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学者姓名：陈立
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Abstract ：
Inspired by the formation of natural abalone shells (AS) similar to calcium salt deposition in human orthodontics, AS is used as an emulsifier in the scaffold to solve the problem of coexistence of natural and synthetic polymers and promote new bone formation. In this study, AS-stabilized and reinforced carboxymethyl chitosan/collagen/PLGA porous bionic composite scaffolds (AS/CMCS/Col/PLGA) were fabricated through the emulsion polymerization and bionic hybrid technology. As the addition of AS increased from 0.75 to 3.0 wt%, homogeneous distribution of flower-like particles could be observed on the inner surface of the scaffold, and its mechanical properties were improved. Particularly, 3.0 wt% AS-doped scaffolds (S3 and C + S3) exhibited excellent inorganic mineral deposition and osteoblast proliferation and differentiation abilities in vitro. In a SD rat calvarial defect model, they effectively promoted new bone formation in the defect and accelerated expression of osteogenic-angiogenic related proteins (COLI, OCN, VEGF). By virtue of its combined merits including good mechanical properties, inducing mineralization crystallization and facilitating osteogenesis, the 3.0 wt% AS-doped scaffold promises to be employed as a novel bone repair material for bone tissue regeneration. © 2024 Elsevier B.V.
Keyword ：
Abalone shell Abalone shell Bionic hybrid scaffold Bionic hybrid scaffold Carboxymethyl chitosan Carboxymethyl chitosan
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| GB/T 7714 | Pan, P. , Hu, Y. , Wang, C. et al. Abalone shells bioenhanced carboxymethyl chitosan/collagen/PLGA bionic hybrid scaffolds achieving biomineralization and osteogenesis for bone regeneration [J]. | International Journal of Biological Macromolecules , 2024 , 279 . |
| MLA | Pan, P. et al. "Abalone shells bioenhanced carboxymethyl chitosan/collagen/PLGA bionic hybrid scaffolds achieving biomineralization and osteogenesis for bone regeneration" . | International Journal of Biological Macromolecules 279 (2024) . |
| APA | Pan, P. , Hu, Y. , Wang, C. , Liu, Q. , Hu, L. , Yu, H. et al. Abalone shells bioenhanced carboxymethyl chitosan/collagen/PLGA bionic hybrid scaffolds achieving biomineralization and osteogenesis for bone regeneration . | International Journal of Biological Macromolecules , 2024 , 279 . |
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The formation of nacreous layers of abalone shell (Aba) is a similar process to the deposition of calcium salts in human bone, with the main mechanism being the orderly mineralization of inorganic matter mediated by organic bioactive components. In this study, 3D printing technology was employed to use the abundant calcium sources and bioactive substances in Aba to enhance the osteoinductive and remineralization capacity of materials in implants for bone regeneration. The novel hybrid scaffolds were successfully 3D-printed from polycaprolactone (PCL), which has good toughness and processability, doped with powdered Aba. When the Aba particle doping was increased from 0 to 15%, the physicochemical properties of PCL were virtually unchanged, while the surfaces of scaffolds became rough, and Aba particles were gradually exposed. The Aba/PCL scaffolds had an interconnected pore structure with a pore size of approximately 200 mu m and over 50% porosity, which was convenient for the transport of nutrients. With the addition of Aba, the thermodynamic stability and mechanical properties of the scaffolds significantly improved, and the maximum compressive strength and modulus reached 1.34 and 1.89 MPa, respectively, because Aba provided nucleation sites for nanohydroxyapatite (nHAP) to promote mineralization. In vitro cell experiments showed that the hybrid scaffolds had good biocompatibility and promote the proliferation of osteoblasts. In vivo results revealed that the hydroxyapatite of organic matter and trace elements in Aba particles induced the migration of stem cells and active factors to the site of a bone defect. The implantation of 3D-printed scaffolds offered a microenvironment for osteoblast attachment and proliferation, which further promotes osteogenesis-related gene expression, such as bone gamma carboxyglutamate protein (BGLAP), type I collagen (COL1A1), and secreted phosphoprotein 1 (SPP1), and facilitated the repair of a skull defect. This high-value applications of Aba have the potential to improve environmental pollution and provide potentially low-cost, high-performance bone repair materials for clinical use.
