TALEN-mediated　plant　genome　editing　has　the　advantages　of　high　specificity,　independence　from　epigenetic　modification,　lack　of　PAM　limitation,　and　feasibility　for　organelle　editing　due　to　no　need　for　RNA　binding.　However,　the　assembly　of　TALEN　vectors　was　difficult　and　laborious,　affecting　the　popularity　of　TALEN　genome　editing　in　plants.　In　this　study,　a　novel　TALEN-based　plant　genome　editing　tool,　ZQTALEN,　was　developed.　This　system　comprises　a　total　of　nine　plasmids　categorized　into　three　types.　The　TALE　repeat　units　are　obtained　through　PCR　utilizing　the　template　vector　as　the　amplification　template.　These　units　are　then　assembled　sequentially:　first　into　donor　vectors　to　form　entry　vectors,　and　subsequently　transferred　to　destination　vectors,　resulting　in　the　final　binary　vector.　Characterized　by　the　optimization　of　codon　usage,　assembly　method　of　TALE　repeat　array,　and　vector　backbone　components,　ZQTALEN　has　the　advantages　of　easy,　flexible　and　efficient　assembly　and　less　repeated　sequences　in　the　final　vector.　Using　the　ZQTALEN　system,　a　TALEN　binary　vector　was　successfully　constructed　to　target　the　endogenous　Nramp5　gene　in　rice,　resulting　in　the　high-frequency　acquisition　of　rice　mutants.　The　ZQTALEN　system　has　provided　a　versatile　tool　for　genetic　research　in　plants.　©　The　Author(s),　under　exclusive　licence　to　Springer　Nature　B.V.　2025.