This　study　presents　a　portable　EEG　signal　acquisition　board　with　16　channels,　the　firmware　development　of　the　ESP32　module　enables　the　transmission　of　the　EEG　signals　acquired　by　the　analog　front-end　ADS1299　to　the　host　computer　using　the　SPI　communication　interface.　The　ESP32　module　has　built-in　Bluetooth　and　Wi-Fi　communication　peripherals,　which　enable　fast,　high-quality　transmission　of　EEG　signals.　This　study　realized　a　16-channel　data　acquisition　system,　and　measured　the　shorting　noise　of　the　16　channels　which　obtains　an　average　noise　of　1.782uV.　The　experimental　results　show　that　this　study　has　a　better　recognition　effect　for　identifying　the　four　SSVEP　signals.　The　average　recognition　accuracy　of　five　subjects　using　the　FFT　and　the　CCA　algorithms　were　82%　and　89.5%　respectively.　©　2024　IEEE.