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学者姓名:赖汉江

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Experimental Study on Multiscale Engineering Properties of EICP Combined with Xanthan Gum Solidified Sand SCIE
期刊论文 | 2024 , 36 (6) | JOURNAL OF MATERIALS IN CIVIL ENGINEERING
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Abstract :

In this study, enzyme-induced carbonate precipitation (EICP) combined with xanthan gum curing technology was used to improve the engineering properties of standard sand, and the curing effect of EICP combined with different xanthan gum contents was studied by macroscopic tests such as unconfined compressive strength, direct shear, permeability, calcium carbonate content, and microscopic tests such as scanning electron microscope and nuclear magnetic resonance. The results show that the unconfined compressive strength, shear strength, cohesion and internal friction angle of EICP combined with xanthan gum solidified sand increases with the increase of xanthan gum content and reaches the maximum value at the content of 2%, in which the increase of unconfined compressive strength, shear strength, and cohesion is significant; further, the increase of internal friction angle is small. The permeability coefficient of EICP combined with xanthan gum solidified sand decreases with the increase of xanthan gum content, and the permeability coefficient of 2% xanthan gum is only 65.4% that of pure EICP treatment. The incorporation of xanthan gum promotes the deposition of calcium carbonate, increases the viscosity of the reaction solution, and produces colloidal encapsulation and bonding effect on the sand particles. In addition, the incorporation of xanthan gum effectively reduces the porosity of solidified sand and greatly reduces the proportion of large pores and medium pores by changing the pore size, which greatly improves the pore structure.

Keyword :

Enzyme-induced carbonate precipitation (EICP) Enzyme-induced carbonate precipitation (EICP) Multiscale engineering properties Multiscale engineering properties Soybean urease Soybean urease Standard sand Standard sand Xanthan gum Xanthan gum

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GB/T 7714 Cui, Meng , Xiong, Huihui , Zheng, Junjie et al. Experimental Study on Multiscale Engineering Properties of EICP Combined with Xanthan Gum Solidified Sand [J]. | JOURNAL OF MATERIALS IN CIVIL ENGINEERING , 2024 , 36 (6) .
MLA Cui, Meng et al. "Experimental Study on Multiscale Engineering Properties of EICP Combined with Xanthan Gum Solidified Sand" . | JOURNAL OF MATERIALS IN CIVIL ENGINEERING 36 . 6 (2024) .
APA Cui, Meng , Xiong, Huihui , Zheng, Junjie , Cui, Mingjuan , Lv, Suying , Lai, Hanjiang . Experimental Study on Multiscale Engineering Properties of EICP Combined with Xanthan Gum Solidified Sand . | JOURNAL OF MATERIALS IN CIVIL ENGINEERING , 2024 , 36 (6) .
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Optimization of one-phase-low-pH enzyme-induced carbonate precipitation method for soil improvement SCIE
期刊论文 | 2024 , 19 (3) , 1611-1625 | ACTA GEOTECHNICA
WoS CC Cited Count: 5
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Abstract :

There are two major challenges for the use of enzyme-induced carbonate precipitation (EICP)-based soil improvement method: cost and treatment effect. Optimizing the parameters of the treatment solution is one way to enhance the treatment effect and/or reduce the treatment cost. In this study, three key factors: the initial pH (i.e. pH0) of the enzyme solution used to prepare the treatment solution (i.e. the mixture of enzyme solution, CaCl2 and urea), the urease activity of the treatment solution and the concentration of cementation solution (i.e. CaCl2 and urea) are investigated. Crude soybean enzyme solution and the one-phase-low-pH injection method are adopted for the treatment of sand. The results show that the pH0 of the enzyme solution affects the urease activity of enzyme and thus the urease activity of the prepared treatment solution. It is discovered in this paper that there is a threshold pH value for the treatment solution. Only when the pH of the treatment solution is higher than the threshold pH value, calcium ions convert completely into calcium carbonate. There is also a threshold urease activity which is affected by the concentration of cementation solution, CCS. The optimal CCS is 1.0 M. When the CCS is higher than 1.75 M, the urease activity of soybean enzyme solution would be completely lost. These findings are important in guiding the application of EICP treatment using the crude soybean enzyme in real soil improvement projects.

