In　this　work,　an　electrochemiluminescence　(ECL)　biosensor　for　T-2　toxin　detection　has　been　designed　by　combining　the　high　specificity　of　a　DNA　hydrogel　system　with　the　convenience　and　sensitivity　of　homogeneous　ECL　detection.　Specifically,　A　hydrogel　was　engineered　through　the　reaction　between　the　carboxyl　group　of　hyaluronic　acid　(HA)　and　the　amino　groups　of　both　polyethyleneimine　(PEI)　and　DNA-modified　strands,　resulting　in　a　three-dimensional　network　that　encapsulates　glucose　oxidase.　Glucose　oxidase　(GOD)　is　capable　of　catalyzing　the　conversion　of　glucose　into　hydrogen　peroxide　(H₂O₂),　which　is　a　co-reactant　necessary　for　the　chemiluminescent　reagent　luminol　to　emit　light.　Upon　the　presence　of　the　target　analyte　T-2　toxin,　the　aptamer　recognizes　and　binds　to　it,　leading　to　the　dissociation　and　cleavage　of　the　hydrogel　framework.　This　process　results　in　the　leakage　of　the　encapsulated　GOD　into　the　supernatant.　Subsequently,　Adding　the　collected　supernatant　to　a　phosphate-buffered　saline　(PBS)　solution　containing　glucose　and　luminol　for　ECL　testing　yields　a　noticeable　signal　change.　This　sensor　eliminates　the　need　for　electrode　modification,　simplifying　the　operation.　Within　a　concentration　range　of　10　fg/mL　to　100　ng/mL,　the　ECL　signal　intensity　exhibits　an　excellent　linear　relationship　with　the　logarithm　of　the　target　concentration,　with　a　detection　limit　as　low　as　0.32　fg/mL.　The　method　also　demonstrates　excellent　reproducibility　and　stability.　The　sensor　demonstrated　precise　detection　capabilities　in　actual　milk　and　beer　samples,　a　crucial　aspect　for　research　focused　on　specific　targets.　©　2024