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学者姓名：李梅金
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Abstract ：
The development of affordable and user-friendly diagnostic tools for early warning and monitoring progression of chronic kidney disease (CKD) is crucial to reducing CKD-related morbidity and mortality. This study reports on (1) a protein-templated AIEgen, Ir@BSA, which emits intense green phosphorescence with a quantum yield up to 69.40% and a lifetime up to 1839.40 ns in aqueous solution; (2) a straightforward protocol for Cys C quantitation, which employs Ir@BSA as the phosphorescent signal indicator and papain as the biomolecular recognition element, respectively; and (3) a smartphone-based portable phosphorescence reader (termed as SAPD), which can stably excite and accurately collect phosphorescence signals from the paper-based arrays. Quantitation of Cys C in clinical serum samples using SAPD integrated with the paper-based arrays highlights its remarkable advantages including high sensitivity (0.36 µg mL−1) and specificity, cost-effectiveness (∼$67.5 per set), portability (∼450 g), good precision (RSD ≤ 8.25 %), good accuracy (comparable to clinical standard latex immune-turbidimetric method), and high throughput (16 samples per experiment). More importantly, this study reveals the significant potential of Cys C as an early warning marker of CKD progression. The reported method enables Cys C quantitation anywhere, anytime, by anyone, and is ideally suited for mass screening for CKD and home monitoring of CKD progression, facilitating early diagnosis and proactive management of CKD. © 2025 The Author(s). Aggregate published by SCUT, AIEI and John Wiley & Sons Australia, Ltd.
Keyword ：
bovine serum albumin bovine serum albumin Ir complex Ir complex papain papain paper-based arrays paper-based arrays phosphorescence sensors phosphorescence sensors portable device portable device
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| GB/T 7714 | Xia, S. , Zhu, X. , Niu, S. et al. AIEgen-Based and Smartphone-Assisted On-Site Quantitation of Cystatin C for Monitoring of Chronic Kidney Disease [J]. | Aggregate , 2025 . |
| MLA | Xia, S. et al. "AIEgen-Based and Smartphone-Assisted On-Site Quantitation of Cystatin C for Monitoring of Chronic Kidney Disease" . | Aggregate (2025) . |
| APA | Xia, S. , Zhu, X. , Niu, S. , Zhang, W. , Gong, J. , Luo, Z. et al. AIEgen-Based and Smartphone-Assisted On-Site Quantitation of Cystatin C for Monitoring of Chronic Kidney Disease . | Aggregate , 2025 . |
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Alkaline phosphatase (ALP) is a vital protein catalyst that holds a central role in modulating protein metabolism and function throughout organisms, and serves as a reliable foundation for the treatment of human diseases. Thus, the development of a sensitive and selective detection strategy for ALP is considerable scientific and practical significance. In this study, SiO2 was chosen as the carrier for encapsulating Ru-1 luminophores to effectively prevent the leakage of Ru-1 molecules. Meanwhile, during the preparation process, the introduction of a poor solvent induces aggregation of Ru-1 molecules into nanoparticles, thereby enhancing emission through the aggregation-induced emission effect. Furthermore, using an in-situ growth method,we developed a phosphorescence-based detection strategy, which builds on Ru-SiO2-MnO2 nanocomposites. L-ascorbic acid (AA) can be generated by ALP, which catalyzes the hydrolysis of sodium L-ascorbyl-2-phosphate (AAP). This process further breaks down MnO2 and triggers the phosphorescence sensor through a "turn-on" mechanism. Moreover, by exploiting the capacity of Na3VO4 to suppress ALP activity, this phosphorescent method was further employed for the identification of ALP inhibitors. Under the optimized experimental conditions, the phosphorescent intensity displayed a linear association with ALP concentrations ranging from 0.05 to 17 U/L, while the limit of detection was as low as 0.031 U/L. The proposed strategy eliminates the need for complex washing or modification procedures and avoids the aggregation-caused quenching phenomenon of traditional organic dyes, thus standing out as an uncomplicated, economical "turn-on" phosphorescent detection method for ALP. This approach also demonstrated excellent applicability in the analysis of serum samples.
