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学者姓名：魏峥
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Abstract ：
Dried leech (Whitmania pigra whitman) has been widely used as a traditional animal-based Chinese medicine. Dried leech extracts have been reported to have various biological activities that are often associated with mammalian glycosaminoglycans. However, their presence and possible structural characteristics within dried leech were previously unknown. In this study, glycosaminoglycans were isolated from dried leech for the first time and their structures were analyzed by the combination of Fourier-transform infrared spectroscopy, liquid chromatography-ion trap/time-of-flight mass spectrometry and polyacrylamide gel electrophoresis. Heparan sulfate and chondroitin sulfate/dermatan sulfate were detected in dried leech with varied disaccharide compositions and possess a heterogeneous structure. Heparan sulfate species possess an equal amount of total 2-O-sulfated, N-sulfated and acetylated disaccharides, while chondroitin sulfate /dermatan sulfate contain high content of 4-O-sulfated disaccharides. Also, the quantitative analysis revealed that the contents of heparan sulfate and chondroitin/dermatan sulfate in dried leech varied significantly, with chondroitin/dermatan sulfate being by far the most abundant. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of the dried leech. Furthermore, leech glycosaminoglycans showed a strong ABTS radical scavenging ability, which suggests the potential of leech polysaccharides for exploitation in the nutraceutical and pharmaceutical industries.
Keyword ：
Antioxidant activities Antioxidant activities Dried leech Dried leech Glycosaminoglycans Glycosaminoglycans Isolation Isolation Structural characterization Structural characterization
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| GB/T 7714 | Shen, Tao , Wang, Shangteng , Liang, Quntao et al. Characterization and antioxidant activities of glycosaminoglycans from dried leech [J]. | GLYCOCONJUGATE JOURNAL , 2023 , 40 (2) : 169-178 . |
| MLA | Shen, Tao et al. "Characterization and antioxidant activities of glycosaminoglycans from dried leech" . | GLYCOCONJUGATE JOURNAL 40 . 2 (2023) : 169-178 . |
| APA | Shen, Tao , Wang, Shangteng , Liang, Quntao , Sharp, Joshua S. , Wei, Zheng . Characterization and antioxidant activities of glycosaminoglycans from dried leech . | GLYCOCONJUGATE JOURNAL , 2023 , 40 (2) , 169-178 . |
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为了进一步探讨非还原端饱和结构的肝素寡糖在UV 232 nm的吸收情况,制备了4种饱和结构的肝素二糖,并用离子对反相液相色谱/离子阱飞行时间质谱(RPIP-LC/MS-IT-TOF)光电二极管阵列检测器分析了它们在UV 232 nm的吸收情况.分析结果表明,饱和结构的肝素二糖在UV 232 nm的检出限为9μg(S/N=10),UV232 nm/UV 206 nm约为不饱和结构肝素二糖UV 232 nm的7%～40%.结果还表明,肝素二糖UV 232 nm的吸收强度受亚硫酸基团(SO_3~(2-))影响较大.另外,通过比较不饱和结构的肝素/硫酸类肝素(Hep/HS)标样二糖发现,含N-未取代葡...
