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学者姓名：施元基
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Abstract ：
The discovery of comammox bacteria has revolutionized our understanding of nitrification, challenging the conventional paradigm that this process is mediated by two distinct microbial groups. Although comammox bacteria, particularly Candidatus Nitrospira nitrosa, are prevalent with significant activities in wastewater treatment systems, their physiological and biochemical properties, particularly growth substrates-based kinetics, are yet to be fully disclosed. To this end, we first attempted to enrich Ca. N. nitrosa in a laboratory-scale continuous stirred tank reactor fed with mainstream-level ammonium. Following the 40 d operation, a suite of microbial analyses jointly confirmed the unprecedentedly rapid specific enrichment and absolute dominance of Ca. N. nitrosa with the entire gene repertoire needed for completing the full nitrification process in the sludge. Dedicated batch tests then revealed and validated the affinity constants of Ca. N. nitrosa for total ammonium, nitrite and oxygen (0.393 +/- 0.058 mg-N/L, 0.365 +/- 0.001 mg-N/L and 0.006 +/- 0.001 mg-O2/L, respectively), demonstrating high affinities for substrates that could underpin its widely reported competitive presence and functionality in wastewater treatment systems. This work provides the first kinetic characterization of Ca. N. nitrosa, complementing the currently limited understanding of the comammox process while enabling a better evaluation of its important role in the nitrogen cycle across diverse environments.
Keyword ：
Candidatus Nitrospira nitrosa Candidatus Nitrospira nitrosa completeammonium oxidation (comammox) completeammonium oxidation (comammox) continuous stirred tank reactor(CSTR) continuous stirred tank reactor(CSTR) kinetics kinetics wastewater treatment wastewater treatment
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| GB/T 7714 | Hou, Jiaying , Zhu, Ying , Shi, Yuanji et al. Enrichment and Kinetic Profiling of Candidatus Nitrospira nitrosa Culture Reveal Mechanisms Underlying Its Prevalence in Wastewater Treatment Systems [J]. | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (29) : 15272-15281 . |
| MLA | Hou, Jiaying et al. "Enrichment and Kinetic Profiling of Candidatus Nitrospira nitrosa Culture Reveal Mechanisms Underlying Its Prevalence in Wastewater Treatment Systems" . | ENVIRONMENTAL SCIENCE & TECHNOLOGY 59 . 29 (2025) : 15272-15281 . |
| APA | Hou, Jiaying , Zhu, Ying , Shi, Yuanji , Lin, Limin , Meng, Fangang , Xu, Meiying et al. Enrichment and Kinetic Profiling of Candidatus Nitrospira nitrosa Culture Reveal Mechanisms Underlying Its Prevalence in Wastewater Treatment Systems . | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2025 , 59 (29) , 15272-15281 . |
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Abstract ：
Despite the well-reported ability of ammonium-oxidizing bacteria (AOB) to cometabolize non-growth substrates, most relevant studies were substantiated with the ammonium oxidation activity as the driving force and didn't further explore/verify/distinguish the contribution of the hydroxylamine oxidation process of AOB. Therefore, using highly enriched AOB-dominated sludge, a series of dedicated batch tests were conducted in this work to investigate the hydroxylamine-based cometabolic degradation of chloroquine (CLQ), a widely applied drug with a significant environmental concern, and its associated impacts on the nitrogen conversions of AOB. The results indicated that the hydroxylamine oxidation process of AOB could trigger the degradation of CLQ which achieved 0.4-1.2 mg/L CLQ removal under the studied conditions, despite the potentially preferred utilization of CLQ degradation products towards mineralization by AOB over CLQ itself. CLQ and its degradation products wouldn't affect the hydroxylamine oxidation process or the associated N2O production through the hydroxylamine oxidation pathway of AOB. We hope this work will not only provide new perspectives for prospective research on the cometabolic capacity and features of AOB but also inspire the revisit of the literature-reported research on such an important topic.