Keyword :

Crude soybean enzyme Crude soybean enzyme Enzyme-induced carbonate precipitation Enzyme-induced carbonate precipitation Influencing factors Influencing factors Soil improvement Soil improvement

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GB/T 7714 Cui, Ming-Juan , Chu, Jian , Lai, Han-Jiang . Optimization of one-phase-low-pH enzyme-induced carbonate precipitation method for soil improvement [J]. | ACTA GEOTECHNICA , 2024 , 19 (3) : 1611-1625 .
MLA Cui, Ming-Juan et al. "Optimization of one-phase-low-pH enzyme-induced carbonate precipitation method for soil improvement" . | ACTA GEOTECHNICA 19 . 3 (2024) : 1611-1625 .
APA Cui, Ming-Juan , Chu, Jian , Lai, Han-Jiang . Optimization of one-phase-low-pH enzyme-induced carbonate precipitation method for soil improvement . | ACTA GEOTECHNICA , 2024 , 19 (3) , 1611-1625 .
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Biomineralization of soil with crude soybean urease using different calcium salts Scopus CSCD
期刊论文 | 2024 , 16 (5) , 1788-1798 | Journal of Rock Mechanics and Geotechnical Engineering
SCOPUS Cited Count: 2
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Calcium salt is an important contributing factor for calcium-based biomineralization. To study the effect of calcium salt on soil biomineralization using crude soybean urease, the calcium salts, including the calcium chloride (CaCl2), calcium acetate ((CH3COO)2Ca) and calcium nitrate (Ca(NO3)2), were used to prepare the biotreatment solution to carry out the biomineralization tests in this paper. Two series of biomineralization tests in solution and sand column, respectively, were conducted. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were performed to determine the microscopic characteristics of the precipitated calcium carbonate (CaCO3) crystals. The experimental results indicate that the biomineralization effect is the best for the CaCl2 case, followed by (CH3COO)2Ca, and worst for Ca(NO3)2 under the test conditions of this study (i.e. 1 mol/L of calcium salt-urea). The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves: (1) inhibition of urease activity, and (2) influence on the crystal size and morphology of CaCO3. Besides Ca2+, the anions in solution can inhibit the activity of crude soybean urease, and NO3− has a stronger inhibitory effect on the urease activity compared with both CH3COO− and Cl−. The co-inhibition of Ca2+ and NO3− on the activity of urease is the key reason for the worst biomineralization of the Ca(NO3)2 case in this study. The difference in biomineralization between the CaCl2 and (CH3COO)2Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO3. © 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences

Keyword :

Biomineralization Biomineralization Calcium salt Calcium salt Crude soybean urease Crude soybean urease Influence mechanism Influence mechanism

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GB/T 7714 Weng, Y. , Zheng, J. , Lai, H. et al. Biomineralization of soil with crude soybean urease using different calcium salts [J]. | Journal of Rock Mechanics and Geotechnical Engineering , 2024 , 16 (5) : 1788-1798 .
MLA Weng, Y. et al. "Biomineralization of soil with crude soybean urease using different calcium salts" . | Journal of Rock Mechanics and Geotechnical Engineering 16 . 5 (2024) : 1788-1798 .
APA Weng, Y. , Zheng, J. , Lai, H. , Cui, M. , Ding, X. . Biomineralization of soil with crude soybean urease using different calcium salts . | Journal of Rock Mechanics and Geotechnical Engineering , 2024 , 16 (5) , 1788-1798 .
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Seawater-based soybean urease for calcareous sand biomineralization SCIE
期刊论文 | 2024 , 19 (10) , 6643-6659 | ACTA GEOTECHNICA
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Abstract :