Keyword ：
测序 测序 紫外吸收 紫外吸收 肝素 肝素 质谱 质谱 饱和结构 饱和结构
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| GB/T 7714 | 梁群焘 , 邹强 , 林江慧 et al. 肝素非还原端饱和结构的紫外吸收研究及在肝素寡糖测序中的应用 [J]. | 高等学校化学学报 , 2021 , 42 (06) : 1776-1784 . |
| MLA | 梁群焘 et al. "肝素非还原端饱和结构的紫外吸收研究及在肝素寡糖测序中的应用" . | 高等学校化学学报 42 . 06 (2021) : 1776-1784 . |
| APA | 梁群焘 , 邹强 , 林江慧 , 刘树滔 , 魏峥 . 肝素非还原端饱和结构的紫外吸收研究及在肝素寡糖测序中的应用 . | 高等学校化学学报 , 2021 , 42 (06) , 1776-1784 . |
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Abstract ：
It is well known that the saturated structure in the non-reducing end (NRE) of heparin/heparan sulfate (HS) oligosaccharides is lacking ultraviolet (UV) 232 nm absorbance (normally used for specific detection of C4-C5 unsaturated oligosaccharides), which poses a significant challenge for structure analysis. However, we found that saturated heparin oligosaccharides can be detected at UV 232 nm with sufficient sensitivity in our previous research. In order to further study the UV 232 nm absorbance of the saturated structure, four saturated heparin disaccharides were prepared, and then their UV 232 nm absorptions were analyzed by ion-pair reversed-phase liquid chromatography ion-trap/time-of-flight mass spectrometry (RPIP-LC/MS-IT-TOF) with photodiode array detector. The results show that the saturated heparin disaccharides can be detected with sufficient sensitivity by absorbance at UV 232 nm, which still with 7%-40% of UV 232 nm intensity of heparin unsaturated disaccharides. It seems that the UV 232 nm intensity might be affected by the sulfate groups in the disaccharides. In addition, comparing to the common unsaturated heparin/HS disaccharides as references, the disaccharides with GlcNH(3)(+) residues also showed less sensitivity at UV 232 nm. Finally, we simplified the sequencing method of the N-unsubstituted dp6 oligosaccharides by a simple UV detection method, combined with pH 4.0 HNO2 scission and RPIP-LC/MS-IT-TOF analysis. This strategy offers possibilities for sequencing at N-sulfated residues using alternative pH 1.5 HNO2 scission.
Keyword ：
Heparin Heparin Mass spectrometry Mass spectrometry Saturated structure Saturated structure Sequencing Sequencing Ultraviolet absorption Ultraviolet absorption
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| GB/T 7714 | Liang Quntao , Zou Qiang , LiN Jianghui et al. Ultraviolet Absorbance of Saturated Structure in the Non-reducing End of Heparin/Heparan Sulfate Disaccharides and Its Application in Sequence of Heparin Oligosaccharides [J]. | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE , 2021 , 42 (6) : 1776-1784 . |
| MLA | Liang Quntao et al. "Ultraviolet Absorbance of Saturated Structure in the Non-reducing End of Heparin/Heparan Sulfate Disaccharides and Its Application in Sequence of Heparin Oligosaccharides" . | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 42 . 6 (2021) : 1776-1784 . |
| APA | Liang Quntao , Zou Qiang , LiN Jianghui , Liu Shutao , Wei Zheng . Ultraviolet Absorbance of Saturated Structure in the Non-reducing End of Heparin/Heparan Sulfate Disaccharides and Its Application in Sequence of Heparin Oligosaccharides . | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE , 2021 , 42 (6) , 1776-1784 . |
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Oviductus ranae (O.ran.) has been widely used as a tonic and a traditional animal-based Chinese medicine. O.ran. extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic. Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities. However, their presence and possible structural characteristics within O.ran. were previously unknown. In this study, glycosaminoglycans were isolated from O.ran. and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran. with varied disaccharide compositions. HS species contain highly acetylated disaccharides, and have various structures in their constituent chains. CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran..
Keyword ：
Chondroitin sulfate Chondroitin sulfate Dermatan sulfate Dermatan sulfate Hyaluronic acid Hyaluronic acid LC-MS-ITTOF LC-MS-ITTOF Oviductus ranae Oviductus ranae
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| GB/T 7714 | Huang, Haiyue , Mao, Jin , Liang, Quntao et al. Structural analysis of glycosaminoglycans from Oviductus ranae [J]. | GLYCOCONJUGATE JOURNAL , 2021 , 38 (1) : 25-33 . |
| MLA | Huang, Haiyue et al. "Structural analysis of glycosaminoglycans from Oviductus ranae" . | GLYCOCONJUGATE JOURNAL 38 . 1 (2021) : 25-33 . |
| APA | Huang, Haiyue , Mao, Jin , Liang, Quntao , Lin, Jianghui , Jiang, Lilong , Liu, Shutao et al. Structural analysis of glycosaminoglycans from Oviductus ranae . | GLYCOCONJUGATE JOURNAL , 2021 , 38 (1) , 25-33 . |
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Abstract ：
肝素/硫酸乙酰肝素(Hep/HS)寡糖结构多样,且与生物学、病理学功能息息相关,表征这些结构对解析它们的功能具有重要意义。本实验以市售猪肠肝素钠为原料,制备了9种肝素四糖,并用二糖组分分析方法结合反相离子对试剂色谱-串联离子阱飞行时间质谱(IPRP-LC/IT-TOF MS)进行结构解析。结果表明:9种肝素四糖中有两组同分异构体,且含己胺离子对试剂的RP-LC/MS能够成功分离肝素寡糖及其同分异构体;采用LC-MS/MS方法可以鉴定其中一组同分异构体的结构。该方法可为HS/Hep寡糖的研究提供标准品,并为解析HS/Hep同分异构体结构提供一种简单有效的方法,进而为更好地理解生物体内HS/Hep...
