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学者姓名：李建华
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Abstract ：
In view of the biological significance and micro-heterogeneity of protein glycosylation for human health, specific enrichment of N-glycosylated proteins/peptides from complex biological samples is a prerequisite for the discovery of disease biomarkers and clinical diagnosis. In this work, we propose a "grafting-from" N-glycoprotein enriching method based on the in-situ growth of thermoresponsive polymer brushes from the N-glycosylated site of proteins. The initiator was first attached to the pre-oxidized glycan moieties by hydrazide chemistry, from which the thermoresponsive polymers can be grown to form giant protein-polymer conjugates (PPC). The thermosensitive PPC can be precipitated and separated by raising the temperature to above its lower critical solubility temperature (LCST). Mass spectrometry verified 210 N-glycopeptides corresponding to 136 N-glycoproteins in the rabbit serum. These results demonstrate the capability of the tandem thermoprecipitation strategy to enrich and separate N-glycoprotein/glycopeptide. Due to its simplicity and efficiency specifically, this method holds the potential for identifying biomarkers from biological samples in N-glycoproteome analysis.
Keyword ：
Glycan-selective Glycan-selective glycopeptide glycopeptide N-Glycoprotein N-Glycoprotein Thermoprecipitation Thermoprecipitation
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| GB/T 7714 | Shu, Jingjing , Xiong, Wenli , Zhang, Ran et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides [J]. | TALANTA , 2023 , 253 . |
| MLA | Shu, Jingjing et al. "Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides" . | TALANTA 253 (2023) . |
| APA | Shu, Jingjing , Xiong, Wenli , Zhang, Ran , Ma, Shanyun , Zhou, Kaiqiang , Wang, Xuwei et al. Glycan-selective in-situ growth of thermoresponsive polymers for thermoprecipitation and enrichment of N-glycoprotein/glycopeptides . | TALANTA , 2023 , 253 . |
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Bacterial infection of wounds is one of great concern to patients, and rapid and correct detection of bacterial infection is crucial to ensure accurate diagnosis and early intervention. Based on the principle that glucose can only be consumed by live bacteria, a fluorescent biosensor was constructed to detect four kinds of common infectious bacteria (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in skin wounds, taking advantage of magnetic bead-aptamer for recognizing, sorting and enrichment, platinum nanoparticles for signal amplification. The linear detection range of AB, EC, PA and SA were 27- 8 x 106 CFU/mL, 10- 2.5 x 107 CFU/mL, 34- 2.5 x 107 CFU/mL and 10- 1.0 x 107 CFU/mL, respectively, and the limits of detection were 27 CFU/mL, 10 CFU/mL, 34 CFU/mL and 2 CFU/mL. Furthermore, all target bacteria in samples containing 8 x 108 CFU/mL of other interfering bacteria have been successfully identified and quantified. The proposed method was also successfully applied to the detection of bacterial infection in skin wounds of mouse and human, including the detection of separate bacterial infection as well as a coinfection. The recovery of this method was in the range of 90.161- 109.961%. Thus, this proposed method can be a promising candidate for rapid and convenient evaluation of infectious bacteria in point-of-care settings.
Keyword ：
Aptamer Aptamer Bacterial infection Bacterial infection Fluorescent biosensor Fluorescent biosensor Platinum nanoparticles Platinum nanoparticles Wound Wound
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| GB/T 7714 | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
| MLA | Gao, Lanmei et al. "Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds" . | SENSORS AND ACTUATORS B-CHEMICAL 393 (2023) . |
| APA | Gao, Lanmei , Zheng, Houbing , Hu, Yuanlong , Zhong, Yi , Jiang, Linhai , Wu, Yuanzi et al. Fluorescent biosensor based on MB-APT combined with Pt NPs for the detection of infectious bacteria in mouse and human wounds . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
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The combined contamination of multiple mycotoxins (MTs) is a matter of utmost concern, posing significant threats to both the economy stability and the well-being of humans and animals. Consequently, there is an urgent demand for highly sensitive and portable multiplex detection methods to effectively identify and quantify MTs. The primary objective of this paper is to provide a comprehensive overview of the methods to detect multiple MTs in agricultural products, including instrumental and biosensor approaches published in the last five years. Specifically, we emphasize detection methods for antibody (ab)- and aptamer (apt)-based biosensors, while undertaking a comparative analysis of their performance, particularly focusing on the sensitivity. Furthermore, this review proposes several promising technologies that can be leveraged in the future and outlines the challenges and prospects associated with achieving rapid, accurate, and intelligent detection of MTs.
