This　paper　provides　a　comprehensive　review　of　antimicrobial　peptides　(AMPs)　derived　from　Bacillus　spp.　The　classification　and　structure　of　Bacillus-derived　AMPs　encompass　a　diverse　range.　There　are　89　documented　Bacillus-derived　AMPs,　which　exhibit　varied　sources,　amino　acid　sequences,　and　molecular　structures.　These　AMPs　can　be　categorized　into　classes　I,　Ia,　IIa,　IIb,　IIc,　and　IId.　The　synthesis　pathway　of　the　AMPs　primarily　involves　either　ribosomally　synthesized　or　non-ribosomally　synthesized　approaches.　Additionally,　the　antimicrobial　activity　of　these　AMPs　is　versatile,　targeting　bacteria,　fungi,　and　viruses,　through　disrupting　intracellular　DNA　and　the　cell　wall　and　membrane,　as　well　as　modulating　immune　responses.　Moreover,　the　Bacillus-derived　AMPs　demonstrate　promising　application　in　the　pharmaceutical　industry,　environmental　protection,　food　preservation,　and　bio-control　in　agriculture.　The　commonly　employed　strategies　for　enhancing　the　production　of　Bacillus-derived　AMPs　involve　optimizing　cultivation　conditions,　implementing　systems　metabolic　engineering,　employing　genome　shuffling　techniques,　optimizing　promoters,　and　improving　expression　host　optimization.　This　review　can　provide　a　valuable　reference　for　comprehending　the　current　research　status　on　advancements　and　sustainable　production　of　Bacillus-derived　AMPs.　©　The　Author(s),　under　exclusive　licence　to　Springer　Science+Business　Media,　LLC,　part　of　Springer　Nature　2024.