Keyword ：
3D printing scaffolds 3D printing scaffolds Bone regeneration Bone regeneration High-value utilization High-value utilization Micro-nano structure Micro-nano structure Nacreous layer of abalone shell Nacreous layer of abalone shell
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| GB/T 7714 | Pan, Panpan , Geng, Yusheng , Hu, Le et al. Biologically enhanced 3D printed micro-nano hybrid scaffolds doped with abalone shell for bone regeneration [J]. | ADVANCED COMPOSITES AND HYBRID MATERIALS , 2023 , 6 (1) . |
| MLA | Pan, Panpan et al. "Biologically enhanced 3D printed micro-nano hybrid scaffolds doped with abalone shell for bone regeneration" . | ADVANCED COMPOSITES AND HYBRID MATERIALS 6 . 1 (2023) . |
| APA | Pan, Panpan , Geng, Yusheng , Hu, Le , Liu, Qing , Liu, Man , Cheng, Meiqi et al. Biologically enhanced 3D printed micro-nano hybrid scaffolds doped with abalone shell for bone regeneration . | ADVANCED COMPOSITES AND HYBRID MATERIALS , 2023 , 6 (1) . |
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Marine shell resources have received great attention from researchers owing to their unique merits such as high hard-ness, good toughness, corrosion resistance, high adsorption, and bioactivity. Restricted by the level of comprehensive utilization technology, the utilization rate of shells is extremely low, resulting in serious waste and pollution. The research shows that the unique brick-mud structure of shells makes them have diverse and good functional character-istics, which guides them to have great utilization potential in different fields. Hence, this review highlights the con-stitutive relationship between microstructure-function-application of shells (e.g., gastropods, cephalopods, and amniotes), and the comprehensive applications and development ideas in the fields of biomedicine, adsorption enrich-ment, pHotocatalysis, marine carbon sink, and environmental deicer. It is worth mentioning that marine shells are cur-rently well developed in three areas: bone repair, health care and medicinal value, and drug carrier, which together promote the progress of biomedical field. In addition, an in-depth summary of the application of marine shells in the adsorption and purification of various impurities such as crude oil, heavy metal ions and dyes at low-cost and high efficiency is presented. Finally, by integrating thoughts and approaches from different applications, we are com-mitted to providing new pathways for the excavation and future high-value of shell resources, clarifying the existing development stages and bottlenecks, promoting the development of related technology industries, and achieving the synergistic win-win situation of economic and environmental benefits.
Keyword ：
Adsorption Adsorption Biomedicine Biomedicine High-value integrated utilization High-value integrated utilization Marine shell Marine shell Structure-effect relationship Structure-effect relationship
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| GB/T 7714 | Cheng, Meiqi , Liu, Man , Chang, Lirong et al. Overview of structure, function and integrated utilization of marine shell [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 870 . |
| MLA | Cheng, Meiqi et al. "Overview of structure, function and integrated utilization of marine shell" . | SCIENCE OF THE TOTAL ENVIRONMENT 870 (2023) . |
| APA | Cheng, Meiqi , Liu, Man , Chang, Lirong , Liu, Qing , Wang, Chunxiao , Hu, Le et al. Overview of structure, function and integrated utilization of marine shell . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 870 . |