Keyword ：
Agriculture products Agriculture products Antibody Antibody Aptamer Aptamer Multiplex detection Multiplex detection Mycotoxin Mycotoxin
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| GB/T 7714 | Wei, Chencheng , Wang, Handong , Li, Gaozheng et al. Multiplex detection methods for mycotoxins in agricultural products: A systematic review [J]. | FOOD CONTROL , 2023 , 158 . |
| MLA | Wei, Chencheng et al. "Multiplex detection methods for mycotoxins in agricultural products: A systematic review" . | FOOD CONTROL 158 (2023) . |
| APA | Wei, Chencheng , Wang, Handong , Li, Gaozheng , Li, Jianhua , Zhang, Fang , Wu, Yuezhong et al. Multiplex detection methods for mycotoxins in agricultural products: A systematic review . | FOOD CONTROL , 2023 , 158 . |
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The gelatin (GL) based film with high antioxidative and antibacterial activity and emulsifier-free was developed. The tensile strength (TS) and elongation at break (EAB) of gelatin films could be improved by adding TEMPO oxidized cellulose nanofibers (TCNFs). When the content of epigallocatechin gallate (EGCG) and carvacrol (CAR) reached 20 mg and 500 mg, the EAB of the composite film was 61.25%, which was much higher than that of gelatin film (28.73%). EGCG and CAR could improve UV barrier performance of GL/TCNFs films. GL/TCNFs/ 20EGCG/500CAR film allowed DPPH. and ABTS+ free radical scavenging rates to reach 92.6% and 90.6%, and the diameters of inhibition zones of E. coli and S. aureus were 22.28 mm and 42.58 mm, which showed that EGCG and CAR had synergistic effects. In the coating preservation of sea bass fillets, it also showed excellent inhibition of bacterial proliferation including Pseudomonas aeruginosa, H2S-producing bacteria as well as yeast and mold. TVB-N and TBA of control group were 15.21 mg/100g and 0.353 mg MDA/kg respectively on day 6, while lower values of 5.41 mg/100g and 0.124 mg MDA/kg respectively were recorded for GL/TCNFs/EGCG/500CAR group at the end of storage. All these results indicated that GL/TCNFs/EGCG/CAR film has potential application in fish preservation and is an environmentally friendly active food packaging system.
