To　rapidly　identify　the　volatile　markers　in　lotus　seeds,　this　research　compared　the　volatile　organic　compounds　(VOCs)　of　five　lotus　seed　samples　using　gas　chromatography-ion　mobility　spectroscopy　(GC-IMS)　and　chemometric　analysis.　The　results　revealed　that　49　VOCs　were　identified　from　the　lotus　seed　samples,　including　16　aldehydes,　15　alcohols,　8　ketones,　3　esters,　3　acids,　3　terpenes,　and　1　heterocyclic　compound,　respectively.　Among　these,　1-pentanol　M,　2-methylbutan-1-ol　M,　2-methylbutan-1-ol　D,　and　1-hexanol　M　were　identified　as　the　volatile　markers.　Based　on　the　VOC　analysis　using　GC-IMS,　effective　differentiation　of　lotus　seeds　was　achieved　through　chemometric　techniques,　such　as　principal　component　analysis　(PCA),　cluster　analysis　(CA),　and　partial　least　squares　discriminant　analysis　(PLS-DA).　2-furaldehyde　D,　hexanoic　acid　D,　and　1-propanol　M　were　found　to　be　the　most　important　components　contributing　to　the　differences　among　the　five　lotus　seed　samples.　This　research　demonstrates　that　GC-IMS　coupled　with　chemometric　analysis　provides　valuable　reference　information　for　the　identification　and　authenticity　evaluation　of　lotus　seeds,　helping　ensure　their　quality　on　the　market　and　offering　theoretical　support　for　their　identification　and　quality　assessment.　©　2025　Codon　Publications.