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Most anticancer treatments only induce the death of ordinary cancer cells, while cancer stem cells (CSCs) in the quiescent phase of cell division are difficult to kill, which eventually leads to cancer drug resistance, metastasis, and relapse. Therefore, CSCs are also important in targeted cancer therapy. Herein, we developed dual-targeted and glutathione (GSH)-responsive novel nanoparticles (SSBPEI-DOX@siRNAs/iRGD-PEG-HA) to efficiently and specifically deliver both doxorubicin and small interfering RNA cocktails (siRNAs) (survivin siRNA, Bcl-2 siRNA and ABCG2 siRNA) to ovarian CSCs. They are fabricated via electrostatic assembly of anionic siRNAs and cationic disulfide bond crosslinking-branched polyethyleneimine-doxorubicin (SSBPEI-DOX) as a core. Interestingly, the SSBPEI-DOX could be degraded into low-cytotoxic polyethyleneimine (PEI). Because of the enrichment of glutathione reductase in the tumor microenvironment, the disulfide bond (-SS-) in SSBPEI-DOX can be specifically reduced to promote the controlled release of siRNA and doxorubicin (DOX) in the CSCs. siRNA cocktails could specifically silence three key genes in CSCs, which, in combination with the traditional chemotherapy drug DOX, induces apoptosis or necrosis of CSCs. iRGD peptides and "sheddable" hyaluronic acid (HA) wrapped around the core could mediate CSC targeting by binding with neuropilin-1 (NRP1) and CD44 to enhance delivery. In summary, the multifunctional delivery system SSBPEI-DOX@siRNAs/iRGD-PEG-HA nanoparticles displays excellent biocompatibility, accurate CSC-targeting ability, and powerful anti-CSC ability, which demonstrates its potential value in future treatments to overcome ovarian cancer metastasis and relapse. To support this work, as exhaustive search was conducted for the literature on nanoparticle drug delivery research conducted in the last 17 years (2007-2023) using PubMed, Web of Science, and Google Scholar.
Keyword ：
cancer stem cells cancer stem cells iRGD peptides iRGD peptides reduction sensitive reduction sensitive siRNAs siRNAs targeted delivery targeted delivery
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| GB/T 7714 | Chen, Li , Luo, Jinlan , Zhang, Jingyuan et al. Dual Targeted Nanoparticles for the Codelivery of Doxorubicin and siRNA Cocktails to Overcome Ovarian Cancer Stem Cells [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (14) . |
| MLA | Chen, Li et al. "Dual Targeted Nanoparticles for the Codelivery of Doxorubicin and siRNA Cocktails to Overcome Ovarian Cancer Stem Cells" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 14 (2023) . |
| APA | Chen, Li , Luo, Jinlan , Zhang, Jingyuan , Wang, Siyuan , Sun, Yang , Liu, Qinying et al. Dual Targeted Nanoparticles for the Codelivery of Doxorubicin and siRNA Cocktails to Overcome Ovarian Cancer Stem Cells . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (14) . |
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The clinical treatment of infected skin injuries caused by exogenous bacteria faces great challenges. Conventional therapeutic approaches are difficult to achieve synergistic effects of infection control and induction of skin regeneration. In this study, a novel tannic acid-based physically cross-linked double network hydrogel (PDH gel) was prepared on demand by covalent cross-linking of tannic acid (TA) with polyvinyl alcohol (PVA) and chelating ligand of TA with Fe3+. The homogeneity of the hydrogel was achieved by the action of glycol dispersant. With the anti-inflammatory and antioxidant properties of Fe3+ and TA, this hydrogel exhibited excellent antibacterial properties by achieving 99.69% and 99.36% bacterial inhibition against E.coli and S. aureus, respectively. Moreover, the PDH gel exhibits good biocompatibility, stretchability (up to 200%) and skin-friendliness. After 14 days of PDH-1 gel implantation in a rat model infected by S. aureus, the wound healing rate was as high as 95.21%. PDH gel-1 showed more granulation tissue, more pronounced blood vessels, higher collagen fiber density and good collagen deposition, and its recovery effect was better than that of PSH gel and PDH gel-2 in vivo. Hence, this study provides a novel avenue for the design of future clinical infected wound healing dressings.