Keyword ：
Carvacrol Carvacrol Cellulose nanofiber Cellulose nanofiber Epigallocatechin gallate Epigallocatechin gallate Fish preservation Fish preservation Gelatin-based packaging film Gelatin-based packaging film
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| GB/T 7714 | Song, Gaojie , Lin, Sheng , Wu, Yuesheng et al. Emulsifier free fish gelatin based films with excellent antioxidative and antibacterial activity: Preparation, characterization and application in coating preservation of fish fillets [J]. | JOURNAL OF FOOD ENGINEERING , 2022 , 343 . |
| MLA | Song, Gaojie et al. "Emulsifier free fish gelatin based films with excellent antioxidative and antibacterial activity: Preparation, characterization and application in coating preservation of fish fillets" . | JOURNAL OF FOOD ENGINEERING 343 (2022) . |
| APA | Song, Gaojie , Lin, Sheng , Wu, Yuesheng , Shen, Jiaping , Wu, Jiulin , Zhu, Wenjin et al. Emulsifier free fish gelatin based films with excellent antioxidative and antibacterial activity: Preparation, characterization and application in coating preservation of fish fillets . | JOURNAL OF FOOD ENGINEERING , 2022 , 343 . |
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In this work, silver ion functionalized covalent organic polymer (COPTAPB-BPDA@SA@Ag+) was fabricated via the introduction of silver ion to COP, and utilized as selective sorbent for online SPME of unsaturated fatty acid methyl esters (UFAMEs). The synthesis of COPTAPB-BPDA@SA@Ag+ was confirmed by using the SEM, EDS, FT-IR, XRD and XPS characterization techniques. Due to the charge-transfer interaction between silver ion and C = C double bonds, COPTAPB-BPDA@SA@Ag+ displayed satisfactory extraction ability towards UFAMEs. Several SPME factors, such as ACN content in the sampling solution and eluent, sampling and elution flow rate, were investigated with respect to the extraction efficiency of UFAMEs. Under the optimized conditions, an online Ag+- SPME-Ag+-HPLC method for analysis of target trans-UFAMEs was proposed. A good linearity was established in the range of 0.04 ~ 2 mg/kg for two target trans-UFAMEs in milk tea samples. The limits of detection (LODs) and limits of quantitation (LOQs) were not more than 8.8 mu g/kg and 19 mu g/kg respectively, while the intra-day RSDs (n = 3) and the inter-day RSDs (n = 3) were less than 2.1% and 2.9% respectively. The recoveries for the proposed method was ranged from 82.3% to 93.5% with RSDs (n = 4) less than 5.9%. The above results demonstrated the selective extraction of COPTAPB-BPDA@SA@Ag+ towards UFAMEs from complex real samples, as well as the satisfactory detection sensitivity for trans-fatty acids of the proposed method. This study provided a facile approach for the preparation of metal ion functionalized COP sorbent and showed its wide potential for sample preparation.
Keyword ：
Covalent organic polymer Covalent organic polymer Milk tea Milk tea Online solid phase microextraction Online solid phase microextraction Silver ions Silver ions Trans-fatty acids Trans-fatty acids
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| GB/T 7714 | Wang, Jiabin , Sun, Jian , Xiao, Jianhua et al. Silver ion functionalized covalent organic polymer for selective online solid phase microextraction of unsaturated fatty acid methyl esters [J]. | MICROCHEMICAL JOURNAL , 2022 , 179 . |
| MLA | Wang, Jiabin et al. "Silver ion functionalized covalent organic polymer for selective online solid phase microextraction of unsaturated fatty acid methyl esters" . | MICROCHEMICAL JOURNAL 179 (2022) . |
| APA | Wang, Jiabin , Sun, Jian , Xiao, Jianhua , Fang, Xiuru , Li, Jianhua , Lin, Xucong . Silver ion functionalized covalent organic polymer for selective online solid phase microextraction of unsaturated fatty acid methyl esters . | MICROCHEMICAL JOURNAL , 2022 , 179 . |
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In this work, poly(vinylidene fluoride) (PVDF) membranes with hydrophilicity as well as preeminent mechanical strength and dye removal efficiency were fabricated by blending with three dimensional hydroxyapatite nanoparticles (HAPNPs). Surface chemical composition and morphology of the prepared membranes were systematically investigated by ATR-FTIR, XPS, XRD, FESEM, and EDS mapping analyses. The results verified that a large number of HAPNPs were successfully embedded on the modified membrane cross-sections. Moreover, HAPNPs content in the casting solution is an important factor that could have profound influence on the structures and performances of PVDF/HAPNPs blend membranes. The optimal membrane M2 with 2 wt% HAPNPs exhibited excellent hydrophilicity, outstanding mechanical strength of 19.60 MPa, and high water flux of (2466 +/- 31) Lm(-2)h(-1). The maximum static adsorption capacity of the optimal membrane was about 10.83 mg/g, which is 3.75 times that of the pristine PVDF membrane (2.89 mg/g). PVDF/HAPNPs membranes were not only utilized for static adsorption, but also applied to dynamic dye removal. The possible adsorption mechanism between Congo red (CR) and HAPNPs embedded on the blend membranes was firstly discussed in this work. HAPNPs interacted with CR via Lewis reaction, hydrogen bond interaction, as well as electrostatic attraction to achieve the adsorption effect. Herein, the PVDF/HAPNPs blend membranes with extraordinary hydrophilicity, mechanical strength, and dye removal efficiency possess tremendous potential for practical applications of wastewater treatment.