In tropical islands, calcareous sand with poor engineering properties usually needs to be treated before it can be used as building materials. Considering the scarcity of freshwater in these areas, this study proposes seawater-based enzyme induced carbonate precipitation (EICP) technology to enhance the properties of calcareous sand. It is to induce calcium carbonate crystals to bond calcareous sand particles together using the seawater-based crude soybean enzyme and cementation solution (i.e., urea and calcium chloride). In this study, the crude soybean urease extraction test was firstly carried out using seawater and it was also investigated what components of seawater had a greater effect on the soybean urease extraction. Afterwards, the solution test was conducted to explore the ability of the extracted urease in inducing calcium carbonate through analyzing the variation of concentration of calcium ions and pH of the solution. Finally, the biocementation effect of EICP treated calcareous sand using the seawater extracted urease solution was evaluated by the unconfined compressive strength (quc) and microscopic analysis. Test results show that the turbidity of the seawater-extracted soybean urease solution can be reduced by 66.7% compared to that of deionised water extracted urease, with only a slight reduction in urease activity. Among all the components of seawater, NaCl, MgCl2, CaCl2, NaHCO3 and KBr can significantly reduce the turbidity of soybean urease solution. The lower turbidity can effectively avoid bioclogging and contribute to the homogeneity of the EICP-treated calcareous sands, and thus improve the biomineralization efficiency and strength enhancement. Seawater-based EICP treatment will be a great promising technology in freshwater-scarce tropical islands, because it can directly use seawater for biomineralization treatment of calcareous sand, and meanwhile effectively avoid local clogging of biocementation.

Keyword :

Biomineralization Biomineralization Calcareous sand Calcareous sand Enzyme induced carbonate precipitation (EICP) Enzyme induced carbonate precipitation (EICP) Seawater Seawater Soybean urease extraction Soybean urease extraction

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GB/T 7714 Cui, Ming-Juan , Zhou, Jia-Ni , Lai, Han-Jiang et al. Seawater-based soybean urease for calcareous sand biomineralization [J]. | ACTA GEOTECHNICA , 2024 , 19 (10) : 6643-6659 .
MLA Cui, Ming-Juan et al. "Seawater-based soybean urease for calcareous sand biomineralization" . | ACTA GEOTECHNICA 19 . 10 (2024) : 6643-6659 .
APA Cui, Ming-Juan , Zhou, Jia-Ni , Lai, Han-Jiang , Zheng, Jun-Jie , Huang, Ming , Zhang, Zhi-Chao . Seawater-based soybean urease for calcareous sand biomineralization . | ACTA GEOTECHNICA , 2024 , 19 (10) , 6643-6659 .
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Biomineralization of soil with crude soybean urease using different calcium salts CSCD
期刊论文 | 2024 , 16 (5) , 1788-1798 | 岩石力学与岩土工程学报(英文版)
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Abstract :

Calcium salt is an important contributing factor for calcium-based biomineralization.To study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chloride(CaCl2),calcium acetate((CH3COO)2Ca)and calcium nitrate(Ca(NO3)2),were used to prepare the biotreatment solution to carry out the biomineralization tests in this paper.Two series of biomineralization tests in solution and sand column,respectively,were conducted.Scanning electron microscopy(SEM)and X-ray diffraction(XRD)were performed to determine the microscopic charac-teristics of the precipitated calcium carbonate(CaCO3)crystals.The experimental results indicate that the biomineralization effect is the best for the CaCl2 case,followed by(CH3COO)2Ca,and worst for Ca(NO3)2 under the test conditions of this study(i.e.1 mol/L of calcium salt-urea).The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves:(1)inhibition of urease activity,and(2)influence on the crystal size and morphology of CaCO3.Besides Ca2+,the anions in solution can inhibit the activity of crude soybean urease,and NO3 has a stronger inhibitory effect on the urease activity compared with both CH3COO-and Cl-.The co-inhibition of Ca2+and NO3 on the activity of urease is the key reason for the worst biomineralization of the Ca(NO3)2 case in this study.The dif-ference in biomineralization between the CaCl2 and(CH3COO)2Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO3.