Keyword ：
Double network Double network Fe 3+chelation Fe 3+chelation Homogeneous hydrogel Homogeneous hydrogel Infected wound healing Infected wound healing Tannic acid Tannic acid
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| GB/T 7714 | Cheng, Meiqi , Hu, Le , Xu, Gan et al. Tannic acid-based dual-network homogeneous hydrogel with antimicrobial and pro-healing properties for infected wound healing [J]. | COLLOIDS AND SURFACES B-BIOINTERFACES , 2023 , 227 . |
| MLA | Cheng, Meiqi et al. "Tannic acid-based dual-network homogeneous hydrogel with antimicrobial and pro-healing properties for infected wound healing" . | COLLOIDS AND SURFACES B-BIOINTERFACES 227 (2023) . |
| APA | Cheng, Meiqi , Hu, Le , Xu, Gan , Pan, Panpan , Liu, Qing , Zhang, Ziyue et al. Tannic acid-based dual-network homogeneous hydrogel with antimicrobial and pro-healing properties for infected wound healing . | COLLOIDS AND SURFACES B-BIOINTERFACES , 2023 , 227 . |
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To achieve smart and personalized medicine,the development of hydrogel dressings with sensing proper-ties and biotherapeutic properties that can act as a sensor to monitor of human health in real-time while speeding up wound healing face great challenge.In the present study,a biocompatible dual-network composite hydrogel(DNCGel)sensor was obtained via a simple process.The dual network hydrogel is constructed by the interpenetration of a flexible network formed of poly(vinyl alcohol)(PVA)physical cross-linked by repeated freeze-thawing and a rigid network of iron-chelated xanthan gum(XG)impreg-nated with Fe3+interpenetration.The pure PVA/XG hydrogels were chelated with ferric ions by immer-sion to improve the gel strength(compressive modulus and tensile modulus can reach up to 0.62 MPa and 0.079 MPa,respectively),conductivity(conductivity values ranging from 9 × 10-4 S/cm to 1 × 10-3 S/cm)and bacterial inhibition properties(up to 98.56％).Subsequently,the effects of the ratio of PVA and XG and the immersion time of Fe3+on the hydrogels were investigated,and DNGel3 was given the most priority on a comprehensive consideration.It was demonstrated that the DNCGel exhibit good biocom-patibility in vitro,effectively facilitate wound healing in vivo(up to 97.8％healing rate)under electrical stimulation,and monitors human movement in real time.This work provides a novel avenue to explore multifunctional intelligent hydrogels that hold great promise in biomedical fields such as smart wound dressings and flexible wearable sensors.
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| GB/T 7714 | Le Hu , Yuxin Wang , Qing Liu et al. Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing [J]. | 中国化学快报（英文版） , 2023 , 34 (10) : 229-235 . |
| MLA | Le Hu et al. "Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing" . | 中国化学快报（英文版） 34 . 10 (2023) : 229-235 . |
| APA | Le Hu , Yuxin Wang , Qing Liu , Man Liu , Faming Yang , Chunxiao Wang et al. Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing . | 中国化学快报（英文版） , 2023 , 34 (10) , 229-235 . |
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样品前处理是色谱分析中必不可少的环节。固相萃取是一类应用广泛的前处理方法，吸附剂的优劣直接影响萃取过程对目标化合物的吸附和富集效率，并影响前处理及后续分析方法的灵敏度和选择性。因此吸附剂的选择和开发成为一个研究热点。微孔有机网络（microporous organic networks,MONs）是由芳香炔烃和芳香卤化物通过Sonogashira反应合成的一类新型共价有机材料，具有结构可修饰、比表面积大、孔隙率高、合成简单等优点。本文概述了MONs的合成和功能化修饰方法，着重介绍了该材料在样品前处理领域的应用新进展，并对其发展趋势进行了展望。在合成方法方面，MONs材料的制备从回流合成法、溶剂热合成法发展到室温合成法，合成条件趋向于更温和、更高效。在材料功能化修饰方面，引入大分子物质以及氨基、羟基、羧基等活性基团，能增加MONs材料的选择性和作用位点；将MONs与Fe_3O_4、SiO_2、MOFs结合，形成核壳结构MONs，在此基础上进行煅烧和刻蚀，可形成多孔碳结构或空心多层材料。上述功能化修饰的MONs及其复合材料和目标物之间存在多重作用机制（氢键、疏水、静电、π-π相互作用等），因此能实现各类化合物的高效萃取。将MONs作为吸附剂材料应用于固相萃取、固相微萃取、分散固相萃取、磁性固相萃取等多种前处理方法，结合色谱、色谱-质谱联用等技术，获得了较好的吸附效果和较高的灵敏度，展现了MONs材料在样品前处理领域的应用潜力。
Keyword ：
Sonogashira反应 Sonogashira反应 吸附剂 吸附剂 固相萃取 固相萃取 微孔有机网络 微孔有机网络 样品前处理 样品前处理 综述 综述
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| GB/T 7714 | 余涛 , 陈立 , 张文敏 et al. 微孔有机网络材料的合成方法及其在样品前处理中的应用进展 [J]. | 色谱 , 2023 , 41 (12) : 1052-1061 . |
