Four　Gram-stain-negative　bacterial　strains　which　can　metabolize　alkanes　and　polycyclic　aromatic　hydrocarbons　were　isolated.　Strains　R06ZXJ2T　and　R09ZXJ6　were　isolated　from　sediments　collected　in　the　Arctic　Ocean.　Strains　DY715-9T　and　DY716-13-1　were　isolated　from　surface　sediment　samples　collected　by　the　deep-sea　TV　grab　in　the　southwestern　Indian　Ocean.　Phylog-enomic　analyses　based　on　16S　rRNA　gene　sequences,　average　nt　identity　(ANI)　and　digital　DNA–DNA　hybridization　(dDDH)　revealed　that　the　strains　formed　two　distinct　lineages　within　the　genus　Vreelandella.　Their　closest　relative　was　Vreelandella　maris　QX-1T,　with　ANI　values　of　94.35%　(R06ZXJ2T)　and　95.54%　(DY715-9T)　and　dDDH　values　of　57.00%　and　61.80%,　respec-tively.　The　pairwise　ANI　(95.47%)　and　dDDH　(60.70%)　between　the　two　novel　strains　further　supported　their　status　as　separate　species.　Phenotypic　and　chemotaxonomic　analyses　distinguished　them　from　known　Vreelandella　species　through　differences　in　colony　morphology,　hydrocarbon　degradation　profiles　and　polar　lipid　compositions.　The　strains　grew　optimally　at　28　°C　and　pH　7.0　and　can　degrade　alkanes　and　polycyclic　aromatic　hydrocarbons.　Based　on　these　results,　we　propose　two　novel　species:　Vreelandella　arctica　sp.　nov.　(type　strain　R06ZXJ2T=MCCC　1A08467T=KCTC　92631T)　and　Vreelandella　indica　sp.　nov.　(type　strain　DY715-9T=MCCC　1A12469T=KCTC　92628T).　©　2025　The　Authors.