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GB/T 7714 Yajie Weng , Junjie Zheng , Hanjiang Lai et al. Biomineralization of soil with crude soybean urease using different calcium salts [J]. | 岩石力学与岩土工程学报(英文版) , 2024 , 16 (5) : 1788-1798 .
MLA Yajie Weng et al. "Biomineralization of soil with crude soybean urease using different calcium salts" . | 岩石力学与岩土工程学报(英文版) 16 . 5 (2024) : 1788-1798 .
APA Yajie Weng , Junjie Zheng , Hanjiang Lai , Mingjuan Cui , Xingzhi Ding . Biomineralization of soil with crude soybean urease using different calcium salts . | 岩石力学与岩土工程学报(英文版) , 2024 , 16 (5) , 1788-1798 .
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Inorganic flocculant-based soybean urease extraction and its effect on biomineralization SCIE
期刊论文 | 2024 , 460 | JOURNAL OF CLEANER PRODUCTION
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Enzyme induced carbonate precipitation (EICP) based on self -extracted crude soybean urease solution (CSUS) is a promising method for soil improvement. However, the deionized water -extracted CSUS usually contains a large amount of impurities that easily lead to bioclogging during the biogrouting process, resulting in a nonuniform biomineralization effect. In this study, a purification method using inorganic flocculants is proposed to extract CSUS with relatively high purity and urease activity (UA) for EICP method. Seven commonly used inorganic flocculants were adopted in this study, including KAl(SO4)2 center dot 12H2O, AlCl3 center dot 6H2O, Al2(SO4)3 center dot 18H2O, Fe2(SO4)3, poly aluminum chloride, poly ferric sulfate, and poly aluminum ferric chloride. Three sets of tests, including CSUS extraction tests, solution tests, and sand column treatment tests, were conducted to investigate the feasibility and validity of this purification method. The test results show that inorganic flocculants could effectively reduce the turbidity of the extracted CSUS, avoid the bioclogging during biogrouting, and thus improve the biomineralization effect of the CSUS-based EICP method. Compared with deionized water -based CSUS, at least 60% of the impurities in CSUS can be removed at optimal flocculant contents when the soybean powder content is 100 g/L. On the other hand, the flocculants would also cause a reduction in the UA of the extracted CSUS. Considering the UA, turbidity, and biomineralization effect of the extracted CSUS, the optimal inorganic flocculants and their contents are recommended to be 3.0 g/L for KAl(SO4)2 center dot 12H2O, 2.0 g/L for AlCl3 center dot 6H2O, 2.5 g/L for Al2(SO4)3 center dot 18H2O under the tested conditions, respectively.

Keyword :

Biomineralization Biomineralization Enzyme induced carbonate precipitation (EICP) Enzyme induced carbonate precipitation (EICP) Inorganic flocculant Inorganic flocculant Soybean urease Soybean urease Urease extraction Urease extraction