Keyword ：
Ammonium-oxidizing bacteria (AOB) Ammonium-oxidizing bacteria (AOB) Chloroquine (CLQ) Chloroquine (CLQ) Cometabolism Cometabolism Hydroxylamine (NH2OH) Hydroxylamine (NH2OH) Nitrous oxide (N2O) Nitrous oxide (N2O)
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| GB/T 7714 | Chen, Bokai , Wang, Haojie , Shi, Yuanji et al. Hydroxylamine-based cometabolism of ammonium-oxidizing bacteria: chloroquine biodegradation and associated nitrogen conversions [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 385 . |
| MLA | Chen, Bokai et al. "Hydroxylamine-based cometabolism of ammonium-oxidizing bacteria: chloroquine biodegradation and associated nitrogen conversions" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 385 (2025) . |
| APA | Chen, Bokai , Wang, Haojie , Shi, Yuanji , Yang, Linyan , Ni, Bing-Jie , Chen, Xueming . Hydroxylamine-based cometabolism of ammonium-oxidizing bacteria: chloroquine biodegradation and associated nitrogen conversions . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 385 . |
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This work investigated the impact of hydrazine (N2H4), a key intermediate product of the anaerobic ammonium oxidation (anammox) process and a proposed additive to enhance the metabolism of anammox bacteria, on the hydroxylamine (NH2OH) oxidation process mediated by hydroxylamine oxidoreductase (HAO) in ammoniumoxidizing bacteria (AOB). Through batch tests using highly enriched AOB-dominated sludge, we demonstrated that N2H4 competitively inhibited NH2OH oxidation while significantly stimulating nitrous oxide (N2O) production. Molecular docking confirmed the competitive inhibition relationship between NH2OH and N2H4 for the same active sites of HAO, verifying the higher binding affinity of NH2OH (binding energy: -2.3 kcal/mol) compared with N2H4 (binding energy: -1.7 kcal/mol). The interference of N2H4 was further substantiated with dedicated batch tests initiated with NH4+. The competitive inhibition of <= 10 mg-N/L N2H4 was found to induce significant NH2OH accumulation, elevate N2O production and impair partial nitrification efficiency. This work raised not only awareness about the indirect negative impact of the anammox process on the activity and functionality of AOB but also concerns regarding the application of N2H4 to enhance the activity of anammox bacteria, particularly in partial nitrification/anammox systems.
Keyword ：
Ammonium-oxidizing bacteria (AOB) Ammonium-oxidizing bacteria (AOB) Hydrazine (N 2 H 4 ) Hydrazine (N 2 H 4 ) Hydroxylamine (NH 2 OH) Hydroxylamine (NH 2 OH) Hydroxylamine oxidoreductase (HAO) Hydroxylamine oxidoreductase (HAO) Nitrous oxide (N 2 O) Nitrous oxide (N 2 O)
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| GB/T 7714 | Wang, Haojie , Chen, Bokai , Shi, Yuanji et al. Competitive inhibition of hydrazine on hydroxylamine oxidation by ammonium-oxidizing bacteria and its stimulative impact on associated N2O production [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 516 . |
| MLA | Wang, Haojie et al. "Competitive inhibition of hydrazine on hydroxylamine oxidation by ammonium-oxidizing bacteria and its stimulative impact on associated N2O production" . | CHEMICAL ENGINEERING JOURNAL 516 (2025) . |
| APA | Wang, Haojie , Chen, Bokai , Shi, Yuanji , Yang, Linyan , Chen, Xueming . Competitive inhibition of hydrazine on hydroxylamine oxidation by ammonium-oxidizing bacteria and its stimulative impact on associated N2O production . | CHEMICAL ENGINEERING JOURNAL , 2025 , 516 . |
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In the face of the mass production and consumption of chloroquine (CLQ), the presence of CLQ has posed a significant threat to the water environment and living organisms due to its bio-accumulative and persistent nature. In this work, a heterogeneous electro-Fenton system using a pyrite-modified carbon felt as the cathode (EF-FeS2-CF) was constructed to remove CLQ. EF-FeS2-CF was found to achieve 92.4 +/- 0.1% removal of 5 mg/L CLQ after 30 min at 20 mA and exhibited an outstanding catalytic performance for CLQ removal in wide ranges of operating conditions, excellent recyclability with low iron leaching (>90% 30-min CLQ removal efficiency for all five cyclic experiments which led to only an accumulative Fe loss of <0.3%) and remarkable application potential in different aqueous environments without any pH adjustment. Both O-2(center dot-) and (OH)-O-center dot were found to greatly contribute to CLQ degradation while the latter played a major role (>50% CLQ removal). To describe CLQ degradation towards complete mineralization in EF-FeS2-CF, three CLQ degradation paths were proposed based on the main degradation intermediates identified with generally lower ecotoxicities. Such an EF-FeS2-CF system therefore presents promising application potential to treat CLQ-laden waters.
Keyword ：
Chloroquine Chloroquine Heterogeneous electro-Fenton Heterogeneous electro-Fenton Hydroxyl radical ( Hydroxyl radical ( OH) OH) Pyrite (FeS 2 ) Pyrite (FeS 2 )
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| GB/T 7714 | Zhang, Yuehua , Lin, Yinghui , Shi, Yuanji et al. Efficient degradation of chloroquine via pyrite-modified electro-Fenton yielding •OH and O2 •- [J]. | JOURNAL OF CLEANER PRODUCTION , 2024 , 483 . |
| MLA | Zhang, Yuehua et al. "Efficient degradation of chloroquine via pyrite-modified electro-Fenton yielding •OH and O2 •-" . | JOURNAL OF CLEANER PRODUCTION 483 (2024) . |
| APA | Zhang, Yuehua , Lin, Yinghui , Shi, Yuanji , Yang, Linyan , Ni, Bing-Jie , Chen, Xueming . Efficient degradation of chloroquine via pyrite-modified electro-Fenton yielding •OH and O2 •- . | JOURNAL OF CLEANER PRODUCTION , 2024 , 483 . |
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