The　paper　described　the　synthesis　a　series　of　novel　long-chain　quaternary　ammonium　salts　based　on　ferrocene　carboxylic　acid.　The　structures　of　these　salts　were　characterized　through　1H　NMR,　13C　NMR,　HRMS　(ESI),　and　UV–Vis.　Their　electrochemical　behavior　was　evaluated　through　cyclic　voltammetry,　and　the　surface　properties　were　assessed　by　measuring　surface　tension　and　conductivity.　Additionally,　the　antimicrobial　effects　of　the　target　molecules　were　tested　against　both　Gram-positive　bacteria　(S.　aureus)　and　Gram-negative　bacteria　(Escherichia　coli).　The　activity　results　indicated　that　increasing　the　length　of　the　alkyl　chain　in　the　compounds　significantly　decreased　the　critical　micelle　concentration　and　greatly　enhanced　the　antimicrobial　efficacy.　Furthermore,　the　introduction　of　ferrocene　improved　the　antibacterial　activity　of　the　compounds　against　S.　aureus.　Structurally,　the　Gemini-type　compound　FcCnNNCnFc,　featuring　a　single-chain　FcCnN　linked　by　a　hexyl　group,　exhibited　a　pronounced　decrease　in　critical　micelle　concentration　and　a　significant　improvement　in　antimicrobial　activity.　These　ferrocene-containing　quaternary　ammonium　salt　compounds　proposed　showed　promising　potential　alternatives　for　antimicrobial　agents　or　surfactants　used　in　industrial　applications.　©　2025