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学者姓名：许炼
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Abstract ：
Hydroxytyrosol, a naturally occurring chemical with antioxidant and antiviral properties, is widely used in the nutrition, pharmaceutical, and cosmetic industries. In the present study, a modularized cascade composed of Modules 1 and 2 was designed and implemented to convert L-tyrosine to hydroxytyrosol. Module 1 was a fourenzymatic cascade for converting L-tyrosine to tyrosol. Engineering Module 1 by fine-tuning the expression of the desired enzymes resulted in a robust whole-cell catalyst, BL21 (M1-13), which converted L-tyrosine to tyrosol at high substrate loading. Module 2 involved a 4-hydroxyphenylacetate 3-monooxygenase (HpaBC)-catalyzed reaction to hydroxylate tyrosol to form hydroxytyrosol. The rational design of the HpaB subunit led to a positive variant, HpaB-Mu (T292S/R474A), which was subsequently applied to Module 2 for tyrosol hydroxylation, yielding a robust whole-cell catalyst, BL21 (M2-05). The two designed modules were merged for one-pot conversion of L-tyrosine to hydroxytyrosol by adjusting the ratio and total amount of whole-cell catalyst loading, capable of converting 40 mM of L-tyrosine to 35.8 mM of hydroxytyrosol with a high space-time yield (1.38 g/L/ h). The current study proved that engineering HpaB at the substrate tunnel was a feasible way to boost its activity and proposed an effective method for synthesizing hydroxytyrosol from low-cost substrates, which has great economic potential.
Keyword ：
Enzymatic cascade Enzymatic cascade HpaB HpaB Hydroxytyrosol Hydroxytyrosol Rational design Rational design
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| GB/T 7714 | Liu, Wen-Kai , Ren, Xiu-Xin , Xu, Lian et al. Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol [J]. | BIOORGANIC CHEMISTRY , 2025 , 155 . |
| MLA | Liu, Wen-Kai et al. "Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol" . | BIOORGANIC CHEMISTRY 155 (2025) . |
| APA | Liu, Wen-Kai , Ren, Xiu-Xin , Xu, Lian , Lin, Juan . Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol . | BIOORGANIC CHEMISTRY , 2025 , 155 . |
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Baeyer-Villiger monooxygenases (BVMOs) can catalyze the asymmetric sulfoxidation to form pharmaceutical prazoles in environmentally friendly approach. In this work, the thermostable BVMO named PockeMO had high sulfoxidation activity towards rabeprazole sulfide to form (R)-rabeprazole but demonstrated significant overoxidation activity to form undesired sulfone by-product. To address this issue, the enzyme was engineered based on the computer assisted comparison for the substrate binding conformations. A mutant S482Y/L532Q (MU2) was obtained with much alleviated overoxidation activity and enhanced sulfoxidation activity towards rabeprazole sulfide. The catalytic efficiency for (R)-rabeprazole oxidation decreased 35 folds and increased 40 folds for the sulfoxidation of lansoprazole sulfide. The structural mechanism for the selectivity improvement was illuminated to find a selectivity decision pocket that was conservatively present in BVMOs composed of 4 loops. In upscaled reaction system, the substrate loading for MU2 increased from 40 mM to 100 mM for the synthesis of enantiopure (R)-rabeprazole. The sulfone content decreased from 16.6 % to 1.2 % compared to PockeMO. Lansoprazole sulfide could also be fully converted into enantiopure (R)-lansoprazole at 50 mM in 4 h by MU2 while PockeMO almost did not have activity. This work indicated the synthetic applicability of MU2 for active pharmaceutical (R)-prazoles.
