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学者姓名:刘志彬

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Theoretical insight into the antioxidant activity of Theasinensin A SCIE
期刊论文 | 2025 , 477 | FOOD CHEMISTRY
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Abstract :

Theasinensin A (TSA), a dimeric flavan-3-ol from fermented teas, exhibits potent antioxidant activity exceeding that of its monomeric precursor, epigallocatechin gallate (EGCG). This study integrates experimental and computational methods (Density Functional Theory) to elucidate TSA's antioxidant mechanisms. DPPH assays revealed that TSA exhibited an IC50 value 2.4-fold lower than that of EGCG, indicating its superior radical scavenging efficacy. Electrostatic potential, frontier molecular orbital, and Laplacian bond order analyses identified the O4' - H group on the B-ring as the primary reaction site, with TSA showing higher reactivity than EGCG. Reaction enthalpy calculations further revealed that TSA requires less energy for double hydrogen donation, underscoring its thermodynamic advantage. Additionally, spin density analysis demonstrated the increased stability of TSA's phenoxy radical, which may be stabilized by its robust intramolecular hydrogen bonding, it-it stacking, and CH-it interactions. These findings highlight the unique structural and electronic properties of TSA that contribute to its superior antioxidant efficacy.

Keyword :

Antioxidant activity Antioxidant activity DFT DFT Non-covalent interaction Non-covalent interaction Reaction enthalpy Reaction enthalpy Spin density Spin density Theasinensin A Theasinensin A

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GB/T 7714 Chen, Yizhe , Sheng, Liping , Ni, Li et al. Theoretical insight into the antioxidant activity of Theasinensin A [J]. | FOOD CHEMISTRY , 2025 , 477 .
MLA Chen, Yizhe et al. "Theoretical insight into the antioxidant activity of Theasinensin A" . | FOOD CHEMISTRY 477 (2025) .
APA Chen, Yizhe , Sheng, Liping , Ni, Li , Feng, Xiaoxiao , Liu, Yuan , Liu, Zhibin . Theoretical insight into the antioxidant activity of Theasinensin A . | FOOD CHEMISTRY , 2025 , 477 .
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Insight into the mechanism of roasting-induced characteristic aroma formation in Wuyi rock tea using an "in-leaf" model with isotopic labeling SCIE
期刊论文 | 2025 , 474 | FOOD CHEMISTRY
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Abstract :

Wuyi Rock Tea (WRT) undergoes complex chemical transformations during roasting that significantly influence its aroma. This study explores the mechanisms involved in the formation of key aroma compounds, specifically alkylpyrazines and furans. Using HS-SPME-GC-O-MS and aroma dilution analysis, we identified 15 odor-active compounds, including three alkylpyrazines (2,6-diethyl-pyrazine, 2-ethyl-3,5-dimethyl-pyrazine, and 2-methyl3,5-diethyl-pyrazine) and two furans (furfural and 5-methyl-2-furancarboxaldehyde), with odor-active values exceeding 1. An "in-leaf" labeling approach utilizing isotopically labeled alanine ([15N]-Ala) and glucose ([U-13C6]-Glc) was employed to explore the contributions of tea leaf matrix and soluble compounds. Results indicated that the incorporation of two nitrogen atoms from alanine was the predominant pathway for alkylpyrazine formation, while five or six carbon atoms from glucose were primarily responsible for furan formation. Additionally, the insoluble tea leaf matrix contributed 13 % to 32 % of alkylpyrazines and 12 % to 25 % of furans. These findings enhance our understanding of the Maillard reaction's role in WRT's flavor development.

