Ganoderma　lucidum,　used　in　East　Asia　for　its　health　benefits,　contains　ganoderic　acids　(GA)　which　have　various　pharmacological　activities　but　are　limited　by　poor　water　solubility　and　low　oral　bioaccessibility.　This　study　synthesized　and　characterized　ganoderic　acids　loaded　zein-chitosan　nanoparticles　(GA-NPs),　and　investigated　its　advantages　in　alleviating　alcoholic　liver　injury　(ALI)　in　mice　model.　The　GA-NPs　demonstrated　high　encapsulation　efficiency　(92.68%),　small　particle　size　(177.20　nm),　and　a　+29.53　mV　zeta　potential.　The　experimental　results　of　alcohol-induced　liver　injury　mouse　model　showed　that　GA-NPs　significantly　improved　liver　metabolic　function,　reduced　alcohol-induced　liver　oxidative　stress　in　liver　by　decreasing　lactate　dehydrogenase　activity　and　malondialdehyde　level,　while　increasing　the　activities　of　liver　antioxidant　enzymes　and　alcohol　dehydrogenase.　Moreover,　GA-NPs　were　favorable　to　ameliorate　intestinal　microbiota　dysbiosis　in　mice　exposed　to　alcohol　by　increasing　the　proportion　of　probiotics　such　as　Romboutsia,　Faecalibaculum,　Bifidobacterium　and　Turicibacter,　etc.,　which　were　highly　correlated　with　the　improvement　of　liver　function.　Furthermore,　GA-NPs　modulated　the　mRNA　expression　related　to　ethanol　metabolism,　oxidative　stress　and　lipid　metabolism.　Conclusively,　this　study　revealed　that　GA-NPs　have　stronger　hepatoprotective　effects　than　non-encapsulated　ganoderic　acids　on　alleviating　ALI　by　regulating　intestinal　microbiota　and　liver　metabolism.