Keyword ：
Adsorption mechanism Adsorption mechanism Dynamic dye removal Dynamic dye removal HAPNPs blend membranes HAPNPs blend membranes Mechanical strength Mechanical strength PVDF PVDF Three dimensional hydroxyapatite nanoparticles Three dimensional hydroxyapatite nanoparticles
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| GB/T 7714 | Li, Jian-Hua , Zheng, Hui , Lin, Hua-Xiang et al. Preparation of Three Dimensional Hydroxyapatite Nanoparticles/Poly(vinylidene fluoride) Blend Membranes with Excellent Dye Removal Efficiency and Investigation of Adsorption Mechanism [J]. | CHINESE JOURNAL OF POLYMER SCIENCE , 2019 , 37 (12) : 1234-1247 . |
| MLA | Li, Jian-Hua et al. "Preparation of Three Dimensional Hydroxyapatite Nanoparticles/Poly(vinylidene fluoride) Blend Membranes with Excellent Dye Removal Efficiency and Investigation of Adsorption Mechanism" . | CHINESE JOURNAL OF POLYMER SCIENCE 37 . 12 (2019) : 1234-1247 . |
| APA | Li, Jian-Hua , Zheng, Hui , Lin, Hua-Xiang , Zhang, Bo-Xin , Wang, Jia-Bin , Li, Tong-Lei et al. Preparation of Three Dimensional Hydroxyapatite Nanoparticles/Poly(vinylidene fluoride) Blend Membranes with Excellent Dye Removal Efficiency and Investigation of Adsorption Mechanism . | CHINESE JOURNAL OF POLYMER SCIENCE , 2019 , 37 (12) , 1234-1247 . |
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A novel hydroxyapatite-embedded monolithic column has been facilely prepared in a stainless-steel column with inner diameter of 2.1 mm by the strong adhesion of urea-formaldehyde (UF) resin and exploited as a sorbent for selective on-line solid-phase extraction (on-line SPE) of adenosine triphosphate and its phosphorylated metabolites. The composition for this preparation, including the amount of hydroxyapatite nanopowders and the porogen were investigated to obtain a suitable monolith with large surface area and satisfactory permeability. Owing to anion exchange interaction of hydroxyapatite and hydrophilic interaction of UF monolithic matrix, the prepared monolith showed good extraction efficiency and selectivity towards these phosphorylated analytes. Several parameters for on-line SPE, including ACN percentage in the sampling solution, collection time span, salt concentration of the eluent, sampling and elution flow rate, were optimized with respect to the extraction efficiencies of the target compounds. Under the optimized conditions, the LODs of the analytes were in the range of 0.01-0.04 mu g/g, the recoveries in the spiked samples ranged from 78.3%-92.5% with RSDs < 4.7%. Due to the excellent extraction ability towards phosphorylated compounds in practical samples, a simple on-line SPE-HPLC method using hydroxyapatite-embedded monolith as sorbent has been proposed for monitoring freshness of grass carp.
