The　rise　of　antibiotic-resistant　bacteria　poses　a　serious　global　health　threat,　highlighting　the　urgent　need　for　novel　strategies　beyond　conventional　antibiotic　therapies.　This　study　explores　the　potential　of　microbe-imprinted　polymers　(MIPs)　as　innovative,　pathogen-specific　affinity　agents.　Utilizing　microbial　surface-initiated　polymerization,　MIPs　are　in-situ　synthesized　on　the　surface　of　target　microbes,　creating　flexible　heteropolymers　that　precisely　replicate　microbial　surface　structures.　This　method　exhibits　high　affinity　(KD　=　2.7x108　CFU/mL　for　E.　coli)　and　selectivity　at　the　strain　level.　MIPs　offer　significant　advantages　over　traditional　antibodies,　including　greater　stability,　cost-effectiveness,　and　a　broader　spectrum　of　binding　capabilities,　making　them　effective　for　identifying　and　targeting　various　microbial　strains,　including　unidentified　or　drug-resistant　variants.　Moreover,　their　favorable　biocompatibility　and　functional　resilience　in　diverse　environments　position　MIPs　as　promising　candidates　for　rapid　pathogen　detection　and　therapeutic　applications.　This　research　paves　the　way　for　advanced　biomimetic　materials　in　microbe-specific　diagnostics　and　combating　infections,　addressing　the　critical　need　for　effective　tools　in　antibiotic　resistance　surveillance.