| MLA | 余涛 et al. "微孔有机网络材料的合成方法及其在样品前处理中的应用进展" . | 色谱 41 . 12 (2023) : 1052-1061 . |
| APA | 余涛 , 陈立 , 张文敏 , 张兰 , 卢巧梅 . 微孔有机网络材料的合成方法及其在样品前处理中的应用进展 . | 色谱 , 2023 , 41 (12) , 1052-1061 . |
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To achieve smart and personalized medicine, the development of hydrogel dressings with sensing proper-ties and biotherapeutic properties that can act as a sensor to monitor of human health in real-time while speeding up wound healing face great challenge. In the present study, a biocompatible dual-network composite hydrogel (DNCGel) sensor was obtained via a simple process. The dual network hydrogel is constructed by the interpenetration of a flexible network formed of poly(vinyl alcohol) (PVA) physical cross-linked by repeated freeze-thawing and a rigid network of iron-chelated xanthan gum (XG) impreg-nated with Fe3+ interpenetration. The pure PVA/XG hydrogels were chelated with ferric ions by immer-sion to improve the gel strength (compressive modulus and tensile modulus can reach up to 0.62 MPa and 0.079 MPa, respectively), conductivity (conductivity values ranging from 9 x 10 -4 S/cm to 1 x 10 -3 S/cm) and bacterial inhibition properties (up to 98.56%). Subsequently, the effects of the ratio of PVA and XG and the immersion time of Fe3+ on the hydrogels were investigated, and DNGel3 was given the most priority on a comprehensive consideration. It was demonstrated that the DNCGel exhibit good biocom-patibility in vitro , effectively facilitate wound healing in vivo (up to 97.8% healing rate) under electrical stimulation, and monitors human movement in real time. This work provides a novel avenue to explore multifunctional intelligent hydrogels that hold great promise in biomedical fields such as smart wound dressings and flexible wearable sensors.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
Keyword ：
Antimicrobial activity Antimicrobial activity Conductive hydrogel Conductive hydrogel Dual cross-linked network Dual cross-linked network Real-time monitorin Real-time monitorin Wound healing Wound healing
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| GB/T 7714 | Hu, Le , Wang, Yuxin , Liu, Qing et al. Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing [J]. | CHINESE CHEMICAL LETTERS , 2023 , 34 (10) . |
| MLA | Hu, Le et al. "Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing" . | CHINESE CHEMICAL LETTERS 34 . 10 (2023) . |
| APA | Hu, Le , Wang, Yuxin , Liu, Qing , Liu, Man , Yang, Faming , Wang, Chunxiao et al. Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing . | CHINESE CHEMICAL LETTERS , 2023 , 34 (10) . |
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The polycaprolactone (PCL) and nano-hydroxyapatite (nHAP) composite is an attractive material for bone scaffolds with excellent mechanical properties and osteoinductivity. It also exhibited good biocompatibility as well as controllable biodegradability. We have recently developed a PCL and nHAP composite scaffold, which is embedded with poly (lactic-co-glycolic acid) (PLGA) microspheres (mu s). It achieved controlled delivery of bioactive factors. In this study, Icariin (ICA) encapsulated with PLGA mu s was embedded in 3D printed PCL/nHAP scaffolds to facilitate in situ bone regeneration. The scaffold exhibited excellent mechanical performance owing to the nHAP. The PCL/nHAP scaffold showed sustainable release of ICA as the PCL degraded. The PCL degradation produced cracks on the surface of the scaffold, and then the PLGA mu s was exposed to phosphate buffer solution. The released ICA promoted the osteogenic differentiation of MC3T3-E1. Consistently, in vivo studies showed that the composite scaffolds releasing ICA promoted the healing of calvaria bone. In conclusion, PCL/ PLGA mu s/nHAP composite scaffold by 3D printing may serve as an efficient material for bone tissue repair and regeneration.
