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学者姓名：严芬
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Abstract ：
In view of the biological significance and micro-heterogeneity of protein glycosylation for human health, specific enrichment of N-glycosylated proteins/peptides from complex biological samples is a prerequisite for the discovery of disease biomarkers and clinical diagnosis. In this work, we propose a "grafting-from" N-glycoprotein enriching method based on the in-situ growth of thermoresponsive polymer brushes from the N-glycosylated site of proteins. The initiator was first attached to the pre-oxidized glycan moieties by hydrazide chemistry, from which the thermoresponsive polymers can be grown to form giant protein-polymer conjugates (PPC). The thermosensitive PPC can be precipitated and separated by raising the temperature to above its lower critical solubility temperature (LCST). Mass spectrometry verified 210 N-glycopeptides corresponding to 136 N-glycoproteins in the rabbit serum. These results demonstrate the capability of the tandem thermoprecipitation strategy to enrich and separate N-glycoprotein/glycopeptide. Due to its simplicity and efficiency specifically, this method holds the potential for identifying biomarkers from biological samples in N-glycoproteome analysis.
Keyword ：
Glycan-selective Glycan-selective glycopeptide glycopeptide N-Glycoprotein N-Glycoprotein Thermoprecipitation Thermoprecipitation
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| GB/T 7714 | Shu, Jingjing , Xiong, Wenli , Zhang, Ran et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides [J]. | TALANTA , 2023 , 253 . |
| MLA | Shu, Jingjing et al. "Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides" . | TALANTA 253 (2023) . |
| APA | Shu, Jingjing , Xiong, Wenli , Zhang, Ran , Ma, Shanyun , Zhou, Kaiqiang , Wang, Xuwei et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides . | TALANTA , 2023 , 253 . |
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Non-alcoholic fatty liver disease (NAFLD) has become a globally rising issue that can cause liver-related morbidity and mortality. Recent studies have revealed that a high-fat diet (HFD) highly contributes to the prevalence and progression of NAFLD via impacting gut microbiota and lipid metabolism signal pathways. Resveratrol (RSV), a natural bioactive compound, has exhibited potential for preventing and alleviating NAFLD. However, due to the poor bioavailability of RSV, its strengths and underlying mechanisms for NAFLD therapeutic potential are poorly understood. To address this, we utilized Hydroxypropyl-beta-Cyclodextrin (HBC) to encapsulate RSV to enhance its water solubility and conducted prevention and intervention experiments in HFDfed mice. The results showed that the HBC has significantly enhanced the water solubility of RSV by 250-fold and the HBC-RSV better prevented and alleviated the liver steatosis, obesity and abnormal lipid metabolism induced by HFD than RSV alone. Meanwhile, combining HFD and HBC-RSV or RSV prevented HFD mice progressing to NAFLD. Besides, further investigation indicated that RSV could resist liver injury and obesity by modulating gut microbiota, raising the levels of short-chain fatty acids (SCFAs) and activating AMP-activated protein kinase (AMPK) signaling pathway. The activated pathway down-regulated sterol receptor element binding protein 1c (SREBP1c) and acetyl-coenzyme A carboxylase (ACC) to decrease lipid synthesis and up-regulated peroxisome proliferators-activated receptor & alpha; (PPAR & alpha;) to promote the fatty acid oxidation, thus preventing NAFLD. Our findings suggested that water solubility-enhanced RSV beneficially modulated gut microbiota, altered gut microbiota-derived SCFAs, and activated lipid metabolism regulatory pathways, providing potential for NAFLD prevention and alleviation.
