DNAzyme assisted single amplification for FEN1 activity detection using a personal glucose meter - Details

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Abstract：

Flap　endonuclease　1　(FEN1)　plays　a　vital　role　in　cancer　by　modulating　DNA　repair　mechanisms,　inducing　genomic　instability,　and　serving　as　a　promising　biomarker　for　cancer　diagnosis　and　prognosis.　In　this　work,　we　present　the　development　of　a　novel　DNAzyme　signal　amplification-directed　point-of-care　sensing　system　(Dz-PGM)　for　the　sensitive　and　specific　detection　of　FEN1.　The　Dz-PGM　system　utilizes　DNAzyme　signal　amplification　in　conjunction　with　a　personal　glucose　meter　(PGM)　for　reporting,　capitalizing　on　a　biochemical　cascade　initiated　by　FEN1　recognition.　Benefiting　from　enzymatic　recognition,　magnetic　separation,　and　DNAzyme　based　signal　amplification,　this　Dz-PGM　system　demonstrates　exceptional　specificity　and　achieves　a　low　detection　limit　of　3.56×　10−2　U/mL　for　FEN1.　The　versatility　of　the　system　is　further　demonstrated　through　its　ability　to　screen　FEN1　inhibitor　drugs　and　to　distinguish　FEN1　levels　in　real　cell　samples　with　high　accuracy.　The　article　underscores　the　potential　of　the　Dz-PGM　system　as　a　promising　tool　for　early　cancer　diagnosis,　and　in　constructing　sensitive　detection　platforms　for　DNA　repair　enzymes.　©　2024　Elsevier　B.V.

Keyword：

Enzymes Glucose Glucose sensors

Community：

[ 1 ] [Li, Mingxi]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China
[ 2 ] [Li, Mingxi]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 3 ] [Zang, Weijie]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China
[ 4 ] [Wang, Shuiliang]Fujian Key Laboratory of Aptamers Technology, Affiliated Dongfang Hospital (the 900th Hospital) of School of Medicine, Xiamen University, Fuzhou; 350025, China
[ 5 ] [Lin, Zhannuo]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 6 ] [Liu, Shu]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 7 ] [Chen, Yiling]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 8 ] [Ruan, Xiaohui]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 9 ] [Luo, Yuanfang]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 10 ] [Xing, Chao]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China
[ 11 ] [Xing, Chao]Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou; 350108, China
[ 12 ] [Lu, Chunhua]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China

Reprint 's Address：

[xing, chao]fujian key laboratory of functional marine sensing materials, center for advanced marine materials and smart sensors, college of materials and chemical engineering, minjiang university, fuzhou; 350108, china;;[xing, chao]moe key laboratory for analytical science of food safety and biology, fujian provincial key laboratory of analysis and detection technology for food safety, college of chemistry, fuzhou university, fuzhou; 350116, china;;

Email：

chunhualu@fzu.edu.cnchaoxing@mju.edu.cn

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Source ：

Analytica Chimica Acta

ISSN： 0003-2670

Year： 2025

Volume： 1336

5 . 7 0 0

JCR@2023

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 7

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