Triple　negative　breast　cancer　(TNBC)　seriously　endangers　women＇s　life　and　health　due　to　its　high　invasion　and　mortality.　Reactive　oxygen　species　(ROS)　mediated　tumor　cells　apoptosis　is　considered　an　effective　anticancer　approach.　Herein,　we　designed　a　natural　active　triple　helix　β-Glucan　(BFP)　wrapped　single　walled　carbon　nanotubes　(SWNTs)-loaded　doxorubicin　(DOX)　self-assembly　(BSD)　via　generating　excess　ROS　to　induce　oxidative　stress　damage　for　TNBC　therapy.　BSD　could　directly　consume　glutathione　(GSH)　to　promote　ROS.　In　vitro　results　demonstrated　that　BSD　exhibited　obvious　antitumor　effects　to　breast　cancer　cells　by　promoting　apoptosis.　Un-targeted　metabolomics　under　molecular　level　identified　the　specific　metabolic　targets　and　unveiled　that　BSD　markedly　disturbed　multiple　metabolic　pathways,　including　purine　metabolism,　pentose　phosphate　pathway,　glutathione　metabolism　pathways,　amino　sugar　and　nucleotide　sugar　metabolism　and　energy　metabolism,　led　to　the　inhibition　of　DNA　and　RNA　synthesis,　the　generation　of　ROS,　the　exacerbation　of　DNA　damage,　the　disruption　of　cell　membrane　integrity　and　the　decrease　of　ATP.　In　vitro　and　in　vivo　oxidative　stress　assays　further　verified　that　BSD　significantly　promoted　intracellular　oxidative　stress　and　resulted　in　cell　damage.　This　study　provides　theoretical　basis　for　the　development　and　screening　of　new　drugs　based　on　ROS　therapy　for　TNBC.　©　2024　Elsevier　B.V.