Environmental　regulation　promotes　industrial　structure　change　and　regional　economic　transformation　through　land　use　adjustment,　which　gets　a　new　way　to　explore　the　path　of　reforming　traditional　industrialization　and　urbanization.　Based　on　the　panel　data　of　128　prefecture-level　cities　in　China　＇s　Yangtze　River　Economic　Belt　from　2000　to　2020,　this　paper　uses　the　spatial　Dubin　model　to　analyze　the　impact　of　environmental　regulation　and　land　use　efficiency　on　the　upgrading　of　industrial　structure,　and　sets　the　panel　threshold　model　to　examine　the　impact　of　environmental　regulation　on　the　upgrading　of　industrial　structure　by　affecting　land　use　efficiency.　The　results　show　that　formal　environmental　regulation　has　a　significant　positive　spatial　effect　on　the　rationalization　and　upgrading　of　industrial　structure,　which　are　0.1734　and　0.2854　respectively.　Informal　environmental　regulation　has　a　negative　spillover　effect　on　neighboring　provinces　but　not　significant.　Heterogeneous　environmental　regulation　has　obvious　＂double　threshold　effect＂　on　industrial　upgrading　by　affecting　land　use　efficiency.　When　the　threshold　of　environmental　regulation　intensity　is　0.0315-0.0886,　environmental　regulation　still　inhibits　land　use　efficiency　and　industrial　structure　upgrading.　When　the　threshold　value　is　greater　than　0.0886,　environmental　regulation　has　a　positive　impact　on　land　use　efficiency　but　not　significant.　With　the　intensity　of　environmental　regulation　from　weak　to　strong,　it　will　produce　a　double　threshold　effect　of　＂strong　inhibition-weak　inhibition-interaction　promotion＂　on　the　upgrading　of　manufacturing　structure　through　the　adjustment　of　land　use　efficiency.