Bletilla　striata,　a　medicinal　orchidaceous　plant,　is　recognized　for　its　significant　pharmacological　value.　However,　the　lack　of　comparative　metabolomic　data　across　different　extraction　methods　for　analyzing　its　bioactive　components　has　significantly　undervalued　the　application　potential　of　B.　striata　in　the　traditional　Chinese　medicine　market.　Using　six　ultrasound-assisted　extraction　methods　and　UPLC-MS/MS,　this　study　identified　1,945　metabolites　in　B.　striata　extracts.　The　dominant　categories　were　lipids　(51.35%),　flavonoids　(18.00%),　and　phenolic　acids　(12.51%).　KEGG　analysis　revealed　alterations　in　flavonoids　and　isoflavonoids　biosynthesis　pathways.　Thirteen　bitter　metabolites,　including　cinnamic　acid,　were　identified　in　B.　striata　tubers,　underscoring　their　potential　pharmacological　applications,　such　as　anti-inflammatory,　antioxidant　and　antibacterial　activities.　Optimizing　different　extraction　methods　can　better　preserve　the　bioactive　components　of　B.　striata　extracts,　thereby　enhancing　its　potential　applications　in　the　food　and　pharmaceutical　industries.　©　2025　The　Author(s)