Keyword :

Alkylpyrazines Alkylpyrazines Furans Furans "In-leaf" model "In-leaf" model Roasting processing Roasting processing Stable isotope-labeling Stable isotope-labeling Wuyi rock tea Wuyi rock tea

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GB/T 7714 Chen, Wensong , Liu, Wangxin , Liu, Zhibin et al. Insight into the mechanism of roasting-induced characteristic aroma formation in Wuyi rock tea using an "in-leaf" model with isotopic labeling [J]. | FOOD CHEMISTRY , 2025 , 474 .
MLA Chen, Wensong et al. "Insight into the mechanism of roasting-induced characteristic aroma formation in Wuyi rock tea using an "in-leaf" model with isotopic labeling" . | FOOD CHEMISTRY 474 (2025) .
APA Chen, Wensong , Liu, Wangxin , Liu, Zhibin , Wang, Daoliang , Lan, Xiaoye , Zhan, Sijia et al. Insight into the mechanism of roasting-induced characteristic aroma formation in Wuyi rock tea using an "in-leaf" model with isotopic labeling . | FOOD CHEMISTRY , 2025 , 474 .
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A review of umami taste of Tea: Substances, perception mechanism, and physiological measurement prospects EI
期刊论文 | 2025 , 162 | Trends in Food Science and Technology
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Background: Tea, celebrated for its delightful flavors and health benefits, is a globally cherished beverage. Umami taste is an essential contributor to its flavor profile and its perception mechanism is crucial for enhancing the taste quality of tea. The exploration of individual physiological and emotional responses to gustatory signals through physiological measurement techniques makes it possible to decode the mechanisms underlying umami perception. Scope and approach: This article focuses on the umami taste of tea, providing a comprehensive review of the umami substances and the influencing factors. It discusses the umami perception mechanism from the perspectives of both umami-contributing and umami-enhancing aspects. Additionally, the paper explores the application prospects of physiological measurement technologies in decoding umami perception. This review aims to provide new analytical strategies for decoding umami and other taste perceptions. Key findings and conclusions: Umami is one of the important sensory attributes contributing to the taste of tea products. The umami taste in tea is predominantly mediated by amino acids such as L-theanine, L-glutamic acid, and L-aspartic acid, with additional contributions from compounds like succinic acid and methylated catechins. The interaction of these umami compounds with the T1R1/T1R3 receptor complex involves a suite of molecular forces, including hydrogen bonding, hydrophobic interactions, van der Waals forces, and electrostatic interactions, which are essential for the stability and functionality of ligand-receptor binding. Neuroimaging and electrophysiological studies have further elucidated the neural basis of umami perception, providing a comprehensive understanding of the sensory input to cognitive processing associated with this taste modality. © 2025 Elsevier Ltd

Keyword :

Brain Brain Brain mapping Brain mapping Cartilage Cartilage Hydrogen bonds Hydrogen bonds Neurons Neurons Physiological models Physiological models Sensory perception Sensory perception

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GB/T 7714 Yu, Yashu , Feng, Xiaoxiao , Blank, Imre et al. A review of umami taste of Tea: Substances, perception mechanism, and physiological measurement prospects [J]. | Trends in Food Science and Technology , 2025 , 162 .
MLA Yu, Yashu et al. "A review of umami taste of Tea: Substances, perception mechanism, and physiological measurement prospects" . | Trends in Food Science and Technology 162 (2025) .
APA Yu, Yashu , Feng, Xiaoxiao , Blank, Imre , Wang, Haoli , Zhu, Yiwen , Liu, Zhibin et al. A review of umami taste of Tea: Substances, perception mechanism, and physiological measurement prospects . | Trends in Food Science and Technology , 2025 , 162 .
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Sensory and molecular insights into the bitterness of Wuyi rock tea SCIE
期刊论文 | 2024 , 59 | FOOD BIOSCIENCE
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Wuyi Rock Tea (WRT) is widely appreciated for its unique rocky flavor and floral essence. However, the characterization of taste attributes, particularly bitterness, in this premium tea remains largely underexplored. This study aims to assess the perception of bitterness in WRT through an integrated approach, including sensory evaluation, phenolic compound analysis, and cell-based calcium imaging assay. Sensory evaluation revealed distinct bitterness intensities among nine selected typical WRT samples. Utilizing UHPLC-Q-Orbitrap-MS, 54 phenolics were tentatively identified, comprising of 24 flavanols, 18 flavonols, and 12 phenolic acids. The cellbased calcium imaging assay revealed diverse intracellular calcium responses and half-maximum effective concentration (EC50) values across the tested samples. Notably, correlation analysis unveiled a strong negative correlation between sensory scores and EC50 values. Furthermore, 11 individual phenolic compounds, such as epigallocatechin gallate and epicatechin gallate, demonstrated significant correlation with sensory scores and bitter taste receptor activation, suggesting their potential role as key contributors shaping the bitter taste perception of WRT. This study provides comprehensive insights into the nuanced bitterness of WRT, bridging sensory and molecular perspectives to enhance our understanding of the taste profile of this premium tea.