Keyword ：
Adenosine triphosphate Adenosine triphosphate Hydroxyapatite Hydroxyapatite Monolithic column Monolithic column On-line solid-phase extraction On-line solid-phase extraction Phosphorylated metabolites Phosphorylated metabolites
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| GB/T 7714 | Wang, Jiabin , Li, Wenbang , Xiao, Jianhua et al. Hydroxyapatite-embedded monolithic column for selective on-line solid-phase extraction of adenosine triphosphate and its phosphorylated metabolites [J]. | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES , 2019 , 1128 . |
| MLA | Wang, Jiabin et al. "Hydroxyapatite-embedded monolithic column for selective on-line solid-phase extraction of adenosine triphosphate and its phosphorylated metabolites" . | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1128 (2019) . |
| APA | Wang, Jiabin , Li, Wenbang , Xiao, Jianhua , Ni, Bichen , Li, Jianhua , Wu, Jiulin et al. Hydroxyapatite-embedded monolithic column for selective on-line solid-phase extraction of adenosine triphosphate and its phosphorylated metabolites . | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES , 2019 , 1128 . |
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Based on our developed sodium hyaluronate-functionalized urea-formaldehyde (HA-UF) monolith, a silver nanoparticles-coated monolithic column has been fabricated via the interaction between silver nanoparticles (Ag NPs) and HA. The successful coating of Ag NPs on the parent monolith was proven by SEM, EDAX, UV-vis spectrum and XPS. Nitrogen adsorption desorption isotherms and Barret-JoynerHalenda (BJH) pore size distributions of the parent and resultant monolith were also performed. Due to the Ag+-like affinity interaction caused by the contact of unsaturated compounds and Ag NPs, the Ag NPs-coated monolith showed satisfactory extraction efficiency towards these compounds, and was applied for in-tube solid-phase microextraction (SPME) of monounsaturated fatty acid methyl esters (MUFAMEs). Several factors for in-tube SPME, such as ACN percentage in the sampling solution, elution volume, sampling and elution flow rate, were investigated with respect to the extraction efficiency of model MUFAMEs. Under the optimized SPME conditions, a simple in tube SPME-Ag+-HPLC method for detection of model MUFAMEs has been proposed, the limits of detection (LODs) were less than 5.2 mu g/kg, and the recoveries of spiked French fry samples were ranged from 86.6% to 96.1% with relative standard deviations (RSDs) less than 5.2%. This study provided an Ag NPs-coated monolith with good reproducibility and repeatable extraction performance, and developed an efficient method for in-tube SPME of MUFAMEs in practical food samples. (C) 2018 Elsevier B.V. All rights reserved.
Keyword ：
In-tube solid-phase microextraction In-tube solid-phase microextraction Monolithic column Monolithic column Monounsaturated fatty acid methyl esters Monounsaturated fatty acid methyl esters Silver nanoparticles Silver nanoparticles Trans fat acids Trans fat acids
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| GB/T 7714 | Jiang, Nan , Wang, Jiabin , Li, Wenbang et al. Silver nanoparticles-coated monolithic column for in-tube solid-phase microextraction of monounsaturated fatty acid methyl esters [J]. | JOURNAL OF CHROMATOGRAPHY A , 2019 , 1585 : 19-26 . |
| MLA | Jiang, Nan et al. "Silver nanoparticles-coated monolithic column for in-tube solid-phase microextraction of monounsaturated fatty acid methyl esters" . | JOURNAL OF CHROMATOGRAPHY A 1585 (2019) : 19-26 . |
| APA | Jiang, Nan , Wang, Jiabin , Li, Wenbang , Xiao, Jianhua , Li, Jianhua , Lin, Xucong et al. Silver nanoparticles-coated monolithic column for in-tube solid-phase microextraction of monounsaturated fatty acid methyl esters . | JOURNAL OF CHROMATOGRAPHY A , 2019 , 1585 , 19-26 . |