Keyword ：
Baeyer-Villiger monooxygenase Baeyer-Villiger monooxygenase ( R )-prazole ( R )-prazole Substrate selectivity Substrate selectivity
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| GB/T 7714 | Xu, Xinqi , Xu, Fahui , Chen, Jingjing et al. Engineering of a (R)-selective Baeyer-Villiger monooxygenase to minimize overoxidation activity for asymmetric synthesis of active pharmaceutical prazoles [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 296 . |
| MLA | Xu, Xinqi et al. "Engineering of a (R)-selective Baeyer-Villiger monooxygenase to minimize overoxidation activity for asymmetric synthesis of active pharmaceutical prazoles" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 296 (2025) . |
| APA | Xu, Xinqi , Xu, Fahui , Chen, Jingjing , Wang, Shumin , Wang, Xialian , Su, Bingmei et al. Engineering of a (R)-selective Baeyer-Villiger monooxygenase to minimize overoxidation activity for asymmetric synthesis of active pharmaceutical prazoles . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 296 . |
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The synthesis of steroids is challenging through multistep steroidal core modifications with high site-selectivity and productivity. In this work, a novel enzymatic cascade system was constructed for synthesis of testolactone by specific C17 lactonization/Δ1-dehydrogenation from inexpensive androstenedione using an engineered polycyclic ketone monooxygenase (PockeMO) and an appropriate 3-ketosteroid-Δ1-dehydrogenase (ReKstD). The focused saturation mutagenesis in the substrate binding pocket was implemented for evolution of PockeMO to eliminate the bottleneck effect. A best mutant MU3 (I225L/L226V/L532Y) was obtained with 20-fold higher specific activity compared to PockeMO. The catalytic efficiency (kcat/Km) of MU3 was 171-fold higher and the substrate scope shifted to polycyclic ketones. Molecular dynamic simulations suggested that the activity was improved by stabilization of the pre-lactonization state and generation of productive orientation of 4-AD mediated by distal L532Y mutation. Based on that, the three genes, MU3, ReKstD and a ketoreductase for NADPH regeneration, were rationally integrated in one cell via expression fine-tuning to form the efficient single cell catalyst E. coli S9. The single whole-cell biocatalytic process was scaled up and could generate 9.0 g/L testolactone with the high space time yield of 1 g/L/h without steroidal by-product, indicating the potential for site-specific and one-pot synthesis of steroid. © 2024
Keyword ：
Biosynthesis Biosynthesis Cell engineering Cell engineering Cytology Cytology Escherichia coli Escherichia coli Gene expression Gene expression Ketones Ketones Molecular dynamics Molecular dynamics Molecular orientation Molecular orientation
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| GB/T 7714 | Xu, Xinqi , Zhong, Jinchang , Su, Bingmei et al. Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning [J]. | International Journal of Biological Macromolecules , 2024 , 275 . |
| MLA | Xu, Xinqi et al. "Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning" . | International Journal of Biological Macromolecules 275 (2024) . |
| APA | Xu, Xinqi , Zhong, Jinchang , Su, Bingmei , Xu, Lian , Hong, Xiaokun , Lin, Juan . Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning . | International Journal of Biological Macromolecules , 2024 , 275 . |
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Hydroxytyrosol, a naturally occurring compound with antioxidant and antiviral activity, is widely applied in the cosmetic, food, and nutraceutical industries. The development of a biocatalytic approach for producing hydroxytyrosol from simple and readily accessible substrates remains a challenge. Here, we designed and implemented an effective biocatalytic cascade to obtain hydroxytyrosol from 3,4-dihydroxybenzaldehyde and l-threonine via a four-step enzymatic cascade composed of seven enzymes. To prevent cross-reactions and protein expression burden caused by multiple enzymes expressed in a single cell, the designed enzymatic cascade was divided into two modules and catalyzed in a stepwise manner. The first module (FM) assisted the assembly of 3,4-dihydroxybenzaldehyde and l-threonine into (2S,3R)-2-amino-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoic acid, and the second module (SM) entailed converting (2S,3R)-2-amino-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoic acid into hydroxytyrosol. Each module was cloned into Escherichia coli BL21 (DE3) and engineered in parallel by fine-tuning enzyme expression, resulting in two engineered whole-cell catalyst modules, BL21(FM01) and BL21(SM13), capable of converting 30 mM 3,4-dihydroxybenzaldehyde to 28.7 mM hydroxytyrosol with a high space-time yield (0.88 g/L/h). To summarize, the current study proposes a simple and effective approach for biosynthesizing hydroxytyrosol from low-cost substrates and thus has great potential for industrial applications.
