There　are　multiple　lead　species,　including　inorganic　and　organic　lead,　that　may　simultaneously　be　present　in　marine　organisms.　Thus,　it　is　crucial　to　detect　lead　species　in　seafood　for　scientifically　evaluating　the　health　risk　of　lead　in　seafood.　We　herein　developed　a　cost-effective　short-column　ion　chromatography-inductively　coupled　plasma　mass　spectrometry　(IC-ICP-MS)　method　and　a　broadly　applicable　microwave-assisted　extraction　method　for　the　speciation　analysis　of　lead　in　various　types　of　seafood,　including　different　species　of　seaweed,　fish　and　shellfish.　The　short-column　IC-ICP-MS　method　utilized　low-cost　cation　guard　columns　as　separation　columns,　offering　high　sensitivity,　shorter　analysis　time　and　lower　analysis　costs.　The　microwave-assisted　extraction　method　is　widely　applicable;　it　can　be　used　to　extract　lead　species　from　various　types　of　seafood　with　an　extraction　efficiency　＞90%,　while　keeping　lead　species　unaltered.　In　particular,　the　seafood　extract　obtained　with　the　extraction　method　can　be　directly　injected　into　the　short-column　IC-ICP-MS　for　the　detection　of　lead　species　without　extra　pretreatment.　The　developed　extraction　and　IC-ICP-MS　method　has　been　successfully　used　to　detect　Pb2+,　tri-methyl　lead　(TML)　and　tri-ethyl　lead　(TEL)　in　various　species　of　seaweed,　fish　and　shellfish　with　a　recovery　of　94-105%,　a　relative　standard　deviation　(RSD,　n　=　5)　＜6%　and　a　method　detection　limit　of　0.60-0.72　ng　Pb　g(-1)　dried　seafood.　The　success　of　this　study　offers　a　reliable　and　universal　approach　for　objectively　assessing　the　health　risks　associated　with　lead　originating　from　seafood.