Keyword ：
Aggregation-induced emission Aggregation-induced emission Alkaline phosphatase Alkaline phosphatase Nanocomposites Nanocomposites Phosphorescence Phosphorescence Ru(II) complex Ru(II) complex
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| GB/T 7714 | Zheng, Qianghui , Zhang, Jinyuan , Li, Mei-Jin et al. Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors [J]. | MICROCHEMICAL JOURNAL , 2025 , 218 . |
| MLA | Zheng, Qianghui et al. "Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors" . | MICROCHEMICAL JOURNAL 218 (2025) . |
| APA | Zheng, Qianghui , Zhang, Jinyuan , Li, Mei-Jin , Fu, Fengfu . Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors . | MICROCHEMICAL JOURNAL , 2025 , 218 . |
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The judicious utilization of antibiotics has established a robust bulwark for human health. However, their improper usage has engendered deleterious ramifications on the environment, underscoring the imperative for developing efficacious and cost-effective detection and degradation platforms. This study presents a sulfur-modified iron-cobalt bimetallic single-atom nitrogen-doped carbon catalyst (S-FeCo-NC) with a noncopper active center. In contrast to conventional laccase, which utilizes copper as its active center, the S-FeCo-NC catalyst exhibits multiple enzyme activities, including laccase-like, peroxidase-like, and catalase-like functions, with iron and cobalt serving as the active centers. As a proof of concept, the combined laccase-like and catalase-like functions of S-FeCo-NC were used as independent signal outputs, while a multienzyme cascade dual-mode assay system was designed for the rapid detection of tetracycline (TC) in combination with peroxidase-like enzymes. In this system, oxygen directly participated in the catalytic process of laccase-like as an electron acceptor, while catalase-like peroxidase efficiently catalyzed the production of O2 from H2O2. The elevated concentration of O2 offered a unique advantage for the increased catalytic activity of the laccase-like enzyme, which outputs visually resolved colorimetric signals using stable 4-aminopyridine with oxidized TC. Furthermore, the peroxidase-like activity of S-FeCo-NC catalyzed the generation of OH radicals with strong oxidative properties, and these radicals carried out effective oxidative decomposition of TC. The signal output of the response of the catalytic process was performed using differential pulse cyclic voltammetry, which further improved the sensitivity and accuracy of the detection. The experimental findings demonstrate that the detection system exhibits a favorable response signal to TC within the range of 0.005-500 mu M, with its detection range reaching 0.5-500 and 0.005-1.00 mu M, respectively, and the detection limit is as low as 0.22 mu M and 1.68 nM, respectively. This cascade dual-mode detection system, based on multienzyme activity, has been shown to significantly enhance the catalytic activity of laccase, while also demonstrating stability in a lower detection range. This suggests that it may offer a novel approach for the sensitive detection and degradation of environmental pollutants.
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| GB/T 7714 | Wang, Yunsen , Tian, Shuo , Chen, Shuyun et al. S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7526-7535 . |
| MLA | Wang, Yunsen et al. "S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7526-7535 . |
| APA | Wang, Yunsen , Tian, Shuo , Chen, Shuyun , Li, Meijin , Tang, Dianping . S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7526-7535 . |
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Crystal facet engineering is a pivotal strategy to design high-performance photoelectrodes and suppress electron and hole complexation, thus enhancing photoelectrochemical (PEC) activity through carrier enrichment at specific crystal facets. However, there is still a lack of systematic resolution on the intrinsic principles of crystal facet tuning energy band structure and the specific adsorption of signaling molecules. In this work, a multidimensional synergistic optimization strategy was proposed to achieve precise prediction and targeted crystal facet design of photoelectrodes by establishing a quantitative structure-activity relationship (QSAR) model of "crystal configuration-molecular recognition-carrier transport". A three-dimensional hierarchical TiO2 nanoflower (3D HTNF) photoelectrode dominated by the {110} facet exhibited a significant positive photocurrent toward uric acid (UA). Integrated with a microelectromechanical system (MEMS), a miniaturized self-powered PEC biosensor provided an innovative solution for high-throughput, noninvasive UA monitoring in saliva and displayed a linear range of 0.01-50 mu M with a detection limit of 8.76 nM. In addition, the advantages of photoelectrodes in light harvesting, charge separation and migration, molecular adsorption, and surface reactions were verified by density functional theory (DFT) calculations to reveal the path selectivity and carrier transport mechanisms of the photo-oxidation reactions on specific crystal surfaces. This study elucidates the interplay mechanism of the crystal surface tuning energy band structure and the interfacial kinetics of response. The program can be extended to precisely detect biomarkers in complex biological matrices, promoting the leapfrog development of noninvasive health monitoring technology.