Keyword ：
3D printing 3D printing Biodegradability Biodegradability Bone regeneration Bone regeneration Controlled release Controlled release Facilitated endogenous tissue engineering Facilitated endogenous tissue engineering
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| GB/T 7714 | Zou, Lin , Hu, Le , Pan, Panpan et al. Icariin-releasing 3D printed scaffold for bone regeneration [J]. | COMPOSITES PART B-ENGINEERING , 2022 , 232 . |
| MLA | Zou, Lin et al. "Icariin-releasing 3D printed scaffold for bone regeneration" . | COMPOSITES PART B-ENGINEERING 232 (2022) . |
| APA | Zou, Lin , Hu, Le , Pan, Panpan , Tarafder, Solaiman , Du, Mingzu , Geng, Yusheng et al. Icariin-releasing 3D printed scaffold for bone regeneration . | COMPOSITES PART B-ENGINEERING , 2022 , 232 . |
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As a mussel-inspired polymer, polydopamine (PDA) coating can be used as a simple and universal way to form nano-hydroxyapatite (nHAP) crystals on a variety of substrates. Therefore, PDA is widely used in the preparation of bone repair materials. However, its uneven distribution and easy degradation under strongly alkaline con-ditions limit its application. Therefore, based on the principle of in situ regeneration, this study combined the freeze-drying technology with the diffusion method of ammonium carbonate to bionics construct porous micro -nano composite scaffolds. Chitosan (CS) and dopamine hydrochloride (DA) were used as the chief components to form a multilayer layered scaffold structure. PDA and nHAP grow in situ on the surface of the freeze-dried scaffolds after ammonium carbonate gas diffusion treatment and Tris buffer immersion. The mild synthesis method effectively avoided the decomposition of PDA, and the gas diffusion method promoted the uniform distribution of PDA. The effect of PDA on nHAP formation was studied by adjusting the amount of DA. The cross -linking reaction between PDA and CS was verified by FTIR and XPS. Surface morphology was observed by SEM. The formation of nHAP was confirmed by XRD. The scaffolds had good porosity, degradation, water absorption, thermal stability and biomimetic mineralization properties. Cell experiments showed that the scaffolds had good biocompatibility and had no toxicity to cells. The construction of bone defect animal model further verified that the scaffolds promoted bone regeneration in the defect site of the body. This study provided a new idea and method for the preparation of bone repair scaffolds.
Keyword ：
Bone regeneration Bone regeneration Gas diffusion method Gas diffusion method In situ mineralization In situ mineralization Micro-nano hybrid scaffolds Micro-nano hybrid scaffolds Mussel-inspired polymerization Mussel-inspired polymerization
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| GB/T 7714 | Yang, Yifan , Geng, Yusheng , Liu, Min et al. Facile mussel-inspired polymerization to facilitate biomimetic in situ homogeneous mineralization for bone regeneration [J]. | COMPOSITES PART B-ENGINEERING , 2022 , 247 . |
| MLA | Yang, Yifan et al. "Facile mussel-inspired polymerization to facilitate biomimetic in situ homogeneous mineralization for bone regeneration" . | COMPOSITES PART B-ENGINEERING 247 (2022) . |
| APA | Yang, Yifan , Geng, Yusheng , Liu, Min , Liu, Kaihua , Lv, Xiaoyu , Yu, Hui et al. Facile mussel-inspired polymerization to facilitate biomimetic in situ homogeneous mineralization for bone regeneration . | COMPOSITES PART B-ENGINEERING , 2022 , 247 . |
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