As　a　self-powered　device,　triboelectric　fiber-generators　(TEFGs)　have　garnered　considerable　attention　over　the　past　decade,　overcoming　the　limitations　of　conventional　power　sources.　Significantly,　while　both　structures　function,　the　aligned　nanofiber　film　demonstrably　outperforms　the　random　nanofiber　film　in　triboelectric　output　performance.　Hence,　a　triboelectric　all-nanofiber　generator　with　an　aligned　structure　is　investigated,　consisting　of　the　co-electrospun　polyacrylonitrile　(PAN)/thermoplastic　polyurethane　(TPU)　nanofiber　positive　triboelectric　layer　and　polyvinylidene　fluoride　(PVDF)/polydimethylsiloxane　(PDMS)　nanofiber　negative　triboelectric　layer.　After　the　optimization　of　structure,　triboelectric　fiber-generators　with　aligned　(A-TEFG)　exhibited　peak-to-peak　voltage,　current　output,　and　transient　power　density　values　of　55　V,　0.8　μA,　and　33.6　mW/m2,　respectively,　which　could　efficiently　harvest　mechanical　energy　to　power　light-emitting　diodes　(LEDs).　Furthermore,　A-TEFG　was　used　as　self-powered　sensors　for　human　movement　monitoring　and　transmitting　encrypted　information　followed　Morse　code　principles.　Based　on　the　simple　and　scalable　fabrication　methods,　A-TEFG　makes　a　promising　candidate　to　developing　practical,　flexible,　and　self-powered　wearable　electronic　devices.　©　2025