Keyword ：
Biocatalytic cascade Biocatalytic cascade hydroxytyrosol hydroxytyrosol stepwisecatalysis stepwisecatalysis
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| GB/T 7714 | Liu, Wen-Kai , Su, Bing-Mei , Xu, Xin-Qi et al. Multienzymatic Cascade for Synthesis of Hydroxytyrosol via Two-Stage Biocatalysis [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (27) : 15293-15300 . |
| MLA | Liu, Wen-Kai et al. "Multienzymatic Cascade for Synthesis of Hydroxytyrosol via Two-Stage Biocatalysis" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 27 (2024) : 15293-15300 . |
| APA | Liu, Wen-Kai , Su, Bing-Mei , Xu, Xin-Qi , Xu, Lian , Lin, Juan . Multienzymatic Cascade for Synthesis of Hydroxytyrosol via Two-Stage Biocatalysis . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (27) , 15293-15300 . |
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Enantiopure 1,2-diols are widely used in the production of pharmaceuticals, cosmetics, and functional materials as essential building blocks or bioactive compounds. Nevertheless, developing a mild, efficient and environmentally friendly biocatalytic route for manufacturing enantiopure 1,2-diols from simple substrate remains a challenge. Here, we designed and realized a step-wise biocatalytic cascade to access chiral 1,2-diols starting from aromatic aldehyde and formaldehyde enabled by a newly mined benzaldehyde lyase from Sphingobium sp. combined with a pair of tailored-made short-chain dehydrogenase/reductase from Pseudomonas monteilii (PmSDR-MuR and PmSDR-MuS) capable of producing (R)- and (S)-1-phenylethane-1,2-diol with 99% ee. The planned biocatalytic cascade could synthesize a series of enantiopure 1,2-diols with a broad scope (16 samples), excellent conversions (94%-99%), and outstanding enantioselectivity (up to 99% ee), making it an effective technique for producing chiral 1,2-diols in a more environmentally friendly and sustainable manner. A step-wise biocatalytic cascade to access chiral 1,2-diols starting from aromatic aldehyde and formaldehyde enabled by a newly mined ThDP-dependent benzaldehyde lyase from Sphingobium sp. combined with a pair of tailored-made short-chain dehydrogenase/reductase from Pseudomonas monteilii (PmSDR-MuR and PmSDR-MuS). image
Keyword ：
benzaldehyde lyase benzaldehyde lyase chiral 1,2-diols chiral 1,2-diols rational design rational design short-chain dehydrogenase/reductase short-chain dehydrogenase/reductase
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| GB/T 7714 | Ren, Xiu-Xin , Su, Bing-Mei , Xu, Xin-Qi et al. Rational design of short-chain dehydrogenase/reductase for enantio-complementary synthesis of chiral 1,2-diols by successive hydroxymethylation and reduction of aldehydes [J]. | BIOTECHNOLOGY AND BIOENGINEERING , 2024 , 121 (12) : 3796-3807 . |
| MLA | Ren, Xiu-Xin et al. "Rational design of short-chain dehydrogenase/reductase for enantio-complementary synthesis of chiral 1,2-diols by successive hydroxymethylation and reduction of aldehydes" . | BIOTECHNOLOGY AND BIOENGINEERING 121 . 12 (2024) : 3796-3807 . |
| APA | Ren, Xiu-Xin , Su, Bing-Mei , Xu, Xin-Qi , Xu, Lian , Lin, Juan . Rational design of short-chain dehydrogenase/reductase for enantio-complementary synthesis of chiral 1,2-diols by successive hydroxymethylation and reduction of aldehydes . | BIOTECHNOLOGY AND BIOENGINEERING , 2024 , 121 (12) , 3796-3807 . |
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Norepinephrine, a kind of beta-adrenergic receptor agonist, is commonly used for treating shocks and hypotension caused by a variety of symptoms. The development of a straightforward, efficient and environmentally friendly biocatalytic route for manufacturing norepinephrine remains a challenge. Here, we designed and realized an artificial biocatalytic cascade to access norepinephrine starting from 3, 4-dihydroxybenzaldehyde and L-threonine mediated by a tailored-made L-threonine transaldolase PsLTTA-Mu1 and a newly screened tyrosine decarboxylase ErTDC. To overcome the imbalance of multi-enzymes in a single cell, engineering of PsLTTA for improved activity and fine-tuning expression mode of multi-enzymes in single E.coli cells were combined, leading to a robust whole cell biocatalyst ES07 that could produce 100 mM norepinephrine with 99% conversion, delivering a highest time-space yield (3.38 g/L/h) ever reported. To summarized, the current study proposed an effective biocatalytic approach for the synthesis of norepinephrine from low-cost substrates, paving the way for industrial applications of enzymatic norepinephrine production.
