The　two-electron　oxygen　reduction　reaction　(2e-　ORR)　is　a　pivotal　pathway　for　the　distributed　production　of　hydrogen　peroxide　(H2O2).　In　nature,　enzymes　containing　manganese　(Mn)　centers　can　convert　reactive　oxygen　species　into　H2O2.　However,　Mn-based　heterogeneous　catalysts　for　2e-　ORR　are　scarcely　reported.　Herein,　we　developed　a　nature-inspired　single-atom　electrocatalyst　comprising　N,　O　co-coordinated　Mn　sites,　utilizing　carbon　dots　as　the　modulation　platform　(Mn　CD/C).　As-synthesized　Mn　CD/C　exhibited　exceptional　2e-　ORR　activity　with　an　onset　potential　of　0.786　V　and　a　maximum　H2O2　selectivity　of　95.8　%.　Impressively,　Mn　CD/C　continuously　produced　0.1　M　H2O2　solution　at　200　mA/cm2　for　50　h　in　the　flow　cell,　with　negligible　loss　in　activity　and　H2O2　faradaic　efficiency,　demonstrating　practical　application　potential.　The　enhanced　activity　was　attributed　to　the　incorporation　of　Mn　atomic　sites　into　the　carbon　dots.　Theoretical　calculations　revealed　that　the　N,　O　co-coordinated　structure,　combined　with　abundant　oxygen-containing　functional　groups　on　the　carbon　dots,　optimized　the　binding　strength　of　intermediate　*OOH　at　the　Mn　sites　to　the　apex　of　the　catalytic　activity　volcano.　This　work　illustrates　that　carbon　dots　can　serve　as　a　versatile　platform　for　modulating　the　microenvironment　of　single-atom　catalysts　and　for　the　rational　design　of　nature-inspired　catalysts.