Background:　Bulk-type　conductive　metal-organic　frameworks　(c-MOFs)　had　been　applied　to　modify　the　electrode　and　used　in　electrochemical　sensing　because　of　the　high　conductive　properties.　But　which　still　suffer　from　low　mass　permeability,　restricted　active　site　exposure,　and　poor　accessibility　due　to　the　coordination　saturation　at　metal　sites　in　the　bulk-type　c-MOF.　Recent　studies　have　demonstrated　that　transforming　bulk-type　MOFs　into　MOFs　nanosheets　(NSs)　can　maximize　the　exposure　of　active　sites　and　mass　transfer.　However,　c-MOF　NSs　have　rarely　been　applied　in　electrochemical　sensing.　Results:　This　study　presents　NSs　type　c-MOF　Cu3(benzenehexathiol)　(CuBHT),　synthesized　using　a　simple　sacrificial　template　method.　CuBHT　NSs　were　modified　onto　a　glassy　carbon　electrode　(GCE)　to　prepare　CuBHT　NSs/GCE,　which　was　then　applied　to　sense　the　model　target　ascorbic　acid　(AA),　the　system　exhibits　high　sensitivity　of　1.521　mA　mM−1　cm−2　and　a　wide　linear　range　of　1–789　μM,　low　detection　limit　of　0.46　μM.　The　sensitivity　is　1.90　times　higher　than　that　of　bulk-type　CuBHT　nanoparticles　(NPs)　modified　GCE,　which　can　be　attributed　to　the　CuBHT　NSs　having　more　exposed　Cu　sites　on　their　surfaces.　CuBHT　NSs/GCE　was　then　used　to　monitor　AA　levels　in　human　sweat　during　daily　activities　or　exercise,　and　the　results　indicated　high　reliability　compared　to　the　vitamin　C　ASA　kit　method.　Significance:　The　design　of　c-MOF　CuBHT　NSs/GCE　lead　to　better　performance　in　terms　of　sensitivity　and　low　detection　limit　in　AA　sensing　compared　to　bulk-type　nanoparticles.　The　AA　sensing　mechanism　based　on　CuBHT　was　investigated,　and　the　sensing　system　was　demonstrated　by　detecting　AA　in　sweat.　This　work　advances　both　the　fundamental　understanding　and　practical　applications　of　c-MOF　NSs　in　AA　sensing.　©　2025　Elsevier　B.V.