Keyword ：
Biocatalytic cascade Biocatalytic cascade L-threonine transaldolase L-threonine transaldolase Tyrosine decarboxylase Tyrosine decarboxylase
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| GB/T 7714 | Xu, Lian , Shen, Jun-Jiang , Wu, Ming et al. An artificial biocatalytic cascade for efficient synthesis of norepinephrine by combination of engineered L-threonine transaldolase with multi-enzyme expression fine-tuning [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 265 . |
| MLA | Xu, Lian et al. "An artificial biocatalytic cascade for efficient synthesis of norepinephrine by combination of engineered L-threonine transaldolase with multi-enzyme expression fine-tuning" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 265 (2024) . |
| APA | Xu, Lian , Shen, Jun-Jiang , Wu, Ming , Su, Bing-Mei , Xu, Xin-Qi , Lin, Juan . An artificial biocatalytic cascade for efficient synthesis of norepinephrine by combination of engineered L-threonine transaldolase with multi-enzyme expression fine-tuning . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 265 . |
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Dehydroepiandrosterone (DHEA) is an important neurosteroid hormone to keep human hormonal balance and reproductive health. However, DHEA was always produced with impurities either by chemical or biological method and required high-cost purification before the medical use. To address this issue, a novel chemo-enzymatic process was proposed and implemented to produce DHEA. An acetoxylated derivate of 4-androstene-3,17-dione (4-AD) was generated by chemical reaction and converted into DHEA by an enzyme cascade reaction combining a hydrolysis reaction with a reduction reaction. The hydrolysis reaction was catalyzed by a com-mercial esterase Z03 while the reduction reaction was catalyzed by E. coli cells co-expressing a 3 beta-hydroxysteroid dehydrogenase SfSDR and a glucose dehydrogenase BtGDH. After the condition optimization, DHEA was syn-thesized at a 100 mL scale under 100 mM of substrate loading and purified as white powder with the highest space-time yield (4.80 g/L/h) and purity (99 %) in the biosynthesis of DHEA. The successful attempt in this study provides a new approach for green synthesis of highly purified DHEA in the pharmaceutical industry.
Keyword ：
3?-hydroxysteroid dehydrogenase 3?-hydroxysteroid dehydrogenase Chemoenzymatic process Chemoenzymatic process Dehydroepiandrosterone Dehydroepiandrosterone Enzyme cascade Enzyme cascade Hydrolysis Hydrolysis
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| GB/T 7714 | Su, Bing-Mei , Shi, Yi-Bing , Lin, Wei et al. A chemoenzymatic process for preparation of highly purified dehydroepiandrosterone in high space-time yield [J]. | BIOORGANIC CHEMISTRY , 2023 , 133 . |
| MLA | Su, Bing-Mei et al. "A chemoenzymatic process for preparation of highly purified dehydroepiandrosterone in high space-time yield" . | BIOORGANIC CHEMISTRY 133 (2023) . |
| APA | Su, Bing-Mei , Shi, Yi-Bing , Lin, Wei , Xu, Lian , Xu, Xin-Qi , Lin, Juan . A chemoenzymatic process for preparation of highly purified dehydroepiandrosterone in high space-time yield . | BIOORGANIC CHEMISTRY , 2023 , 133 . |
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Laccase is an efficient biocatalyst for oxidative polymerization of organic substrates. However, cost of enzyme preparation, low stability and residual protein diminish the efficiency of laccase mediated polymerization. In this work, a series of silicon dioxide coated ferroferric oxide magnetic nanoparticles were modified by different functional groups including gamma-methacryloxypropyltrimethoxy, succinic anhydride, glutaraldehyde and poly-ethylene imine. Infrared spectra indicated the magnetic carriers have been successfully modified. Vibrating sample magnetometer (VSM) analysis revealed that all of these carriers showed high magnetic responsiveness after the surface functionalization. Laccase from Cerrena sp. HYB07 was then respectively immobilized covalently on these functionalized magnetic carriers. All the immobilized laccases displayed higher thermostability than free laccase and glutaraldehyde functionalized support (named FSNG) immobilized laccase showed better performance. These immobilized laccases all showed higher efficiency than free laccase for oxidative polymerization of catechol and hydroquinone. The immobilized laccases could be separated from the water insoluble polymerization products. The polymerization product of hydroquinone by FSNG immobilized laccase showed the average polymerization degree of the poly(hydroquinone) was six (DP=6). This work provided a comprehensive exploration of laccase immobilization on magnetic carrier for catalyzing polymerization of phenols.
