Rabs　are　involved　in　neuronal　development　and　protrusion　formation.　Existing　studies　support　the　notion　that　manipulation　or　mutation　of　Rab　genes　could　lead　to　functional　changes　in　neurons.　However,　whether　Rabs　gene-manipulation　induced　Drosophila　eye-degeneration　remains　unknown.　By　down-regulating　Rab5,　but　not　Rab7,　we　first　constructed　a　compound　eye　injury　model　in　Drosophila.　As　the　distribution,　content,　and　even　maturation　of　Rab5-positive　endosomes　are　influenced　by　cytoskeletal　proteins,　like　actin　or　tubulin-related　proteins,　the　existence　of　a　bidirectional　regulatory　relationship　between　Rab5　and　the　cytoskeleton　remains　unclear　and　worth　researching.　Through　complete　transcriptome　sequencing　combined　immunofluorescence　testing,　we　confirmed　that　down-regulation　of　Rab5　affected　the　increase　of　α-Tub84B　(alternatively　named　TubA84B)　but　not　γ-tubulin.　Based　on　Weighted　Gene　Co-Expression　Network　Analysis　(WGCNA)　and　multi-tissue　screening　verification,　this　study　proposes　that　the　apoptosis-related　factors–Rab5–TubA84B　have　conserved　regulatory　functions　with　cooperative　expression.　Gene　manipulation　confirmed　that　apoptotic　factors,　especially　rpr,　strongly　regulate　Rab5,　and　may　ultimately　influence　microtubule　structure　through　complex　routes,　including　the　Rab5　variance　and　the　intracellular　configuration　ratio　of　α-Tubulin　to　Glyceraldehyde-3-phosphate　dehydrogenase.　©　2024