Keyword :

Bitterness Bitterness Cell-based calcium imaging Cell-based calcium imaging Phenolic compound Phenolic compound Sensory evaluation Sensory evaluation UHPLC-Q-orbitrap-MS UHPLC-Q-orbitrap-MS Wuyi rock tea Wuyi rock tea

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GB/T 7714 Lu, Yuan , Sun, Yaqian , Ni, Li et al. Sensory and molecular insights into the bitterness of Wuyi rock tea [J]. | FOOD BIOSCIENCE , 2024 , 59 .
MLA Lu, Yuan et al. "Sensory and molecular insights into the bitterness of Wuyi rock tea" . | FOOD BIOSCIENCE 59 (2024) .
APA Lu, Yuan , Sun, Yaqian , Ni, Li , Su, Weiying , Liu, Yuan , Feng, Xiaoxiao et al. Sensory and molecular insights into the bitterness of Wuyi rock tea . | FOOD BIOSCIENCE , 2024 , 59 .
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Insight into aroma dynamic changes during the whole manufacturing process of smoked Lapsang Souchong tea SCIE
期刊论文 | 2024 , 463 | FOOD CHEMISTRY
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Smoked Lapsang Souchong tea (SLST) is a distinctive black tea known for its smokey, fruity, and pine flavor. This study analyzed the aroma dynamic changes during the whole manufacturing process of SLST utilizing E-nose, HSSPME-GC-MS, and HS-SPME-GC-O-MS. Fresh tea leaves primarily contain green and floral volatiles, such as (E)2-hexenal (OAV 33.41) and linalool (OAV 313.88). Withering and drying processes introduce pinewood smokederived phenolic and terpenoid compounds, such as guaiacols (OAV 77.05) and alpha-terpineol (OAV 1.08), crucial for the tea's smoky and woody aroma. The study further highlights glycoside hydrolysis and lipid oxidation pathways in generating key tea-derived volatiles during fermentation, contributing to the fruity and floral notes in SLST. Pinewood smoke was identified as a major source of smoky volatiles, with longifolene and alpha-terpineol being unique to pinewood. These findings elucidate the formation pathways of the key volatile compounds and the impact of traditional processing on SLST's aroma profile.

Keyword :

Aroma dynamics Aroma dynamics GC-O-MS GC-O-MS Manufacturing process Manufacturing process Pinewood Pinewood Smoked Lapsang Souchong tea Smoked Lapsang Souchong tea Volatile compounds Volatile compounds

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GB/T 7714 Su, Weiying , Liu, Zhibin , Huang, Simin et al. Insight into aroma dynamic changes during the whole manufacturing process of smoked Lapsang Souchong tea [J]. | FOOD CHEMISTRY , 2024 , 463 .
MLA Su, Weiying et al. "Insight into aroma dynamic changes during the whole manufacturing process of smoked Lapsang Souchong tea" . | FOOD CHEMISTRY 463 (2024) .
APA Su, Weiying , Liu, Zhibin , Huang, Simin , Wang, Daoliang , Feng, Xiaoxiao , Liu, Yuan et al. Insight into aroma dynamic changes during the whole manufacturing process of smoked Lapsang Souchong tea . | FOOD CHEMISTRY , 2024 , 463 .
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New Insights into the Umami and Sweet Taste of Oolong Tea: Formation of Enhancer N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (Alapyridaine) in Roasting Via Maillard Reaction SCIE
期刊论文 | 2024 , 72 (15) , 8760-8773 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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Roasting is pivotal for enhancing the flavor of Wuyi rock tea (WRT). A study investigated a novel compound that enhances the umami taste of WRT. Metabolomics of Shuixian tea (SXT) and Rougui tea (RGT) under light roasting (LR), medium roasting (MR), and heavy roasting (HR) revealed significant differences in nonvolatiles compounds. Compared LR reducing sugars and amino acids notably decreased in MR and HR, with l-alanine declining by 69%. Taste-guided fractionation identified fraction II-B as having high umami and sweet intensities. A surprising taste enhancer, N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (alapyridaine), was discovered and identified. It formed via the Maillard reaction, positively correlated with roasting in SXT and RGT. Alapyridaine levels were highest in SXT among the five oolong teas. Roasting tea with glucose increased alapyridaine levels, while EGCG inhibited its formation. HR-WRT exhibited enhanced umami and sweet taste, highlighting alapyridaine's impact on WRT's flavor profile. The formation of alapyridaine during the roasting process provides new insights into the umami and sweet perception of oolong tea.