Keyword ：
Laccase immobilization Laccase immobilization Magnetic nanoparticle Magnetic nanoparticle Phenol polymerization Phenol polymerization
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| GB/T 7714 | Xu, Xinqi , Chen, Tianheng , Xu, Lian et al. Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols [J]. | ENZYME AND MICROBIAL TECHNOLOGY , 2023 , 172 . |
| MLA | Xu, Xinqi et al. "Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols" . | ENZYME AND MICROBIAL TECHNOLOGY 172 (2023) . |
| APA | Xu, Xinqi , Chen, Tianheng , Xu, Lian , Lin, Juan . Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols . | ENZYME AND MICROBIAL TECHNOLOGY , 2023 , 172 . |
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2'-deoxyguanosine is a key medicinal intermediate that could be used to synthesize anti-cancer drug and biomarker in type 2 diabetes. In this study, an enzymatic cascade using thymidine phosphorylase from Escherichia coli (EcTP) and purine nucleoside phosphorylase from Brevibacterium acetylicum (BaPNP) in a one-pot whole cell catalysis was proposed for the efficient synthesis of 2'-deoxyguanosine. BaPNP was semi-rationally designed to improve its activity, yielding the best triple variant BaPNP-Mu3 (E57A/T189S/L243I), with a 5.6-fold higher production of 2'-deoxyguanosine than that of wild-type BaPNP (BaPNP-Mu0). Molecular dynamics simulation revealed that the engineering of BaPNP-Mu3 resulted in a larger and more flexible substrate entrance channel, which might contribute to its catalytic efficiency. Furthermore, by coordinating the expression of BaPNP-Mu3 and EcTP, a robust whole cell catalyst W05 was created, capable of producing 14.8 mM 2'-deoxyguanosine (74.0% conversion rate) with a high time-space yield (1.32 g/L/h) and therefore being very competitive for industrial applications.
Keyword ：
2'-deoxyguanosine 2'-deoxyguanosine Enzymatic cascade Enzymatic cascade Semi-rational design Semi-rational design Whole cell catalysis Whole cell catalysis
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| GB/T 7714 | Xu, Lian , Li, Hui-Min , Lin, Juan . Efficient synthesis of 2'-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum [J]. | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2023 , 39 (10) . |
| MLA | Xu, Lian et al. "Efficient synthesis of 2'-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum" . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 39 . 10 (2023) . |
| APA | Xu, Lian , Li, Hui-Min , Lin, Juan . Efficient synthesis of 2'-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2023 , 39 (10) . |
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本发明属于生物工程技术领域，具体涉及一种3β‑羟基甾体脱氢酶SfSDR及其在制备去氢表雄酮中的应用。本发明将3β‑羟基甾体脱氢酶SfSDR与葡萄糖脱氢酶BtGDH在大肠埃希氏菌中共表达，并以共表达工程菌的静息细胞作为生物催化剂协同酯酶Z03共同催化3‑乙酰氧基‑△3, 5‑雄甾二烯‑17‑酮合成去氢表雄酮。该生物催化剂具有较高的催化活性、区域选择性和立体选择性，可以在6 h以内完全转化32.8 g/L的3‑乙酰氧基‑△3, 5‑雄甾二烯‑17‑酮生成目标产物去氢表雄酮，无需添加有机溶剂且无副产物产生，经过分离纯化，产物回收率高达95%以上，说明该生物催化剂是去氢表雄酮绿色合成的高效催化剂。
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| GB/T 7714 | 林娟 , 苏冰梅 , 师艺冰 et al. 一种3β-羟基甾体脱氢酶及其在制备去氢表雄酮中的应用 : CN202211028698.9[P]. | 2022-08-25 00:00:00 . |
| MLA | 林娟 et al. "一种3β-羟基甾体脱氢酶及其在制备去氢表雄酮中的应用" : CN202211028698.9. | 2022-08-25 00:00:00 . |
| APA | 林娟 , 苏冰梅 , 师艺冰 , 许炼 , 许鑫琦 . 一种3β-羟基甾体脱氢酶及其在制备去氢表雄酮中的应用 : CN202211028698.9. | 2022-08-25 00:00:00 . |
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