TAC　(total　antioxidant　capacity)　is　one　of　the　most　significant　index　which　is　applied　to　reveal　the　oxidative　stress　condition　in　vitro　and　in　vivo　as　well　as　estimate　the　capability　of　antioxidant　food.　However,　the　features　of　complex　equipment　and　sample　preparation　limit　its　broader　development　vision.　Therefore,　it　is　essential　for　establishing　a　rapid,　convenient,　and　accurate　way　to　analyze　TAC.　In　this　work,　an　electrospinning-wet　chemistry-modification-calcination　method　was　proceed　to　fabricate　Co　doped　NiO　nanotubes　(Co-NiO　NT)　nanozyme.　Due　to　the　admirable　affinity,　fast　mass　and　electron　transfer　process　triggered　by　doping　of　Co　element　and　tubular　structure,　the　promoted　peroxidase-like　activity　of　Co-NiO　NT　was　realized.　Utilizing　its　notable　POD-like　(peroxidase-like)　activity,　a　quick,　convenient　and　accurate　colorimetric　method　of　AA　was　accomplished　with　the　detection　limit　of　7.7　nM　and　desirable　selectivity.　Furthermore,　the　TAC　analysis　of　fruits　juice　sample　was　demonstrated　and　verified　by　cupric　reducing　antioxidant　capacity　(CUPRAC)　approach,　indicating　the　accuracy,　convenience　and　reliability　of　the　Co-NiO　NT　based　colorimetric　sensing　system.　This　study　introduces　an　innovative　method　for　creating　high-efficiency　nanozymes　equipped　with　robust　TAC　analysis　functionalities,　aiming　to　tackle　complex　issues　in　the　prevention　and　monitoring　of　public　safety　and　food　industry.　©　2025　Elsevier　B.V.