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| GB/T 7714 | Tian, Shuo , Yu, Zhichao , Wang, Yunsen et al. Crystal Facet Engineering Modulated Electron Transfer Mechanisms: A Self-Powered Photoelectrochemical Sensing Platform for Noninvasive Detection of Uric Acid [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (17) : 9518-9526 . |
| MLA | Tian, Shuo et al. "Crystal Facet Engineering Modulated Electron Transfer Mechanisms: A Self-Powered Photoelectrochemical Sensing Platform for Noninvasive Detection of Uric Acid" . | ANALYTICAL CHEMISTRY 97 . 17 (2025) : 9518-9526 . |
| APA | Tian, Shuo , Yu, Zhichao , Wang, Yunsen , Chen, Shuyun , Li, Meijin , Tang, Dianping . Crystal Facet Engineering Modulated Electron Transfer Mechanisms: A Self-Powered Photoelectrochemical Sensing Platform for Noninvasive Detection of Uric Acid . | ANALYTICAL CHEMISTRY , 2025 , 97 (17) , 9518-9526 . |
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This study presents a colorimetric sensor for cancer screening utilizing the bifunctional enzyme activity of NiCo Prussian blue analogue (PBA), a PBA material. By introducing oxygen vacancies and employing a dual-metal doping strategy, NiCo PBA overcomes the limitations in catalytic activity observed in single-metal-doped materials (such as Ni PBA and Co PBA), significantly enhancing both peroxidase-like (POD) and catalase-like (CAT) activities. Compared to single-metal-doped Ni PBA and Co PBA, NiCo PBA exhibited a 30.08-fold increase in POD activity and a 4.83-fold increase in CAT activity, demonstrating higher sensitivity in carcinoembryonic antigen (CEA) detection. By integrating NiCo PBA with a cascade catalytic reaction principle, we developed a highly efficient and sensitive CEA detection method. NiCo PBA was utilized as a catalytic material in this method. Under the action of glucose oxidase, the decomposition of hydrogen peroxide was catalyzed by NiCo PBA, and oxygen was generated. Furthermore, a blue flocculent substance was produced when NiCo PBA was reacted with a chromogenic substrate. Through mutual verification by these two methods, the quantitative determination of CEA in serum samples was achieved. The experimental results demonstrated that the POD-like activity detection range was 0.2-50 ng mL-1, with a limit of detection (LOD) of 0.061 ng mL-1, while the CAT-like activity detection range was 0.1-20 ng mL-1, with an LOD of 0.028 ng mL-1. The sensitivity of this method was substantially increased compared to monometallic materials. Furthermore, this strategy possesses good scalability and can be adapted for the detection of various analytes by replacing different recognition units, providing an efficient detection platform for early cancer screening.
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| GB/T 7714 | Wu, Di , Yu, Zhichao , Qin, Jiao et al. A Bimetallic Nanozyme Synergistic Effect-Driven Enzyme Cascade Nanoreactor for Instant Immunoassay [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (20) : 10947-10954 . |
| MLA | Wu, Di et al. "A Bimetallic Nanozyme Synergistic Effect-Driven Enzyme Cascade Nanoreactor for Instant Immunoassay" . | ANALYTICAL CHEMISTRY 97 . 20 (2025) : 10947-10954 . |
| APA | Wu, Di , Yu, Zhichao , Qin, Jiao , Li, Meijin , Tang, Dianping . A Bimetallic Nanozyme Synergistic Effect-Driven Enzyme Cascade Nanoreactor for Instant Immunoassay . | ANALYTICAL CHEMISTRY , 2025 , 97 (20) , 10947-10954 . |
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Nanozyme-based electrochemical biosensors have emerged as an alternative to enzyme-based biosensors for next-generation bioanalysis. However, potential antibody modifications limit the catalytic sites of the nanozyme, thereby reducing sensor sensitivity. Here, a sensitive method for determining carcinoembryonic antigen (CEA) was developed. It involved coupling a cascade enzyme-enzyme-like catalytic reaction using Fe-Co Prussian blue analog nanozymes with high peroxidase-like activity (79.42 U mg-1). Briefly, the transduction of biological signals to chemical signals was achieved through the strategy centered on catalytic electroactive probes. Thereafter, with the assistance of the microelectrochemical workstation, the output of signals was realized. The platform exhibited an ultra-wide range of 0.020-100 ng mL-1 and a detection limit of 0.013 ng mL-1 CEA, which was mainly attributed to the excellent peroxidase activity, good conductivity, and synergistic amplification of current signals of synthesized nanozymes. In addition, the modification-free features greatly reduced the complexity of the bioassay and significantly improves its portability and cost-effectiveness. Overall, this study advances the development of nanozymes and their electrochemical biosensing applications and is expected to extend to the development of miniaturized devices in direct detection environments.
