Compared　to　normal　cells,　cancer　cells　possess　elevated　levels　of　glutathione　(GSH)　and　are　characterized　by　a　more　acidic　microenvironment.　Taking　advantage　of　this　property,　a　GSH-sensitive　probe　Ir-dt,　and　a　pH-sensitive　probe　Ir-OH,　were　designed　and　synthesized　in　this　work.　Due　to　the　reducibility　of　GSH,　the　diketone　part　of　the　Ir-dt　ligand　was　first　changed　to　OH.　After　that,　OH　was　oxidized　to　COOH　by　oxygen　in　the　air　due　to　its　instability,　resulting　in　enhanced　photoluminescence　(PL)　of　the　solution　after　the　reaction　with　GSH.　Ir-dt　had　good　specificity　in　response　to　GSH,　and　was　not　interfered　by　thiols　such　as　Hcy　and　Cys.　With　the　change　of　acid-base　environment,　the　OH　group　in　Ir-OH　easily　gained　or　lost　protons,　which　led　to　the　change　of　electron　cloud　distribution　in　the　molecule.　When　the　pH　of　the　solution　changed　from　acidic　to　alkaline,　the　PL　color　of　the　solution　shifted　progressively　from　red　to　green.　Based　on　the　changes　of　PL　wavelength　and　intensity　before　and　after　the　reaction,　a　ratio　PL　detection　method　for　GSH　and　pH　was　designed.　The　PL　of　their　mixed　solutions　was　green　when　the　GSH　content　was　low　and　the　pH　was　alkaline,　and　gradually　became　red　with　the　GSH　content　increases　and　the　pH　decreases.　After　the　verification　of　cell　experiments,　this　method　was　expected　to　realize　the　specific　detection　of　cancer　cells.　©　2025　Elsevier　Ltd