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GB/T 7714 Lai, Han-Jiang , Liu, Hui , Cui, Ming-Juan et al. Inorganic flocculant-based soybean urease extraction and its effect on biomineralization [J]. | JOURNAL OF CLEANER PRODUCTION , 2024 , 460 .
MLA Lai, Han-Jiang et al. "Inorganic flocculant-based soybean urease extraction and its effect on biomineralization" . | JOURNAL OF CLEANER PRODUCTION 460 (2024) .
APA Lai, Han-Jiang , Liu, Hui , Cui, Ming-Juan , Zheng, Jun-Jie , Chen, Zhi-Bo , Zhang, Zhi-Chao . Inorganic flocculant-based soybean urease extraction and its effect on biomineralization . | JOURNAL OF CLEANER PRODUCTION , 2024 , 460 .
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Factors affecting the effectiveness of biocementation of soil Scopus
期刊论文 | 2024 , 2 (3) | Biogeotechnics
SCOPUS Cited Count: 2
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Microbially or enzyme induced carbonate precipitation has emerged to be a new type of soil improvement method. However, it appears that the biocementation process is affected by many factors and a common understanding on the control factors on the biocement effect has not been reached. This paper attempts to identify the main factors that controlling the MICP or EICP effect through an in-depth discussion on the fundamentals of biocementation process. Similar to other cemented granular materials, biocemented soil is a structural soil composite consisting of soil skeleton and biocement force chain or biocement network. The strength and stiffness of the biocemented soil is controlled by the reinforcement effect of the biocement network on the soil skeleton or the interplay of the soil skeleton and precipitates. The contribution of the strength by soil skeleton is affected by the soil types and soil properties, while the contribution of the precipitates is through the distribution of the biocement network and the properties of the precipitates. © 2024

Keyword :

Biocementation Biocementation Influencing factor Influencing factor Mechanism Mechanism Strength enhancement Strength enhancement

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GB/T 7714 Lai, H. , Ding, X. , Cui, M. et al. Factors affecting the effectiveness of biocementation of soil [J]. | Biogeotechnics , 2024 , 2 (3) .
MLA Lai, H. et al. "Factors affecting the effectiveness of biocementation of soil" . | Biogeotechnics 2 . 3 (2024) .
APA Lai, H. , Ding, X. , Cui, M. , Zheng, J. , Chu, J. , Chen, Z. . Factors affecting the effectiveness of biocementation of soil . | Biogeotechnics , 2024 , 2 (3) .
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Stress-Dilatancy Behavior of Biocementation-Enhanced Geogrid-Reinforced Sand SCIE
期刊论文 | 2023 , 23 (5) | INTERNATIONAL JOURNAL OF GEOMECHANICS
WoS CC Cited Count: 4
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Abstract :

The performance of geogrid reinforcement is largely influenced by the interlocking effect between the geogrid and soil. To enhance the performance of geogrid reinforcement in sand, a novel biocementation-enhanced geogrid reinforcement method was proposed. Biocementation could be carried out in situ via microbially-induced carbonate precipitation (MICP). To understand the mechanisms of the biocementation enhancement effect, the stress-dilatancy behavior of coarse sand (CS) that was treated with the biocementation-enhanced geogrid reinforcement method was studied through a series of triaxial tests. The test results showed that the interlocking effect between the geogrid and CS could be effectively improved when the CS around the geogrid was biocemented by the calcium carbonate (CaCO3) that was generated in situ through MICP. The stress-dilatancy behavior of geogrid-reinforced CS was significantly enhanced after biocementation. In addition, the interlocking effect that was provided by the geogrid could mitigate or even avoid brittle failure, restrain the further development of dilation, and enhance the mobilization of the friction strength (q(f)) of the biocemented coarse sand (Bio-CS) during shearing.

Keyword :

Biocementation Biocementation Coarse sand Coarse sand Geogrid reinforcement Geogrid reinforcement Interlocking effect Interlocking effect Stress-dilatancy behavior Stress-dilatancy behavior