Keyword ：
Bifunctional nanozyme Bifunctional nanozyme Carcinoembryonic antigen Carcinoembryonic antigen Catalytic amplification Catalytic amplification Electrochemical immunoassay Electrochemical immunoassay
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| GB/T 7714 | Chen, Shuyun , Tian, Shuo , Wang, Yunsen et al. Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
| MLA | Chen, Shuyun et al. "Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 278 (2025) . |
| APA | Chen, Shuyun , Tian, Shuo , Wang, Yunsen , Li, Meijin , Tang, Dianping . Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
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Sluggish charge transfer and rapid electron-hole recombination severely limit the analytical performance of photoelectrochemical (PEC) immunoassays. This work presented a PEC immunosensing strategy that employed a target-induced enzyme-catalyzed reaction to in-situ generate oxygen vacancy (Ov) for amplifying the photocurrent detection of carcinoembryonic antigen (CEA). Concretely, ascorbic acid-2-phosphate (AAP) was catalyzed to produce ascorbic acid (AA) by alkaline phosphatase (ALP) in the presence of CEA. The generated AA could serve as a reducing agent to introduce oxygen vacancy (Ov) into the etching tungsten trioxide (E-WO3) photoanode, resulting in an Ov-enriched E-WO3 (E-WO3-Ov) photoanode. The formation of Ov allowed efficient introduction of defect levels into the energy band structure of E-WO3-Ov photoanode, resulting in high charge transfer and electron-hole separation efficiency for photocurrent amplification. Later, it was applied to fabricate a PEC immunosensor, thus enabling a wide linear range from 0.02 to 80 ng/mL and a low detection limit of 12.9 pg/mL. Overall, this work presented a promising sensing strategy for PEC immunosensors, expanding the scope of potential applications in bioassays and clinical diagnostics.
Keyword ：
Charge transfer Charge transfer Defect level Defect level Electron-hole separation Electron-hole separation Oxygen vacancy Oxygen vacancy Photoelectrochemical immunoassay Photoelectrochemical immunoassay
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| GB/T 7714 | Qin, Jiao , Yu, Zhichao , Wu, Di et al. Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
| MLA | Qin, Jiao et al. "Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 279 (2025) . |
| APA | Qin, Jiao , Yu, Zhichao , Wu, Di , Li, Meijin , Tang, Dianping . Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
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A highly charged water-soluble iridium(III) (Ir(III)) complex [(ppy)2Ir(bpy-N)]3+ was designed, synthesized and characterized, and the crystal structure of it was determined by X-ray crystallography. The photophysical properties of complex in absence or presence of sodium heparin were studied by UV-vis absorption and photoluminescent spectroscopies. After addition of sodium heparin, the luminescent intensity of complex at ca. 656 nm was increased about 4.3-fold, without any observable color change in the solution. The new Ir(III) complex was also assembled with gold nanoparticles (AuNPs) for the dual-mode detection of sodium heparin, combined with colorimetric and luminescent methods. In this system, the luminescence of complex was quenched by AuNPs firstly with the red color of gold nano colloids transition into blue due to the aggregation. The color of the assembled system changed from blue to purple to red accompanying with the luminescent intensity of system restoring after increasing the concentrations of added sodium heparin. The sodium heparin was sensitively detected with LOD of 0.09 mu g/mL. This work demonstrates a novel bifunctional sensing platform for sodium heparin, leveraging the unique photophysical properties of the Ir(III) complex and the plasmonic behavior of AuNPs, offering significant potential for sensitive and visual detection applications.