Keyword ：
AMPK AMPK Gut microbiota Gut microbiota Hydroxypropyl-beta-Cyclodextrin Hydroxypropyl-beta-Cyclodextrin NAFLD NAFLD resveratrol resveratrol SCFAs SCFAs
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| GB/T 7714 | Ke, Wenya , Huang, Juan , Zhong, Yi et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway [J]. | FOOD BIOSCIENCE , 2023 , 54 . |
| MLA | Ke, Wenya et al. "Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway" . | FOOD BIOSCIENCE 54 (2023) . |
| APA | Ke, Wenya , Huang, Juan , Zhong, Yi , Shi, Yuhong , Yan, Fen , Huang, Da et al. Hydroxypropyl-beta-Cyclodextrin embedded resveratrol regulates gut microbiota to prevent NAFLD via activating AMPK signaling pathway . | FOOD BIOSCIENCE , 2023 , 54 . |
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Bacterial infection of wounds is one of great concern to patients, and rapid and correct detection of bacterial infection is crucial to ensure accurate diagnosis and early intervention. Based on the principle that glucose can only be consumed by live bacteria, a fluorescent biosensor was constructed to detect four kinds of common infectious bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in skin wounds, taking advantage of magnetic bead-aptamer for recognizing, sorting and enrichment, platinum nanoparticles for signal amplification. The linear detection range of AB, EC, PA and SA were 27- 8 x 106 CFU/mL, 10- 2.5 x 107 CFU/mL, 34- 2.5 x 107 CFU/mL and 10- 1.0 x 107 CFU/mL, respectively, and the limits of detection were 27 CFU/mL, 10 CFU/mL, 34 CFU/mL and 2 CFU/mL. Furthermore, all target bacteria in samples containing 8 x 108 CFU/mL of other interfering bacteria have been successfully identified and quantified. The proposed method was also successfully applied to the detection of bacterial infection in skin wounds of mouse and human, including the detection of separate bacterial infection as well as a coinfection. The recovery of this method was in the range of 90.161- 109.961%. Thus, this proposed method can be a promising candidate for rapid and convenient evaluation of infectious bacteria in point-of-care settings.
Keyword ：
Aptamer Aptamer Bacterial infection Bacterial infection Fluorescent biosensor Fluorescent biosensor Platinum nanoparticles Platinum nanoparticles Wound Wound
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| GB/T 7714 | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
| MLA | Gao, Lanmei et al. "Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds" . | SENSORS AND ACTUATORS B-CHEMICAL 393 (2023) . |
| APA | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong , Zhong, Yi , Jiang, Linhai , Wu, Yuanzi et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