Keyword :

Maillard reaction Maillard reaction N-(1-carboxyethyl)-6-(hydroxymethyl)pyridinium-3-ol N-(1-carboxyethyl)-6-(hydroxymethyl)pyridinium-3-ol Roastedtea Roastedtea Sweet Sweet Umami Umami Wuyi Rock tea Wuyi Rock tea

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GB/T 7714 Feng, Xiaoxiao , Wang, Haoli , Zhu, Yiwen et al. New Insights into the Umami and Sweet Taste of Oolong Tea: Formation of Enhancer N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (Alapyridaine) in Roasting Via Maillard Reaction [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (15) : 8760-8773 .
MLA Feng, Xiaoxiao et al. "New Insights into the Umami and Sweet Taste of Oolong Tea: Formation of Enhancer N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (Alapyridaine) in Roasting Via Maillard Reaction" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 15 (2024) : 8760-8773 .
APA Feng, Xiaoxiao , Wang, Haoli , Zhu, Yiwen , Ma, Jingke , Ke, Yu , Wang, Kequn et al. New Insights into the Umami and Sweet Taste of Oolong Tea: Formation of Enhancer N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (Alapyridaine) in Roasting Via Maillard Reaction . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (15) , 8760-8773 .
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Differences of Typical Wuyi Rock Tea in Taste and Nonvolatiles Profile Revealed by Multisensory Analysis and LC-MS-Based Metabolomics SCIE
期刊论文 | 2024 , 72 (15) , 8715-8730 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
WoS CC Cited Count: 6
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Wuyi Rock tea, specifically Shuixian and Rougui, exhibits distinct sensory characteristics. In this study, we investigated the sensory and metabolite differences between Shuixian and Rougui. Quantitative description analysis revealed that Rougui exhibited higher intensity in bitter, thick, harsh, and numb tastes, while Shuixian had stronger salty and umami tastes. Nontargeted metabolomics identified 151 compounds with 66 compounds identified as key differential metabolites responsible for metabolic discrimination. Most of the catechins and flavonoids were enriched in Rougui tea, while epigallocatechin-3,3 '-di-O-gallate, epigallocatechin-3,5-di-O-gallate, gallocatechin-3,5-di-O-gallate, isovitexin, and theaflavanoside I were enriched in Shuixian tea. Catechins, kaempferol, quercetin, and myricetin derivatives were positively correlated with bitter taste and numb sensation. Sour taste was positively correlated to organic acids. Amino acids potentially contributed to salty and umami tastes. These results provide further insights into the taste characteristics and the relationship between taste attributes and specific metabolites in Wuyi Rock tea.

