Wuyi　rock　tea　residue　is　rich　in　protein,　making　them　a　valuable　protein　source　for　producing　angiotensin　Iconverting　enzyme　(ACE)　inhibitory　peptides.　In　the　present　research,　five　novel　ACE　inhibitory　peptides　were　isolated　and　identified　from　protein　hydrolysate　of　Wuyi　rock　tea　residue　using　Sephadex　G-15,　reversed　phase　high-performance　liquid　chromatography　(RP-HPLC),　and　quadrupole　time-of-flight　mass　spectrometry.　The　peptide　sequences　were　identified　as　FPFPRPP,　PPPRGP,　PFPRPPH,　LGHPW　and　LKFPDF,　with　IC50　values　ranged　from　0.271　mg/mL　to　1.018　mg/mL,　with　FPFPRPP　demonstrating　the　strongest　activity.　As　indicated　by　the　Lineweaver-Burk　plot,　FPFPRPP　was　a　mixed-type　inhibitor　against　ACE.　Molecular　docking　studies　further　confirmed　that　FPFPRPP　forms　five　hydrogen　bonds　with　residues　near　the　active　sites　of　ACE,　with　a　binding　energy　of　-10.5　kcal/mol.　Functional　assay　in　human　umbilical　vein　endothelial　cells　(HUVECs)　showed　that　FPFPRPP　significantly　upregulated　nitric　oxide　production　and　downregulated　reactive　oxygen　species　and　endothelin-1　levels　induced　by　angiotensin　II,　indicating　its　potential　antihypertensive　effect.　These　findings　highlight　Wuyi　rock　tea　residue　as　a　promising　source　of　ACE　inhibitory　peptides　and　suggest　FPFPRPP　could　be　developed　as　a　functional　food　ingredient　with　possible　applications　in　hypertension　management.