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本发明涉及一种忧遁草抗氧化十三肽及其制备方法和应用，属于生物技术领域。所述抗氧化肽氨基酸序列为LLPENDPSANHLM，该抗氧化肽的制备方法是以忧遁草叶为原料，采用碱溶酸沉淀法提取粗制品，再经分离纯化、鉴定及合成技术得到所述抗氧化肽。药理实验证明，本发明提供的忧遁草抗氧化十三肽能在不同程度上保护HepG‑2细胞免受H2O2毒害，其具有结构简单、抗氧化活力强、安全性高的特点，可以作为化学合成抗氧化剂的优良替代。本发明也为食品、药品及化妆品行业研发新型天然添加剂提供了理论依据和实践参考。
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| GB/T 7714 | 严芬 , 刘文静 , 张少龙 et al. 一种忧遁草抗氧化十三肽及其制备方法和应用 : CN202210288893.9[P]. | 2022-03-23 00:00:00 . |
| MLA | 严芬 et al. "一种忧遁草抗氧化十三肽及其制备方法和应用" : CN202210288893.9. | 2022-03-23 00:00:00 . |
| APA | 严芬 , 刘文静 , 张少龙 , 费庆彬 . 一种忧遁草抗氧化十三肽及其制备方法和应用 : CN202210288893.9. | 2022-03-23 00:00:00 . |
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本发明公开一株用于香蕉采后病害的酵母菌P4及其使用方法。所述酵母菌P4在中国微生物菌种保藏管理委员会普通微生物中心的保藏编号为CGMCC No.22561。所述酵母菌P4的使用方法为：菌株活化，菌体用无菌水配成1×108 cells/mL的菌悬液；将菌悬液喷洒于香蕉表面，自然晾干后，放入0.015 mm厚的聚乙烯薄膜袋中，恒温恒湿贮藏。所述酵母菌P4可以控制香蕉炭疽病，同时该菌株抑菌谱较广，可以控制黄曲霉、拟盘多毛孢属、枇杷胶孢炭疽菌、香蕉镰刀菌等。
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| GB/T 7714 | 严芬 , 吴芸芸 , 蔡婷 et al. 一株控制香蕉采后病害的酵母菌P4及其使用方法 : CN202111008228.1[P]. | 2021-08-31 00:00:00 . |
| MLA | 严芬 et al. "一株控制香蕉采后病害的酵母菌P4及其使用方法" : CN202111008228.1. | 2021-08-31 00:00:00 . |
| APA | 严芬 , 吴芸芸 , 蔡婷 , 刘文静 , 张帆 . 一株控制香蕉采后病害的酵母菌P4及其使用方法 : CN202111008228.1. | 2021-08-31 00:00:00 . |
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本发明涉及一种重组褐藻胶裂解酶AlyL7及其应用，所述褐藻胶裂解酶的氨基酸序列如SEQ ID NO.1所示，其编码基因的核苷酸序列如SEQ ID NO.2所示。所述重组褐藻胶裂解酶属于双功能型褐藻胶裂解酶，其最适反应温度为40℃，最适反应pH为9.0，在0‑25℃、pH 5.0‑10.0的条件时较稳定，对海藻酸钠、聚古洛糖醛酸、聚甘露糖醛酸均具有降解活性。所述重组褐藻胶裂解酶也属于内切酶，其酶解海藻酸钠的终产物包括不饱和1‑5糖，以不饱和2‑3糖为主。所述褐藻胶裂解酶反应速度快，效率高，具有良好的工业化应用潜质。
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| GB/T 7714 | 严芬 , 陈骏颖 , 钟金福 et al. 一种重组褐藻胶裂解酶AlyL7及其应用 : CN202111240122.4[P]. | 2021-10-25 00:00:00 . |
| MLA | 严芬 et al. "一种重组褐藻胶裂解酶AlyL7及其应用" : CN202111240122.4. | 2021-10-25 00:00:00 . |
| APA | 严芬 , 陈骏颖 , 钟金福 , 张少龙 . 一种重组褐藻胶裂解酶AlyL7及其应用 : CN202111240122.4. | 2021-10-25 00:00:00 . |
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本发明公开了一株抑制香蕉采后病害的贝莱斯芽孢杆菌LZT14及其应用，属于植物采后病害防治技术领域。所述菌株从福州大学荔枝树周围土壤样品中分离筛选得到，已于2021年8月16日保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.23150。该菌株抑菌谱广、且可应用于香蕉采后病害预防和贮藏保鲜。该菌株的使用方法为：菌株活化，洗涤后重悬；将菌悬液喷洒于香蕉表面，风干，放入0.015 mm厚的聚乙烯薄膜袋中，恒温恒湿贮藏。香蕉体内试验结果表明，在采后28℃，80%相对湿度环境中储藏时，该菌株可以明显降低香蕉的病情指数，生防应用前景良好。
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| GB/T 7714 | 严芬 , 刘聪 , 陈晓杰 et al. 抑制香蕉采后病害的贝莱斯芽孢杆菌LZT14及其应用 : CN202111586238.3[P]. | 2021-12-23 00:00:00 . |
| MLA | 严芬 et al. "抑制香蕉采后病害的贝莱斯芽孢杆菌LZT14及其应用" : CN202111586238.3. | 2021-12-23 00:00:00 . |
| APA | 严芬 , 刘聪 , 陈晓杰 , 吴崟沣 , 方婷 . 抑制香蕉采后病害的贝莱斯芽孢杆菌LZT14及其应用 : CN202111586238.3. | 2021-12-23 00:00:00 . |
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本发明涉及一种具有抗炎抗氧化活性的忧遁草多肽及其制备方法和应用，属于生物技术领域。所述忧遁草多肽序列的氨基酸序列为DKFEDNLYSAH，分子量为1338.2 Da。所述忧遁草多肽是以忧遁草为原料，采用超声辅助碱溶酸沉法获取忧遁草粗蛋白，再经分离纯化、鉴定、Fmoc固相合成得到的。试验证明，本发明所述忧遁草多肽具有很好的抗炎活性及抗氧化活性，具有很好的应用潜力和价值。
