As　digital　data　undergo　explosive　growth,　deoxyribonucleic　acid　(DNA)　has　emerged　as　a　promising　storage　medium　due　to　its　high　density,　longevity,　and　ease　of　replication,　offering　vast　potential　in　data　storage　solutions.　This　study　focuses　on　the　protection　and　retrieval　of　data　during　the　DNA　storage　process,　developing　a　technique　that　employs　flow　cytometry　sorting　(FCS)　to　segregate　multicolored　fluorescent　DNA　microparticles　encoded　with　data　and　facilitating　efficient　random　access.　Moreover,　the　encapsulated　fluorescent　DNA　microparticles,　formed　through　layer-by-layer　self-assembly,　preserve　structural　and　sequence　integrity　even　under　harsh　conditions　while　also　supporting　a　high-density　DNA　payload.　Experimental　results　have　shown　that　the　encoded　data　can　still　be　successfully　recovered　from　encapsulated　DNA　microparticles　following　de-encapsulation.　We　also　successfully　demonstrated　the　automated　encapsulation　process　of　fluorescent　DNA　microparticles　using　a　microfluidic　chip.　This　research　provides　an　innovative　approach　to　the　long-term　stability　and　random　readability　of　DNA　data　storage.　©　2024　American　Chemical　Society.