The　persistence　of　T-2　toxin　in　food　and　feed　matrices　renders　it　a　pervasive　contaminant,　impacting　both　human　and　animal　health.　Traditional　detection　methods　suffer　from　cumbersome　instrumentation　and　intricate　procedures,　rendering　on-site　detection　of　T-2　toxin　unfeasible.　Therefore,　we　have　constructed　a　real-time　detection　method　for　T-2　toxin　detection　by　employing　a　target-responsive　DNA　hydrogel　in　conjunction　with　potassium　iodide　starch　test　paper.　This　method　integrates　both　colorimetric　and　distance-based　signal　outputs,　offering　a　streamlined　and　effective　approach　for　the　on-site　detection　of　T-2　toxin.　The　specific　binding　of　the　target　to　the　aptamer　in　the　DNA　hydrogel　results　in　the　collapse　of　the　hydrogels　structure,　which　changes　the　viscosity　of　the　system　and　released　horseradish　peroxidase　in　the　hydrogel　wrapped,　and　then　produces　blue-purple　marks　of　different　lengths　on　the　starch　iodide　papers　to　achieve　the　specific　detection　of　T-2　toxin.　Under　optimized　conditions,　the　assay　exhibits　a　detection　range　spanning　from　10　ng/mL　to　10　mg/mL　for　the　toxin,　with　a　detection　limit　of　12.83　ng/mL.　The　proposed　method　has　been　successfully　applied　for　the　detection　of　real　corn　samples　with　satisfied　result.　Such　colorimetric-distance　dual　signal　detection　method　offers　notable　advantages,　including　straightforward　operation,　clear　signal　interpretation,　and　practical　utility.　Its　implementation　enables　rapid,　on-the-spot　detection　of　T-2　toxin,　particularly　beneficial　in　resource-limited　regions　and　less　developed　countries.　©　2025　Elsevier　B.V.