Keyword ：
同分异构体 同分异构体 四糖 四糖 液相色谱-串联质谱(LC-MS/MS) 液相色谱-串联质谱(LC-MS/MS) 肝素/硫酸类肝素 肝素/硫酸类肝素
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| GB/T 7714 | 杜佳燕 , 苏晓明 , 梁群焘 et al. 肝素/硫酸乙酰肝素四糖同分异构体的制备与表征 [J]. | 质谱学报 , 2020 , 41 (01) : 28-38 . |
| MLA | 杜佳燕 et al. "肝素/硫酸乙酰肝素四糖同分异构体的制备与表征" . | 质谱学报 41 . 01 (2020) : 28-38 . |
| APA | 杜佳燕 , 苏晓明 , 梁群焘 , 邹强 , 魏峥 . 肝素/硫酸乙酰肝素四糖同分异构体的制备与表征 . | 质谱学报 , 2020 , 41 (01) , 28-38 . |
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Heparin (Hep) and heparan sulfate (HS) are complex, sulfated GAGs consist of repeating disaccharide units each composed of a uronic acid, either glucuronate or iduronate [the latter sometimes sulfated at C-2, i.e. IdoA2S], and a derivative of glucosamine [either N-acetylglucosamine, N-sulfated glucosamine, or unsubstituted glucosamine] that can be variably O-sulfated. Hep/HS has important biological functions in developmental processes, angiogenesis, blood coagulation, cell adhesion, and tumour metastasis. These involve interactions with a wide variety of proteins, i.e. enzymes, cytokines, growth factors and extracellular matrix proteins through their specific sequences, patterns or densities of sulfation. Mass spectroscopy is now becoming a much more useful tool for the structural analysis of Hep/HS oligosaccharides because of its high detection sensitivity and molecular specificity. MS/MS of heparin/HS oligosaccharides can produce information-rich glycosidic and cross-ring product ions which can be used to determine the sites of acetylation or sulfation. In particular, LC-MS clearly has the potential now to sequence most oligosaccharides, especially with the recent development of a computational framework for dealing with the complex data generated. In this study, porcine intestines heparin was digested by heparinase I. The obtained crude tetrasaccharides (dp4s) were separated and further purified by strong anion-exchange HPLC and size-exclusion HPLC. The structures of dp4s were then investigated by the combination of disaccharide analysis, reversed-phase liquid chromatography/electrospray ionization ion trap/time-of-flight mass spectrometry (RP-LC/ESI-IT/TOF MS) and a computational simulation method. The results showed that nine tetrasaccharides (dp4s) were obtained, in which two series of dp4s isomer were existed. The dp4 isomers were successfully separated using IPRP-LC/MS system with hexylamine. One series of dp4s contains HexA(2S)-GlcNS and HexA(2S)-GlcNS(6S) disaccharides, and other ones possess HexA-GlcNS(6S) and HexA(2S)-GlcNS(6S). Because MSn analysis could produce abundant structurally useful fragments, the former dp4 isomers were selected to investigate fragmentation patterns for the further potential structure analysis by MS. MS2 analysis indicated that one dp4 was HexA(2S)-GlcNS- HexA(2S)-GlcNS(6S) which had one glycosidic cleavage Y1, two cross-ring cleavages 0,2A2 and 0,3A2 in the first glucosamine residues (from the non-reducing end) of oligosaccharide. In contrast, the other dp4 was HexA(2S)-GlcNS(6S)-HexA(2S)-GlcNS which contained two glycosidic cleavages B2 and Y1, two cross-ring cleavages 1,4A2 and 0,3A2 in the first glucosamine residues, and a cross-ring cleavages 1,5X4 in the first glucuronic acid residues of the oligosaccharide. This study provides a simple and efficient method for identifying the sequences of the Hep/HS oligosaccharide isomers, which might lead to a greater understanding of the biological roles of Hep/HS oligosaccharides in organisms. © 2020, Editorial Board of Journal of Chinese Mass Spectrometry Society. All right reserved.