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A facile and versatile approach for the preparation of super-hydrophilic, excellent antifouling and hemocompatibility membranes had been developed through the generation in situ of bio-inspired poly-dopamine (PDA) microspheres on PVDF membranes. SEM images showed that the PDA microspheres were uniformly dispersed on the upper surface and the lower surface of the modified membranes. And there were a great number of PDA microspheres immobilized on the cross-section, but the interconnected pores structure was not destroyed. These facts indicated the existence of membrane micro-reactor effect for the whole membrane structure. Considering the remarkable improvement of hydrophilicity, antifouling properties, and permeation fluxes, we also proposed the cluster phenolic hydroxyl effect for the PVDF/PDA hybrid membranes. And the cluster phenolic hydroxyl effect can be ascribed to the all directions distributed phenolic hydroxyl groups on the whole membrane structure. Besides, the self-driven filtration experiments showed the great wetting ability and permeability of the PVDF/PDA hybrid membranes in filtration process without any external pressure. This implied the existence of accelerating self-driven force after the water flow flowed into the internal of membranes, which contributed to the increase of water flow velocity. All the three aspects were in favor of the enhancement of hydrophilicity, antifouling properties and permeability of the modified membranes. Moreover, the conventional filtration tests, oil/water emulsion filtration tests and protein adsorption tests were also carried out to discuss the practical applications of PVDF/PDA hybrid membranes. And the hemocompatibility of the modified membranes was also proved to enhance greatly through the hemolysis tests and platelet adhesion tests, indicating that the membranes were greatly promising in biomedical applications. The strategy of material modification reported here is substrate-independent and can be extended to other substrate materials, and allows the development of novel functional membranes through secondary treatments. (C) 2018 Elsevier B.V. All rights reserved.
Keyword ：
Hemocompatibility Hemocompatibility Membrane micro-reactor effect Membrane micro-reactor effect PVDF/PDA hybrid membranes PVDF/PDA hybrid membranes Self-driven Self-driven Super-hydrophilicity Super-hydrophilicity
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| GB/T 7714 | Li, Jian-Hua , Ni, Xing-Xing , Zhang, De-Bin et al. Engineering a self-driven PVDF/PDA hybrid membranes based on membrane micro-reactor effect to achieve super-hydrophilicity, excellent antifouling properties and hemocompatibility [J]. | APPLIED SURFACE SCIENCE , 2018 , 444 : 672-690 . |
| MLA | Li, Jian-Hua et al. "Engineering a self-driven PVDF/PDA hybrid membranes based on membrane micro-reactor effect to achieve super-hydrophilicity, excellent antifouling properties and hemocompatibility" . | APPLIED SURFACE SCIENCE 444 (2018) : 672-690 . |
| APA | Li, Jian-Hua , Ni, Xing-Xing , Zhang, De-Bin , Zheng, Hui , Wang, Jia-Bin , Zhang, Qi-Qing . Engineering a self-driven PVDF/PDA hybrid membranes based on membrane micro-reactor effect to achieve super-hydrophilicity, excellent antifouling properties and hemocompatibility . | APPLIED SURFACE SCIENCE , 2018 , 444 , 672-690 . |
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三磷酸腺苷 三磷酸腺苷 在线富集检测 在线富集检测 磷酸化代谢产物 磷酸化代谢产物 羟基磷灰石 羟基磷灰石 脲醛树脂整体柱 脲醛树脂整体柱
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| GB/T 7714 | 李文邦 , 王家斌 , 李建华 et al. 羟基磷灰石功能化整体柱的制备及其特异性富集性能研究 [C] //第十二届全国生物医药色谱及相关技术学术交流会论文集 . 2018 : 1-2 . |
| MLA | 李文邦 et al. "羟基磷灰石功能化整体柱的制备及其特异性富集性能研究" 第十二届全国生物医药色谱及相关技术学术交流会论文集 . (2018) : 1-2 . |
| APA | 李文邦 , 王家斌 , 李建华 , 张其清 . 羟基磷灰石功能化整体柱的制备及其特异性富集性能研究 第十二届全国生物医药色谱及相关技术学术交流会论文集 . (2018) : 1-2 . |
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