Keyword ：
Colorimetric sensing Colorimetric sensing Gold nanoparticles Gold nanoparticles Iridium(III) complex Iridium(III) complex photoluminescence photoluminescence Sodium heparin Sodium heparin
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| GB/T 7714 | Lin, Jinshan , Wang, Shengjie , Jiao, Pengchong et al. Colorimetric and luminescent determination of heparin based on assembly between a highly charged iridium(III) complex and gold nanoparticles [J]. | JOURNAL OF MOLECULAR STRUCTURE , 2025 , 1337 . |
| MLA | Lin, Jinshan et al. "Colorimetric and luminescent determination of heparin based on assembly between a highly charged iridium(III) complex and gold nanoparticles" . | JOURNAL OF MOLECULAR STRUCTURE 1337 (2025) . |
| APA | Lin, Jinshan , Wang, Shengjie , Jiao, Pengchong , Li, Mei-Jin . Colorimetric and luminescent determination of heparin based on assembly between a highly charged iridium(III) complex and gold nanoparticles . | JOURNAL OF MOLECULAR STRUCTURE , 2025 , 1337 . |
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S: Mercury ions (Hg2+) are highly toxic and prone to bioaccumulation, showing a strong attraction to proteins and enzymes that contain sulfur. Even minute quantities of Hg2+ can lead to severe health issues. Given that mitochondria are a primary target organelle of Hg2+, it is essential to create a probe that can accurately detect Hg2+ within intracellular mitochondria. In this study, we developed two innovative Ir(III) complex probes that emit near-infrared light. The crystal structure of Ir2 was determined using X-ray techniques, which reveals that Ir2 contains a pyridine group capable of recognizing Hg2+ and targeting mitochondria, allowing for the precise identification of Hg2+ both in vitro and within the mitochondria of living cells. Additionally, these two novel near-infrared phosphorescent Ir(III) complexes demonstrate significant capabilities in producing ROS including singlet oxygen, center dot O-2(-) and center dot OH, which renders them effective photosensitizers under visible light exposure for photodynamic therapy (PDT). This research offers a promising approach for detecting Hg2+ in vitro and in the mitochondrial microenvironment of living cells, which have some implications for the future development of pertinent transition metal complexes for mitochondria-targeted photodynamic therapy in cancer cells.
Keyword ：
Hg2+ sensing Hg2+ sensing Ir(III) complexes Ir(III) complexes Luminescence imaging Luminescence imaging Near-infrared phosphorescence Near-infrared phosphorescence Photodynamic therapy Photodynamic therapy
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| GB/T 7714 | Liu, Jie , Chen, Meihua , Li, Mei-Jin . Mitochondria-targeted and near-infrared phosphorescent Ir(III) complexes for specific detection of Hg2+ and photodynamic therapy [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 337 . |
| MLA | Liu, Jie et al. "Mitochondria-targeted and near-infrared phosphorescent Ir(III) complexes for specific detection of Hg2+ and photodynamic therapy" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 337 (2025) . |
| APA | Liu, Jie , Chen, Meihua , Li, Mei-Jin . Mitochondria-targeted and near-infrared phosphorescent Ir(III) complexes for specific detection of Hg2+ and photodynamic therapy . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 337 . |
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In recent years, the optical functional materials of iridium(III) complexes have attracted much attention due to their excellent photophysical properties and potential biomedical applications. Among them, aggregation-induced emission (AIE) properties have shown significant advantages in photodynamic therapy (PDT). In this work, three new iridium(III) complexes with near-infrared emission were designed and synthesized, and their crystal structures were determined. All complexes exhibited significant AIE phenomena. Especially, Ir-1 containing an -NH2 functional group exhibited a unique hollow nanoparticle morphology, excellent mitochondrial targeting ability, and high reactive oxygen species (ROS) efficiency, which is expected to play an important role as a new type of nanoprobe in the field of PDT. The complex has the ability to generate both type I and type II ROS, which makes it a potent photosensitizer for the efficient phototherapy of hypoxic tumors. What is more, the complex has dual functions of PDT and chemotherapy, and their synergistic effect greatly enhances the antineoplastic effects of the reagent.
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| GB/T 7714 | Chen, Weibin , Zheng, Qianghui , Zhang, Wanqing et al. Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy [J]. | INORGANIC CHEMISTRY , 2025 , 64 (28) : 14487-14497 . |
| MLA | Chen, Weibin et al. "Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy" . | INORGANIC CHEMISTRY 64 . 28 (2025) : 14487-14497 . |
| APA | Chen, Weibin , Zheng, Qianghui , Zhang, Wanqing , Fu, Fengfu , Li, Mei-Jin . Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy . | INORGANIC CHEMISTRY , 2025 , 64 (28) , 14487-14497 . |
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