Keyword :

amino acid amino acid catechin catechin metabolomics metabolomics oolong tea oolong tea taste characteristics taste characteristics

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GB/T 7714 Wang, Haoli , Feng, Xiaoxiao , Blank, Imre et al. Differences of Typical Wuyi Rock Tea in Taste and Nonvolatiles Profile Revealed by Multisensory Analysis and LC-MS-Based Metabolomics [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (15) : 8715-8730 .
MLA Wang, Haoli et al. "Differences of Typical Wuyi Rock Tea in Taste and Nonvolatiles Profile Revealed by Multisensory Analysis and LC-MS-Based Metabolomics" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 15 (2024) : 8715-8730 .
APA Wang, Haoli , Feng, Xiaoxiao , Blank, Imre , Zhu, Yiwen , Liu, Zhibin , Ni, Li et al. Differences of Typical Wuyi Rock Tea in Taste and Nonvolatiles Profile Revealed by Multisensory Analysis and LC-MS-Based Metabolomics . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (15) , 8715-8730 .
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Exploration of the flavor diversity of oolong teas: A comprehensive analysis using metabolomics, quantification techniques, and sensory evaluation SCIE
期刊论文 | 2024 , 195 | FOOD RESEARCH INTERNATIONAL
WoS CC Cited Count: 5
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The taste profile of oolong tea is intricately shaped by origins and roasting. Herein, a comprehensive approach integrating non-targeted metabolomics, quantitative analysis and sensory evaluations was employed to analyze the taste profile of oolong tea. 25 selected representative oolong teas, including Southern Fujian (MN), Northern Fujian (MB), and Taiwan (TW), were meticulously were classified into SX-RG-DD, GS, and TGY based on the chemical taste phenotypes. A total of 314 non-volatile compounds were identified, among which 87 and 77 compounds, including catechin, theaflavins, flavonoids and amino acids were screened as critical taste metabolites responsible for regions and roasting degree, respectively. The reduction of bitter and astringent, coupled with the enhancement of umami, sweet and sweet aftertaste exhibited a correlation with the decrease in (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG), while the increase in catechin (C). These findings provide insights for further research on optimizing tea quality and refining processing techniques.

Keyword :

Catechin Catechin Metabolomics Metabolomics Oolong tea Oolong tea Region Region Roasting Roasting Taste Taste

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GB/T 7714 Feng, Xiaoxiao , Wang, Haoli , Yu, Yashu et al. Exploration of the flavor diversity of oolong teas: A comprehensive analysis using metabolomics, quantification techniques, and sensory evaluation [J]. | FOOD RESEARCH INTERNATIONAL , 2024 , 195 .
MLA Feng, Xiaoxiao et al. "Exploration of the flavor diversity of oolong teas: A comprehensive analysis using metabolomics, quantification techniques, and sensory evaluation" . | FOOD RESEARCH INTERNATIONAL 195 (2024) .
APA Feng, Xiaoxiao , Wang, Haoli , Yu, Yashu , Zhu, Yiwen , Ma, Jingke , Liu, Zhibin et al. Exploration of the flavor diversity of oolong teas: A comprehensive analysis using metabolomics, quantification techniques, and sensory evaluation . | FOOD RESEARCH INTERNATIONAL , 2024 , 195 .
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Unveiling the aromatic intricacies of Wuyi Rock Tea: A comparative study on sensory attributes and odor-active compounds of Rougui and Shuixian varieties SCIE
期刊论文 | 2023 , 435 | FOOD CHEMISTRY
WoS CC Cited Count: 4
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The distinctive fragrance of Wuyi Rock Tea (WRT) has garnered high attention in recent years. Herein, we conducted a comprehensive comparison of the sensory attributes and odor-active compounds (OACs) between two quintessential WRTs, namely Rougui (RGT) and Shuixian (SXT). Sensory analysis revealed that RGT exhibited a more pronounced fruity aroma, while SXT had a more complex and intricate sensory profile. By using gas chromatography-olfactory mass spectrometry (GC-O-MS) and two-dimensional gas chromatography time-of-flight mass spectrometry (GC x GC-TOF-MS) analyses, 26 OACs were identified. Among them, 12 compounds with odor activity values > 1 were recognized as key OACs. Noteworthily, eight compounds, including 6-methyl-5-hepten-2-one, 2-ethyl-3,5-dimethylpyrazine, 2,3-diethyl-5-methylpyrazine, linalool, methyl salicylate, gera-niol, (E)-8-ionone, and (E)-nerolidol, were shared by both teas. The unique compounds for RGT were (E)-linalool oxide and (Z)-jasmone, while those for SXT were 8-cyclocitral and alpha-ionone. These findings offer valuable in-sights for better understanding the flavor differences between the two most important types of WRT.