Keyword ：
Acetylation Acetylation Cell adhesion Cell adhesion Drug products Drug products Glucosamine Glucosamine Glucose Glucose Hydraulic structures Hydraulic structures Ionization of liquids Ionization of liquids Ions Ions Isomers Isomers Liquid chromatography Liquid chromatography Mass spectrometry Mass spectrometry Oligosaccharides Oligosaccharides Plants (botany) Plants (botany) Polysaccharides Polysaccharides Proteins Proteins Sulfur compounds Sulfur compounds
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| GB/T 7714 | Du, Jia-Yan , Su, Xiao-Ming , Liang, Qun-Tao et al. Preparation and Characterization of Heparin/Heparan Sulfate Tetrasaccharide Isomers [J]. | Journal of Chinese Mass Spectrometry Society , 2020 , 41 (1) : 28-38 . |
| MLA | Du, Jia-Yan et al. "Preparation and Characterization of Heparin/Heparan Sulfate Tetrasaccharide Isomers" . | Journal of Chinese Mass Spectrometry Society 41 . 1 (2020) : 28-38 . |
| APA | Du, Jia-Yan , Su, Xiao-Ming , Liang, Qun-Tao , Zou, Qiang , Wei, Zheng . Preparation and Characterization of Heparin/Heparan Sulfate Tetrasaccharide Isomers . | Journal of Chinese Mass Spectrometry Society , 2020 , 41 (1) , 28-38 . |
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Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine. Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their possible structural characteristics in CCA are not clear. In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography. The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths. The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Keyword ：
Chondroitin sulfate; Colla corii asini; Dermatan sulfate; Hyaluronic acid; LC-MS-ITTOF Chondroitin sulfate; Colla corii asini; Dermatan sulfate; Hyaluronic acid; LC-MS-ITTOF
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| GB/T 7714 | Huang, H. , Liu, S. , Du, J. et al. Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry [J]. | Glycoconjugate Journal , 2020 , 37 (2) : 201-207 . |
| MLA | Huang, H. et al. "Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry" . | Glycoconjugate Journal 37 . 2 (2020) : 201-207 . |
| APA | Huang, H. , Liu, S. , Du, J. , Lin, J. , Liang, Q. , Wei, Z. . Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry . | Glycoconjugate Journal , 2020 , 37 (2) , 201-207 . |
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肝素和硫酸乙酰肝素由重复的二糖单元组成,其组分和结构与重要的生物病理、生理作用息息相关。这些二糖单元包括含量较高的N-硫酸化葡萄糖胺、N-乙酰化葡萄糖胺和含量较低的N-非取代葡萄糖胺残基。本研究采用2-氨基吖啶酮标记二糖,结合反相液相色谱-电喷雾-离子阱-飞行时间质谱法(RP-LC-ESI-IT-TOF MS)分析肝素/硫酸乙酰肝素中N-非取代二糖。通过优化检测条件,实现了12种肝素/硫酸乙酰肝素二糖的基线分离,并采用外标法相对定量分析各二糖组分。该方法适用于分析含有N-非取代二糖的肝素衍生物,可为进一步研究N-非取代葡萄糖胺的结构与功能提供检测方法,有助于更好地理解N-非取代葡萄糖胺残基在人...