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GB/T 7714 Lai, Han-Jiang , Cui, Ming-Juan , Chu, Jian . Stress-Dilatancy Behavior of Biocementation-Enhanced Geogrid-Reinforced Sand [J]. | INTERNATIONAL JOURNAL OF GEOMECHANICS , 2023 , 23 (5) .
MLA Lai, Han-Jiang et al. "Stress-Dilatancy Behavior of Biocementation-Enhanced Geogrid-Reinforced Sand" . | INTERNATIONAL JOURNAL OF GEOMECHANICS 23 . 5 (2023) .
APA Lai, Han-Jiang , Cui, Ming-Juan , Chu, Jian . Stress-Dilatancy Behavior of Biocementation-Enhanced Geogrid-Reinforced Sand . | INTERNATIONAL JOURNAL OF GEOMECHANICS , 2023 , 23 (5) .
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Application of Non-Reinforced Rigid Inclusion Columns as Foundation Support for Container Yard CPCI-S
期刊论文 | 2023 , 105-113 | GEO-CONGRESS 2023: FOUNDATIONS, RETAINING STRUCTURES, AND GEOSYNTHETICS
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Steel reinforced concrete piles have commonly been adopted as foundations to support container yards in Singapore. However, such a type of foundation causes great difficulties for demolition for future redevelopment. To overcome this problem, a new foundation type has been developed. Non-reinforced rigid inclusion columns together with T-beams are used to support a load transfer platform to provide bearing capacity and reduce settlements for the containers. The arching effect is created in the top sand fill to distribute the loads and control the deformation of the foundation. A conceptual design based on the new approach is proposed. An analytical method is established for the design of the proposed foundation. The key factors controlling the design are discussed. The performance of the proposed foundation is evaluated using 3D finite element analyses.

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GB/T 7714 Chen, Hao , Lai, Hanjiang , Wu, Shifan et al. Application of Non-Reinforced Rigid Inclusion Columns as Foundation Support for Container Yard [J]. | GEO-CONGRESS 2023: FOUNDATIONS, RETAINING STRUCTURES, AND GEOSYNTHETICS , 2023 : 105-113 .
MLA Chen, Hao et al. "Application of Non-Reinforced Rigid Inclusion Columns as Foundation Support for Container Yard" . | GEO-CONGRESS 2023: FOUNDATIONS, RETAINING STRUCTURES, AND GEOSYNTHETICS (2023) : 105-113 .
APA Chen, Hao , Lai, Hanjiang , Wu, Shifan , Lim, Stephen , Song, Tiancheng , Chu, Jian . Application of Non-Reinforced Rigid Inclusion Columns as Foundation Support for Container Yard . | GEO-CONGRESS 2023: FOUNDATIONS, RETAINING STRUCTURES, AND GEOSYNTHETICS , 2023 , 105-113 .
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Methods for Enhancing Uniformity of Biocementation Treatment in Sand CPCI-S
期刊论文 | 2023 , 339 , 361-369 | GEO-CONGRESS 2023: SOIL IMPROVEMENT, GEOENVIRONMENTAL, AND SUSTAINABILITY
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One of the difficulties for the application of microbial induced carbonate precipitation (MICP) or enzyme induced carbonate precipitation (EICP) for soil treatment is to ensure uniformity in the treatment across the whole soil sample or soil profile. Methods that can be adopted to improve the uniformity of MICP or EICP treatment are reviewed and compared. Experimental data are presented to demonstrate that a newly proposed one-phase-low-pH injection method enables better uniformity to be achieved among different methods. Other factors affecting the uniformity of treatment are discussed. Measures to be adopted to enhance the uniformity of MICP or EICP treatment in sand are suggested.

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GB/T 7714 Lai, Han-Jiang , Cui, Ming-Juan , Wu, Shi-Fan et al. Methods for Enhancing Uniformity of Biocementation Treatment in Sand [J]. | GEO-CONGRESS 2023: SOIL IMPROVEMENT, GEOENVIRONMENTAL, AND SUSTAINABILITY , 2023 , 339 : 361-369 .
MLA Lai, Han-Jiang et al. "Methods for Enhancing Uniformity of Biocementation Treatment in Sand" . | GEO-CONGRESS 2023: SOIL IMPROVEMENT, GEOENVIRONMENTAL, AND SUSTAINABILITY 339 (2023) : 361-369 .
APA Lai, Han-Jiang , Cui, Ming-Juan , Wu, Shi-Fan , Chu, Jian . Methods for Enhancing Uniformity of Biocementation Treatment in Sand . | GEO-CONGRESS 2023: SOIL IMPROVEMENT, GEOENVIRONMENTAL, AND SUSTAINABILITY , 2023 , 339 , 361-369 .
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