 林峻 et al. "一种采样管定位结构及样品管架" : CN202320287361.3. | 2023-02-22 00:00:00 . |
| APA | 林峻 , 高上 , 袁智涛 . 一种采样管定位结构及样品管架 : CN202320287361.3. | 2023-02-22 00:00:00 . |
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Banknotes have long been suspected to be biologically "dirty" due to their frequent human contact, which may transmit human microbial pathogens. Still, it is an unsettled issue whether the microbes on banknotes pose a real threat to human health. In several previous studies, metagenomic sequencing was used to reveal the diversities of microbes on banknotes but live microorganism culture and functional verification were lacking. In this study, we collected banknotes of RMB in China as well as dollar bills in the United States and analyzed the microbial biodiversity and drug resistance genes carried by the identified microbes by metagenomic sequencing and in vitro culture methods. We identified eight major genera of drug-resistant bacteria through screening of 30 antibiotics, and the blood agar plate culture uncovered six pathogenic fungal species. Numerous phage and six dangerous viral sequences were also found. These results should substantiate our concern about the potential risk of banknotes to human health.
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| GB/T 7714 | Lin, Jun , Jiang, Wenqian , Shi, Yang et al. Metagenomic Sequencing Revealed the Potential Pathogenic Threats of Banknotes [J]. | ACS OMEGA , 2021 , 6 (5) : 3499-3507 . |
| MLA | Lin, Jun et al. "Metagenomic Sequencing Revealed the Potential Pathogenic Threats of Banknotes" . | ACS OMEGA 6 . 5 (2021) : 3499-3507 . |
| APA | Lin, Jun , Jiang, Wenqian , Shi, Yang , Cai, Weiwen . Metagenomic Sequencing Revealed the Potential Pathogenic Threats of Banknotes . | ACS OMEGA , 2021 , 6 (5) , 3499-3507 . |
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定向进化是一个循环过程,在构建多样化基因序列和筛选功能基因变体之间交替进行。该技术目前已被广泛应用于DNA序列、基因功能和蛋白质结构的优化和分析。定向进化包括随机基因文库的生成、基因在合适宿主中的表达和突变文库的筛选。构建基因文库的关键是库容量和突变多样性,而筛选变体的关键是高灵敏度和高通量。文中讨论了定向进化技术的最新进展,这些新技术极大地加速和简化了传统的定向进化过程,为定向进化的发展注入了强劲动力。
Keyword ：
半理性设计 半理性设计 定向进化 定向进化 突变文库 突变文库 随机突变 随机突变 高通量筛选 高通量筛选
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| GB/T 7714 | 陈珏 , 黄佳敏 , 燕天鹤 et al. 随机突变文库构建与筛选研究进展 [J]. | 生物工程学报 , 2021 , 37 (01) : 163-177 . |
| MLA | 陈珏 et al. "随机突变文库构建与筛选研究进展" . | 生物工程学报 37 . 01 (2021) : 163-177 . |
| APA | 陈珏 , 黄佳敏 , 燕天鹤 , 彭晓宇 , 林峻 . 随机突变文库构建与筛选研究进展 . | 生物工程学报 , 2021 , 37 (01) , 163-177 . |
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Traditional pathogenic diagnosis presents defects such as a low positivity rate, inability to identify uncultured microorganisms, and time-consuming nature. Clinical metagenomics next-generation sequencing can be used to detect any pathogen, compensating for the shortcomings of traditional pathogenic diagnosis. We report third-generation long-read sequencing results and second-generation short-read sequencing results for ascitic fluid from a patient with liver ascites and compared the two types of sequencing results with the results of traditional clinical microbial culture. The distribution of pathogenic microbial species revealed by the two types of sequencing results was quite different, and the third-generation sequencing results were consistent with the results of traditional microbial culture, which can effectively guide subsequent treatment. Short reads, the lack of amplification, and enrichment to amplify signals from trace pathogens, and host background noise may be the reasons for the high error in the second-generation short-read sequencing results. Therefore, we propose that long-read-based rRNA analysis technology is superior to the short-read shotgun-based metagenomics method in the identification of pathogenic bacteria.
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| GB/T 7714 | Yu, Xiaoling , Jiang, Wenqian , Huang, Xinhui et al. rRNA Analysis Based on Long-Read High-Throughput Sequencing Reveals a More Accurate Diagnostic for the Bacterial Infection of Ascites [J]. | BIOMED RESEARCH INTERNATIONAL , 2021 , 2021 . |
| MLA | Yu, Xiaoling et al. "rRNA Analysis Based on Long-Read High-Throughput Sequencing Reveals a More Accurate Diagnostic for the Bacterial Infection of Ascites" . | BIOMED RESEARCH INTERNATIONAL 2021 (2021) . |
| APA | Yu, Xiaoling , Jiang, Wenqian , Huang, Xinhui , Lin, Jun , Ye, Hanhui , Liu, Baorong . rRNA Analysis Based on Long-Read High-Throughput Sequencing Reveals a More Accurate Diagnostic for the Bacterial Infection of Ascites . | BIOMED RESEARCH INTERNATIONAL , 2021 , 2021 . |
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