An　abnormal　level　of　alkaline　phosphatase　(ALP)　in　serum　is　related　to　many　diseases,　such　as　breast　cancer,　prostate　cancer,　hepatitis,　and　diabetes.　The　level　of　glucose　in　the　blood　is　related　to　diabetes　or　hypoglycemia.　Given　the　close　correlation　between　ALP　and　glucose　in　various　diseases,　it　is　essential　to　establish　an　accurate,　sensitive,　and　selective　assay　for　monitoring　the　levels　of　ALP　and　glucose　in　serum.　As　luminescent　materials,　upconversion　nanoparticles　(UCNPs)　stand　out　in　the　design　of　biosensors　because　of　their　high　photostability,　large　anti-Stokes　shifts　and　low　background　interference.　Additionally,　metal　organic　frameworks　(MOFs)　are　a　class　of　functional　porous　materials,　and　their　adjustable　pore　size　structure　and　high　specific　surface　area　expose　many　catalytic　sites,　making　MOFs　excellent　catalysts　and　ideal　materials　for　constructing　artificial　enzymes.　Herein,　a　fluorescent　and　colorimetric　dual-mode　probe　based　on　a　multifunctional　composite　(UCNP@MOF)　with　upconversion　luminescence　and　peroxidase-like　activity　was　proposed　for　the　detection　of　ALP　and　glucose.　Under　the　optimal　conditions,　the　detection　limits　of　ALP　and　glucose　by　the　fluorescence　method　were　0.046　U/L　and　0.079　μM,　respectively.　Furthermore,　the　method　was　used　to　determine　ALP　and　glucose　in　serum　samples,　and　the　detection　results　were　similar　to　those　of　commercial　kits;　moreover,　the　recoveries　were　in　the　range　of　92.7–105.4　%,　indicating　great　potential　for　accurate　and　sensitive　detection　of　ALP　and　glucose　in　biological　samples.　©　2024　Elsevier　B.V.