Keyword :

etry (GC-O-MS) etry (GC-O-MS) flight mass spectrometry (GC xGC-TOF-MS) flight mass spectrometry (GC xGC-TOF-MS) Gas chromatography-olfactory mass spectrom Gas chromatography-olfactory mass spectrom Key odor-active compounds Key odor-active compounds Sensomics Sensomics Two-dimensional gas chromatography time-of Two-dimensional gas chromatography time-of Wuyi Rock Tea Wuyi Rock Tea

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GB/T 7714 Wang, Daoliang , Liu, Zhibin , Lan, Xiaoye et al. Unveiling the aromatic intricacies of Wuyi Rock Tea: A comparative study on sensory attributes and odor-active compounds of Rougui and Shuixian varieties [J]. | FOOD CHEMISTRY , 2023 , 435 .
MLA Wang, Daoliang et al. "Unveiling the aromatic intricacies of Wuyi Rock Tea: A comparative study on sensory attributes and odor-active compounds of Rougui and Shuixian varieties" . | FOOD CHEMISTRY 435 (2023) .
APA Wang, Daoliang , Liu, Zhibin , Lan, Xiaoye , Wang, Cainan , Chen, Wensong , Zhan, Sijia et al. Unveiling the aromatic intricacies of Wuyi Rock Tea: A comparative study on sensory attributes and odor-active compounds of Rougui and Shuixian varieties . | FOOD CHEMISTRY , 2023 , 435 .
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Role of roasting in the formation of characteristic aroma of wuyi rock tea SCIE
期刊论文 | 2023 , 147 | FOOD CONTROL
WoS CC Cited Count: 19
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Roasting is one of the most critical manufacturing steps for making Wuyi Rock tea (WRT). The role of roasting in the formation of characteristic aroma of WRT was elucidated in detail in this study. First, the volatiles formed during the roasting process of WRT were investigated by using HS-SPME-GC-MS. Next, 18 volatiles were iden-tified as odor active compounds (flavor dilution factor >= 1) and 5 volatiles were identified as key aromatic compounds (odor activity value >= 1) by using HS-SPME-GC-O-MS and aroma recombination tests. Furthermore, model reactions of fructose, amino acids, and epicatechin, mimicking the Maillard reaction occurred during roasting, indicated that roasting temperature, amino acid type, and catechins were critical factors for the for-mation of the characteristic aroma of WRT. In specific, the temperature above 120 degrees C facilitated the formation of 2,3-diethyl-5-methylpyrazine, 5-methyl-2-furancarboxaldehyde and 2,6-diethyl pyrazine. And high temperature roasting also facilitated the elimination of off-flavors with grassy odor formed during tea storage. The model reactions of fructose with alanine and lysine at 130 degrees C formed the highest abundance of characteristic aroma of WRT. Additionally, it was also found that epicatechin inhibited the formation of the characteristic aromatic compounds of WRT. Taken together, this study provides details regarding the dynamics of volatiles during roasting, and elucidates the roles of roasting on the formation of the characteristic aroma of WRT.

Keyword :

Formation mechanism Formation mechanism Maillard reaction model Maillard reaction model Odor active compounds Odor active compounds Roasting Roasting Wuyi rock tea Wuyi rock tea

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GB/T 7714 Zhan, Sijia , Liu, Zhibin , Su, Weiying et al. Role of roasting in the formation of characteristic aroma of wuyi rock tea [J]. | FOOD CONTROL , 2023 , 147 .
MLA Zhan, Sijia et al. "Role of roasting in the formation of characteristic aroma of wuyi rock tea" . | FOOD CONTROL 147 (2023) .
APA Zhan, Sijia , Liu, Zhibin , Su, Weiying , Lin, Chih-Cheng , Ni, Li . Role of roasting in the formation of characteristic aroma of wuyi rock tea . | FOOD CONTROL , 2023 , 147 .
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