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| GB/T 7714 | 严芬 , 刘文静 , 刘聪 et al. 一种具有抗炎抗氧化活性的忧遁草多肽及其制备方法和应用 : CN202210458985.7[P]. | 2022-04-28 00:00:00 . |
| MLA | 严芬 et al. "一种具有抗炎抗氧化活性的忧遁草多肽及其制备方法和应用" : CN202210458985.7. | 2022-04-28 00:00:00 . |
| APA | 严芬 , 刘文静 , 刘聪 , 陈晓杰 , 张帆 . 一种具有抗炎抗氧化活性的忧遁草多肽及其制备方法和应用 : CN202210458985.7. | 2022-04-28 00:00:00 . |
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An abnormal microenvironment underlies poor healing in chronic diabetic chronic wounds. However, effectively modulating the microenvironment of the diabetic wound remains a great challenge due to sustained oxidative stress and chronic inflammation. Here, we present a unimolecular enzyme-polymer conjugate that demonstrates excellent multienzymatic cascade activities. The cascaded enzyme conjugates (CECs) were synthesized by grafting poly(N-acryloyl-lysine) (pLAAm) from the glycan moieties of glucose oxidase (GOx) via glycan-initiated polymerization. The resulting CECs exhibited multiple enzymatic properties of GOx, superoxide dismutase mimic, and catalase mimic activities simultaneously. The CECs facilitated the depletion of high blood glucose, ROS scavenging, bacteria-killing, anti-inflammatory effects, and sustained oxygen generation, which restored the microenvironment in diabetic wounds. In vivo results from a diabetic mouse model confirmed the capacity and efficiency of the cascade reaction for diabetic wound healing. Our findings demonstrate that the three-in-one enzyme-polymer conjugates alone can modulate the diabetic microenvironment for wound healing.
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| GB/T 7714 | Wu, Yuanzi , Lyu, Yanwei , Li, Ling et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing [J]. | BIOMACROMOLECULES , 2023 , 25 (1) : 43-54 . |
| MLA | Wu, Yuanzi et al. "Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing" . | BIOMACROMOLECULES 25 . 1 (2023) : 43-54 . |
| APA | Wu, Yuanzi , Lyu, Yanwei , Li, Ling , Zhou, Kaiqiang , Cai, Jingwen , Wang, Xuwei et al. Unimolecular Cascaded Multienzyme Conjugates Modulate the Microenvironment of Diabetic Wound to Promote Healing . | BIOMACROMOLECULES , 2023 , 25 (1) , 43-54 . |
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本专利公开了一种忧遁草的抗氧化十一肽及其制备方法和应用，所述抗氧化肽序列为DMGPPLSEKLH。本发明以忧遁草为原料，采用超声辅助碱溶酸提取技术进行制备，进一步通过离子色谱、反相高效液相色谱等分离纯化技术建立忧遁草抗氧化肽的纯化方法。体外抗氧化实验表明，所述忧遁草抗氧化十一肽可以有效清除DPPH、ABTS、羟基自由基等自由基，具有结构简单、抗氧化活力强、安全性高的特点，可以作为化学合成抗氧化剂的优良替代，而且具有一定的抗肿瘤活性，为食品、药品及化妆品行业研发新型天然添加剂提供了理论依据和实践参考。
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| GB/T 7714 | 严芬 , 刘文静 , 张丹 et al. 一种忧遁草抗氧化十一肽及其制备方法和应用 : CN202210103859.X[P]. | 2022-01-28 00:00:00 . |
| MLA | 严芬 et al. "一种忧遁草抗氧化十一肽及其制备方法和应用" : CN202210103859.X. | 2022-01-28 00:00:00 . |
| APA | 严芬 , 刘文静 , 张丹 , 胡诗琦 , 吴崟沣 . 一种忧遁草抗氧化十一肽及其制备方法和应用 : CN202210103859.X. | 2022-01-28 00:00:00 . |
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