Keyword ：
2-氨基吖啶酮 2-氨基吖啶酮 N-非取代二糖 N-非取代二糖 反相液相色谱-电喷雾-离子阱-飞行时间质谱(RP-LC-ESI-IT-TOF MS) 反相液相色谱-电喷雾-离子阱-飞行时间质谱(RP-LC-ESI-IT-TOF MS) 硫酸乙酰肝素 硫酸乙酰肝素
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| GB/T 7714 | 杜佳燕 , 黄海月 , 苏晓明 et al. 反相液相色谱-电喷雾-离子阱-飞行时间质谱法定量分析N-非取代肝素/硫酸乙酰肝素 [J]. | 质谱学报 , 2019 , 40 (03) : 222-232 . |
| MLA | 杜佳燕 et al. "反相液相色谱-电喷雾-离子阱-飞行时间质谱法定量分析N-非取代肝素/硫酸乙酰肝素" . | 质谱学报 40 . 03 (2019) : 222-232 . |
| APA | 杜佳燕 , 黄海月 , 苏晓明 , 魏峥 . 反相液相色谱-电喷雾-离子阱-飞行时间质谱法定量分析N-非取代肝素/硫酸乙酰肝素 . | 质谱学报 , 2019 , 40 (03) , 222-232 . |
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Alkaline-earth dihalide nanocrystals (NCs) such as SrFCl, owing to their high chemical stability and low phonon energy, are excellent host materials for lanthanide (Ln(3+)) doping to achieve desirable optical properties for various bioapplications. Herein, we report a novel strategy for the synthesis of sub-10 nm Ln(3+)-doped SrFCl NCs with efficient upconverting and downshifting luminescence through a facile one-step hot-injection method. Utilizing the temperature-dependent upconverting luminescence (UCL) from the thermally coupled H-2(11/2) and S-4(3/2) levels of Er3+, we showed the potential of SrFCl:Yb, Er NCs as sensitive UCL nanoprobes for non-contact thermal sensing with a maximum detection sensitivity of 0.0066 K-1, which is among the highest values for thermal sensing based on Er3+-activated UCL nanoprobes. Furthermore, by employing the intense downshifting luminescence from Tb3+ and Eu3+, we demonstrated the successful use of biotinylated SrFCl:Ce, Tb and SrFCl:Eu3+ nanoprobes for biotin receptor-targeted cancer cell imaging, thus revealing the great promise of SrFCl:Ln(3+) nanoprobes for versatile bioapplications. (C) 2019 Chinese Society of Rare Earths. Published by Elsevier B.V. All rights reserved.
Keyword ：
Bioimaging Bioimaging Lanthanide Lanthanide Rare earths Rare earths SrFCl nanocrystals SrFCl nanocrystals Thermal sensing Thermal sensing Upconverting luminescence Upconverting luminescence
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| GB/T 7714 | Wei, Jiaojiao , Lian, Wei , Zheng, Wei et al. Sub-10 nm lanthanide-doped SrFCl nanoprobes: Controlled synthesis, optical properties and bioimaging [J]. | JOURNAL OF RARE EARTHS , 2019 , 37 (7) : 691-698 . |
| MLA | Wei, Jiaojiao et al. "Sub-10 nm lanthanide-doped SrFCl nanoprobes: Controlled synthesis, optical properties and bioimaging" . | JOURNAL OF RARE EARTHS 37 . 7 (2019) : 691-698 . |
| APA | Wei, Jiaojiao , Lian, Wei , Zheng, Wei , Shang, Xiaoying , Zhang, Meiran , Dai, Tao et al. Sub-10 nm lanthanide-doped SrFCl nanoprobes: Controlled synthesis, optical properties and bioimaging . | JOURNAL OF RARE EARTHS , 2019 , 37 (7) , 691-698 . |
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Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presence within CCA, and their possible structural characteristics, were previously unknown. In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities. The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures. This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Keyword ：
Colla corii asini; Heparan sulfate; Heparin; LC-MS-ITTOF; N-unsubstituted disaccharide Colla corii asini; Heparan sulfate; Heparin; LC-MS-ITTOF; N-unsubstituted disaccharide
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| GB/T 7714 | Du, J. , Liu, S. , Liang, Q. et al. Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry [J]. | Glycoconjugate Journal , 2019 , 36 (3) : 211-218 . |
| MLA | Du, J. et al. "Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry" . | Glycoconjugate Journal 36 . 3 (2019) : 211-218 . |
| APA | Du, J. , Liu, S. , Liang, Q. , Lin, J. , Jiang, L. , Chen, F. et al. Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry . | Glycoconjugate Journal , 2019 , 36 (3) , 211-218 . |
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